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The map label for this gene is nfsA [C]

Identifier: 170754826

GI number: 170754826

Start: 2189639

End: 2190139

Strand: Reverse

Name: nfsA [C]

Synonym: CLD_2586

Alternate gene names: 170754826

Gene position: 2190139-2189639 (Counterclockwise)

Preceding gene: 170755302

Following gene: 170757838

Centisome position: 55.33

GC content: 31.54

Gene sequence:

>501_bases
ATGAATGCTATATTAAAGAGAAGAAGTATAAGAAAATACAAGGATAAAAAAATAAGTGATGATATTGTTGAAGAATTATT
AAGAGCAGGAATGGCTGCACCTTCTGCTGTAAATGAACAACCTTGGCAATTTATAGTCTTAAGAGATAAAGAAACTATGA
AAAAGATAACTAAGGTGCATGAGTATTCAAAGATGTTATTAGAGGCAAACCTTGCCATTGTAGTTTGTGGAGATAAATCA
AAAGAATTAGTAGATGATTTTTGGGTACAAGATTGTTCAGCAGCTACAGAAAACATATTAATAGAGGCACAAGATAAAGG
ATTAGGTGCAGTATGGTTAGGTGTTTATCCAATAAAAGAGAGAGTAGATGGAATAAAAGAAATTCTAAATTTACCAGAAG
GTATAACTCCTTTATCTGTAATACCTATAGGATATCCAGATGAAGATAAGGAGCCGGTAGATAGATTTAGTAAAGAAAGA
GTACATTATGATAGATGGTAA

Upstream 100 bases:

>100_bases
GTAAGCGTAAAATTAAGCCTTCATTTTAATTCATTAATTTCTTTTAATTCACATAACAATAGTATAAAGTATTTAAAATA
ACAATTAGGGGGAATTATAA

Downstream 100 bases:

>100_bases
TTTATAAAATGTAAATATTGTAGCTAGATTTTAAATTGTAAACAGGTATTAAAGTGCGTTTTATGTTTTCATAAGCACAC
TTTTTATTAAATAAAATATA

Product: nitroreductase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MNAILKRRSIRKYKDKKISDDIVEELLRAGMAAPSAVNEQPWQFIVLRDKETMKKITKVHEYSKMLLEANLAIVVCGDKS
KELVDDFWVQDCSAATENILIEAQDKGLGAVWLGVYPIKERVDGIKEILNLPEGITPLSVIPIGYPDEDKEPVDRFSKER
VHYDRW

Sequences:

>Translated_166_residues
MNAILKRRSIRKYKDKKISDDIVEELLRAGMAAPSAVNEQPWQFIVLRDKETMKKITKVHEYSKMLLEANLAIVVCGDKS
KELVDDFWVQDCSAATENILIEAQDKGLGAVWLGVYPIKERVDGIKEILNLPEGITPLSVIPIGYPDEDKEPVDRFSKER
VHYDRW
>Mature_166_residues
MNAILKRRSIRKYKDKKISDDIVEELLRAGMAAPSAVNEQPWQFIVLRDKETMKKITKVHEYSKMLLEANLAIVVCGDKS
KELVDDFWVQDCSAATENILIEAQDKGLGAVWLGVYPIKERVDGIKEILNLPEGITPLSVIPIGYPDEDKEPVDRFSKER
VHYDRW

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 19027; Mature: 19027

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAILKRRSIRKYKDKKISDDIVEELLRAGMAAPSAVNEQPWQFIVLRDKETMKKITKVH
CCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHH
EYSKMLLEANLAIVVCGDKSKELVDDFWVQDCSAATENILIEAQDKGLGAVWLGVYPIKE
HHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEEECHHH
RVDGIKEILNLPEGITPLSVIPIGYPDEDKEPVDRFSKERVHYDRW
HHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNAILKRRSIRKYKDKKISDDIVEELLRAGMAAPSAVNEQPWQFIVLRDKETMKKITKVH
CCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHH
EYSKMLLEANLAIVVCGDKSKELVDDFWVQDCSAATENILIEAQDKGLGAVWLGVYPIKE
HHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEEECHHH
RVDGIKEILNLPEGITPLSVIPIGYPDEDKEPVDRFSKERVHYDRW
HHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]