Click here to switch to the map view.

The map label for this gene is cobB [H]

Identifier: 170755003

GI number: 170755003

Start: 81393

End: 82136

Strand: Direct

Name: cobB [H]

Synonym: CLD_0739

Alternate gene names: 170755003

Gene position: 81393-82136 (Clockwise)

Preceding gene: 170757604

Following gene: 170756307

Centisome position: 2.06

GC content: 27.28

Gene sequence:

>744_bases
ATGAGGCTAGAAAAACTAAAGCATATTATAGATAATAGTGAAAATATAGTGTTTTTAGGTGGAGCGGGAGTGTCTACAGA
AAGTAATATTCCGGATTTTAGATCAAATAATGGATTATACAAATCAAAAAATAATTTAAATTATTCACCAGAGACAATAT
TAAGTCATAGTTTTTTTAAGAATAATACTAAAGAATTTTTTCAATTTTATAAAGATAGGATGATATTTAAAGATGCAAAG
CCTAATTTAGCCCATTATGCTTTAACAGAGTTAGAACAGATAGGAAAACTAAAGGCTATAATAACGCAAAATATTGATGG
ATTACATCAGTTATCAGGAGCTAAGAATGTATTAGAGTTACATGGAAGTGTGCATAGAAATTATTGTGTTAATTGTGGAG
AAAAATATAATTTAGATTATATATTAAATACTGAAAATAGTAGTGAAGATATACCGCATTGTAAAAAATGTGGAAGTATA
GTTAGACCAGATGTAGTGCTTTATGAAGAAGGATTAGACATGGATACTATAAGCAAAGCCATATATTATATACAAAATGC
AGATGTGCTTATAGTAGGTGGTACATCACTTGTAGTATATCCTGCTGCTGGATTAGTTAATTATTATAAGGGTAAAAAAT
TAGTTTTAATAAATAAAGCAGAAACATCTTATGACAAAAAAGCTGACTTAGTTATTCATGATTCTATAGGTAGTGTTTTA
GAAAAAGCAATTAAAAAAAACTAA

Upstream 100 bases:

>100_bases
TTTATAATACTTATTTCTTCTTTATGAGTAATTAAGTTTTAATAATTGTATTATTAGTATATAATAATTATGATTAATAA
CCTATTTAGGAGATGATAAT

Downstream 100 bases:

>100_bases
TCAAATTTTTGGTTAGTTTTTTAATTTTAATATTGACAAGTTTTAAATTTCTGATATAATAAATTATGTTGAATTGAATA
TGGCGCTATAGCCAAGTGGT

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MRLEKLKHIIDNSENIVFLGGAGVSTESNIPDFRSNNGLYKSKNNLNYSPETILSHSFFKNNTKEFFQFYKDRMIFKDAK
PNLAHYALTELEQIGKLKAIITQNIDGLHQLSGAKNVLELHGSVHRNYCVNCGEKYNLDYILNTENSSEDIPHCKKCGSI
VRPDVVLYEEGLDMDTISKAIYYIQNADVLIVGGTSLVVYPAAGLVNYYKGKKLVLINKAETSYDKKADLVIHDSIGSVL
EKAIKKN

Sequences:

>Translated_247_residues
MRLEKLKHIIDNSENIVFLGGAGVSTESNIPDFRSNNGLYKSKNNLNYSPETILSHSFFKNNTKEFFQFYKDRMIFKDAK
PNLAHYALTELEQIGKLKAIITQNIDGLHQLSGAKNVLELHGSVHRNYCVNCGEKYNLDYILNTENSSEDIPHCKKCGSI
VRPDVVLYEEGLDMDTISKAIYYIQNADVLIVGGTSLVVYPAAGLVNYYKGKKLVLINKAETSYDKKADLVIHDSIGSVL
EKAIKKN
>Mature_247_residues
MRLEKLKHIIDNSENIVFLGGAGVSTESNIPDFRSNNGLYKSKNNLNYSPETILSHSFFKNNTKEFFQFYKDRMIFKDAK
PNLAHYALTELEQIGKLKAIITQNIDGLHQLSGAKNVLELHGSVHRNYCVNCGEKYNLDYILNTENSSEDIPHCKKCGSI
VRPDVVLYEEGLDMDTISKAIYYIQNADVLIVGGTSLVVYPAAGLVNYYKGKKLVLINKAETSYDKKADLVIHDSIGSVL
EKAIKKN

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912662, Length=272, Percent_Identity=28.6764705882353, Blast_Score=124, Evalue=7e-29,
Organism=Homo sapiens, GI6912664, Length=267, Percent_Identity=28.8389513108614, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI13787215, Length=224, Percent_Identity=31.6964285714286, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI300795542, Length=254, Percent_Identity=29.1338582677165, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI6912660, Length=212, Percent_Identity=35.8490566037736, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI13775602, Length=217, Percent_Identity=35.4838709677419, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI13775600, Length=217, Percent_Identity=35.4838709677419, Blast_Score=117, Evalue=9e-27,
Organism=Homo sapiens, GI300797577, Length=256, Percent_Identity=29.296875, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI63054862, Length=205, Percent_Identity=36.5853658536585, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI300797597, Length=250, Percent_Identity=29.6, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI300797705, Length=194, Percent_Identity=35.5670103092784, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI7657575, Length=212, Percent_Identity=30.188679245283, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI215982798, Length=153, Percent_Identity=30.718954248366, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI308199517, Length=201, Percent_Identity=34.3283582089552, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17541892, Length=211, Percent_Identity=34.1232227488152, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71990482, Length=278, Percent_Identity=29.8561151079137, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17567771, Length=277, Percent_Identity=31.0469314079422, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71990487, Length=282, Percent_Identity=28.7234042553192, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17505510, Length=241, Percent_Identity=28.2157676348548, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325242, Length=257, Percent_Identity=31.5175097276265, Blast_Score=105, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324599, Length=309, Percent_Identity=28.4789644012945, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320163, Length=251, Percent_Identity=27.8884462151394, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324504, Length=252, Percent_Identity=28.1746031746032, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648389, Length=210, Percent_Identity=36.6666666666667, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI28571445, Length=274, Percent_Identity=28.4671532846715, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI17137536, Length=223, Percent_Identity=30.4932735426009, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24645650, Length=257, Percent_Identity=29.1828793774319, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI28571443, Length=195, Percent_Identity=28.7179487179487, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28571441, Length=195, Percent_Identity=28.7179487179487, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27756; Mature: 27756

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLEKLKHIIDNSENIVFLGGAGVSTESNIPDFRSNNGLYKSKNNLNYSPETILSHSFFK
CCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHC
NNTKEFFQFYKDRMIFKDAKPNLAHYALTELEQIGKLKAIITQNIDGLHQLSGAKNVLEL
CCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHH
HGSVHRNYCVNCGEKYNLDYILNTENSSEDIPHCKKCGSIVRPDVVLYEEGLDMDTISKA
HHHHCCCHHCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCEEEEECCCCHHHHHHH
IYYIQNADVLIVGGTSLVVYPAAGLVNYYKGKKLVLINKAETSYDKKADLVIHDSIGSVL
HHEEECCCEEEECCCEEEEECCHHHHHHHCCCEEEEEECCCCCCCCCCCEEEECHHHHHH
EKAIKKN
HHHHHCC
>Mature Secondary Structure
MRLEKLKHIIDNSENIVFLGGAGVSTESNIPDFRSNNGLYKSKNNLNYSPETILSHSFFK
CCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHC
NNTKEFFQFYKDRMIFKDAKPNLAHYALTELEQIGKLKAIITQNIDGLHQLSGAKNVLEL
CCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHH
HGSVHRNYCVNCGEKYNLDYILNTENSSEDIPHCKKCGSIVRPDVVLYEEGLDMDTISKA
HHHHCCCHHCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCEEEEECCCCHHHHHHH
IYYIQNADVLIVGGTSLVVYPAAGLVNYYKGKKLVLINKAETSYDKKADLVIHDSIGSVL
HHEEECCCEEEECCCEEEEECCHHHHHHHCCCEEEEEECCCCCCCCCCCEEEECHHHHHH
EKAIKKN
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]