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The map label for this gene is enr

Identifier: 170754444

GI number: 170754444

Start: 2350254

End: 2352251

Strand: Reverse

Name: enr

Synonym: CLD_2412

Alternate gene names: 170754444

Gene position: 2352251-2350254 (Counterclockwise)

Preceding gene: 170757681

Following gene: 170755957

Centisome position: 59.43

GC content: 34.88

Gene sequence:

>1998_bases
ATGAAGGATAAGTATAAGGTACTTTATGACCCAATTAAAATTGGAAAATTGGAGATTAAAAATAGATATGTTCTTGCTCC
AATGGGACCAGGAGGAATGTGTAACGCCGATGGTAGTTTTAATAAAAGAGGAATTGAGTTTTATGTAGAACGTGCAAAAG
GCGGAACTGGATTAATTATGACAGGTGTAACAATGGTGGAAAACAATATTGAAAAATGTGCATTACCATCCATGCCATGT
CCAACAATTAACCCTTTAAGCTTTATTACAACAGGTAATGAAATGACAGAAAGAGTCCATGCATATGGAGCAAAAATATT
TTTACAATTATCAGCAGGCTTTGGCCGAGTAAGTATACCATCTATTGTAGGAAAAGTGGCAGTAGCACCTTCTAAAATTC
CACATAGATTTTTACCAGGAGTAACCTGTCGTGAATTAACTACTGAAGAAGTAAAAGAATATGTAAAAGCATTTGGTGAG
TCAGCGGAAATCGCAAAAAAAGCCGGATTCGATGGTGTTGAAATTCATGCAGTACATGAAGGATATTTATTAGATCAATT
TGCTATTTCTTTCTTTAATCATCGTACTGATGAATATGGTGGATCATTAGAAAATCGCTTAAGATTTGCCTGTGAAGTTG
TACAAGAGATTAAGAAACGCTGTGGACAAGACTTCCCAGTTTCACTTAGATACAGTATTAAAAGCTTCATTAAGGACTGG
TGTAAAGGCGGTTTACCGGATGAAGAATTTGAAGAAAAGGGAAGAGATATTCCAGAAGGAATTGAAGCTGCAAAAATGCT
TGTTGCAGCAGGATATGATGCGCTAAACGGAGATGTCGGGTCCTATGATTCATGGTATTGGAGTCATCCACCAATGTATC
AAAAGAAGGGTTTATATCTTCCATATAATGAAATCCTTAAAAATGCAGTAGACGTACCTATTATTACCGCAGGAAGAATG
GAAGATCCTGAACTATCAAGTGATGCAATTTTGTCAGGAAAAACAGATATGATTGCTTTAGGAAGACCACTTCTTGCAGA
TGCAGAAATTCCAAATAAAATTTTTGAAGATAAATATGATAAAGTTAGACCTTGTTTATCTTGTCAGGAAGGATGTATGG
GAAGATTACAGAATTTTGCAACAGTATCCTGTGCAGTAAATCCTGCCTGTGGACGTGAAAAGGAGTATGGGCTAAAAAAA
GCAGAACAGATTAAAAAAGTTCTTGTTGTAGGTGGTGGTGTTGCAGGAATGGAAGCCGCAAGAGTTGCAGCTGTTCGTGG
ACACAAAGTAACATTGATTGAAAAGAATGGTTATCTTGGCGGAAATATTGTACCAGGAGGAGTTCCAGATTTCAAAGATG
ATGATCGTGCACTTGTTAAATGGTATGAGGGCATATTGAAAGATTTAGGTGTTGAAATAAAATTAAATGTGGATGCATCA
AAGGAAAATATCAAAGAATTTGGAGCTGATGAAGTACTTTTAGCAACAGGTTCTAGTCCAAGAACATTGACTATTGAAGG
ATCTGATAACGTTTATTCAGCAGAAGATGTGCTAATGGAAAGAAAAAATGTTGGTGAAAAGGTTATTGTGATTGGTGGAG
GACTTGTTGGATGTGAAACAGCTCTTTGGTTAAAACAACAAGGTAAAGAGGTTACAATTGTAGAGATGCAAAATGATATT
TTACAAGTAGGAGGACCTTTATGTCATGCAAACCATGATATGCTTAATGATTTAATTAAATTTAATAAGATTAATGTTAA
GACAGGCTCTTATATCAGCAAGAAAACAGATAAAGGTTTTGTTTTAAATACAGATGGACAAGAATCAATCATTAATGCTG
ATAGTGCTGTTGTAGCTATTGGATATTTATCTGAAAAAGACTTATATAATGAAGTTAGATTTGATATTCCAAATGCAAGA
CTAATTGGAGATGCTAATAAAGTTCAAAATATTATGTATGCTATTTGGAGTGCATATGAAGTAGCTAAAAATATTTAA

