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The map label for this gene is rimO [H]

Identifier: 170754856

GI number: 170754856

Start: 2522623

End: 2523960

Strand: Reverse

Name: rimO [H]

Synonym: CLD_2234

Alternate gene names: 170754856

Gene position: 2523960-2522623 (Counterclockwise)

Preceding gene: 170755708

Following gene: 170757752

Centisome position: 63.76

GC content: 28.33

Gene sequence:

>1338_bases
GTGGAAAAAATTAAAGTAGCATTAGTTAGCTTAGGTTGTGATAAAAATAGAATTGATTCAGAACTTATGCTATACAAATT
AAATGAAGAAGCAGAACTAGTAAAAAATCCTAAAGAAGCACAGGTAATTATAGTAAATACCTGTGGATTTATTGAAACCG
CTAAGGAAGAATCTATAAATACCATTTTACAAATGGCTTCCTATAAAAAAACTCATAATTGCAAAGTATTAGTAGTTACA
GGATGTTTGACTCAAAGATATAAAGGGGAACTTAAAGAACTTATCCCAGAAATGGATATTATGTTAGGGGTAAATGATTA
TGATAAGTTACTTGAAAGTATAAAAGTCTTTTTGAAATCAGGGGAAAAAAGTTTTTATCATAAATATAGTGATACAAAAA
TAAATGAGGGAAATAGAATATTAACTACGCCAACATACACAGCCTATGTGAGAATAGCAGAAGGGTGTAATAATTTTTGT
ACTTACTGTGCCATACCACGCATAAGAGGAAAGTATAGAAGTAGAAAAAAAGAAAATATATTAAAGGAAGTAGAAAATCT
AGCTAAACAAGGAGTTAAAGAAATAATATTGATAGCTCAAGATACAACCATGTATGGAATAGATATATATGGCAAAAAAG
TTTTACATGAGCTTCTAAGAGATATTTCTAAAGTTGAAGGTGTAAAGTGGATAAGATTATTATACTGTTACCCAGAGGAA
ATAACTAAGGAACTTATAGAAGAGATCAAAAATAATGATAAGGTATGTAAATATTTGGATTTGCCTATACAGCAGATAAG
TAATTCTGTTTTAAAAAGAATGGGAAGAAAAACTACAAAGGAAACTATCATAAATATAATAAAAAAATTAAGAAAAGAAA
TAGAAGGTATTACTTTAAGGACTTCATTAATAGTAGGATTTCCAGGGGAAACAGAAGGAGAGTTTAGTGAACTTAAAGAA
TTTGTTTCAGATGTAAAATTAGATAAATTAGGTGTATTTAAATATTCAAAGGAAGAAGGAACGTCAGCAGCTCTTATGGA
GGAACAAATAGATGAAGAAATAAAAGAAAAAAGAGAAGAAGAAATAATGATACTTCAGCAAAGTATATCTAAAGACATAA
ATAAAGAGAAAATAGGTAAAATATATGAAGTCATAGTAGAAGGCATCAAAGAAGATATGTATTATGGGAGAAATTATGAG
ATGTCACCGGAGATAGATGGAGAAATTTACTTTGAAAAAGATGAAAACGTAAGAATTGGTGATATAATAAAGGTGAAGGT
AACTCATAGTTTAGAATATGATTTAATAGGAGTTGTTTATAATGAACTTAGCAAATAA

Upstream 100 bases:

>100_bases
ATGTAAATACTTTTAAAATTCAAGTTACTTAATACTTGTAAAATTTCTTAAGTAGATTTAAAATGTTTCTTGTGACATAA
AAAATGTAGGAGGGGAACAG

Downstream 100 bases:

>100_bases
ACTAACTATATTAAGAATTTTTTTAGTCCCAATATTTCTTATATTTATATCAGTAAAAAACATACCCTATGGTACAGTAA
TAGCTACTGCAGTTTTTGTA

Product: RNA modification protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MEKIKVALVSLGCDKNRIDSELMLYKLNEEAELVKNPKEAQVIIVNTCGFIETAKEESINTILQMASYKKTHNCKVLVVT
GCLTQRYKGELKELIPEMDIMLGVNDYDKLLESIKVFLKSGEKSFYHKYSDTKINEGNRILTTPTYTAYVRIAEGCNNFC
TYCAIPRIRGKYRSRKKENILKEVENLAKQGVKEIILIAQDTTMYGIDIYGKKVLHELLRDISKVEGVKWIRLLYCYPEE
ITKELIEEIKNNDKVCKYLDLPIQQISNSVLKRMGRKTTKETIINIIKKLRKEIEGITLRTSLIVGFPGETEGEFSELKE
FVSDVKLDKLGVFKYSKEEGTSAALMEEQIDEEIKEKREEEIMILQQSISKDINKEKIGKIYEVIVEGIKEDMYYGRNYE
MSPEIDGEIYFEKDENVRIGDIIKVKVTHSLEYDLIGVVYNELSK

Sequences:

>Translated_445_residues
MEKIKVALVSLGCDKNRIDSELMLYKLNEEAELVKNPKEAQVIIVNTCGFIETAKEESINTILQMASYKKTHNCKVLVVT
GCLTQRYKGELKELIPEMDIMLGVNDYDKLLESIKVFLKSGEKSFYHKYSDTKINEGNRILTTPTYTAYVRIAEGCNNFC
TYCAIPRIRGKYRSRKKENILKEVENLAKQGVKEIILIAQDTTMYGIDIYGKKVLHELLRDISKVEGVKWIRLLYCYPEE
ITKELIEEIKNNDKVCKYLDLPIQQISNSVLKRMGRKTTKETIINIIKKLRKEIEGITLRTSLIVGFPGETEGEFSELKE
FVSDVKLDKLGVFKYSKEEGTSAALMEEQIDEEIKEKREEEIMILQQSISKDINKEKIGKIYEVIVEGIKEDMYYGRNYE
MSPEIDGEIYFEKDENVRIGDIIKVKVTHSLEYDLIGVVYNELSK
>Mature_445_residues
MEKIKVALVSLGCDKNRIDSELMLYKLNEEAELVKNPKEAQVIIVNTCGFIETAKEESINTILQMASYKKTHNCKVLVVT
GCLTQRYKGELKELIPEMDIMLGVNDYDKLLESIKVFLKSGEKSFYHKYSDTKINEGNRILTTPTYTAYVRIAEGCNNFC
TYCAIPRIRGKYRSRKKENILKEVENLAKQGVKEIILIAQDTTMYGIDIYGKKVLHELLRDISKVEGVKWIRLLYCYPEE
ITKELIEEIKNNDKVCKYLDLPIQQISNSVLKRMGRKTTKETIINIIKKLRKEIEGITLRTSLIVGFPGETEGEFSELKE
FVSDVKLDKLGVFKYSKEEGTSAALMEEQIDEEIKEKREEEIMILQQSISKDINKEKIGKIYEVIVEGIKEDMYYGRNYE
MSPEIDGEIYFEKDENVRIGDIIKVKVTHSLEYDLIGVVYNELSK

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=480, Percent_Identity=28.3333333333333, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI28872782, Length=480, Percent_Identity=28.3333333333333, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI93277076, Length=406, Percent_Identity=27.0935960591133, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1787057, Length=444, Percent_Identity=37.8378378378378, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1786882, Length=425, Percent_Identity=30.1176470588235, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17553146, Length=416, Percent_Identity=24.7596153846154, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71996771, Length=370, Percent_Identity=27.2972972972973, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI21356207, Length=470, Percent_Identity=26.8085106382979, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI19922432, Length=454, Percent_Identity=25.9911894273128, Blast_Score=138, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 51402; Mature: 51402

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIKVALVSLGCDKNRIDSELMLYKLNEEAELVKNPKEAQVIIVNTCGFIETAKEESIN
CCCCEEEEEEECCCCCCCCCEEEEEEECCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
TILQMASYKKTHNCKVLVVTGCLTQRYKGELKELIPEMDIMLGVNDYDKLLESIKVFLKS
HHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHC
GEKSFYHKYSDTKINEGNRILTTPTYTAYVRIAEGCNNFCTYCAIPRIRGKYRSRKKENI
CCHHHHHCCCCCEECCCCEEEECCCCEEEEEEHHCCCHHHHHHCCHHHHHHHHHHHHHHH
LKEVENLAKQGVKEIILIAQDTTMYGIDIYGKKVLHELLRDISKVEGVKWIRLLYCYPEE
HHHHHHHHHHCHHHEEEEEECCEEEEEEHHHHHHHHHHHHHHHHHCCHHEEHHEEECCHH
ITKELIEEIKNNDKVCKYLDLPIQQISNSVLKRMGRKTTKETIINIIKKLRKEIEGITLR
HHHHHHHHHHCCCCEEHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEE
TSLIVGFPGETEGEFSELKEFVSDVKLDKLGVFKYSKEEGTSAALMEEQIDEEIKEKREE
EEEEEECCCCCCCCHHHHHHHHHHCCCCHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
EIMILQQSISKDINKEKIGKIYEVIVEGIKEDMYYGRNYEMSPEIDGEIYFEKDENVRIG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEC
DIIKVKVTHSLEYDLIGVVYNELSK
CEEEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MEKIKVALVSLGCDKNRIDSELMLYKLNEEAELVKNPKEAQVIIVNTCGFIETAKEESIN
CCCCEEEEEEECCCCCCCCCEEEEEEECCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
TILQMASYKKTHNCKVLVVTGCLTQRYKGELKELIPEMDIMLGVNDYDKLLESIKVFLKS
HHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHC
GEKSFYHKYSDTKINEGNRILTTPTYTAYVRIAEGCNNFCTYCAIPRIRGKYRSRKKENI
CCHHHHHCCCCCEECCCCEEEECCCCEEEEEEHHCCCHHHHHHCCHHHHHHHHHHHHHHH
LKEVENLAKQGVKEIILIAQDTTMYGIDIYGKKVLHELLRDISKVEGVKWIRLLYCYPEE
HHHHHHHHHHCHHHEEEEEECCEEEEEEHHHHHHHHHHHHHHHHHCCHHEEHHEEECCHH
ITKELIEEIKNNDKVCKYLDLPIQQISNSVLKRMGRKTTKETIINIIKKLRKEIEGITLR
HHHHHHHHHHCCCCEEHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEE
TSLIVGFPGETEGEFSELKEFVSDVKLDKLGVFKYSKEEGTSAALMEEQIDEEIKEKREE
EEEEEECCCCCCCCHHHHHHHHHHCCCCHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
EIMILQQSISKDINKEKIGKIYEVIVEGIKEDMYYGRNYEMSPEIDGEIYFEKDENVRIG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEC
DIIKVKVTHSLEYDLIGVVYNELSK
CEEEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA