| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is spoIVB
Identifier: 170754568
GI number: 170754568
Start: 1999760
End: 2000986
Strand: Reverse
Name: spoIVB
Synonym: CLD_2764
Alternate gene names: 170754568
Gene position: 2000986-1999760 (Counterclockwise)
Preceding gene: 170757077
Following gene: 170755469
Centisome position: 50.55
GC content: 29.18
Gene sequence:
>1227_bases ATGGACAGAAATAAGAAAAAATTTTTAAGCTTTTTTTTAATTCCTATCCTGTTAATAAGTTTGTTTACTTATTATAAGAT TCATCACATACCTACTACAATTTTTGTAAGAGAGGGAGAAAGAGTAAAAAACAATAGTTTTATTAAATTAACAGAGGATA TAAGTGCGGATTGCACAGTTTCTGATATAAAATCAAACAATAAAAAAATGAATGTCAAAATTTTAGGAATGGTTCCTGTA AAATCTGTTTCATTAAAACCCGTTTCTAAAGATATTATGTTGTACCCTGGTGGAAAACCTGTTGGAGTAAAGTTAAATAC AAAGGGAGTTTTAGTAATAGCTCTTTCAGATATAGAAACAGAAGGCGGAAAAGTACAAAGTCCCGCAGCTGTATCTGGTA TACAAATTGGAGATAGTATAATAAGTATAAATGGAAAAGAAATAACCAATTCAGAAGATGTAGAAAAGGAAATTAGAAAT TGTGAAGGAAAAGACTTAAAAATAGTAGCTTATAGAAAAGGTGAAAAAATAACAAAAAATATTAAGCCTGAAAAAGGTAA GAAAGATAATAATTATAAAATAGGATTATGGGTAAGAGATTCCACAGCTGGTGTAGGAACACTGACTTTTTATCATGATA AAAGTAAAAAATTTGCAGCCTTAGGTCATCCTATTACTGATGTAGACACAGGTACTATACTTACTATAGATTCAGGAGAA ATAGTACCGTCTTCTATAGTATCTATAAAAAAAGGTATAAAAGGTAATCCTGGAGAACTAAAAGGAATATTTTTAGATGA AGATTTGGTCTTAGGCAATATAGAAAATAATACAGAGTGTGGTATATTCGGAACATCAAATGTGAAATTAAGTAATAAAA AATATAAAAAACCTATGAAAGTAGCTTTAAGAAATGAAATAAAAGAAGGACCAGCTCAAATATTGACTACATTAGAAGGT GAAGAACCTAGTTTATACAATATAGAAATACAGAAGCTTTTATCTCAGGATAAACCAGGACCTAAGAGCATGATTATAAA AGTTACAGATCAAAATCTTATTAAAAAGACTGGAGGTATTGTTCAAGGCATGAGCGGAAGTCCTATAATACAAAATGATA AAATAGTTGGAGCTGTTACTCATGTTTTAATTAATAAGCCAGATGTAGGATATGGCATATATATAGAATGGATGTTAAAG GATGCAGGCATATTAGATAATAATTAA
Upstream 100 bases:
>100_bases TACAAAGAATTGGATTTTAAAGAAAATATACTACAGCATAATTATATTTGAAAGGGGAAATTTAAGAATAAATAAGATTT TAAGACAGGAGGTAAAAGTT
Downstream 100 bases:
>100_bases TTATTATAGTTAATTTTTTTATATAGATCTAAAGATTAATCTAAAAGCCACTAAGTAAGATTTAATATAAGTAGCATGAA TATAATAAATTAGTAATTTA
Product: stage IV sporulation protein B
Products: NA
Alternate protein names: Sporulation factor IV B protease; Stage IV sporulation protein B [H]
Number of amino acids: Translated: 408; Mature: 408
Protein sequence:
>408_residues MDRNKKKFLSFFLIPILLISLFTYYKIHHIPTTIFVREGERVKNNSFIKLTEDISADCTVSDIKSNNKKMNVKILGMVPV KSVSLKPVSKDIMLYPGGKPVGVKLNTKGVLVIALSDIETEGGKVQSPAAVSGIQIGDSIISINGKEITNSEDVEKEIRN CEGKDLKIVAYRKGEKITKNIKPEKGKKDNNYKIGLWVRDSTAGVGTLTFYHDKSKKFAALGHPITDVDTGTILTIDSGE IVPSSIVSIKKGIKGNPGELKGIFLDEDLVLGNIENNTECGIFGTSNVKLSNKKYKKPMKVALRNEIKEGPAQILTTLEG EEPSLYNIEIQKLLSQDKPGPKSMIIKVTDQNLIKKTGGIVQGMSGSPIIQNDKIVGAVTHVLINKPDVGYGIYIEWMLK DAGILDNN
Sequences:
>Translated_408_residues MDRNKKKFLSFFLIPILLISLFTYYKIHHIPTTIFVREGERVKNNSFIKLTEDISADCTVSDIKSNNKKMNVKILGMVPV KSVSLKPVSKDIMLYPGGKPVGVKLNTKGVLVIALSDIETEGGKVQSPAAVSGIQIGDSIISINGKEITNSEDVEKEIRN CEGKDLKIVAYRKGEKITKNIKPEKGKKDNNYKIGLWVRDSTAGVGTLTFYHDKSKKFAALGHPITDVDTGTILTIDSGE IVPSSIVSIKKGIKGNPGELKGIFLDEDLVLGNIENNTECGIFGTSNVKLSNKKYKKPMKVALRNEIKEGPAQILTTLEG EEPSLYNIEIQKLLSQDKPGPKSMIIKVTDQNLIKKTGGIVQGMSGSPIIQNDKIVGAVTHVLINKPDVGYGIYIEWMLK DAGILDNN >Mature_408_residues MDRNKKKFLSFFLIPILLISLFTYYKIHHIPTTIFVREGERVKNNSFIKLTEDISADCTVSDIKSNNKKMNVKILGMVPV KSVSLKPVSKDIMLYPGGKPVGVKLNTKGVLVIALSDIETEGGKVQSPAAVSGIQIGDSIISINGKEITNSEDVEKEIRN CEGKDLKIVAYRKGEKITKNIKPEKGKKDNNYKIGLWVRDSTAGVGTLTFYHDKSKKFAALGHPITDVDTGTILTIDSGE IVPSSIVSIKKGIKGNPGELKGIFLDEDLVLGNIENNTECGIFGTSNVKLSNKKYKKPMKVALRNEIKEGPAQILTTLEG EEPSLYNIEIQKLLSQDKPGPKSMIIKVTDQNLIKKTGGIVQGMSGSPIIQNDKIVGAVTHVLINKPDVGYGIYIEWMLK DAGILDNN
Specific function: Plays a central role in the sigma-K checkpoint which coordinates gene expression during the later stages of spore formation. The protease is activated by trans cleavage of the zymogen precursor producing SpoIVB-45 kDa. This undergoes further trimming by c
COG id: COG0750
COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1
Gene ontology:
Cell location: Forespore intermembrane space [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 peptidase S55 domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001478 - InterPro: IPR014219 - InterPro: IPR008763 [H]
Pfam domain/function: PF00595 PDZ; PF05580 Peptidase_S55 [H]
EC number: =3.4.21.116 [H]
Molecular weight: Translated: 44708; Mature: 44708
Theoretical pI: Translated: 9.81; Mature: 9.81
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MDRNKKKFLSFFLIPILLISLFTYYKIHHIPTTIFVREGERVKNNSFIKLTEDISADCTV CCCCHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCCCCCCCCEEEEECCCCCCCEE SDIKSNNKKMNVKILGMVPVKSVSLKPVSKDIMLYPGGKPVGVKLNTKGVLVIALSDIET EHHCCCCCEEEEEEEEEECCCCEECCCCCCCEEEECCCCEEEEEECCCCEEEEEEECCCC EGGKVQSPAAVSGIQIGDSIISINGKEITNSEDVEKEIRNCEGKDLKIVAYRKGEKITKN CCCEECCCCCCCCEEECCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCHHHCC IKPEKGKKDNNYKIGLWVRDSTAGVGTLTFYHDKSKKFAALGHPITDVDTGTILTIDSGE CCCCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCEEECCCCCCCCCCCEEEEECCCC IVPSSIVSIKKGIKGNPGELKGIFLDEDLVLGNIENNTECGIFGTSNVKLSNKKYKKPMK CCHHHHHHHHHCCCCCCCCEEEEEECCCEEEECCCCCCEEEEEECCCEEECCHHHCCHHH VALRNEIKEGPAQILTTLEGEEPSLYNIEIQKLLSQDKPGPKSMIIKVTDQNLIKKTGGI HHHHHHHCCCHHHHHEEECCCCCCEEEEEHHHHHHCCCCCCCEEEEEECCCHHHHHCCCE VQGMSGSPIIQNDKIVGAVTHVLINKPDVGYGIYIEWMLKDAGILDNN EECCCCCCEEECCCCCEEEEEEEECCCCCCEEEEEEEEEECCCCCCCC >Mature Secondary Structure MDRNKKKFLSFFLIPILLISLFTYYKIHHIPTTIFVREGERVKNNSFIKLTEDISADCTV CCCCHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCCCCCCCCEEEEECCCCCCCEE SDIKSNNKKMNVKILGMVPVKSVSLKPVSKDIMLYPGGKPVGVKLNTKGVLVIALSDIET EHHCCCCCEEEEEEEEEECCCCEECCCCCCCEEEECCCCEEEEEECCCCEEEEEEECCCC EGGKVQSPAAVSGIQIGDSIISINGKEITNSEDVEKEIRNCEGKDLKIVAYRKGEKITKN CCCEECCCCCCCCEEECCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCHHHCC IKPEKGKKDNNYKIGLWVRDSTAGVGTLTFYHDKSKKFAALGHPITDVDTGTILTIDSGE CCCCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCEEECCCCCCCCCCCEEEEECCCC IVPSSIVSIKKGIKGNPGELKGIFLDEDLVLGNIENNTECGIFGTSNVKLSNKKYKKPMK CCHHHHHHHHHCCCCCCCCEEEEEECCCEEEECCCCCCEEEEEECCCEEECCHHHCCHHH VALRNEIKEGPAQILTTLEGEEPSLYNIEIQKLLSQDKPGPKSMIIKVTDQNLIKKTGGI HHHHHHHCCCHHHHHEEECCCCCCEEEEEHHHHHHCCCCCCCEEEEEECCCHHHHHCCCE VQGMSGSPIIQNDKIVGAVTHVLINKPDVGYGIYIEWMLKDAGILDNN EECCCCCCEEECCCCCEEEEEEEECCCCCCEEEEEEEEEECCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 2106508; 8969508; 9384377; 11741860 [H]