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The map label for this gene is brnQ

Identifier: 170755372

GI number: 170755372

Start: 923517

End: 924833

Strand: Direct

Name: brnQ

Synonym: CLD_3758

Alternate gene names: 170755372

Gene position: 923517-924833 (Clockwise)

Preceding gene: 170756484

Following gene: 170756247

Centisome position: 23.33

GC content: 31.28

Gene sequence:

>1317_bases
ATGAAAAAAACATCATCAAAGGATTTCATAGTTATTGGGTTTGCACTTTTTGCAATGTTCTTTGGGGCAGGAAACTTAAT
ATTTCCACCTTTTATTGGAAATTTAGTAGGGGACAAAGCCCCAGTAGCCATTATTGGTTTTTTGATTACTGGAGTAGGTC
TACCATTGAGTGCTATAATAGCCTGTGCTAAAATCAATGGTACATTTTCAGATATATCAGGGAGAGTAGGAAAATACTTT
AGTGTTATATCTACAACAGCTTTGATATTAGCTATAGGACCTATGCTTTGTATACCAAGAACAGCAGCTACAACTTACGA
ATTAACAGTACAGCCATTATTTCCATCAATAGGTCCATTAGTATCAGCTATAATTTACTTTGCTATTTGTTTGGCTTTTG
TATTAAGACCATCAGGAATAGTTGACAGTATAGGTAAAGTTTTAACACCCTGTTTATTGCTTATGTTAGCAATTATAATA
ATAAAGGGATTAATTTCCCCACTAGGGCCTGTTGTTAGTACTAATTTTAAAGGAGTATTTTCAACATCTTTACTTGAAGG
ATATCAAACAATGGATACTATGGGTTCAGTAATATATGCATCAATAATTTTAGCATCTGTAAGAAGTAAGGGGTATACAG
ATGAAAAGGATGTAATAAGTGTTACTATAAAATCAGGCATAGTTGCTATAGCAGGATTGGGATTGGTTTATGGTGGACTT
ATGGTTTTAGGAAGTCAAACTTCACAAATAATTTCTGGTGAAGAAATAGGAAGGACTGCTTTAGTTATTGAAATAGTTAA
AAGAGATTTAGGTAATATAGGAATAGTCATATTAGGAATAGCTGTTGGACTTGCATGCCTTACTACAGCTATTGGACTTT
TATCTACAGGAGCAGAATACCTTAGTAAATTAACTAAAGGTAAAGTATCCTATAATGCTTTTGCAATTATTATAAGTGTG
GTAAGTGGAATACTTGGAATAGGTGGAGTTGATAAGATAATCAAATTTTCAGTTCCAATATTACAAATTTTATATCCAAT
AGTAATTGTATTAATAGTAATAACTTTATTAGGTAAAGTTGTTAAAAATGATAGTGTAGTAAAATTTACTGTTTATATCA
CATTAATAGTAAGTATGATAGATACAATGAATACATTAACAGGTGAGAAAGTAGCTTTTATAAAAAGTATTTTAGGAATC
ATACCTCTTTCTAGTGTTGGTTTTGCATGGCTAATTCCAGCAATTATAGCATTCATAATAGGGACAATAGCTTTTGGAAA
TAAACAAGAAAAAAATCCACAAGCAGCGGATTTTTAA

Upstream 100 bases:

>100_bases
TTCAGAATTTAATCAATTTTCCCAATAATCTTAATATAATATAATGACATACAATATAATGACTTAAAAAATTATATTGA
ATTAGGATTGAGGGGGTAAA

Downstream 100 bases:

>100_bases
TATAATAAAACTAAAAGTTGTCTCAAAATTAAATCTATTTTGAGACAACCCTTTTCACAAGATAAAAATATGAGGTATCT
AATTTTTTATTATATCTTCA

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MKKTSSKDFIVIGFALFAMFFGAGNLIFPPFIGNLVGDKAPVAIIGFLITGVGLPLSAIIACAKINGTFSDISGRVGKYF
SVISTTALILAIGPMLCIPRTAATTYELTVQPLFPSIGPLVSAIIYFAICLAFVLRPSGIVDSIGKVLTPCLLLMLAIII
IKGLISPLGPVVSTNFKGVFSTSLLEGYQTMDTMGSVIYASIILASVRSKGYTDEKDVISVTIKSGIVAIAGLGLVYGGL
MVLGSQTSQIISGEEIGRTALVIEIVKRDLGNIGIVILGIAVGLACLTTAIGLLSTGAEYLSKLTKGKVSYNAFAIIISV
VSGILGIGGVDKIIKFSVPILQILYPIVIVLIVITLLGKVVKNDSVVKFTVYITLIVSMIDTMNTLTGEKVAFIKSILGI
IPLSSVGFAWLIPAIIAFIIGTIAFGNKQEKNPQAADF

Sequences:

>Translated_438_residues
MKKTSSKDFIVIGFALFAMFFGAGNLIFPPFIGNLVGDKAPVAIIGFLITGVGLPLSAIIACAKINGTFSDISGRVGKYF
SVISTTALILAIGPMLCIPRTAATTYELTVQPLFPSIGPLVSAIIYFAICLAFVLRPSGIVDSIGKVLTPCLLLMLAIII
IKGLISPLGPVVSTNFKGVFSTSLLEGYQTMDTMGSVIYASIILASVRSKGYTDEKDVISVTIKSGIVAIAGLGLVYGGL
MVLGSQTSQIISGEEIGRTALVIEIVKRDLGNIGIVILGIAVGLACLTTAIGLLSTGAEYLSKLTKGKVSYNAFAIIISV
VSGILGIGGVDKIIKFSVPILQILYPIVIVLIVITLLGKVVKNDSVVKFTVYITLIVSMIDTMNTLTGEKVAFIKSILGI
IPLSSVGFAWLIPAIIAFIIGTIAFGNKQEKNPQAADF
>Mature_438_residues
MKKTSSKDFIVIGFALFAMFFGAGNLIFPPFIGNLVGDKAPVAIIGFLITGVGLPLSAIIACAKINGTFSDISGRVGKYF
SVISTTALILAIGPMLCIPRTAATTYELTVQPLFPSIGPLVSAIIYFAICLAFVLRPSGIVDSIGKVLTPCLLLMLAIII
IKGLISPLGPVVSTNFKGVFSTSLLEGYQTMDTMGSVIYASIILASVRSKGYTDEKDVISVTIKSGIVAIAGLGLVYGGL
MVLGSQTSQIISGEEIGRTALVIEIVKRDLGNIGIVILGIAVGLACLTTAIGLLSTGAEYLSKLTKGKVSYNAFAIIISV
VSGILGIGGVDKIIKFSVPILQILYPIVIVLIVITLLGKVVKNDSVVKFTVYITLIVSMIDTMNTLTGEKVAFIKSILGI
IPLSSVGFAWLIPAIIAFIIGTIAFGNKQEKNPQAADF

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=428, Percent_Identity=31.5420560747664, Blast_Score=218, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 45877; Mature: 45877

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTSSKDFIVIGFALFAMFFGAGNLIFPPFIGNLVGDKAPVAIIGFLITGVGLPLSAII
CCCCCCCCEEEHHHHHHHHHHCCCCEECCHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
ACAKINGTFSDISGRVGKYFSVISTTALILAIGPMLCIPRTAATTYELTVQPLFPSIGPL
HHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHH
VSAIIYFAICLAFVLRPSGIVDSIGKVLTPCLLLMLAIIIIKGLISPLGPVVSTNFKGVF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHH
STSLLEGYQTMDTMGSVIYASIILASVRSKGYTDEKDVISVTIKSGIVAIAGLGLVYGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEHHHHHHHHHHHHHHHHHH
MVLGSQTSQIISGEEIGRTALVIEIVKRDLGNIGIVILGIAVGLACLTTAIGLLSTGAEY
HHHCCCHHHHHCHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHH
LSKLTKGKVSYNAFAIIISVVSGILGIGGVDKIIKFSVPILQILYPIVIVLIVITLLGKV
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKNDSVVKFTVYITLIVSMIDTMNTLTGEKVAFIKSILGIIPLSSVGFAWLIPAIIAFII
HCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHCHHHHHHHHHHHHHH
GTIAFGNKQEKNPQAADF
HHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKTSSKDFIVIGFALFAMFFGAGNLIFPPFIGNLVGDKAPVAIIGFLITGVGLPLSAII
CCCCCCCCEEEHHHHHHHHHHCCCCEECCHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
ACAKINGTFSDISGRVGKYFSVISTTALILAIGPMLCIPRTAATTYELTVQPLFPSIGPL
HHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHH
VSAIIYFAICLAFVLRPSGIVDSIGKVLTPCLLLMLAIIIIKGLISPLGPVVSTNFKGVF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHH
STSLLEGYQTMDTMGSVIYASIILASVRSKGYTDEKDVISVTIKSGIVAIAGLGLVYGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEHHHHHHHHHHHHHHHHHH
MVLGSQTSQIISGEEIGRTALVIEIVKRDLGNIGIVILGIAVGLACLTTAIGLLSTGAEY
HHHCCCHHHHHCHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHH
LSKLTKGKVSYNAFAIIISVVSGILGIGGVDKIIKFSVPILQILYPIVIVLIVITLLGKV
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKNDSVVKFTVYITLIVSMIDTMNTLTGEKVAFIKSILGIIPLSSVGFAWLIPAIIAFII
HCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHCHHHHHHHHHHHHHH
GTIAFGNKQEKNPQAADF
HHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]