Click here to switch to the map view.

The map label for this gene is rfaD [C]

Identifier: 170755592

GI number: 170755592

Start: 2858117

End: 2859034

Strand: Reverse

Name: rfaD [C]

Synonym: CLD_1886

Alternate gene names: 170755592

Gene position: 2859034-2858117 (Counterclockwise)

Preceding gene: 170754404

Following gene: 170756889

Centisome position: 72.23

GC content: 28.32

Gene sequence:

>918_bases
ATGAAAATATTAGTTACTGGTGGCGCAGGATTTATAGGATCGAATTTAGTAGATAAATTTATAGGTATGGGAAATGATGT
ATGTATAATTGATAATTTATCCACTGGAAATATAAATAATGTAAATAAAAAAGCTAGATTATATATAAATGATATATTGG
ATTCTAATATAGCTAACATATTTAAAAAAGAAAAGTTTGATATAGTTTATCATTTTGCTGCTCAAATCGATGTACAAAAA
TCCATAAAAGATCCTATGTTTGATTCTAATGTAAATATATGTGGCACCGTGAATATATTAAAAAGTTGTGTTGATTATGG
GGTTAAGAAGATAATATATCCCTCTTCAGCAGCAGTTTATGGACAACCAGAATATTTACCTATAGATGAAAAACATAGGG
TAAAGCCCATATCTTCTTATGGATTATCAAAATATACACCAGAAGAATATATAAGATCCTTTAGTGAATTATATAACTTG
GATTATACCATATTTAGATATGCTAATGTTTATGGTATAAGACAGGATCCTAAGGGTGAAGGTGGAGTAGTATCTATATT
TATGGATAGATTATTTAAAAATTATCCCCTATGCATATTCGGAGATGGAAAGGCTTTGAGAGATTATATATATGTAGAAG
ATGTGGTAAATGCTAATATTGCCGCCTTATATAATGGTAGTAGAAATCTATTTAATATAGGTACCGGTGTGTATACTACT
GTTAAAGATTTAGCTCAAATGATGATAGACACTATGAAAGTTCAATCTCATATAGAGTACCAAGAGGCTAGAAAAGGAGA
TATAGAGAAATCTCATTTTAATATAGAAAAAGCTAAAGTGGAACTTAAGTGGGAACCAAAATATAATTTACAAAAGGGGC
TCATAAAAACTATAAATTATTATAAAGACAATATATAG

Upstream 100 bases:

>100_bases
GATAGGATATTAGGTAGACAAGCAAAAGTTAATATAGAAGAGGATACCATATTAACTTGGGAATTATTTTAATTATTATT
TTAAAAGAGGATGATTAACT

Downstream 100 bases:

>100_bases
GATAATGCTTATTTAAAAGTATTACATAAGGTGATAAATATGTTAACAAAATTTAAAAATATATTTAAAAACAATAAGTT
TAATGTGATTTTCAAATATT

Product: NAD-dependent epimerase/dehydratase family protein

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKILVTGGAGFIGSNLVDKFIGMGNDVCIIDNLSTGNINNVNKKARLYINDILDSNIANIFKKEKFDIVYHFAAQIDVQK
SIKDPMFDSNVNICGTVNILKSCVDYGVKKIIYPSSAAVYGQPEYLPIDEKHRVKPISSYGLSKYTPEEYIRSFSELYNL
DYTIFRYANVYGIRQDPKGEGGVVSIFMDRLFKNYPLCIFGDGKALRDYIYVEDVVNANIAALYNGSRNLFNIGTGVYTT
VKDLAQMMIDTMKVQSHIEYQEARKGDIEKSHFNIEKAKVELKWEPKYNLQKGLIKTINYYKDNI

Sequences:

>Translated_305_residues
MKILVTGGAGFIGSNLVDKFIGMGNDVCIIDNLSTGNINNVNKKARLYINDILDSNIANIFKKEKFDIVYHFAAQIDVQK
SIKDPMFDSNVNICGTVNILKSCVDYGVKKIIYPSSAAVYGQPEYLPIDEKHRVKPISSYGLSKYTPEEYIRSFSELYNL
DYTIFRYANVYGIRQDPKGEGGVVSIFMDRLFKNYPLCIFGDGKALRDYIYVEDVVNANIAALYNGSRNLFNIGTGVYTT
VKDLAQMMIDTMKVQSHIEYQEARKGDIEKSHFNIEKAKVELKWEPKYNLQKGLIKTINYYKDNI
>Mature_305_residues
MKILVTGGAGFIGSNLVDKFIGMGNDVCIIDNLSTGNINNVNKKARLYINDILDSNIANIFKKEKFDIVYHFAAQIDVQK
SIKDPMFDSNVNICGTVNILKSCVDYGVKKIIYPSSAAVYGQPEYLPIDEKHRVKPISSYGLSKYTPEEYIRSFSELYNL
DYTIFRYANVYGIRQDPKGEGGVVSIFMDRLFKNYPLCIFGDGKALRDYIYVEDVVNANIAALYNGSRNLFNIGTGVYTT
VKDLAQMMIDTMKVQSHIEYQEARKGDIEKSHFNIEKAKVELKWEPKYNLQKGLIKTINYYKDNI

Specific function: Biosynthesis Of The Lipopolysaccharide Precursor ADP-L- Glycerol-D-Mannoheptose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=309, Percent_Identity=28.4789644012945, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI56237023, Length=338, Percent_Identity=30.7692307692308, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI56118217, Length=338, Percent_Identity=30.7692307692308, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI189083684, Length=338, Percent_Identity=30.7692307692308, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI7657641, Length=321, Percent_Identity=32.0872274143302, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI193211614, Length=237, Percent_Identity=26.5822784810127, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI8393516, Length=237, Percent_Identity=26.5822784810127, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1790049, Length=274, Percent_Identity=31.3868613138686, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788353, Length=340, Percent_Identity=28.8235294117647, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786974, Length=316, Percent_Identity=28.4810126582279, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI48994969, Length=335, Percent_Identity=27.4626865671642, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788589, Length=335, Percent_Identity=22.9850746268657, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788365, Length=328, Percent_Identity=20.4268292682927, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788366, Length=276, Percent_Identity=23.1884057971014, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17539532, Length=310, Percent_Identity=28.0645161290323, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71982035, Length=345, Percent_Identity=28.695652173913, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71982038, Length=347, Percent_Identity=28.5302593659942, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI115532424, Length=321, Percent_Identity=27.4143302180685, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17568069, Length=323, Percent_Identity=28.4829721362229, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319493, Length=328, Percent_Identity=32.3170731707317, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21356223, Length=309, Percent_Identity=28.8025889967638, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19923002, Length=339, Percent_Identity=28.6135693215339, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 34677; Mature: 34677

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSNLVDKFIGMGNDVCIIDNLSTGNINNVNKKARLYINDILDSNIANI
CEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHEEEEHHHHHHHHHHH
FKKEKFDIVYHFAAQIDVQKSIKDPMFDSNVNICGTVNILKSCVDYGVKKIIYPSSAAVY
HHHHHHHEEEEEHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHCHHEEECCCCCEEC
GQPEYLPIDEKHRVKPISSYGLSKYTPEEYIRSFSELYNLDYTIFRYANVYGIRQDPKGE
CCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEHEEEECCCCCCC
GGVVSIFMDRLFKNYPLCIFGDGKALRDYIYVEDVVNANIAALYNGSRNLFNIGTGVYTT
CCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCHHHH
VKDLAQMMIDTMKVQSHIEYQEARKGDIEKSHFNIEKAKVELKWEPKYNLQKGLIKTINY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEEEEEEECCCCCHHHHHHHHHHH
YKDNI
HHCCC
>Mature Secondary Structure
MKILVTGGAGFIGSNLVDKFIGMGNDVCIIDNLSTGNINNVNKKARLYINDILDSNIANI
CEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHEEEEHHHHHHHHHHH
FKKEKFDIVYHFAAQIDVQKSIKDPMFDSNVNICGTVNILKSCVDYGVKKIIYPSSAAVY
HHHHHHHEEEEEHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHCHHEEECCCCCEEC
GQPEYLPIDEKHRVKPISSYGLSKYTPEEYIRSFSELYNLDYTIFRYANVYGIRQDPKGE
CCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEHEEEECCCCCCC
GGVVSIFMDRLFKNYPLCIFGDGKALRDYIYVEDVVNANIAALYNGSRNLFNIGTGVYTT
CCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCHHHH
VKDLAQMMIDTMKVQSHIEYQEARKGDIEKSHFNIEKAKVELKWEPKYNLQKGLIKTINY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEEEEEEECCCCCHHHHHHHHHHH
YKDNI
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]