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The map label for this gene is aceF [H]

Identifier: 170751980

GI number: 170751980

Start: 5942503

End: 5943864

Strand: Reverse

Name: aceF [H]

Synonym: Mrad2831_5612

Alternate gene names: 170751980

Gene position: 5943864-5942503 (Counterclockwise)

Preceding gene: 170751981

Following gene: 170751979

Centisome position: 97.8

GC content: 72.1

Gene sequence:

>1362_bases
ATGAGCGCCGCGCTGCAGATCGCCCTTCCGGATATCGGCGACTACCGGGACGTGCCGGTCATCGAGCTTCTGGTGAAGCC
CGGCGACCGTCTCGCGGTGGACGACCTGATCCTGAGCATCGAGTCCGACAAGGCCACCATGGAGGTCCCCTCCCCGGTCG
CCGGCATCGTGCGCGAGTTGCTGGTCGCGGTGGGGTCGAAGGTGTCGGAGGGAACGCCGATCCTGACGGTGGAGCCCGCG
GAGGGCGGCGGGGCCGCACCGGTGCCCGACACATCGCCCGCTGTCGCGGCCTCGGCGCCCCCCGACCGGAGCGCGGCGCC
CCTCTCCCGTGCGGGAGAGGGACAGGGTGAGAGATCAGGGCATTCCGGAGACGGCGCCCCCCTCACCCCGCCCCTCTCCC
GGACGGCAGACGGAGTCGGTCGCGCCTCGTCGGAGCCGATTCAGGCGACCCGCGGCGACGTGCATGCCAGCCCGTCCGTC
CGCCAGCTCGCCCGCGAGCTCGGCGTGGGTCTCGACCGCGTCGCCGCCACCGGGCCGAAGGGGCGGATCCTGCGCGAGGA
CGTGCACGCCTTCGTCAAGGCGGCCCTCGGTGCGCCGGCCCAGGCGCCCGTCGCCGCGTCCGGGATCGGCCCCGGCCTAC
CGCCCTGGCCGGAGGTGGACTTCGCCAAGTACGGGCCGGTCCGGCGCGAACCGTTGTCGCGCATCCAGAGCCTCTCGGGG
GCGAATCTCAGCCGCAACTGGCTGACGATCCCGCACGTGACCAATTTCGACCGTGCCGACGTCACCGAGATCGAGTCGTT
CCGGCTCGGCCTGAACAAGGAGACGCGCACGCCGCCTGCCCGGGTCACCATGGTCGCCTTCCTGATCAAAGCGGCGGCCT
CGGCGCTGCGCGCCTACCCGCGATTCAACGCCTCGCTCGAGGGCGGCGACCTCGTCCTGAAGGACTACGTCCATGTCGGC
TTCGCGGTGGATACGCCGAAGGGCCTGATGGTGCCGGTGGTGCGCGACTGCGACCGCAAGGGCCTCATCGAGATCGCCAC
CGAGATGGCCGCGATGGCCGAGAAGGCCAGGTCCGGAACCCTTCCGGGCTCCGACATGCAGGGCGGCTGCTTCTCGGTCT
CGTCGCTCGGCGGCATCGGCGGCGACGGCTTCACCCCGATCATCAACGCCCCCGAGGTCGCCATCCTGGGCGCGGCGCGC
TCCCGCACCGAGGCGGTGTGGGACGGCAAAGCCTTCCAGCCGCGCCTGATCCTGCCGCTCAGCCTCTCTTGGGACCATCG
CGTCGTGGACGGCGTCGCCGCCGCCCGCTTCCTCGGCCACGTGGCGTCGGTCCTGTCGGACCTGCGCCGCGCCCTCCTGT
GA

Upstream 100 bases:

>100_bases
CGCGCCCGCGCCCTGGACGATCTGAGGCCGGGCGCCCCGCCGAGGGGCGCCGCCCGTCCCACCCCTTCTCCGAACGCCCG
ACCTACGGACATCCGACCGC

Downstream 100 bases:

