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The map label for this gene is brnQ

Identifier: 170756484

GI number: 170756484

Start: 922050

End: 923351

Strand: Direct

Name: brnQ

Synonym: CLD_3759

Alternate gene names: 170756484

Gene position: 922050-923351 (Clockwise)

Preceding gene: 170754721

Following gene: 170755372

Centisome position: 23.29

GC content: 32.1

Gene sequence:

>1302_bases
ATGAAGAAAACATCAGCAAAAGATTTCATAGTTATTGGTTTTGCACTTTTCGCAATGTTTTTTGGGGCAGGCAACTTAAT
TTTCCCACCTTTTATGGGGAAATTAGTAGGTGATAAGGCACCGGTAGCAATTATTGGTTTTTTAATTACTGGAGTAGGTT
TGCCGTTAAGTGGCATAATAGCCTGTGCTAAAATTAATGGAACATTCTCAGATATGTCGGAAAGAGTAGGTAAAAAATTT
TCTATTATAGCTACAACTGCATTGATATTAGCTATAGGACCGATGCTTGCAATACCAAGAACAGCAGCCACAACTTATGA
ATTGGGAATACATCCAATATTTCCATCTTTATCACCAGTTGTAGGAGTCGTAATTTATTTTGTTATTTGTTTAGCATTTG
TATTAAGGCCATCAGGAATAGTAGATAATATAGGTAAAATTTTAACACCAGCTTTATTGGTTTTGTTATCAATTATAATA
ATTAAAGGATTAGTTTCACCACTAGGACCAGTTATTAATACTAATTTTGAAGGAGCTTTTTCAAAATCTTTACTTGAAGG
TTATCAAACAATGGATGCTATGGCATCAGTAATATTTGCATCAATAATTTTAACAGCAGTAAAAGCTAAGGGATATACAG
ATGAAAATGATATAATATCTGCTACTATAAAATCAGGTGTTGTGGCTATAGTAGGATTAGCTTTTGTTTATGGCGGACTT
ATGATTTTAGGAAGTCAAACTTCACAAATAATTCCTGGAGAAATAGGAAGATCAGCTTTAGTTGTTGAGATAGTTAAGAT
GGACTTAGGAAATGTAGGTACAGTTATATTAGGATTAGCGGTTGGACTTGCATGTCTTACTACAGCTATCGGACTTATAT
CTACAGGAGCAGAGTACTTTAGTTCCTTAACTAATGGGAAAGTTTCTTATAATGCTTTTGCAATTATTATAAGTTCTATA
AGTGCTTTACTTGGAACAGGTGGAGTTGAAAAGATAGTTAAGTTTGCAGTACCAGTGTTACAGATTTTATATCCAATAGT
AATTGTATTAATAATAATAACTTTAGCAGGAAAACTTGTTAAAAATAATAATGTTGTAAAGATAACTGTTTATACTACGC
TAATAATAAGTGTAGTAGATGCAATCAATTCATTATCAGGTGGAAATGTAGGATTCTTAAAGGGATTATTAGGAATTATA
CCACTTTCTAACGTCGGTTTTTCATGGTTAGTTCCAGCAATTATAGCATTTATAGTAGGTACAGTAGCTTTTGGAAAGAA
ACAAGATATAGCAGATGCTTAA

Upstream 100 bases:

>100_bases
ATAAAATTAAAATTTCAGAATTCATTAAATTTTGAAAATAAAAATAATATGATATAATAACTAATAAAATTATATAAAAT
TTGAGTTAAGGGGGACTAAA

Downstream 100 bases:

>100_bases
TTTAATATAGTAAAAAGCACCTTGTTGTAAAACAATAAGGTGTTTTTTTGTTTTTTCATTAAAATTTCAGAATTTAATCA
ATTTTCCCAATAATCTTAAT

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKKTSAKDFIVIGFALFAMFFGAGNLIFPPFMGKLVGDKAPVAIIGFLITGVGLPLSGIIACAKINGTFSDMSERVGKKF
SIIATTALILAIGPMLAIPRTAATTYELGIHPIFPSLSPVVGVVIYFVICLAFVLRPSGIVDNIGKILTPALLVLLSIII
IKGLVSPLGPVINTNFEGAFSKSLLEGYQTMDAMASVIFASIILTAVKAKGYTDENDIISATIKSGVVAIVGLAFVYGGL
MILGSQTSQIIPGEIGRSALVVEIVKMDLGNVGTVILGLAVGLACLTTAIGLISTGAEYFSSLTNGKVSYNAFAIIISSI
SALLGTGGVEKIVKFAVPVLQILYPIVIVLIIITLAGKLVKNNNVVKITVYTTLIISVVDAINSLSGGNVGFLKGLLGII
PLSNVGFSWLVPAIIAFIVGTVAFGKKQDIADA

