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The map label for this gene is proP [H]

Identifier: 170751963

GI number: 170751963

Start: 5921169

End: 5922851

Strand: Reverse

Name: proP [H]

Synonym: Mrad2831_5595

Alternate gene names: 170751963

Gene position: 5922851-5921169 (Counterclockwise)

Preceding gene: 170751964

Following gene: 170751958

Centisome position: 97.45

GC content: 66.55

Gene sequence:

>1683_bases
ATGCCTGTGGCAACCGCGATCCCGCGAGCGGGAGACGCTGCGAGGCCGATGACGTCGGCCGAGAAGCGGGTGATCTTCGC
ATCCTCGCTCGGCACCGTGTTCGAGTGGTACGATTTCTATCTCTACGGGTCGCTGGCGGCGATCATCGGCGCGCAATTCT
TCTCGGCCTACCCGCCGGCCACGCGCGACATCTTCGCTCTGCTGGCCTTCGCGGCCGGCTTCATCGTCCGGCCGTTCGGC
GCGCTGGTGTTCGGCCGTGTCGGCGATCTCGTCGGTCGCAAGTACACCTTCCTGGTCACGATCCTGATCATGGGTCTGTC
GACCTTCATCGTCGGCATCCTGCCCAACGCGGCGACCATCGGCATCGCGGCGCCGATCATCCTGATCGTCCTGCGCCTCG
CCCAGGGCCTCGCGCTGGGCGGCGAGTACGGCGGCGCGGCGACCTATGTGGCGGAGCACGCCCCCAATGGCCGGCGCGGC
TTCTACACGAGCTGGATCCAGACGACCGCGACGCTGGGCCTGTTCCTGTCGCTCCTGGTGATCCTGGCAACCCGGACCAT
CACGGGCGAGGCCGCCTTCGCCGAGTGGGGCTGGCGCATCCCGTTCTTGGTCTCGGTGCTGCTCCTCGGCATCTCGGTCT
GGATCCGTCTCCAGCTCTCCGAATCCCCGGCCTTCAAGAAGATGAAGGAGGAGGGCAAGACCTCGAAGGCGCCGCTCACG
GAGGCCTTCGGCGAGTGGCGGAACGCGAAGTTCGCGATCATCGCCCTGTTCGGACTCGTCGCCGGCCAGGGCGTGGTCTG
GTACACGGGCCAGTTCTACGCGCTGTTCTTCCTGCAATCGATCCTGAAGGTCGACGGCTACACGGCCAACCTGCTGATCG
CGTGGTCGCTGCTCCTCGGAACCGGCTTCTTCGTCGTGTTCGGCTGGCTGTCGGACAAGATCGGCCGCAAGGTCATCATC
CTGGCGGGCTGCGCGATCGCGGCGGCGACGTTCTTCCCGATCTTCAAGGGGATCACCTCCACGGCGAACCCGAAGCTCGA
GGCGGCGATCGAGAACGTGAAGGTGACGGTGACGGCCGATCCGGCCGCCTGCGGTACCCTGTTCAACCCGGTCGGAACGC
GGGTCTTCACCCAGCCCTGCGACCTCGCCCGCGACTTCCTGTCGAAGTCGTCGGTCCGCTACACGACGGTGCCCGGCGCG
GCCGGCCAGCCCACGGCGATCCGCGTCAACGACACCGAGATCCCGTACCCGCAGGGCGGCAACGTCGAGGCCAACAAGGC
CACGACCGCCGCGCTGCAGGCCGCGGGCTATCCGAAGGCCGGCGATCCCGAGATCGTCAAGATGTCGAACCCGTTCGACA
TCTTCCGGGCTCAGCCCGCGCAGGTGATCGGCCTGCTGTTCATCCTCGTGCTCTTCGTCACGATGGTCTACGGCCCGATC
GCCGCCATGCTGGTGGAGCTGTTCCCGACCCGGATCCGCTACACCTCGATGTCGCTGCCCTACCACATCGGCAACGGTTG
GTTCGGCGGCCTCCTGCCGGCGACCGCCTTCGCCATGGTGGCACAGACCGGCGACATCTATTTCGGCCTCTGGTACCCGA
TCGTCATCGCGGTCGCGACGGTGATCATCGGCGTGTTCTTCGTGCCGGAGACCAAGGATCGCGACATCTTCGCCGACACC
TGA

Upstream 100 bases:

>100_bases
TAGGTGATCTGCGCCTCGCGCAGAACCGCCCGGGAAAAGGGCCGGTGAGCGGGCGATCCGTGTCTCGGGCCGCCCATGTG
CGTCTGGGAGGACCTGTTTC

Downstream 100 bases:

>100_bases
CCGGCGCGAGCCGACGGACCCCGGTCAGACCCGCGGCAACGGCGCCCAGGCAGCACACGCTGCCTGGGCGTTCTCGTTCG
GGACAGCGCCCCGCGTCAGT

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MPVATAIPRAGDAARPMTSAEKRVIFASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATRDIFALLAFAAGFIVRPFG
ALVFGRVGDLVGRKYTFLVTILIMGLSTFIVGILPNAATIGIAAPIILIVLRLAQGLALGGEYGGAATYVAEHAPNGRRG
FYTSWIQTTATLGLFLSLLVILATRTITGEAAFAEWGWRIPFLVSVLLLGISVWIRLQLSESPAFKKMKEEGKTSKAPLT
EAFGEWRNAKFAIIALFGLVAGQGVVWYTGQFYALFFLQSILKVDGYTANLLIAWSLLLGTGFFVVFGWLSDKIGRKVII
LAGCAIAAATFFPIFKGITSTANPKLEAAIENVKVTVTADPAACGTLFNPVGTRVFTQPCDLARDFLSKSSVRYTTVPGA
AGQPTAIRVNDTEIPYPQGGNVEANKATTAALQAAGYPKAGDPEIVKMSNPFDIFRAQPAQVIGLLFILVLFVTMVYGPI
AAMLVELFPTRIRYTSMSLPYHIGNGWFGGLLPATAFAMVAQTGDIYFGLWYPIVIAVATVIIGVFFVPETKDRDIFADT

Sequences:

>Translated_560_residues
MPVATAIPRAGDAARPMTSAEKRVIFASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATRDIFALLAFAAGFIVRPFG
ALVFGRVGDLVGRKYTFLVTILIMGLSTFIVGILPNAATIGIAAPIILIVLRLAQGLALGGEYGGAATYVAEHAPNGRRG
FYTSWIQTTATLGLFLSLLVILATRTITGEAAFAEWGWRIPFLVSVLLLGISVWIRLQLSESPAFKKMKEEGKTSKAPLT
EAFGEWRNAKFAIIALFGLVAGQGVVWYTGQFYALFFLQSILKVDGYTANLLIAWSLLLGTGFFVVFGWLSDKIGRKVII
LAGCAIAAATFFPIFKGITSTANPKLEAAIENVKVTVTADPAACGTLFNPVGTRVFTQPCDLARDFLSKSSVRYTTVPGA
AGQPTAIRVNDTEIPYPQGGNVEANKATTAALQAAGYPKAGDPEIVKMSNPFDIFRAQPAQVIGLLFILVLFVTMVYGPI
AAMLVELFPTRIRYTSMSLPYHIGNGWFGGLLPATAFAMVAQTGDIYFGLWYPIVIAVATVIIGVFFVPETKDRDIFADT
>Mature_559_residues
PVATAIPRAGDAARPMTSAEKRVIFASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATRDIFALLAFAAGFIVRPFGA
LVFGRVGDLVGRKYTFLVTILIMGLSTFIVGILPNAATIGIAAPIILIVLRLAQGLALGGEYGGAATYVAEHAPNGRRGF
YTSWIQTTATLGLFLSLLVILATRTITGEAAFAEWGWRIPFLVSVLLLGISVWIRLQLSESPAFKKMKEEGKTSKAPLTE
AFGEWRNAKFAIIALFGLVAGQGVVWYTGQFYALFFLQSILKVDGYTANLLIAWSLLLGTGFFVVFGWLSDKIGRKVIIL
AGCAIAAATFFPIFKGITSTANPKLEAAIENVKVTVTADPAACGTLFNPVGTRVFTQPCDLARDFLSKSSVRYTTVPGAA
GQPTAIRVNDTEIPYPQGGNVEANKATTAALQAAGYPKAGDPEIVKMSNPFDIFRAQPAQVIGLLFILVLFVTMVYGPIA
AMLVELFPTRIRYTSMSLPYHIGNGWFGGLLPATAFAMVAQTGDIYFGLWYPIVIAVATVIIGVFFVPETKDRDIFADT

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=333, Percent_Identity=36.6366366366366, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1788292, Length=351, Percent_Identity=33.048433048433, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI1788942, Length=344, Percent_Identity=34.0116279069767, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1789941, Length=335, Percent_Identity=34.9253731343284, Blast_Score=147, Evalue=3e-36,
Organism=Escherichia coli, GI87082231, Length=242, Percent_Identity=28.099173553719, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082404, Length=222, Percent_Identity=28.3783783783784, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 60250; Mature: 60119

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVATAIPRAGDAARPMTSAEKRVIFASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPA
CCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TRDIFALLAFAAGFIVRPFGALVFGRVGDLVGRKYTFLVTILIMGLSTFIVGILPNAATI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
GIAAPIILIVLRLAQGLALGGEYGGAATYVAEHAPNGRRGFYTSWIQTTATLGLFLSLLV
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ILATRTITGEAAFAEWGWRIPFLVSVLLLGISVWIRLQLSESPAFKKMKEEGKTSKAPLT
HHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHCCCCCCCHH
EAFGEWRNAKFAIIALFGLVAGQGVVWYTGQFYALFFLQSILKVDGYTANLLIAWSLLLG
HHHHHHCCCCHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
TGFFVVFGWLSDKIGRKVIILAGCAIAAATFFPIFKGITSTANPKLEAAIENVKVTVTAD
HHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCEEEEEEEC
PAACGTLFNPVGTRVFTQPCDLARDFLSKSSVRYTTVPGAAGQPTAIRVNDTEIPYPQGG
CHHHHHHHCCCCCHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCEEEECCCCCCCCCCC
NVEANKATTAALQAAGYPKAGDPEIVKMSNPFDIFRAQPAQVIGLLFILVLFVTMVYGPI
CCCCCHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AAMLVELFPTRIRYTSMSLPYHIGNGWFGGLLPATAFAMVAQTGDIYFGLWYPIVIAVAT
HHHHHHHHHHHHEEEEECCCEEECCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
VIIGVFFVPETKDRDIFADT
HHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PVATAIPRAGDAARPMTSAEKRVIFASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPA
CCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TRDIFALLAFAAGFIVRPFGALVFGRVGDLVGRKYTFLVTILIMGLSTFIVGILPNAATI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
GIAAPIILIVLRLAQGLALGGEYGGAATYVAEHAPNGRRGFYTSWIQTTATLGLFLSLLV
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ILATRTITGEAAFAEWGWRIPFLVSVLLLGISVWIRLQLSESPAFKKMKEEGKTSKAPLT
HHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHCCCCCCCHH
EAFGEWRNAKFAIIALFGLVAGQGVVWYTGQFYALFFLQSILKVDGYTANLLIAWSLLLG
HHHHHHCCCCHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
TGFFVVFGWLSDKIGRKVIILAGCAIAAATFFPIFKGITSTANPKLEAAIENVKVTVTAD
HHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCEEEEEEEC
PAACGTLFNPVGTRVFTQPCDLARDFLSKSSVRYTTVPGAAGQPTAIRVNDTEIPYPQGG
CHHHHHHHCCCCCHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCEEEECCCCCCCCCCC
NVEANKATTAALQAAGYPKAGDPEIVKMSNPFDIFRAQPAQVIGLLFILVLFVTMVYGPI
CCCCCHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AAMLVELFPTRIRYTSMSLPYHIGNGWFGGLLPATAFAMVAQTGDIYFGLWYPIVIAVAT
HHHHHHHHHHHHEEEEECCCEEECCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
VIIGVFFVPETKDRDIFADT
HHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]