Click here to switch to the map view.

The map label for this gene is spo0A

Identifier: 170755469

GI number: 170755469

Start: 1998509

End: 1999330

Strand: Reverse

Name: spo0A

Synonym: CLD_2765

Alternate gene names: 170755469

Gene position: 1999330-1998509 (Counterclockwise)

Preceding gene: 170754568

Following gene: 170757167

Centisome position: 50.51

GC content: 31.87

Gene sequence:

>822_bases
ATGGAAGAAACAAAGATCAATGTTATCATCGCAGATGATAATAAGGAATTTTGTAACATATTGAGTGATTATTTACTAAA
TCAAAGAGACATAATGGTTACAGGTATAGCTAATGATGGAGTAGAGGCTCTTAAATTGGTGGAAGAAAAAAAGCCAGATT
TAATAATACTAGATATAATAATGCCTCACTTAGATGGATTAGGGGTTTTGGAAAAGTTAAATAGCATAGATATTACTCCT
ATGCCAAGAGTAATTGTTTTGTCTGCTGTAGGACAGGATAAGATTACTCAAAGGGCTATAAACTTAGGTGCAGATTATTA
TGTAGTTAAACCTTTTGATATGGATGTTTTTACTAAAAGAATAAGACAAATGTTTAACAATACCATAAGTAGTGAAGAAG
TTAGAAGACCAGTAGTAATGCCGGAAACCTGTTCACAAGAAAAGATATCATTATCTAAAAATGAACCAGCAGATTTAGAG
CAAGAAATAACAGATATTATACATGAAATAGGCGTGCCAGCTCACATAAAAGGATATATGTATTTGAGAGAAGCTATAAC
TATGGTGGTTAATGACATGGAACTTTTATCTGCAGTAACAAAAGAATTATACCCTTCCATAGCTAAAAAATATAATACTA
CAGCAAGCAGAGTGGAAAGAGCTATAAGACACGCTATAGAAGTAGCTTGGTCAAGAGGTCAAGTTGAGACTATAAACAAT
ATATTTGGGTATACTATAAATAATGGAAAGGGAAAACCAACAAATTCTGAGTTTATTGCAATGGTGGCTGATAAATTAAG
ACTAAAAAATAGGGTTTGTTAA

Upstream 100 bases:

>100_bases
TTTATATATTTTTTCTCAAATAAGGAGGAATTGAATTTCTTTTGTCGAATAAATAATTTGTTAAGATATGGTAATTTTAT
CAAAAAGGAGAGTAGTTTAT

Downstream 100 bases:

>100_bases
ATATCTTATATTGATAGACTTTGTAAAAATCGGTATGGATTTTATTGTTTTATATCAATAGATTTTAAATTAAATTGTAT
AAGAATACCTTTTGTACATT

Product: sporulation transcription factor Spo0A

Products: protein L-glutamate+ methanol; methanol; Tapglu; methanol; Targlu; Trgglu; Tsrglu; NH3; protein-L-glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MEETKINVIIADDNKEFCNILSDYLLNQRDIMVTGIANDGVEALKLVEEKKPDLIILDIIMPHLDGLGVLEKLNSIDITP
MPRVIVLSAVGQDKITQRAINLGADYYVVKPFDMDVFTKRIRQMFNNTISSEEVRRPVVMPETCSQEKISLSKNEPADLE
QEITDIIHEIGVPAHIKGYMYLREAITMVVNDMELLSAVTKELYPSIAKKYNTTASRVERAIRHAIEVAWSRGQVETINN
IFGYTINNGKGKPTNSEFIAMVADKLRLKNRVC

Sequences:

>Translated_273_residues
MEETKINVIIADDNKEFCNILSDYLLNQRDIMVTGIANDGVEALKLVEEKKPDLIILDIIMPHLDGLGVLEKLNSIDITP
MPRVIVLSAVGQDKITQRAINLGADYYVVKPFDMDVFTKRIRQMFNNTISSEEVRRPVVMPETCSQEKISLSKNEPADLE
QEITDIIHEIGVPAHIKGYMYLREAITMVVNDMELLSAVTKELYPSIAKKYNTTASRVERAIRHAIEVAWSRGQVETINN
IFGYTINNGKGKPTNSEFIAMVADKLRLKNRVC
>Mature_273_residues
MEETKINVIIADDNKEFCNILSDYLLNQRDIMVTGIANDGVEALKLVEEKKPDLIILDIIMPHLDGLGVLEKLNSIDITP
MPRVIVLSAVGQDKITQRAINLGADYYVVKPFDMDVFTKRIRQMFNNTISSEEVRRPVVMPETCSQEKISLSKNEPADLE
QEITDIIHEIGVPAHIKGYMYLREAITMVVNDMELLSAVTKELYPSIAKKYNTTASRVERAIRHAIEVAWSRGQVETINN
IFGYTINNGKGKPTNSEFIAMVADKLRLKNRVC

Specific function: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the ex

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=109, Percent_Identity=36.697247706422, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=35.9649122807018, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR014879
- InterPro:   IPR012052
- InterPro:   IPR011991
- ProDom:   PD010908 [H]

Pfam domain/function: PF00072 Response_reg; PF08769 Spo0A_C [H]

EC number: 3.1.1.61 [C]

Molecular weight: Translated: 30838; Mature: 30838

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEETKINVIIADDNKEFCNILSDYLLNQRDIMVTGIANDGVEALKLVEEKKPDLIILDII
CCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEH
MPHLDGLGVLEKLNSIDITPMPRVIVLSAVGQDKITQRAINLGADYYVVKPFDMDVFTKR
HHCCCCHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEECCCHHHHHHH
IRQMFNNTISSEEVRRPVVMPETCSQEKISLSKNEPADLEQEITDIIHEIGVPAHIKGYM
HHHHHCCCCCHHHHCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
YLREAITMVVNDMELLSAVTKELYPSIAKKYNTTASRVERAIRHAIEVAWSRGQVETINN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
IFGYTINNGKGKPTNSEFIAMVADKLRLKNRVC
HHCEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEETKINVIIADDNKEFCNILSDYLLNQRDIMVTGIANDGVEALKLVEEKKPDLIILDII
CCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEH
MPHLDGLGVLEKLNSIDITPMPRVIVLSAVGQDKITQRAINLGADYYVVKPFDMDVFTKR
HHCCCCHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEECCCHHHHHHH
IRQMFNNTISSEEVRRPVVMPETCSQEKISLSKNEPADLEQEITDIIHEIGVPAHIKGYM
HHHHHCCCCCHHHHCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
YLREAITMVVNDMELLSAVTKELYPSIAKKYNTTASRVERAIRHAIEVAWSRGQVETINN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
IFGYTINNGKGKPTNSEFIAMVADKLRLKNRVC
HHCEEEECCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein L-glutamate Omethyl ester; Tapglu-Me; H2O; Targlu-Me; Trgglu-Me; Tsrglu-Me; Tsrgln; Trggln; Targln; Tapgln; protein-L-glutamine [C]

Specific reaction: Protein L-glutamate Omethyl ester + H2O = protein L-glutamate+ methanol H2O + Tapglu-Me = methanol + Tapglu H2O + Targlu-Me = methanol + Targlu H2O + Trgglu-Me = methanol + Trgglu H2O + Tsrglu-Me = methanol + Tsrglu H2O + Tsrgln = NH3 + Tsrglu H2O + Trggl

General reaction: Hydrolysis of carboxylic ester [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]