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The map label for this gene is lpdA [H]

Identifier: 170751979

GI number: 170751979

Start: 5940770

End: 5942491

Strand: Reverse

Name: lpdA [H]

Synonym: Mrad2831_5611

Alternate gene names: 170751979

Gene position: 5942491-5940770 (Counterclockwise)

Preceding gene: 170751980

Following gene: 170751978

Centisome position: 97.77

GC content: 74.33

Gene sequence:

>1722_bases
ATGCCCGAGACCTTCGACATCCGCGTCCCCGATCTCGGGGACTACGCCGACGTGCCCGTCATCGAGATCCCGGTGGCGCC
CGGCCAGCCGGTCGACAAGGACCAGACCCTACTGGTGGTCGAGTCCGACAAGGCGACGCTGGACATCCCTTCCCCAGCGG
CTGGCCGCCTCGTGGAGATGCTGGTCGGCCTCGGCGACACGGTCTCGGCCGGCACCCTCGTCGCCCGCCTCGCGGTCGAG
GCGCCCGGCGCGCGCGCCGAGCCGCTCACCGCTCCGGTCCCCGCGCCGCGCCCCGCCGCGCCGCCGCCGGAGCCCCGGGC
GGAGGCGGGCTGCGACCTCCTGGTGATCGGCGGCGGACCGGGCGGCTACTCGGCCGCCTTCCGGGCCGCCGATCTCGGGC
AGCGGGTGGTCCTGGTCGAGCGCCACGCGACCCTCGGCGGCGTCTGCCTCAACGTGGGCTGCATCCCGTCGAAAGCGCTG
CTGCACGTGGCCGCGGTCACCGAGGAGGCCGTCCGGCTGGCCGCGCACGGGATCAGCTTCGGCGCGCCGACGGTCGATCT
GGAGAAGCTGCGGACCTTCAAGGCCGGCACTGTCCGCCGCCTCACCGACGGGCTCGCCCAGATGGCGCGCCAGCGCAAGG
TCGAGGTGGTCCGCGGGACCGCCCGCTTCATCGGGCCCGACACGGTCTCGGTGACGCTGCACGACGGCGGCACGCGCGAC
ATCACCTTCACGGGCGCCGTCGTGGCGGCGGGCTCGGCGCCGGTGGCCCTGCCGATGCTCCCGGACGACCCGCGGATCGT
GAATTCCACCGGGGCGCTGGAACTGCCCGCCGTGCCGCGTCGGCTCCTCGTGATCGGCGGCGGCATCATCGGCCTGGAGA
TGGCGACCGTCTACAGCGCGCTCGGCGCGCGGGTCGACGTGGTCGAGCGCTTGCCCGGCCTTCTCGAGGGCGTCGACCGG
GATCTCGTCGGCGTCTGGACGAAGCGCAACGCCCACCGTTTCGACCGCGTCCTGACGGGGGTCGAGGTCGTCTCCGCCGA
GGCGAGACCGGACGGGATCGCCGTGACCTTCTCGGGTGAGGCCGAGCCCGGAACCTACGACCTCGTGCTCCAGGCCGCAG
GCCGCCGGCCCAACGGGGCGACGCTCGGTCTCGACGCGGCCGGCGTGGCGCTGGACGGCGGCTTCGTGCCGGTGGACGCC
CAGATGCGCACGAACGTGCCGCACATCTTCGCCATCGGCGATTGCGTCGGCCAGCCGATGCTCGCCCACAAGGCGGTGCA
TCAGGGCCACGTCGCCGCCGAGGCGGCCGCCGGCCACAAGGCCGGGTTCGACGCGGCCGTCATCCCCTCGGTCGCCTACA
CCGACCCGGAGATCGCCTGGGCCGGACTCACCGAGACGGCCGCGAAGGCGGCCGGGCGGGCCGTGGAGGTCGCGCGCTTC
CCCTGGGCCGCCTCGGGTCGGGCCATCGCCAACGGCGCCGATTACGGTCTGACGAAGCTGCTGTTCGACGCGCAGACCGG
GCGCGTCGTCGGCGGCGCCATCGTCGGTCCGAGCGCCGGCGACATGATCGGCGAGGTCGCGCTGGCGATCGAGATGGGCG
CCGACGCCACCGATATCGGCCGGACGATCCACCCCCACCCGACACTCGGCGAGACCATCGGACTCGCGGCGGAAGCCGGC
CTCGGCCTCTGCACCGACCTGCCCCCGTTGGCCCGTCGGTAG

Upstream 100 bases:

>100_bases
ACCATCGCGTCGTGGACGGCGTCGCCGCCGCCCGCTTCCTCGGCCACGTGGCGTCGGTCCTGTCGGACCTGCGCCGCGCC
CTCCTGTGACGGCCGCCGCC

Downstream 100 bases:

>100_bases
GCGCGTCGACGGGCCCAAACTCGGCCGTCCCGACGCGCGGCCGGCTCGACGGATCGCCGCGTGTTGTCTAGATCTCGCCC
TCCGTCGCCGGCTCGGCCGG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MPETFDIRVPDLGDYADVPVIEIPVAPGQPVDKDQTLLVVESDKATLDIPSPAAGRLVEMLVGLGDTVSAGTLVARLAVE
APGARAEPLTAPVPAPRPAAPPPEPRAEAGCDLLVIGGGPGGYSAAFRAADLGQRVVLVERHATLGGVCLNVGCIPSKAL
LHVAAVTEEAVRLAAHGISFGAPTVDLEKLRTFKAGTVRRLTDGLAQMARQRKVEVVRGTARFIGPDTVSVTLHDGGTRD
ITFTGAVVAAGSAPVALPMLPDDPRIVNSTGALELPAVPRRLLVIGGGIIGLEMATVYSALGARVDVVERLPGLLEGVDR
DLVGVWTKRNAHRFDRVLTGVEVVSAEARPDGIAVTFSGEAEPGTYDLVLQAAGRRPNGATLGLDAAGVALDGGFVPVDA
QMRTNVPHIFAIGDCVGQPMLAHKAVHQGHVAAEAAAGHKAGFDAAVIPSVAYTDPEIAWAGLTETAAKAAGRAVEVARF
PWAASGRAIANGADYGLTKLLFDAQTGRVVGGAIVGPSAGDMIGEVALAIEMGADATDIGRTIHPHPTLGETIGLAAEAG
LGLCTDLPPLARR

Sequences:

>Translated_573_residues
MPETFDIRVPDLGDYADVPVIEIPVAPGQPVDKDQTLLVVESDKATLDIPSPAAGRLVEMLVGLGDTVSAGTLVARLAVE
APGARAEPLTAPVPAPRPAAPPPEPRAEAGCDLLVIGGGPGGYSAAFRAADLGQRVVLVERHATLGGVCLNVGCIPSKAL
LHVAAVTEEAVRLAAHGISFGAPTVDLEKLRTFKAGTVRRLTDGLAQMARQRKVEVVRGTARFIGPDTVSVTLHDGGTRD
ITFTGAVVAAGSAPVALPMLPDDPRIVNSTGALELPAVPRRLLVIGGGIIGLEMATVYSALGARVDVVERLPGLLEGVDR
DLVGVWTKRNAHRFDRVLTGVEVVSAEARPDGIAVTFSGEAEPGTYDLVLQAAGRRPNGATLGLDAAGVALDGGFVPVDA
QMRTNVPHIFAIGDCVGQPMLAHKAVHQGHVAAEAAAGHKAGFDAAVIPSVAYTDPEIAWAGLTETAAKAAGRAVEVARF
PWAASGRAIANGADYGLTKLLFDAQTGRVVGGAIVGPSAGDMIGEVALAIEMGADATDIGRTIHPHPTLGETIGLAAEAG
LGLCTDLPPLARR
>Mature_572_residues
PETFDIRVPDLGDYADVPVIEIPVAPGQPVDKDQTLLVVESDKATLDIPSPAAGRLVEMLVGLGDTVSAGTLVARLAVEA
PGARAEPLTAPVPAPRPAAPPPEPRAEAGCDLLVIGGGPGGYSAAFRAADLGQRVVLVERHATLGGVCLNVGCIPSKALL
HVAAVTEEAVRLAAHGISFGAPTVDLEKLRTFKAGTVRRLTDGLAQMARQRKVEVVRGTARFIGPDTVSVTLHDGGTRDI
TFTGAVVAAGSAPVALPMLPDDPRIVNSTGALELPAVPRRLLVIGGGIIGLEMATVYSALGARVDVVERLPGLLEGVDRD
LVGVWTKRNAHRFDRVLTGVEVVSAEARPDGIAVTFSGEAEPGTYDLVLQAAGRRPNGATLGLDAAGVALDGGFVPVDAQ
MRTNVPHIFAIGDCVGQPMLAHKAVHQGHVAAEAAAGHKAGFDAAVIPSVAYTDPEIAWAGLTETAAKAAGRAVEVARFP
WAASGRAIANGADYGLTKLLFDAQTGRVVGGAIVGPSAGDMIGEVALAIEMGADATDIGRTIHPHPTLGETIGLAAEAGL
GLCTDLPPLARR

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=469, Percent_Identity=41.36460554371, Blast_Score=319, Evalue=4e-87,
Organism=Homo sapiens, GI50301238, Length=465, Percent_Identity=28.6021505376344, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI33519430, Length=452, Percent_Identity=28.0973451327434, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI33519428, Length=452, Percent_Identity=28.0973451327434, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI33519426, Length=452, Percent_Identity=28.0973451327434, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI148277071, Length=451, Percent_Identity=28.159645232816, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI148277065, Length=452, Percent_Identity=28.0973451327434, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI291045266, Length=474, Percent_Identity=27.8481012658228, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI22035672, Length=455, Percent_Identity=28.1318681318681, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI291045268, Length=466, Percent_Identity=27.2532188841202, Blast_Score=122, Evalue=1e-27,
Organism=Escherichia coli, GI1786307, Length=472, Percent_Identity=58.4745762711864, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI87082354, Length=478, Percent_Identity=29.7071129707113, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1789915, Length=447, Percent_Identity=29.9776286353468, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI87081717, Length=449, Percent_Identity=27.1714922048998, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1786305, Length=74, Percent_Identity=43.2432432432432, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=462, Percent_Identity=42.2077922077922, Blast_Score=327, Evalue=8e-90,
Organism=Caenorhabditis elegans, GI17557007, Length=473, Percent_Identity=29.8097251585624, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI71983419, Length=438, Percent_Identity=25.5707762557078, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71983429, Length=438, Percent_Identity=25.5707762557078, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71982272, Length=478, Percent_Identity=26.1506276150628, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=38.5964912280702, Blast_Score=270, Evalue=5e-73,
Organism=Saccharomyces cerevisiae, GI6325240, Length=478, Percent_Identity=28.0334728033473, Blast_Score=178, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6325166, Length=461, Percent_Identity=26.8980477223427, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21358499, Length=451, Percent_Identity=41.4634146341463, Blast_Score=328, Evalue=6e-90,
Organism=Drosophila melanogaster, GI24640549, Length=466, Percent_Identity=31.1158798283262, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24640553, Length=466, Percent_Identity=31.1158798283262, Blast_Score=131, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24640551, Length=466, Percent_Identity=30.68669527897, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17737741, Length=468, Percent_Identity=29.7008547008547, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 58665; Mature: 58534

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETFDIRVPDLGDYADVPVIEIPVAPGQPVDKDQTLLVVESDKATLDIPSPAAGRLVEM
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCEEECCCCCHHHHHHH
LVGLGDTVSAGTLVARLAVEAPGARAEPLTAPVPAPRPAAPPPEPRAEAGCDLLVIGGGP
HHCCCCCCCHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEECCCC
GGYSAAFRAADLGQRVVLVERHATLGGVCLNVGCIPSKALLHVAAVTEEAVRLAAHGISF
CCHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
GAPTVDLEKLRTFKAGTVRRLTDGLAQMARQRKVEVVRGTARFIGPDTVSVTLHDGGTRD
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECHHHCCCCCEEEEEEECCCCCE
ITFTGAVVAAGSAPVALPMLPDDPRIVNSTGALELPAVPRRLLVIGGGIIGLEMATVYSA
EEEECEEEECCCCCEEEECCCCCCCEECCCCCEECCCCCCEEEEEECCCHHHHHHHHHHH
LGARVDVVERLPGLLEGVDRDLVGVWTKRNAHRFDRVLTGVEVVSAEARPDGIAVTFSGE
HCCCHHHHHHCCHHHHHCCCCCEEEECCCCHHHHHHHHHHHHEEECCCCCCCEEEEECCC
AEPGTYDLVLQAAGRRPNGATLGLDAAGVALDGGFVPVDAQMRTNVPHIFAIGDCVGQPM
CCCCEEEEEEEECCCCCCCCEEECCCCCEEECCCEEEECCHHHCCCCEEEEEHHHHCCCH
LAHKAVHQGHVAAEAAAGHKAGFDAAVIPSVAYTDPEIAWAGLTETAAKAAGRAVEVARF
HHHHHHHCCCHHHHHCCCCCCCCCCCCCCCEECCCCCEEECCCHHHHHHHCCCEEEEEEC
PWAASGRAIANGADYGLTKLLFDAQTGRVVGGAIVGPSAGDMIGEVALAIEMGADATDIG
CCCCCCCEEECCCCCCHHHHEEECCCCCEEEEEEECCCCHHHHHHEEEEEEECCCHHHHC
RTIHPHPTLGETIGLAAEAGLGLCTDLPPLARR
CCCCCCCCCCHHHHHHHHCCCCCCCCCCHHCCC
>Mature Secondary Structure 
PETFDIRVPDLGDYADVPVIEIPVAPGQPVDKDQTLLVVESDKATLDIPSPAAGRLVEM
CCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCEEECCCCCHHHHHHH
LVGLGDTVSAGTLVARLAVEAPGARAEPLTAPVPAPRPAAPPPEPRAEAGCDLLVIGGGP
HHCCCCCCCHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEECCCC
GGYSAAFRAADLGQRVVLVERHATLGGVCLNVGCIPSKALLHVAAVTEEAVRLAAHGISF
CCHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
GAPTVDLEKLRTFKAGTVRRLTDGLAQMARQRKVEVVRGTARFIGPDTVSVTLHDGGTRD
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECHHHCCCCCEEEEEEECCCCCE
ITFTGAVVAAGSAPVALPMLPDDPRIVNSTGALELPAVPRRLLVIGGGIIGLEMATVYSA
EEEECEEEECCCCCEEEECCCCCCCEECCCCCEECCCCCCEEEEEECCCHHHHHHHHHHH
LGARVDVVERLPGLLEGVDRDLVGVWTKRNAHRFDRVLTGVEVVSAEARPDGIAVTFSGE
HCCCHHHHHHCCHHHHHCCCCCEEEECCCCHHHHHHHHHHHHEEECCCCCCCEEEEECCC
AEPGTYDLVLQAAGRRPNGATLGLDAAGVALDGGFVPVDAQMRTNVPHIFAIGDCVGQPM
CCCCEEEEEEEECCCCCCCCEEECCCCCEEECCCEEEECCHHHCCCCEEEEEHHHHCCCH
LAHKAVHQGHVAAEAAAGHKAGFDAAVIPSVAYTDPEIAWAGLTETAAKAAGRAVEVARF
HHHHHHHCCCHHHHHCCCCCCCCCCCCCCCEECCCCCEEECCCHHHHHHHCCCEEEEEEC
PWAASGRAIANGADYGLTKLLFDAQTGRVVGGAIVGPSAGDMIGEVALAIEMGADATDIG
CCCCCCCEEECCCCCCHHHHEEECCCCCEEEEEEECCCCHHHHHHEEEEEEECCCHHHHC
RTIHPHPTLGETIGLAAEAGLGLCTDLPPLARR
CCCCCCCCCCHHHHHHHHCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]