>100_bases
CGGCCGCCGCCATGCCCGAGACCTTCGACATCCGCGTCCCCGATCTCGGGGACTACGCCGACGTGCCCGTCATCGAGATC
CCGGTGGCGCCCGGCCAGCC

Product: dehydrogenase catalytic domain-containing protein

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSAALQIALPDIGDYRDVPVIELLVKPGDRLAVDDLILSIESDKATMEVPSPVAGIVRELLVAVGSKVSEGTPILTVEPA
EGGGAAPVPDTSPAVAASAPPDRSAAPLSRAGEGQGERSGHSGDGAPLTPPLSRTADGVGRASSEPIQATRGDVHASPSV
RQLARELGVGLDRVAATGPKGRILREDVHAFVKAALGAPAQAPVAASGIGPGLPPWPEVDFAKYGPVRREPLSRIQSLSG
ANLSRNWLTIPHVTNFDRADVTEIESFRLGLNKETRTPPARVTMVAFLIKAAASALRAYPRFNASLEGGDLVLKDYVHVG
FAVDTPKGLMVPVVRDCDRKGLIEIATEMAAMAEKARSGTLPGSDMQGGCFSVSSLGGIGGDGFTPIINAPEVAILGAAR
SRTEAVWDGKAFQPRLILPLSLSWDHRVVDGVAAARFLGHVASVLSDLRRALL

Sequences:

>Translated_453_residues
MSAALQIALPDIGDYRDVPVIELLVKPGDRLAVDDLILSIESDKATMEVPSPVAGIVRELLVAVGSKVSEGTPILTVEPA
EGGGAAPVPDTSPAVAASAPPDRSAAPLSRAGEGQGERSGHSGDGAPLTPPLSRTADGVGRASSEPIQATRGDVHASPSV
RQLARELGVGLDRVAATGPKGRILREDVHAFVKAALGAPAQAPVAASGIGPGLPPWPEVDFAKYGPVRREPLSRIQSLSG
ANLSRNWLTIPHVTNFDRADVTEIESFRLGLNKETRTPPARVTMVAFLIKAAASALRAYPRFNASLEGGDLVLKDYVHVG
FAVDTPKGLMVPVVRDCDRKGLIEIATEMAAMAEKARSGTLPGSDMQGGCFSVSSLGGIGGDGFTPIINAPEVAILGAAR
SRTEAVWDGKAFQPRLILPLSLSWDHRVVDGVAAARFLGHVASVLSDLRRALL
>Mature_452_residues
SAALQIALPDIGDYRDVPVIELLVKPGDRLAVDDLILSIESDKATMEVPSPVAGIVRELLVAVGSKVSEGTPILTVEPAE
GGGAAPVPDTSPAVAASAPPDRSAAPLSRAGEGQGERSGHSGDGAPLTPPLSRTADGVGRASSEPIQATRGDVHASPSVR
QLARELGVGLDRVAATGPKGRILREDVHAFVKAALGAPAQAPVAASGIGPGLPPWPEVDFAKYGPVRREPLSRIQSLSGA
NLSRNWLTIPHVTNFDRADVTEIESFRLGLNKETRTPPARVTMVAFLIKAAASALRAYPRFNASLEGGDLVLKDYVHVGF
AVDTPKGLMVPVVRDCDRKGLIEIATEMAAMAEKARSGTLPGSDMQGGCFSVSSLGGIGGDGFTPIINAPEVAILGAARS
RTEAVWDGKAFQPRLILPLSLSWDHRVVDGVAAARFLGHVASVLSDLRRALL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=451, Percent_Identity=30.1552106430155, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI110671329, Length=468, Percent_Identity=26.4957264957265, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI203098816, Length=460, Percent_Identity=28.0434782608696, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI203098753, Length=460, Percent_Identity=28.0434782608696, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI19923748, Length=230, Percent_Identity=32.6086956521739, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI260898739, Length=153, Percent_Identity=37.2549019607843, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1786305, Length=452, Percent_Identity=46.0176991150442, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1786946, Length=456, Percent_Identity=28.9473684210526, Blast_Score=151, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17537937, Length=459, Percent_Identity=28.322440087146, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17560088, Length=458, Percent_Identity=29.6943231441048, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25146366, Length=203, Percent_Identity=34.9753694581281, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17538894, Length=301, Percent_Identity=31.2292358803987, Blast_Score=114, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=432, Percent_Identity=27.3148148148148, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6324258, Length=443, Percent_Identity=27.0880361173815, Blast_Score=133, Evalue=7e-32,
Organism=Drosophila melanogaster, GI18859875, Length=455, Percent_Identity=31.2087912087912, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI20129315, Length=222, Percent_Identity=36.036036036036, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24582497, Length=222, Percent_Identity=36.036036036036, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24645909, Length=221, Percent_Identity=31.6742081447964, Blast_Score=119, Evalue=5e-27,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 47136; Mature: 47005

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAALQIALPDIGDYRDVPVIELLVKPGDRLAVDDLILSIESDKATMEVPSPVAGIVREL
CCCEEEEECCCCCCCCCCCCEEEEECCCCCEEHHHEEEEECCCCCEEECCCHHHHHHHHH
LVAVGSKVSEGTPILTVEPAEGGGAAPVPDTSPAVAASAPPDRSAAPLSRAGEGQGERSG
HHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCC
HSGDGAPLTPPLSRTADGVGRASSEPIQATRGDVHASPSVRQLARELGVGLDRVAATGPK
CCCCCCCCCCCHHHHHHCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCC
GRILREDVHAFVKAALGAPAQAPVAASGIGPGLPPWPEVDFAKYGPVRREPLSRIQSLSG
CCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCC
ANLSRNWLTIPHVTNFDRADVTEIESFRLGLNKETRTPPARVTMVAFLIKAAASALRAYP
CCCCCCEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RFNASLEGGDLVLKDYVHVGFAVDTPKGLMVPVVRDCDRKGLIEIATEMAAMAEKARSGT
CCCCCCCCCCEEEEEHHEEEEEEECCCCCEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
LPGSDMQGGCFSVSSLGGIGGDGFTPIINAPEVAILGAARSRTEAVWDGKAFQPRLILPL
CCCCCCCCCEEEHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCCCCCEEEEEE
SLSWDHRVVDGVAAARFLGHVASVLSDLRRALL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SAALQIALPDIGDYRDVPVIELLVKPGDRLAVDDLILSIESDKATMEVPSPVAGIVREL
CCEEEEECCCCCCCCCCCCEEEEECCCCCEEHHHEEEEECCCCCEEECCCHHHHHHHHH
LVAVGSKVSEGTPILTVEPAEGGGAAPVPDTSPAVAASAPPDRSAAPLSRAGEGQGERSG
HHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCC
HSGDGAPLTPPLSRTADGVGRASSEPIQATRGDVHASPSVRQLARELGVGLDRVAATGPK
CCCCCCCCCCCHHHHHHCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCC
GRILREDVHAFVKAALGAPAQAPVAASGIGPGLPPWPEVDFAKYGPVRREPLSRIQSLSG
CCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCC
ANLSRNWLTIPHVTNFDRADVTEIESFRLGLNKETRTPPARVTMVAFLIKAAASALRAYP
CCCCCCEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RFNASLEGGDLVLKDYVHVGFAVDTPKGLMVPVVRDCDRKGLIEIATEMAAMAEKARSGT
CCCCCCCCCCEEEEEHHEEEEEEECCCCCEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
LPGSDMQGGCFSVSSLGGIGGDGFTPIINAPEVAILGAARSRTEAVWDGKAFQPRLILPL
CCCCCCCCCEEEHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCCCCCEEEEEE
SLSWDHRVVDGVAAARFLGHVASVLSDLRRALL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]