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The map label for this gene is maeB [C]

Identifier: 170754503

GI number: 170754503

Start: 307908

End: 309080

Strand: Direct

Name: maeB [C]

Synonym: CLD_0518

Alternate gene names: 170754503

Gene position: 307908-309080 (Clockwise)

Preceding gene: 170756684

Following gene: 170757488

Centisome position: 7.78

GC content: 33.59

Gene sequence:

>1173_bases
ATGAATTATTTTGAAGAAAGCTTAAAACTACATGAAGATAATAGAGGAAAAATAGAAATAAAATCAAAGATGGAAGTTAA
AACAAGAGATGATTTAAGTTTGGCTTATACGCCTGGAGTTGCAGAGCCATGTAGAAAGATTCATGAAAACGAAGAAAATG
TTTATAAATATACTTCAAAAGGAAATATGGTTGCAGTAGTTACAGATGGAACGGCAGTATTAGGATTAGGAGATATAGGG
CCTAAGGCTGGATTACCAGTTATGGAAGGGAAAGCTCTTTTGTTTAAAGAGTTTGCAAATGTTGATGCATTTCCAATATG
CTTAGATACAAAAGATGTTGATGAAATAGTAAAAACTGTAAAACTTATAGCTCCAGGATTTGGCGGAATTAATTTAGAAG
ATATTGGAGCTCCAAGATGCTTTGAAATAGAAGAGAAGCTAAAGAAAGAACTTGATATACCTGTATTTCATGATGATCAA
CATGGAACTGCAATAGTTGTTCTTGCAGGGGTAATAAATGCTCTAAAAGTAGTTAATAAGAAAATAGAAGACGTAAAGGT
TGTTGTAAATGGTGCAGGAGCTGCAGGAACTGCCATAACTAAACTATTACTTTCTTTAGGAGTAAAAAATCTAATTGTAT
GTGATAAGATTGGTATTCTTTATGAAGGAATAGAAAATGTAGATGATGCAAAGAAGGAACTTTCAAAGGTAACAAATCCA
GAAAATATTAAAGGAACTTTAGCTGATGCATTAATAGGTGCAGATGTATTTATAGGGGTTTCAGCTCCAGGAATAGTAAG
TAAAGATATGGTAAAAACAATGAATAAGGATTCTATAATATTTGCTATGGCAAACCCAACTCCAGAAATAATGCCAGATT
TGGCAAAAGAAGCAGGGGCTAAGGTAATTGGAACAGGAAGATCAGACTTCCCTAACCAAGTTAACAATGTGCTTGCATTC
CCAGGAATATTTAAGGGTGCATTGGATGTTAGAGCAAAAGAAATTAATGAAGAAATGAAAATAGCAGCAGCATATGCTAT
AGCTTCTATGATAAAAGATGAGGACTTAAATGAGGACAATGTAATTCATTATGCATTAGATAGATCTGTTGCAGACAATG
TAGCAAAAGCAATAAAAGAAGCAGCGAGAAAAAGCGGAGTAGCTAGAGTATAA

Upstream 100 bases:

>100_bases
TTTAAAGTTTAAAATATTTTAATAATTTAAACTTGATGTTATGCTTTATTCAAAGCAAACGATTACAGAAATCATATAAA
AGTGAAAGGATGTTATAAGA

Downstream 100 bases:

>100_bases
ACAGAATTCTTAGGAGTTTTACTCCTTAGAAGTCGTTATCCTTTAGGAGAAATCGTTATCCAGGGACGTAACCGCTCTTT
ACTCCCACTTTGAAGAAAAG

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MNYFEESLKLHEDNRGKIEIKSKMEVKTRDDLSLAYTPGVAEPCRKIHENEENVYKYTSKGNMVAVVTDGTAVLGLGDIG
PKAGLPVMEGKALLFKEFANVDAFPICLDTKDVDEIVKTVKLIAPGFGGINLEDIGAPRCFEIEEKLKKELDIPVFHDDQ
HGTAIVVLAGVINALKVVNKKIEDVKVVVNGAGAAGTAITKLLLSLGVKNLIVCDKIGILYEGIENVDDAKKELSKVTNP
ENIKGTLADALIGADVFIGVSAPGIVSKDMVKTMNKDSIIFAMANPTPEIMPDLAKEAGAKVIGTGRSDFPNQVNNVLAF
PGIFKGALDVRAKEINEEMKIAAAYAIASMIKDEDLNEDNVIHYALDRSVADNVAKAIKEAARKSGVARV

Sequences:

