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The map label for this gene is hemK [H]

Identifier: 170756524

GI number: 170756524

Start: 175473

End: 176324

Strand: Direct

Name: hemK [H]

Synonym: CLD_0648

Alternate gene names: 170756524

Gene position: 175473-176324 (Clockwise)

Preceding gene: 170757760

Following gene: 170755459

Centisome position: 4.43

GC content: 26.41

Gene sequence:

>852_bases
TTGATATTAAAAGATTTACTAATAGAAGGATATGGTGTTTTAAAAAAAGCATCTATAGGTAGCTATCAAATAGATGCTCA
ACTTTTATTAGGAAAAGTATTAAAAAAGGATAGATTGTTCATTTTGACTAATCCTGATTACCATATAAAAGAGGAAGAGA
AAGAAAAATATTTTCAATTAATAGACCTTAGAAAAGATAAAATGCCTATAAAATATATATTAGGTACAACTGAATTTATG
GGACTAGATTTTAACATAAAAAAGGGTGTATTAATACCAAGACCAGATACAGAAATATTAGTAGAAACGGTTTTAGAAGA
AATAAAGAATAAAAATTATAAGCAAATTTGTGATGTTTGTTGTGGTAGTGGTATTATAGGAATTACTATAGGATATACCT
TAAATAATACAGAAATAATTTGCTATGATATAGAAGATATTCCATACAATACTACTAAAGAAAATATACTCAAGCATAAT
CTTGAAGATAGAGTTAAAGTTTTAAAAAGTGATTTACTTACAGAGGCAATAAAAGAAAAAAGAAGGTTTGATCTTATAGT
ATCTAATCCACCATATATAAGAGAAGATGTTATTGAAACACTTATGGATGATGTTAAAAAATATGAACCCTTTGAAGCTT
TATGTGGTGGAAAAGATGGATTATTTTTTTATGAGGGTATAATAAAGCAAAGTTTAGAAGTGCTAAATGATGGGGGAACC
ATAGCTTTTGAAATAGGTTATGATCAAAAAATACAGGTTTCTCACATTTTAAATGAATATGGTTTTAAGGATGTGTTATG
TATAAAAGACTTAGCTGGAAAAGACAGAGTTATAAAAGCTAGAAAGTATTAA

Upstream 100 bases:

>100_bases
AAACTAACAACTAAAGAACCTGACTTAAGGCAATTGGAAGTAGCTATAATGGCTTTAAAAGCAGCAGAAGGAATTGAAAG
TTCAATGGAGTGATGTTAAT

Downstream 100 bases:

>100_bases
AATTCACAGGTTAGTTGTAAATGCTTTATAAAGTATGATATAATATAAAATTGTGAAAATATATATACGGAGTGAGAAAT
AATGTTAGAAAGACTTAATT

Product: HemK family modification methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MILKDLLIEGYGVLKKASIGSYQIDAQLLLGKVLKKDRLFILTNPDYHIKEEEKEKYFQLIDLRKDKMPIKYILGTTEFM
GLDFNIKKGVLIPRPDTEILVETVLEEIKNKNYKQICDVCCGSGIIGITIGYTLNNTEIICYDIEDIPYNTTKENILKHN
LEDRVKVLKSDLLTEAIKEKRRFDLIVSNPPYIREDVIETLMDDVKKYEPFEALCGGKDGLFFYEGIIKQSLEVLNDGGT
IAFEIGYDQKIQVSHILNEYGFKDVLCIKDLAGKDRVIKARKY

Sequences:

>Translated_283_residues
MILKDLLIEGYGVLKKASIGSYQIDAQLLLGKVLKKDRLFILTNPDYHIKEEEKEKYFQLIDLRKDKMPIKYILGTTEFM
GLDFNIKKGVLIPRPDTEILVETVLEEIKNKNYKQICDVCCGSGIIGITIGYTLNNTEIICYDIEDIPYNTTKENILKHN
LEDRVKVLKSDLLTEAIKEKRRFDLIVSNPPYIREDVIETLMDDVKKYEPFEALCGGKDGLFFYEGIIKQSLEVLNDGGT
IAFEIGYDQKIQVSHILNEYGFKDVLCIKDLAGKDRVIKARKY
>Mature_283_residues
MILKDLLIEGYGVLKKASIGSYQIDAQLLLGKVLKKDRLFILTNPDYHIKEEEKEKYFQLIDLRKDKMPIKYILGTTEFM
GLDFNIKKGVLIPRPDTEILVETVLEEIKNKNYKQICDVCCGSGIIGITIGYTLNNTEIICYDIEDIPYNTTKENILKHN
LEDRVKVLKSDLLTEAIKEKRRFDLIVSNPPYIREDVIETLMDDVKKYEPFEALCGGKDGLFFYEGIIKQSLEVLNDGGT
IAFEIGYDQKIQVSHILNEYGFKDVLCIKDLAGKDRVIKARKY

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=296, Percent_Identity=29.7297297297297, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1787463, Length=263, Percent_Identity=31.1787072243346, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI87082085, Length=213, Percent_Identity=27.6995305164319, Blast_Score=95, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324266, Length=161, Percent_Identity=31.6770186335404, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24582226, Length=253, Percent_Identity=28.0632411067194, Blast_Score=107, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32548; Mature: 32548

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKDLLIEGYGVLKKASIGSYQIDAQLLLGKVLKKDRLFILTNPDYHIKEEEKEKYFQL
CCHHHHHHHCCCHHEECCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
IDLRKDKMPIKYILGTTEFMGLDFNIKKGVLIPRPDTEILVETVLEEIKNKNYKQICDVC
HHHHCCCCCEEEEECCHHHEECCEECCCCEEECCCCHHHHHHHHHHHHHCCCHHHHHHHH
CGSGIIGITIGYTLNNTEIICYDIEDIPYNTTKENILKHNLEDRVKVLKSDLLTEAIKEK
HCCCEEEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHH
RRFDLIVSNPPYIREDVIETLMDDVKKYEPFEALCGGKDGLFFYEGIIKQSLEVLNDGGT
HHEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCE
IAFEIGYDQKIQVSHILNEYGFKDVLCIKDLAGKDRVIKARKY
EEEEECCCCEEHHHHHHHHHCCCCEEHHHHCCCCCCEEECCCC
>Mature Secondary Structure
MILKDLLIEGYGVLKKASIGSYQIDAQLLLGKVLKKDRLFILTNPDYHIKEEEKEKYFQL
CCHHHHHHHCCCHHEECCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
IDLRKDKMPIKYILGTTEFMGLDFNIKKGVLIPRPDTEILVETVLEEIKNKNYKQICDVC
HHHHCCCCCEEEEECCHHHEECCEECCCCEEECCCCHHHHHHHHHHHHHCCCHHHHHHHH
CGSGIIGITIGYTLNNTEIICYDIEDIPYNTTKENILKHNLEDRVKVLKSDLLTEAIKEK
HCCCEEEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHH
RRFDLIVSNPPYIREDVIETLMDDVKKYEPFEALCGGKDGLFFYEGIIKQSLEVLNDGGT
HHEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCE
IAFEIGYDQKIQVSHILNEYGFKDVLCIKDLAGKDRVIKARKY
EEEEECCCCEEHHHHHHHHHCCCCEEHHHHCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]