Click here to switch to the map view.

The map label for this gene is blaI

Identifier: 170755728

GI number: 170755728

Start: 797005

End: 797385

Strand: Direct

Name: blaI

Synonym: CLD_0087

Alternate gene names: 170755728

Gene position: 797005-797385 (Clockwise)

Preceding gene: 170757796

Following gene: 170757428

Centisome position: 20.14

GC content: 32.02

Gene sequence:

>381_bases
ATGAAAGATATGCCAAAAATCTCAGCGGCTGAGTGGGAAGTAATGAAACTTCTTTGGAAAGAGAGTCCTTTAACATCTGA
AAAAATAATTAATTCACTAACAGAAAAAATGGATTGGTCAACGCAAACTGTAAAGACATTTATAACTAGACTTTTGAAAA
AAGAAGCAATAGGTTTTGAAAAAGTAGGTAGAGCTTATAATTATTATCCATTAATATCCGAAGATGAATGTGTAAAAGCT
GAAAATCAATCTTTTCTTCAAAAGGTATACGATGGAGCTGTAGGTATGCTCTTTACAAGGTTTTTAGAGGAAGAGACTTT
GTCTGAAGAGGAAATTGAAGAGCTAGAGCAAATTCTTAAAGATAAGAAAGAAGGAAAGTAA

Upstream 100 bases:

>100_bases
GTACGTAAATTATGGAATTTTTAACTTGACAATGATAGTTTACAGTTGTAGACTGTTATTGTAGATCTACAATTGTAAAC
TTAAAATGGAGGAATAAGAT

Downstream 100 bases:

>100_bases
GGTTAGGCTTTTGTAATTGAAAATGGGAGGATATTAAATATGGATATCTTAGTCAAAATTTTTTTATGGGTTCTACGCGC
TTCATTAACAGCAAGCATTG

Product: penicillinase repressor

Products: NA

Alternate protein names: Beta-lactamase repressor protein; Regulatory protein BlaI [H]

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MKDMPKISAAEWEVMKLLWKESPLTSEKIINSLTEKMDWSTQTVKTFITRLLKKEAIGFEKVGRAYNYYPLISEDECVKA
ENQSFLQKVYDGAVGMLFTRFLEEETLSEEEIEELEQILKDKKEGK

Sequences:

>Translated_126_residues
MKDMPKISAAEWEVMKLLWKESPLTSEKIINSLTEKMDWSTQTVKTFITRLLKKEAIGFEKVGRAYNYYPLISEDECVKA
ENQSFLQKVYDGAVGMLFTRFLEEETLSEEEIEELEQILKDKKEGK
>Mature_126_residues
MKDMPKISAAEWEVMKLLWKESPLTSEKIINSLTEKMDWSTQTVKTFITRLLKKEAIGFEKVGRAYNYYPLISEDECVKA
ENQSFLQKVYDGAVGMLFTRFLEEETLSEEEIEELEQILKDKKEGK

Specific function: Transcriptional repressor that constitutively blocks expression of beta-lactamase. Regulates genes involved in antibiotic resistance. Binds DNA as a dimer [H]

COG id: COG3682

COG function: function code K; Predicted transcriptional regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BlaI transcriptional regulatory family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005650
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03965 Pencillinase_R [H]

EC number: NA

Molecular weight: Translated: 14736; Mature: 14736

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDMPKISAAEWEVMKLLWKESPLTSEKIINSLTEKMDWSTQTVKTFITRLLKKEAIGFE
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHH
KVGRAYNYYPLISEDECVKAENQSFLQKVYDGAVGMLFTRFLEEETLSEEEIEELEQILK
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
DKKEGK
HHCCCC
>Mature Secondary Structure
MKDMPKISAAEWEVMKLLWKESPLTSEKIINSLTEKMDWSTQTVKTFITRLLKKEAIGFE
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHH
KVGRAYNYYPLISEDECVKAENQSFLQKVYDGAVGMLFTRFLEEETLSEEEIEELEQILK
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
DKKEGK
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3096969; 3305074; 3260234; 3040663; 3498148 [H]