>Translated_390_residues
MNYFEESLKLHEDNRGKIEIKSKMEVKTRDDLSLAYTPGVAEPCRKIHENEENVYKYTSKGNMVAVVTDGTAVLGLGDIG
PKAGLPVMEGKALLFKEFANVDAFPICLDTKDVDEIVKTVKLIAPGFGGINLEDIGAPRCFEIEEKLKKELDIPVFHDDQ
HGTAIVVLAGVINALKVVNKKIEDVKVVVNGAGAAGTAITKLLLSLGVKNLIVCDKIGILYEGIENVDDAKKELSKVTNP
ENIKGTLADALIGADVFIGVSAPGIVSKDMVKTMNKDSIIFAMANPTPEIMPDLAKEAGAKVIGTGRSDFPNQVNNVLAF
PGIFKGALDVRAKEINEEMKIAAAYAIASMIKDEDLNEDNVIHYALDRSVADNVAKAIKEAARKSGVARV
>Mature_390_residues
MNYFEESLKLHEDNRGKIEIKSKMEVKTRDDLSLAYTPGVAEPCRKIHENEENVYKYTSKGNMVAVVTDGTAVLGLGDIG
PKAGLPVMEGKALLFKEFANVDAFPICLDTKDVDEIVKTVKLIAPGFGGINLEDIGAPRCFEIEEKLKKELDIPVFHDDQ
HGTAIVVLAGVINALKVVNKKIEDVKVVVNGAGAAGTAITKLLLSLGVKNLIVCDKIGILYEGIENVDDAKKELSKVTNP
ENIKGTLADALIGADVFIGVSAPGIVSKDMVKTMNKDSIIFAMANPTPEIMPDLAKEAGAKVIGTGRSDFPNQVNNVLAF
PGIFKGALDVRAKEINEEMKIAAAYAIASMIKDEDLNEDNVIHYALDRSVADNVAKAIKEAARKSGVARV

Specific function: In addition to the NAD-dependent oxidative decarboxylation of L-malate, the enzyme catalyzes the decarboxylation of oxaloacetate [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505145, Length=356, Percent_Identity=29.2134831460674, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI270265879, Length=320, Percent_Identity=30, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI4505143, Length=390, Percent_Identity=24.6153846153846, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI62420882, Length=384, Percent_Identity=24.7395833333333, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI62420880, Length=384, Percent_Identity=24.7395833333333, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI239049447, Length=384, Percent_Identity=24.7395833333333, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1788806, Length=403, Percent_Identity=49.1315136476427, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI87081919, Length=446, Percent_Identity=24.6636771300448, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17537199, Length=440, Percent_Identity=25, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6322823, Length=345, Percent_Identity=27.536231884058, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI281362672, Length=438, Percent_Identity=25.3424657534247, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356279, Length=438, Percent_Identity=25.3424657534247, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281362674, Length=438, Percent_Identity=25.3424657534247, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI78707242, Length=356, Percent_Identity=26.685393258427, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI78707238, Length=356, Percent_Identity=26.685393258427, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI78707240, Length=356, Percent_Identity=26.685393258427, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281363503, Length=391, Percent_Identity=27.1099744245524, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281363505, Length=391, Percent_Identity=27.1099744245524, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI78707236, Length=391, Percent_Identity=27.1099744245524, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI78707232, Length=391, Percent_Identity=27.1099744245524, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 41929; Mature: 41929

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYFEESLKLHEDNRGKIEIKSKMEVKTRDDLSLAYTPGVAEPCRKIHENEENVYKYTSK
CCHHHHHHHHCCCCCCEEEEECCEEEECCCCCEEEECCCHHHHHHHHHCCCCCEEEEECC
GNMVAVVTDGTAVLGLGDIGPKAGLPVMEGKALLFKEFANVDAFPICLDTKDVDEIVKTV
CCEEEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHH
KLIAPGFGGINLEDIGAPRCFEIEEKLKKELDIPVFHDDQHGTAIVVLAGVINALKVVNK
HHHCCCCCCCCHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHH
KIEDVKVVVNGAGAAGTAITKLLLSLGVKNLIVCDKIGILYEGIENVDDAKKELSKVTNP
HHCEEEEEEECCCCHHHHHHHHHHHHCHHHEEEHHHHHHHHHHHCCHHHHHHHHHHCCCC
ENIKGTLADALIGADVFIGVSAPGIVSKDMVKTMNKDSIIFAMANPTPEIMPDLAKEAGA
CCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCC
KVIGTGRSDFPNQVNNVLAFPGIFKGALDVRAKEINEEMKIAAAYAIASMIKDEDLNEDN
EEEECCCCCCCHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VIHYALDRSVADNVAKAIKEAARKSGVARV
EEEEEHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNYFEESLKLHEDNRGKIEIKSKMEVKTRDDLSLAYTPGVAEPCRKIHENEENVYKYTSK
CCHHHHHHHHCCCCCCEEEEECCEEEECCCCCEEEECCCHHHHHHHHHCCCCCEEEEECC
GNMVAVVTDGTAVLGLGDIGPKAGLPVMEGKALLFKEFANVDAFPICLDTKDVDEIVKTV
CCEEEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHH
KLIAPGFGGINLEDIGAPRCFEIEEKLKKELDIPVFHDDQHGTAIVVLAGVINALKVVNK
HHHCCCCCCCCHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHH
KIEDVKVVVNGAGAAGTAITKLLLSLGVKNLIVCDKIGILYEGIENVDDAKKELSKVTNP
HHCEEEEEEECCCCHHHHHHHHHHHHCHHHEEEHHHHHHHHHHHCCHHHHHHHHHHCCCC
ENIKGTLADALIGADVFIGVSAPGIVSKDMVKTMNKDSIIFAMANPTPEIMPDLAKEAGA
CCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCC
KVIGTGRSDFPNQVNNVLAFPGIFKGALDVRAKEINEEMKIAAAYAIASMIKDEDLNEDN
EEEECCCCCCCHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VIHYALDRSVADNVAKAIKEAARKSGVARV
EEEEEHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644282 [H]