Sequences:

>Translated_433_residues
MKKTSAKDFIVIGFALFAMFFGAGNLIFPPFMGKLVGDKAPVAIIGFLITGVGLPLSGIIACAKINGTFSDMSERVGKKF
SIIATTALILAIGPMLAIPRTAATTYELGIHPIFPSLSPVVGVVIYFVICLAFVLRPSGIVDNIGKILTPALLVLLSIII
IKGLVSPLGPVINTNFEGAFSKSLLEGYQTMDAMASVIFASIILTAVKAKGYTDENDIISATIKSGVVAIVGLAFVYGGL
MILGSQTSQIIPGEIGRSALVVEIVKMDLGNVGTVILGLAVGLACLTTAIGLISTGAEYFSSLTNGKVSYNAFAIIISSI
SALLGTGGVEKIVKFAVPVLQILYPIVIVLIIITLAGKLVKNNNVVKITVYTTLIISVVDAINSLSGGNVGFLKGLLGII
PLSNVGFSWLVPAIIAFIVGTVAFGKKQDIADA
>Mature_433_residues
MKKTSAKDFIVIGFALFAMFFGAGNLIFPPFMGKLVGDKAPVAIIGFLITGVGLPLSGIIACAKINGTFSDMSERVGKKF
SIIATTALILAIGPMLAIPRTAATTYELGIHPIFPSLSPVVGVVIYFVICLAFVLRPSGIVDNIGKILTPALLVLLSIII
IKGLVSPLGPVINTNFEGAFSKSLLEGYQTMDAMASVIFASIILTAVKAKGYTDENDIISATIKSGVVAIVGLAFVYGGL
MILGSQTSQIIPGEIGRSALVVEIVKMDLGNVGTVILGLAVGLACLTTAIGLISTGAEYFSSLTNGKVSYNAFAIIISSI
SALLGTGGVEKIVKFAVPVLQILYPIVIVLIIITLAGKLVKNNNVVKITVYTTLIISVVDAINSLSGGNVGFLKGLLGII
PLSNVGFSWLVPAIIAFIVGTVAFGKKQDIADA

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=421, Percent_Identity=30.8788598574822, Blast_Score=219, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 44895; Mature: 44895

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTSAKDFIVIGFALFAMFFGAGNLIFPPFMGKLVGDKAPVAIIGFLITGVGLPLSGII
CCCCCCCHHHHHHHHHHHHHHCCCCEECCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHH
ACAKINGTFSDMSERVGKKFSIIATTALILAIGPMLAIPRTAATTYELGIHPIFPSLSPV
HHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHH
VGVVIYFVICLAFVLRPSGIVDNIGKILTPALLVLLSIIIIKGLVSPLGPVINTNFEGAF
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHH
SKSLLEGYQTMDAMASVIFASIILTAVKAKGYTDENDIISATIKSGVVAIVGLAFVYGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MILGSQTSQIIPGEIGRSALVVEIVKMDLGNVGTVILGLAVGLACLTTAIGLISTGAEYF
HEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
SSLTNGKVSYNAFAIIISSISALLGTGGVEKIVKFAVPVLQILYPIVIVLIIITLAGKLV
HHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNNNVVKITVYTTLIISVVDAINSLSGGNVGFLKGLLGIIPLSNVGFSWLVPAIIAFIVG
CCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TVAFGKKQDIADA
HHHCCCCCCCCCC
>Mature Secondary Structure
MKKTSAKDFIVIGFALFAMFFGAGNLIFPPFMGKLVGDKAPVAIIGFLITGVGLPLSGII
CCCCCCCHHHHHHHHHHHHHHCCCCEECCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHH
ACAKINGTFSDMSERVGKKFSIIATTALILAIGPMLAIPRTAATTYELGIHPIFPSLSPV
HHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHH
VGVVIYFVICLAFVLRPSGIVDNIGKILTPALLVLLSIIIIKGLVSPLGPVINTNFEGAF
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHH
SKSLLEGYQTMDAMASVIFASIILTAVKAKGYTDENDIISATIKSGVVAIVGLAFVYGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MILGSQTSQIIPGEIGRSALVVEIVKMDLGNVGTVILGLAVGLACLTTAIGLISTGAEYF
HEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
SSLTNGKVSYNAFAIIISSISALLGTGGVEKIVKFAVPVLQILYPIVIVLIIITLAGKLV
HHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNNNVVKITVYTTLIISVVDAINSLSGGNVGFLKGLLGIIPLSNVGFSWLVPAIIAFIVG
CCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TVAFGKKQDIADA
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]