Click here to switch to the map view.

The map label for this gene is dapF [H]

Identifier: 170751705

GI number: 170751705

Start: 5645212

End: 5646189

Strand: Direct

Name: dapF [H]

Synonym: Mrad2831_5335

Alternate gene names: 170751705

Gene position: 5645212-5646189 (Clockwise)

Preceding gene: 170751699

Following gene: 170751706

Centisome position: 92.88

GC content: 73.21

Gene sequence:

>978_bases
GTGCGGCGCATCTGCCACGCCGCGGCACCCCTCGCGCCGGGCGGCGGCTTTTGCGATATAGCCCGCGCCTTCCTCCAACC
GCGGGACCGCATGAGCGCACTCGCCCACAGACGCTTCCTGAAGATGCACGGCGCCGGCAACGCGATCGTGGTGCTGGACC
TGCGCGGCTCCGCCGCGAAGGTGCGGCCCGAGGAGGCGCGGGCGATCGCGCAGGATCCCGGCTCGACCTTCGATCAGCTG
ATGGTCCTGCACGATCCCGTGAGCCCGGGCGCGGACGCGTTCATGCGCATCTACAACACCGACGGCTCGGAATCGGGCGC
CTGCGGCAACGGCACGCGCTGCGTCGCCTACGCGATGCTCGACGACCCGGCCATGGCCCGGCCGGCCGAGGGCGGCCGGC
TCGTCCTGGAGACCCGGGCGGGCCTGCTCGGGGTGCGCCGCGTCTCGGAGCGGGCCTTCACCGTGGATATGGGCCGGCCG
CGGCTCGCCTGGGACGAGATCCCCCTGTCCGAGCCGTTCCCCGACACCCGGCGGATCGAGCTGCAGATCGGCCCGATCGA
CGATCCGATCCTGCACTCGCCGGGCGCCGTGAACATGGGCAACCCGCACGCGGTGTTCTTCGTGGAGCAGGATCCGGACA
GCTTCGACCTCGCCCGGATCGGCCCGATGCTGGAGAACCACCCGCTGTTCCCGGAGCGCGCCAACATCTCGGTGGCGCAG
GTGACGGGCCGCGAGACGATCAAGCTCCGCGTCTGGGAGCGGGGGGCCGGGCTGACGCTCGCCTGCGGCACGGCCGCCTG
CGCCACCGTGGTGGCCGCCTCGCGCCTGCGGATGATCGGCCGGCAGGCCAGCGTCGCGCTGCCGGGCGGCGAGCTGTTCG
TCGAGTGGCGGGCCGACGACCACGTCCTGATGACCGGCCCGGTCGCGCTCGTCGCCGAGGGGACTCTGGCGCCGGAGCTG
TTCGACAGCGCGGCCTGA

Upstream 100 bases:

>100_bases
GCGCTCCGGTCGAGGCCCGCGGATGATCGCGCGGGCTCCCGCGGCGTAACCTAATGGCGATCGGGACGGCCCGCGAGGGC
GCAGCGGACGAGAGCCGCGG

Downstream 100 bases:

>100_bases
TGGCGGTCGAGACCCTCACCTTCGGCTGCCGCCTCAACACCGTCGAGTCCGAGGCCCTGCGGGTCCACGCGGCGGCGGAC
GGGCGCGACCGGGTGGTGGT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MRRICHAAAPLAPGGGFCDIARAFLQPRDRMSALAHRRFLKMHGAGNAIVVLDLRGSAAKVRPEEARAIAQDPGSTFDQL
MVLHDPVSPGADAFMRIYNTDGSESGACGNGTRCVAYAMLDDPAMARPAEGGRLVLETRAGLLGVRRVSERAFTVDMGRP
RLAWDEIPLSEPFPDTRRIELQIGPIDDPILHSPGAVNMGNPHAVFFVEQDPDSFDLARIGPMLENHPLFPERANISVAQ
VTGRETIKLRVWERGAGLTLACGTAACATVVAASRLRMIGRQASVALPGGELFVEWRADDHVLMTGPVALVAEGTLAPEL
FDSAA

Sequences:

>Translated_325_residues
MRRICHAAAPLAPGGGFCDIARAFLQPRDRMSALAHRRFLKMHGAGNAIVVLDLRGSAAKVRPEEARAIAQDPGSTFDQL
MVLHDPVSPGADAFMRIYNTDGSESGACGNGTRCVAYAMLDDPAMARPAEGGRLVLETRAGLLGVRRVSERAFTVDMGRP
RLAWDEIPLSEPFPDTRRIELQIGPIDDPILHSPGAVNMGNPHAVFFVEQDPDSFDLARIGPMLENHPLFPERANISVAQ
VTGRETIKLRVWERGAGLTLACGTAACATVVAASRLRMIGRQASVALPGGELFVEWRADDHVLMTGPVALVAEGTLAPEL
FDSAA
>Mature_325_residues
MRRICHAAAPLAPGGGFCDIARAFLQPRDRMSALAHRRFLKMHGAGNAIVVLDLRGSAAKVRPEEARAIAQDPGSTFDQL
MVLHDPVSPGADAFMRIYNTDGSESGACGNGTRCVAYAMLDDPAMARPAEGGRLVLETRAGLLGVRRVSERAFTVDMGRP
RLAWDEIPLSEPFPDTRRIELQIGPIDDPILHSPGAVNMGNPHAVFFVEQDPDSFDLARIGPMLENHPLFPERANISVAQ
VTGRETIKLRVWERGAGLTLACGTAACATVVAASRLRMIGRQASVALPGGELFVEWRADDHVLMTGPVALVAEGTLAPEL
FDSAA

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=286, Percent_Identity=37.0629370629371, Blast_Score=164, Evalue=7e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 34874; Mature: 34874

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRICHAAAPLAPGGGFCDIARAFLQPRDRMSALAHRRFLKMHGAGNAIVVLDLRGSAAK
CCCHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
VRPEEARAIAQDPGSTFDQLMVLHDPVSPGADAFMRIYNTDGSESGACGNGTRCVAYAML
CCHHHHHHHHCCCCCCHHHEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEE
DDPAMARPAEGGRLVLETRAGLLGVRRVSERAFTVDMGRPRLAWDEIPLSEPFPDTRRIE
CCCCCCCCCCCCEEEEEECCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEE
LQIGPIDDPILHSPGAVNMGNPHAVFFVEQDPDSFDLARIGPMLENHPLFPERANISVAQ
EEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHCCHHHCCCCCCCCCCEEEEE
VTGRETIKLRVWERGAGLTLACGTAACATVVAASRLRMIGRQASVALPGGELFVEWRADD
ECCCEEEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEECCC
HVLMTGPVALVAEGTLAPELFDSAA
EEEEECCEEEEECCCCCHHHHHCCC
>Mature Secondary Structure
MRRICHAAAPLAPGGGFCDIARAFLQPRDRMSALAHRRFLKMHGAGNAIVVLDLRGSAAK
CCCHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
VRPEEARAIAQDPGSTFDQLMVLHDPVSPGADAFMRIYNTDGSESGACGNGTRCVAYAML
CCHHHHHHHHCCCCCCHHHEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEE
DDPAMARPAEGGRLVLETRAGLLGVRRVSERAFTVDMGRPRLAWDEIPLSEPFPDTRRIE
CCCCCCCCCCCCEEEEEECCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEE
LQIGPIDDPILHSPGAVNMGNPHAVFFVEQDPDSFDLARIGPMLENHPLFPERANISVAQ
EEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHCCHHHCCCCCCCCCCEEEEE
VTGRETIKLRVWERGAGLTLACGTAACATVVAASRLRMIGRQASVALPGGELFVEWRADD
ECCCEEEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEECCC
HVLMTGPVALVAEGTLAPELFDSAA
EEEEECCEEEEECCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA