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The map label for this gene is cbiK

Identifier: 170755304

GI number: 170755304

Start: 1064503

End: 1065285

Strand: Direct

Name: cbiK

Synonym: CLD_3608

Alternate gene names: 170755304

Gene position: 1064503-1065285 (Clockwise)

Preceding gene: 170757050

Following gene: 170756165

Centisome position: 26.89

GC content: 25.93

Gene sequence:

>783_bases
ATGAATAAAAACAAAAGAGCTATTTTAGTGGTGAGTTTTGGTACTAGTTATGAACAATCATTAAAAGACTGTATAGAAAG
CACAGAAAATTTTATAAAAGAAAGTTTTAAAGATTATGATATAAAAAGAGCTTTTACTTCTTACATGATAATAAATAAAA
TATTAAAAAGAGATAATATAAAGATAAATAATCCTATAGAAGCATTAGAAGAACTTAAGAAGGAAGGCTATGAAGAAGTT
ATAGTTCAACCTCTACATATAATGTTTGGAGAAGAGTATGAAAAAATAGAAAAAGCTGTTTTAGAATTTAAAAATGAATT
TAAGGTATTAAAATTAGGAAAGCCTTTATTATTTAAAAATGAAGACTACAAAATAGCAACAAAAGCTTTAAAGACTCAAC
TTCCTAAATTAAATAAAGAGGAAGCTATAATACTTATGGGACATGGAACAGAACATCCAGCTAATGCTGCTTATTTTCAA
TTTGATTATTATTTAAGAGAACACATAAATCCTAATATATATCTTTGTAGTGTAGAAAGTGATCCTTCTATAAAATCAAT
AATACCAAAATTAAAGAAATCTGGAGTAAAAAATGTTTTACTAATGCCTTTTATGTTAGTGGCTGGAGATCATGCTAACA
ATGATATGGCAGGAGATGATGAAGAATCCTGGAAAAATGTTTTGGAAAAGGAAGGCTTTAAAGTTGATTTATATTTAAAG
GGTCTAGGAGCAAATATAGAATTTCAAAAAATATATAGAGATCATATAAAAGAAGAAATCTAA

Upstream 100 bases:

>100_bases
ACAAAAAAATTATAAATTTAAATTCACGCATATATTTAATGAAATATTTAAAAAATATGTTAATTAAATAATATTATTAG
TATAGATCAGGAGGAGAAAC

Downstream 100 bases:

>100_bases
GGAGAGTATTTCATGGAAGTTAGAGCCAAATATCCAGGAAGTTTTGGAGAAATACTTCAAGGAAATTTAGGGGAAAAACC
TGTGTTGGTTTCTTCCCCTA

Product: anaerobic sirohydrochlorin cobaltochelatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNKNKRAILVVSFGTSYEQSLKDCIESTENFIKESFKDYDIKRAFTSYMIINKILKRDNIKINNPIEALEELKKEGYEEV
IVQPLHIMFGEEYEKIEKAVLEFKNEFKVLKLGKPLLFKNEDYKIATKALKTQLPKLNKEEAIILMGHGTEHPANAAYFQ
FDYYLREHINPNIYLCSVESDPSIKSIIPKLKKSGVKNVLLMPFMLVAGDHANNDMAGDDEESWKNVLEKEGFKVDLYLK
GLGANIEFQKIYRDHIKEEI

Sequences:

>Translated_260_residues
MNKNKRAILVVSFGTSYEQSLKDCIESTENFIKESFKDYDIKRAFTSYMIINKILKRDNIKINNPIEALEELKKEGYEEV
IVQPLHIMFGEEYEKIEKAVLEFKNEFKVLKLGKPLLFKNEDYKIATKALKTQLPKLNKEEAIILMGHGTEHPANAAYFQ
FDYYLREHINPNIYLCSVESDPSIKSIIPKLKKSGVKNVLLMPFMLVAGDHANNDMAGDDEESWKNVLEKEGFKVDLYLK
GLGANIEFQKIYRDHIKEEI
>Mature_260_residues
MNKNKRAILVVSFGTSYEQSLKDCIESTENFIKESFKDYDIKRAFTSYMIINKILKRDNIKINNPIEALEELKKEGYEEV
IVQPLHIMFGEEYEKIEKAVLEFKNEFKVLKLGKPLLFKNEDYKIATKALKTQLPKLNKEEAIILMGHGTEHPANAAYFQ
FDYYLREHINPNIYLCSVESDPSIKSIIPKLKKSGVKNVLLMPFMLVAGDHANNDMAGDDEESWKNVLEKEGFKVDLYLK
GLGANIEFQKIYRDHIKEEI

Specific function: Chelates cobalt to the cobalamin precursor as part of the anaerobic pathway to cobalamin biosynthesis [H]

COG id: COG4822

COG function: function code H; Cobalamin biosynthesis protein CbiK, Co2+ chelatase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CbiK family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010388 [H]

Pfam domain/function: PF06180 CbiK [H]

EC number: =4.99.1.3 [H]

Molecular weight: Translated: 30150; Mature: 30150

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNKRAILVVSFGTSYEQSLKDCIESTENFIKESFKDYDIKRAFTSYMIINKILKRDNI
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KINNPIEALEELKKEGYEEVIVQPLHIMFGEEYEKIEKAVLEFKNEFKVLKLGKPLLFKN
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCEEEECCCCEEEEC
EDYKIATKALKTQLPKLNKEEAIILMGHGTEHPANAAYFQFDYYLREHINPNIYLCSVES
CCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHCCCCCEEEEEECC
DPSIKSIIPKLKKSGVKNVLLMPFMLVAGDHANNDMAGDDEESWKNVLEKEGFKVDLYLK
CCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEE
GLGANIEFQKIYRDHIKEEI
ECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKNKRAILVVSFGTSYEQSLKDCIESTENFIKESFKDYDIKRAFTSYMIINKILKRDNI
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KINNPIEALEELKKEGYEEVIVQPLHIMFGEEYEKIEKAVLEFKNEFKVLKLGKPLLFKN
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCEEEECCCCEEEEC
EDYKIATKALKTQLPKLNKEEAIILMGHGTEHPANAAYFQFDYYLREHINPNIYLCSVES
CCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHCCCCCEEEEEECC
DPSIKSIIPKLKKSGVKNVLLMPFMLVAGDHANNDMAGDDEESWKNVLEKEGFKVDLYLK
CCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEE
GLGANIEFQKIYRDHIKEEI
ECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609; 9150215; 10451360 [H]