Click here to switch to the map view.

The map label for this gene is supH [H]

Identifier: 170754328

GI number: 170754328

Start: 1299702

End: 1300496

Strand: Direct

Name: supH [H]

Synonym: CLD_3396

Alternate gene names: 170754328

Gene position: 1299702-1300496 (Clockwise)

Preceding gene: 170754644

Following gene: 170756980

Centisome position: 32.84

GC content: 25.66

Gene sequence:

>795_bases
ATGATAAAATTTATAGCTACAGATTTAGATGGAACACTAGTAAATAGTGAAGGTAAAATATATAATAAGGTGTTTAATTT
AATAAACGATTTACATAAAAATGGAGTAAAGTTTGCAGCAGCTAGTGGAAGATTTTATTCTCAATTAAATGAAAATTTCA
ATAGTGTAAAGGAAGATATGATACTTATAGCTCATAATGGAGCCTTAATAAAATATAGTAAAAATGGACAAACTCTTTAT
GCTAATTATATAGACAAAGAGTATATAAAATCAGTACAAAAATTAAAAAGAAATTTTGGAGAAGAATTGATTTTAGCAGG
AGAAAATGAAGCTTTCGTTGTAAATCCTTCTGAAAGTATTAAAGAAGAGTTTAGGTTTTATAATGTACCTTATATAGAAT
ACAAATCCTTTGATGAAGTGGATAAACCTGTTCAAAAGATAAGTTATTATGTTAAAGATGGTATTAAAGCACCTATGGTC
GATTATTTAAAAGAAAATTTAAATAAGAATCTTCAATTTGTTGCTTCAGGGGATAAATGGATAGATATGATGAATAAAGA
AGTAAGTAAAGGACATGCCATAAAAATACTTCAGAAAAAATTTAATATAGAAAAAGATAATACTATGGTTTTTGGAGATT
ATTATAATGATATAACCATGTTTAATCAAGCTTATTATAGTTATGCTATGGAAAATGCTCCAGAGGATGTAAAAGAAAAG
GCTAATTTTATAGCTGGTAACAATAACGAAAATGCAGTTTATAAAACCATAAGTAAACATATGGGTTTTATTTAA

Upstream 100 bases:

>100_bases
GTTGTGATATAATAGGAAAGTAGAAAAAACATATTTTTTAAATATAGAAATTATATGGGATTTTGAAATATAAAATTACC
CTAAAACATAGGAGGAAAAA

Downstream 100 bases:

>100_bases
TATAAAAAACTGTTTTAAAATATATTTTTGTCATAGGAGAATTTACTGATAAAATTTAATAACTATAAATAAACACTATC
AAAATCTATTAGAATTGATA

Product: HAD family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MIKFIATDLDGTLVNSEGKIYNKVFNLINDLHKNGVKFAAASGRFYSQLNENFNSVKEDMILIAHNGALIKYSKNGQTLY
ANYIDKEYIKSVQKLKRNFGEELILAGENEAFVVNPSESIKEEFRFYNVPYIEYKSFDEVDKPVQKISYYVKDGIKAPMV
DYLKENLNKNLQFVASGDKWIDMMNKEVSKGHAIKILQKKFNIEKDNTMVFGDYYNDITMFNQAYYSYAMENAPEDVKEK
ANFIAGNNNENAVYKTISKHMGFI

Sequences:

>Translated_264_residues
MIKFIATDLDGTLVNSEGKIYNKVFNLINDLHKNGVKFAAASGRFYSQLNENFNSVKEDMILIAHNGALIKYSKNGQTLY
ANYIDKEYIKSVQKLKRNFGEELILAGENEAFVVNPSESIKEEFRFYNVPYIEYKSFDEVDKPVQKISYYVKDGIKAPMV
DYLKENLNKNLQFVASGDKWIDMMNKEVSKGHAIKILQKKFNIEKDNTMVFGDYYNDITMFNQAYYSYAMENAPEDVKEK
ANFIAGNNNENAVYKTISKHMGFI
>Mature_264_residues
MIKFIATDLDGTLVNSEGKIYNKVFNLINDLHKNGVKFAAASGRFYSQLNENFNSVKEDMILIAHNGALIKYSKNGQTLY
ANYIDKEYIKSVQKLKRNFGEELILAGENEAFVVNPSESIKEEFRFYNVPYIEYKSFDEVDKPVQKISYYVKDGIKAPMV
DYLKENLNKNLQFVASGDKWIDMMNKEVSKGHAIKILQKKFNIEKDNTMVFGDYYNDITMFNQAYYSYAMENAPEDVKEK
ANFIAGNNNENAVYKTISKHMGFI

Specific function: Catalyzes the hydrolysis of sugar phosphate to sugar and inorganic phosphate. Has a wide substrate specificity catalyzing the hydrolysis of fructose-1-P most efficiently, but it remains uncertain if this is the real substrate in vivo [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family. SupH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787043, Length=264, Percent_Identity=29.1666666666667, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI87081790, Length=264, Percent_Identity=28.7878787878788, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI2367265, Length=268, Percent_Identity=25.7462686567164, Blast_Score=88, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF08282 Hydrolase_3 [H]

EC number: =3.1.3.23 [H]

Molecular weight: Translated: 30444; Mature: 30444

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKFIATDLDGTLVNSEGKIYNKVFNLINDLHKNGVKFAAASGRFYSQLNENFNSVKEDM
CCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCE
ILIAHNGALIKYSKNGQTLYANYIDKEYIKSVQKLKRNFGEELILAGENEAFVVNPSESI
EEEEECCEEEEECCCCCEEEHHHCCHHHHHHHHHHHHHCCCEEEEECCCCEEEECCHHHH
KEEFRFYNVPYIEYKSFDEVDKPVQKISYYVKDGIKAPMVDYLKENLNKNLQFVASGDKW
HHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCHHH
IDMMNKEVSKGHAIKILQKKFNIEKDNTMVFGDYYNDITMFNQAYYSYAMENAPEDVKEK
HHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHH
ANFIAGNNNENAVYKTISKHMGFI
HHEECCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKFIATDLDGTLVNSEGKIYNKVFNLINDLHKNGVKFAAASGRFYSQLNENFNSVKEDM
CCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCE
ILIAHNGALIKYSKNGQTLYANYIDKEYIKSVQKLKRNFGEELILAGENEAFVVNPSESI
EEEEECCEEEEECCCCCEEEHHHCCHHHHHHHHHHHHHCCCEEEEECCCCEEEECCHHHH
KEEFRFYNVPYIEYKSFDEVDKPVQKISYYVKDGIKAPMVDYLKENLNKNLQFVASGDKW
HHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCHHH
IDMMNKEVSKGHAIKILQKKFNIEKDNTMVFGDYYNDITMFNQAYYSYAMENAPEDVKEK
HHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHH
ANFIAGNNNENAVYKTISKHMGFI
HHEECCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]