Upstream 100 bases:

>100_bases
ATAACAATATCCTCTTGAAAGTAAAGCTACTTTATGCTCTATACTGAAGGTAACAAGAAAATAAAAAAATACAAATGTGA
ATTCAGAAAGGAGAAAGAAA

Downstream 100 bases:

>100_bases
ATACACTTTTGCGAAAAACTAGAATGGATTTATATCCATATCTAGTTTTTGCTCTTTACTAAAATTTTATAAGTATAGTG
ATGTTTGTTATGTACTACTT

Product: 2-enoate reductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MKDKYKVLYDPIKIGKLEIKNRYVLAPMGPGGMCNADGSFNKRGIEFYVERAKGGTGLIMTGVTMVENNIEKCALPSMPC
PTINPLSFITTGNEMTERVHAYGAKIFLQLSAGFGRVSIPSIVGKVAVAPSKIPHRFLPGVTCRELTTEEVKEYVKAFGE
SAEIAKKAGFDGVEIHAVHEGYLLDQFAISFFNHRTDEYGGSLENRLRFACEVVQEIKKRCGQDFPVSLRYSIKSFIKDW
CKGGLPDEEFEEKGRDIPEGIEAAKMLVAAGYDALNGDVGSYDSWYWSHPPMYQKKGLYLPYNEILKNAVDVPIITAGRM
EDPELSSDAILSGKTDMIALGRPLLADAEIPNKIFEDKYDKVRPCLSCQEGCMGRLQNFATVSCAVNPACGREKEYGLKK
AEQIKKVLVVGGGVAGMEAARVAAVRGHKVTLIEKNGYLGGNIVPGGVPDFKDDDRALVKWYEGILKDLGVEIKLNVDAS
KENIKEFGADEVLLATGSSPRTLTIEGSDNVYSAEDVLMERKNVGEKVIVIGGGLVGCETALWLKQQGKEVTIVEMQNDI
LQVGGPLCHANHDMLNDLIKFNKINVKTGSYISKKTDKGFVLNTDGQESIINADSAVVAIGYLSEKDLYNEVRFDIPNAR
LIGDANKVQNIMYAIWSAYEVAKNI

Sequences:

>Translated_665_residues
MKDKYKVLYDPIKIGKLEIKNRYVLAPMGPGGMCNADGSFNKRGIEFYVERAKGGTGLIMTGVTMVENNIEKCALPSMPC
PTINPLSFITTGNEMTERVHAYGAKIFLQLSAGFGRVSIPSIVGKVAVAPSKIPHRFLPGVTCRELTTEEVKEYVKAFGE
SAEIAKKAGFDGVEIHAVHEGYLLDQFAISFFNHRTDEYGGSLENRLRFACEVVQEIKKRCGQDFPVSLRYSIKSFIKDW
CKGGLPDEEFEEKGRDIPEGIEAAKMLVAAGYDALNGDVGSYDSWYWSHPPMYQKKGLYLPYNEILKNAVDVPIITAGRM
EDPELSSDAILSGKTDMIALGRPLLADAEIPNKIFEDKYDKVRPCLSCQEGCMGRLQNFATVSCAVNPACGREKEYGLKK
AEQIKKVLVVGGGVAGMEAARVAAVRGHKVTLIEKNGYLGGNIVPGGVPDFKDDDRALVKWYEGILKDLGVEIKLNVDAS
KENIKEFGADEVLLATGSSPRTLTIEGSDNVYSAEDVLMERKNVGEKVIVIGGGLVGCETALWLKQQGKEVTIVEMQNDI
LQVGGPLCHANHDMLNDLIKFNKINVKTGSYISKKTDKGFVLNTDGQESIINADSAVVAIGYLSEKDLYNEVRFDIPNAR
LIGDANKVQNIMYAIWSAYEVAKNI
>Mature_665_residues
MKDKYKVLYDPIKIGKLEIKNRYVLAPMGPGGMCNADGSFNKRGIEFYVERAKGGTGLIMTGVTMVENNIEKCALPSMPC
PTINPLSFITTGNEMTERVHAYGAKIFLQLSAGFGRVSIPSIVGKVAVAPSKIPHRFLPGVTCRELTTEEVKEYVKAFGE
SAEIAKKAGFDGVEIHAVHEGYLLDQFAISFFNHRTDEYGGSLENRLRFACEVVQEIKKRCGQDFPVSLRYSIKSFIKDW
CKGGLPDEEFEEKGRDIPEGIEAAKMLVAAGYDALNGDVGSYDSWYWSHPPMYQKKGLYLPYNEILKNAVDVPIITAGRM
EDPELSSDAILSGKTDMIALGRPLLADAEIPNKIFEDKYDKVRPCLSCQEGCMGRLQNFATVSCAVNPACGREKEYGLKK
AEQIKKVLVVGGGVAGMEAARVAAVRGHKVTLIEKNGYLGGNIVPGGVPDFKDDDRALVKWYEGILKDLGVEIKLNVDAS
KENIKEFGADEVLLATGSSPRTLTIEGSDNVYSAEDVLMERKNVGEKVIVIGGGLVGCETALWLKQQGKEVTIVEMQNDI
LQVGGPLCHANHDMLNDLIKFNKINVKTGSYISKKTDKGFVLNTDGQESIINADSAVVAIGYLSEKDLYNEVRFDIPNAR
LIGDANKVQNIMYAIWSAYEVAKNI

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=564, Percent_Identity=32.8014184397163, Blast_Score=276, Evalue=4e-75,
Organism=Escherichia coli, GI1787939, Length=257, Percent_Identity=31.5175097276265, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17565138, Length=363, Percent_Identity=28.6501377410468, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17564188, Length=227, Percent_Identity=28.6343612334802, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=217, Percent_Identity=29.0322580645161, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=226, Percent_Identity=30.9734513274336, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=217, Percent_Identity=30.8755760368664, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=363, Percent_Identity=26.7217630853994, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17566914, Length=219, Percent_Identity=24.6575342465753, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6321973, Length=248, Percent_Identity=30.241935483871, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6325086, Length=242, Percent_Identity=29.7520661157025, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28574881, Length=173, Percent_Identity=32.9479768786127, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24665539, Length=173, Percent_Identity=32.9479768786127, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24665547, Length=173, Percent_Identity=32.9479768786127, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24665543, Length=173, Percent_Identity=32.9479768786127, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 72982; Mature: 72982

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDKYKVLYDPIKIGKLEIKNRYVLAPMGPGGMCNADGSFNKRGIEFYVERAKGGTGLIM
CCCCCEEEECCEEECEEEECCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEE
TGVTMVENNIEKCALPSMPCPTINPLSFITTGNEMTERVHAYGAKIFLQLSAGFGRVSIP
ECHHHHHCCHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHCEEEEEEEECCCCCEEHH
SIVGKVAVAPSKIPHRFLPGVTCRELTTEEVKEYVKAFGESAEIAKKAGFDGVEIHAVHE
HHHHHHHCCCCCCCHHHCCCCCHHHCCHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEEC
GYLLDQFAISFFNHRTDEYGGSLENRLRFACEVVQEIKKRCGQDFPVSLRYSIKSFIKDW
CHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHH
CKGGLPDEEFEEKGRDIPEGIEAAKMLVAAGYDALNGDVGSYDSWYWSHPPMYQKKGLYL
HCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCHHCCCCCC
PYNEILKNAVDVPIITAGRMEDPELSSDAILSGKTDMIALGRPLLADAEIPNKIFEDKYD
CHHHHHHHCCCCCEEECCCCCCCCCCCCCEECCCCCEEECCCCCCCCCCCCHHHHHHHHH
KVRPCLSCQEGCMGRLQNFATVSCAVNPACGREKEYGLKKAEQIKKVLVVGGGVAGMEAA
HHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHCCHHHHHHHEEEEECCCHHHHHHH
RVAAVRGHKVTLIEKNGYLGGNIVPGGVPDFKDDDRALVKWYEGILKDLGVEIKLNVDAS
HHHEECCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCC
KENIKEFGADEVLLATGSSPRTLTIEGSDNVYSAEDVLMERKNVGEKVIVIGGGLVGCET
HHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHH
ALWLKQQGKEVTIVEMQNDILQVGGPLCHANHDMLNDLIKFNKINVKTGSYISKKTDKGF
HHHHHHCCCEEEEEEECHHHHHHCCCEECCCHHHHHHHHHHCEEEECCCCCCCCCCCCCE
VLNTDGQESIINADSAVVAIGYLSEKDLYNEVRFDIPNARLIGDANKVQNIMYAIWSAYE
EEECCCCHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHH
VAKNI
HHHCC
>Mature Secondary Structure
MKDKYKVLYDPIKIGKLEIKNRYVLAPMGPGGMCNADGSFNKRGIEFYVERAKGGTGLIM
CCCCCEEEECCEEECEEEECCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEE
TGVTMVENNIEKCALPSMPCPTINPLSFITTGNEMTERVHAYGAKIFLQLSAGFGRVSIP
ECHHHHHCCHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHCEEEEEEEECCCCCEEHH
SIVGKVAVAPSKIPHRFLPGVTCRELTTEEVKEYVKAFGESAEIAKKAGFDGVEIHAVHE
HHHHHHHCCCCCCCHHHCCCCCHHHCCHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEEC
GYLLDQFAISFFNHRTDEYGGSLENRLRFACEVVQEIKKRCGQDFPVSLRYSIKSFIKDW
CHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHH
CKGGLPDEEFEEKGRDIPEGIEAAKMLVAAGYDALNGDVGSYDSWYWSHPPMYQKKGLYL
HCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCHHCCCCCC
PYNEILKNAVDVPIITAGRMEDPELSSDAILSGKTDMIALGRPLLADAEIPNKIFEDKYD
CHHHHHHHCCCCCEEECCCCCCCCCCCCCEECCCCCEEECCCCCCCCCCCCHHHHHHHHH
KVRPCLSCQEGCMGRLQNFATVSCAVNPACGREKEYGLKKAEQIKKVLVVGGGVAGMEAA
HHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHCCHHHHHHHEEEEECCCHHHHHHH
RVAAVRGHKVTLIEKNGYLGGNIVPGGVPDFKDDDRALVKWYEGILKDLGVEIKLNVDAS
HHHEECCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCC
KENIKEFGADEVLLATGSSPRTLTIEGSDNVYSAEDVLMERKNVGEKVIVIGGGLVGCET
HHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHH
ALWLKQQGKEVTIVEMQNDILQVGGPLCHANHDMLNDLIKFNKINVKTGSYISKKTDKGF
HHHHHHCCCEEEEEEECHHHHHHCCCEECCCHHHHHHHHHHCEEEECCCCCCCCCCCCCE
VLNTDGQESIINADSAVVAIGYLSEKDLYNEVRFDIPNARLIGDANKVQNIMYAIWSAYE
EEECCCCHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHH
VAKNI
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]