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The map label for this gene is hybA [H]

Identifier: 170754395

GI number: 170754395

Start: 1477152

End: 1477595

Strand: Direct

Name: hybA [H]

Synonym: CLD_3225

Alternate gene names: 170754395

Gene position: 1477152-1477595 (Clockwise)

Preceding gene: 170755337

Following gene: 170755183

Centisome position: 37.32

GC content: 31.76

Gene sequence:

>444_bases
ATGAGAAGAATTATGATAAAAAAGGATTTATGTGAAGGATGCCTAAATTGTGTTCTTGCCTGTATGTCAGAGCATAATAA
AATGGGGAAATCCATATATGATTTAGATTTAGAAGATAAGGTTAATGAAAGCAGAAATCATATAGAGTTAGATATGCTAG
GAAACAAAACCCCTATATTTTGTAGACATTGTGATAATCCAGAGTGTGTAAATACTTGTACAACTGGAGCTATGAGTAAA
GATGAAAAAACCGGTGTAGTATCTTATAATGAAGATAAATGTGCATCCTGTTTTATGTGTGTAATGAACTGCCCTTATGG
AGTACTAAAGGCAGATGAAGAAAACAGTAAAGTTATAATAAAATGTGATTTATGTAATGAAAGAGAAATACCTAGATGTG
TAGAAAACTGCCCTACAGGAGCTATTTATGTAGAGGAAATTTGA

Upstream 100 bases:

>100_bases
ATATTGTTTCGGATCAATGTAAAAATACTAACTATGGAATTAAACTAAAATTAGTTTTTGAAAGAATGTGGTTTTGAAAT
AAGTTAAGGGGGAATTCTTT

Downstream 100 bases:

>100_bases
ATATATACTAAAAAATTAAGTAAGGTATAATGTATTAGTTAAGGTTTTTATTAAAAATAATTAACATTTTATAATTTAAG
AGTATAGATTGTAAAGTAGG

Product: nitrate reductase, iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MRRIMIKKDLCEGCLNCVLACMSEHNKMGKSIYDLDLEDKVNESRNHIELDMLGNKTPIFCRHCDNPECVNTCTTGAMSK
DEKTGVVSYNEDKCASCFMCVMNCPYGVLKADEENSKVIIKCDLCNEREIPRCVENCPTGAIYVEEI

Sequences:

>Translated_147_residues
MRRIMIKKDLCEGCLNCVLACMSEHNKMGKSIYDLDLEDKVNESRNHIELDMLGNKTPIFCRHCDNPECVNTCTTGAMSK
DEKTGVVSYNEDKCASCFMCVMNCPYGVLKADEENSKVIIKCDLCNEREIPRCVENCPTGAIYVEEI
>Mature_147_residues
MRRIMIKKDLCEGCLNCVLACMSEHNKMGKSIYDLDLEDKVNESRNHIELDMLGNKTPIFCRHCDNPECVNTCTTGAMSK
DEKTGVVSYNEDKCASCFMCVMNCPYGVLKADEENSKVIIKCDLCNEREIPRCVENCPTGAIYVEEI

Specific function: Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=166, Percent_Identity=32.5301204819277, Blast_Score=81, Evalue=2e-17,
Organism=Escherichia coli, GI1788811, Length=151, Percent_Identity=30.4635761589404, Blast_Score=78, Evalue=2e-16,
Organism=Escherichia coli, GI87082179, Length=146, Percent_Identity=33.5616438356164, Blast_Score=77, Evalue=4e-16,
Organism=Escherichia coli, GI87082180, Length=137, Percent_Identity=31.3868613138686, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI2367345, Length=143, Percent_Identity=34.2657342657343, Blast_Score=73, Evalue=7e-15,
Organism=Escherichia coli, GI1787872, Length=148, Percent_Identity=34.4594594594595, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI1787122, Length=147, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=6e-14,
Organism=Escherichia coli, GI1789067, Length=159, Percent_Identity=31.4465408805031, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI1787749, Length=158, Percent_Identity=30.379746835443, Blast_Score=67, Evalue=4e-13,
Organism=Escherichia coli, GI2367245, Length=145, Percent_Identity=29.6551724137931, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI226510944, Length=152, Percent_Identity=30.9210526315789, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790326, Length=158, Percent_Identity=30.379746835443, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1787963, Length=153, Percent_Identity=26.797385620915, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 16606; Mature: 16606

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.9 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
16.3 %Cys+Met (Translated Protein)
10.9 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
16.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIMIKKDLCEGCLNCVLACMSEHNKMGKSIYDLDLEDKVNESRNHIELDMLGNKTPIF
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHCCCCCCEEEEEECCCCCCEE
CRHCDNPECVNTCTTGAMSKDEKTGVVSYNEDKCASCFMCVMNCPYGVLKADEENSKVII
EEECCCCHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEECCCCCCEEEE
KCDLCNEREIPRCVENCPTGAIYVEEI
EEECCCCCCCCHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MRRIMIKKDLCEGCLNCVLACMSEHNKMGKSIYDLDLEDKVNESRNHIELDMLGNKTPIF
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHCCCCCCEEEEEECCCCCCEE
CRHCDNPECVNTCTTGAMSKDEKTGVVSYNEDKCASCFMCVMNCPYGVLKADEENSKVII
EEECCCCHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEECCCCCCEEEE
KCDLCNEREIPRCVENCPTGAIYVEEI
EEECCCCCCCCHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]