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The map label for this gene is prfA

Identifier: 170755459

GI number: 170755459

Start: 176406

End: 177482

Strand: Direct

Name: prfA

Synonym: CLD_0647

Alternate gene names: 170755459

Gene position: 176406-177482 (Clockwise)

Preceding gene: 170756524

Following gene: 170754858

Centisome position: 4.46

GC content: 31.66

Gene sequence:

>1077_bases
ATGTTAGAAAGACTTAATTTTATAGAAAATAAATATGAAGAACTATCAAATAAAATAAGCGACCCTTCAGTTATGGCAAA
TCAAAAAGAATGGCAAAAACTTTGTAAAGAGCATGCTGATTTAGAAATTATAGTTAATACATATAGAGAGTATAAAAAGG
CACAAGAGGATTTAGAATCAGATAAAGAAATGCTAAAGGAAGAATCAGACAAAGAACTAAGAGAAATGGCACAGGAAGAA
ATAAAAGAGTTAACTTTAAAATTAGAAGATTTAGAAAGAGAATTAACTATATTATTACTACCGAAAGATCCTAATGATGA
TAAGGATGTATTTATAGAAATTAGAGCAGGTGCTGGTGGGGAAGAGGCGGCTTTATTTGCTTCTAACTTATTAAGAATGT
ATACAAGATATGCAGAAAGAAAAAATTGGAAAGTGGAAACTATGAGTTTAAATGCTACAGATATAGGCGGATTTAAAGAA
GTTACTGTAGCAATTAAAGGAAAGGGAGCTTATAGTAGATTAAAATATGAAAGTGGAGTTCACAGAGTTCAAAGGGTTCC
AGATACAGAATCAAGCGGTAGAATTCACACTTCTACAGCTACTGTAGCAGTACTTCCTGAAGTAGATGATGTAGATATAA
ATATAAATGCTAATGATTTAAGAATTGATGTTTATAGAGCGTCAGGACATGGTGGACAGTGTGTAAATACTACAGATTCA
GCTGTAAGAATAACTCACTTACCAACAGGCCTTGTAGTTACATGTCAAGATGAAAAATCACAATTAAAAAATAAAGAAAA
AGCAATGAAAGTTTTAAAAGCTAGATTGTTTGAAGCTGCAGAGGCTGAAAGAGCAGCATCTATAGCAGAAGATAGAAAAA
GCCAAGTTGGAACTGGAGATAGAAGTGAAAGAATAAGAACTTATAACTATCCTCAAGGAAGAATTACGGATCATAGAATA
GGTTTAACATTATATAAATTAGAAACTTTTTTAGACGGGGATATAGATGAAGTTATAGAAGCTTTAGTAACAGAAGATCA
AGCGGAAAAGATGAAAGATTTGGGTAGAGTTAATTAA

Upstream 100 bases:

>100_bases
AAAAGCTAGAAAGTATTAAAATTCACAGGTTAGTTGTAAATGCTTTATAAAGTATGATATAATATAAAATTGTGAAAATA
TATATACGGAGTGAGAAATA

Downstream 100 bases:

>100_bases
TATTAATTAAGGAAAGAAGAGGTAATTATGGATTTAAACAAAGGCCGTAGAAATCAAAAGAGATCTTATGAAAGATTTGT
TGTGATTATGAGCTTTATTT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MLERLNFIENKYEELSNKISDPSVMANQKEWQKLCKEHADLEIIVNTYREYKKAQEDLESDKEMLKEESDKELREMAQEE
IKELTLKLEDLERELTILLLPKDPNDDKDVFIEIRAGAGGEEAALFASNLLRMYTRYAERKNWKVETMSLNATDIGGFKE
VTVAIKGKGAYSRLKYESGVHRVQRVPDTESSGRIHTSTATVAVLPEVDDVDININANDLRIDVYRASGHGGQCVNTTDS
AVRITHLPTGLVVTCQDEKSQLKNKEKAMKVLKARLFEAAEAERAASIAEDRKSQVGTGDRSERIRTYNYPQGRITDHRI
GLTLYKLETFLDGDIDEVIEALVTEDQAEKMKDLGRVN

Sequences:

>Translated_358_residues
MLERLNFIENKYEELSNKISDPSVMANQKEWQKLCKEHADLEIIVNTYREYKKAQEDLESDKEMLKEESDKELREMAQEE
IKELTLKLEDLERELTILLLPKDPNDDKDVFIEIRAGAGGEEAALFASNLLRMYTRYAERKNWKVETMSLNATDIGGFKE
VTVAIKGKGAYSRLKYESGVHRVQRVPDTESSGRIHTSTATVAVLPEVDDVDININANDLRIDVYRASGHGGQCVNTTDS
AVRITHLPTGLVVTCQDEKSQLKNKEKAMKVLKARLFEAAEAERAASIAEDRKSQVGTGDRSERIRTYNYPQGRITDHRI
GLTLYKLETFLDGDIDEVIEALVTEDQAEKMKDLGRVN
>Mature_358_residues
MLERLNFIENKYEELSNKISDPSVMANQKEWQKLCKEHADLEIIVNTYREYKKAQEDLESDKEMLKEESDKELREMAQEE
IKELTLKLEDLERELTILLLPKDPNDDKDVFIEIRAGAGGEEAALFASNLLRMYTRYAERKNWKVETMSLNATDIGGFKE
VTVAIKGKGAYSRLKYESGVHRVQRVPDTESSGRIHTSTATVAVLPEVDDVDININANDLRIDVYRASGHGGQCVNTTDS
AVRITHLPTGLVVTCQDEKSQLKNKEKAMKVLKARLFEAAEAERAASIAEDRKSQVGTGDRSERIRTYNYPQGRITDHRI
GLTLYKLETFLDGDIDEVIEALVTEDQAEKMKDLGRVN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=284, Percent_Identity=48.5915492957746, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI34577120, Length=341, Percent_Identity=41.3489736070381, Blast_Score=258, Evalue=4e-69,
Organism=Homo sapiens, GI166795305, Length=189, Percent_Identity=47.0899470899471, Blast_Score=194, Evalue=1e-49,
Organism=Escherichia coli, GI1787462, Length=355, Percent_Identity=51.5492957746479, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=41.9161676646707, Blast_Score=235, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI17542784, Length=286, Percent_Identity=37.7622377622378, Blast_Score=195, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=329, Percent_Identity=46.5045592705167, Blast_Score=279, Evalue=6e-76,
Organism=Drosophila melanogaster, GI19921226, Length=338, Percent_Identity=44.3786982248521, Blast_Score=257, Evalue=9e-69,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40659; Mature: 40659

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERLNFIENKYEELSNKISDPSVMANQKEWQKLCKEHADLEIIVNTYREYKKAQEDLES
CCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHH
DKEMLKEESDKELREMAQEEIKELTLKLEDLERELTILLLPKDPNDDKDVFIEIRAGAGG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEECCCCC
EEAALFASNLLRMYTRYAERKNWKVETMSLNATDIGGFKEVTVAIKGKGAYSRLKYESGV
CHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHH
HRVQRVPDTESSGRIHTSTATVAVLPEVDDVDININANDLRIDVYRASGHGGQCVNTTDS
HHHHCCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCEEEEEEEEECCCCCCEECCCCC
AVRITHLPTGLVVTCQDEKSQLKNKEKAMKVLKARLFEAAEAERAASIAEDRKSQVGTGD
EEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSERIRTYNYPQGRITDHRIGLTLYKLETFLDGDIDEVIEALVTEDQAEKMKDLGRVN
CCCCCEECCCCCCCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCCC
>Mature Secondary Structure
MLERLNFIENKYEELSNKISDPSVMANQKEWQKLCKEHADLEIIVNTYREYKKAQEDLES
CCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHH
DKEMLKEESDKELREMAQEEIKELTLKLEDLERELTILLLPKDPNDDKDVFIEIRAGAGG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEECCCCC
EEAALFASNLLRMYTRYAERKNWKVETMSLNATDIGGFKEVTVAIKGKGAYSRLKYESGV
CHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHH
HRVQRVPDTESSGRIHTSTATVAVLPEVDDVDININANDLRIDVYRASGHGGQCVNTTDS
HHHHCCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCEEEEEEEEECCCCCCEECCCCC
AVRITHLPTGLVVTCQDEKSQLKNKEKAMKVLKARLFEAAEAERAASIAEDRKSQVGTGD
EEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSERIRTYNYPQGRITDHRIGLTLYKLETFLDGDIDEVIEALVTEDQAEKMKDLGRVN
CCCCCEECCCCCCCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA