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The map label for this gene is trmJ [H]

Identifier: 170752133

GI number: 170752133

Start: 6075134

End: 6075919

Strand: Direct

Name: trmJ [H]

Synonym: Mrad2831_5766

Alternate gene names: 170752133

Gene position: 6075134-6075919 (Clockwise)

Preceding gene: 170752131

Following gene: 170752135

Centisome position: 99.96

GC content: 71.63

Gene sequence:

>786_bases
ATGACGAGCCGAGACGCACAGGGCGACGCCCCCGAGGTTGCCCCCAGGAAGGTCACCGAACTGCCGCCCGGGATCGCCCC
CGCGGTGATCCTGGTGGAGCCGCAGCTTGCCGAGAATATCGGCATGACCGCCCGGGCCATGGCGAATTTCGGCCTGTCGG
AGCTGCGCCTCGTCAACCCGAAGAACGGCTGGCCCAAGAAGGGCGTCCGAGAGGCGGCCTCGGGCGCGACGCACGTCTTG
GACGCGGCCGCGATCTACGGCAGCGTGGCCGAGGCCATCGCTGACTGCCAGTACGTCCTGGCGACCACGGCGCGCGAGCG
CGGGCAGATGAAGCGGGTCTTCGCGCCCGAGGAGGCCATGGGCGAGCTCGTGGCCCGGGAGGGGCAGCGCACCGCGGTGA
TGTTCGGCCGCGAGCGGGTCGGGCTCACCAACGACGAGGTGTCGCTCGCCGACGCGATCGTCACCTTCCCGGTCTCCCCG
GACTTTCCCTCGCTCAACCTCGCGCAGGCGGTTCTGCTGGTGGGCTACGCGTGGCGGCAGGCGAGCGGCCGGGCGCGCCT
GCCCTTCACGGGCGAACTCCTGTCGCCGCCGGCGACCCGCGAGGCGCTGATCGCGCTGTTCGGAAGCCTTGAGGCGGCGC
TCGACGGGGCCGGCTTCTACCCGCCGGAGAAGAAGGAGATCATCGCCCGCAACATGCGCGACATGCTCCACCGCATGAGC
CTGACCGAGCAGGACGTGCGGACGTTCCGCGGGGCGCTGCGGGCCCTGACGCGGAAGGGCGGCTGA

Upstream 100 bases:

>100_bases
TAGCGCGGCCCTTCCGGCGACGGAAGGCGCGCGCTGTCATCCGGGCGGCATCGTCACTCGCCCCTCGACGGGCGCGGGCT
CGCCGTGCCAGACGGCCGCC

Downstream 100 bases:

>100_bases
TCAGCCGGGGGCCGGCTTCATCTCCGGCGGCTTGCCGCCGCCGAAGCAGCGGCCGACCGCCTCCCAGAACGCCGTCCGCT
CCTCGGGCGTGCACTGCGCC

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTSRDAQGDAPEVAPRKVTELPPGIAPAVILVEPQLAENIGMTARAMANFGLSELRLVNPKNGWPKKGVREAASGATHVL
DAAAIYGSVAEAIADCQYVLATTARERGQMKRVFAPEEAMGELVAREGQRTAVMFGRERVGLTNDEVSLADAIVTFPVSP
DFPSLNLAQAVLLVGYAWRQASGRARLPFTGELLSPPATREALIALFGSLEAALDGAGFYPPEKKEIIARNMRDMLHRMS
LTEQDVRTFRGALRALTRKGG

Sequences:

>Translated_261_residues
MTSRDAQGDAPEVAPRKVTELPPGIAPAVILVEPQLAENIGMTARAMANFGLSELRLVNPKNGWPKKGVREAASGATHVL
DAAAIYGSVAEAIADCQYVLATTARERGQMKRVFAPEEAMGELVAREGQRTAVMFGRERVGLTNDEVSLADAIVTFPVSP
DFPSLNLAQAVLLVGYAWRQASGRARLPFTGELLSPPATREALIALFGSLEAALDGAGFYPPEKKEIIARNMRDMLHRMS
LTEQDVRTFRGALRALTRKGG
>Mature_260_residues
TSRDAQGDAPEVAPRKVTELPPGIAPAVILVEPQLAENIGMTARAMANFGLSELRLVNPKNGWPKKGVREAASGATHVLD
AAAIYGSVAEAIADCQYVLATTARERGQMKRVFAPEEAMGELVAREGQRTAVMFGRERVGLTNDEVSLADAIVTFPVSPD
FPSLNLAQAVLLVGYAWRQASGRARLPFTGELLSPPATREALIALFGSLEAALDGAGFYPPEKKEIIARNMRDMLHRMSL
TEQDVRTFRGALRALTRKGG

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=216, Percent_Identity=33.7962962962963, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788881, Length=237, Percent_Identity=32.0675105485232, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28068; Mature: 27936

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRDAQGDAPEVAPRKVTELPPGIAPAVILVEPQLAENIGMTARAMANFGLSELRLVNP
CCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEECCHHHHHCCHHHHHHHHCCHHHEEEECC
KNGWPKKGVREAASGATHVLDAAAIYGSVAEAIADCQYVLATTARERGQMKRVFAPEEAM
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
GELVAREGQRTAVMFGRERVGLTNDEVSLADAIVTFPVSPDFPSLNLAQAVLLVGYAWRQ
HHHHHHCCCCEEEEECCHHCCCCCCCHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHH
ASGRARLPFTGELLSPPATREALIALFGSLEAALDGAGFYPPEKKEIIARNMRDMLHRMS
CCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
LTEQDVRTFRGALRALTRKGG
CCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSRDAQGDAPEVAPRKVTELPPGIAPAVILVEPQLAENIGMTARAMANFGLSELRLVNP
CCCCCCCCCCCCCCCHHHHCCCCCCCEEEEECCHHHHHCCHHHHHHHHCCHHHEEEECC
KNGWPKKGVREAASGATHVLDAAAIYGSVAEAIADCQYVLATTARERGQMKRVFAPEEAM
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
GELVAREGQRTAVMFGRERVGLTNDEVSLADAIVTFPVSPDFPSLNLAQAVLLVGYAWRQ
HHHHHHCCCCEEEEECCHHCCCCCCCHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHH
ASGRARLPFTGELLSPPATREALIALFGSLEAALDGAGFYPPEKKEIIARNMRDMLHRMS
CCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
LTEQDVRTFRGALRALTRKGG
CCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA