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The map label for this gene is pyrE [H]

Identifier: 170751690

GI number: 170751690

Start: 5628674

End: 5629375

Strand: Direct

Name: pyrE [H]

Synonym: Mrad2831_5320

Alternate gene names: 170751690

Gene position: 5628674-5629375 (Clockwise)

Preceding gene: 170751689

Following gene: 170751691

Centisome position: 92.61

GC content: 66.52

Gene sequence:

>702_bases
ATGACCAGTCCCTTCCTGCCCCTCGACCGGACAGTGATCGCCCGCGAGGCGGCCAAGATGTTCCTCGAGATCGGGGCCGT
CCTCTTCTACAAGGACGAGCCGTTCAAGTTCACCTCCGGCTGGGCGAGCCCGGTCTACACCGACAGCCGGAAGATCATCT
CGTTCCCGCGCCTGCGCTCGACGCTGATGGATTTCGCCACCGCCACGATCGTGCGCGAGATCGGCTACGAGAAGCTGACC
CACATCGCGGGCGGCGAGACCGCCGGCATCCCCTTCGCGGCCTGGATCGCCGACCGGATGATGCTGCCGATGCAGTACAT
CCGGAAGAAGCCCAAGGGCTTCGGCCGCAACGCCCAGATCGAGGGCGAGATCGTCGAGGGCGCCCGGACCCTGCTGGTCG
AGGACCTCGCCACCGACGGGCGCAGCAAGGTCAATTTCTGCAAGGCCCTGCGCGATTCCGGCGCCCAGGTCGATCACTGC
TTCGTGCTGTTCTACTACGACATCTTCCCCGACAGCGCCGCGCTGATGGAGGAGATCGGCATCAAGCTCCACTACCTCAC
CACGTGGTGGGACGTGCTGGCGGTGGCCAAGGAGATGGGCACCTTCGACCCGAAGACCCTGGCGGAGGTCGAGCGCTTCC
TCAACGCCCCGGCGGAGTGGTCGGCGGCCCACGGCGGCATCTCCGCCTTCGGCCAGGCCTGA

Upstream 100 bases:

>100_bases
GGGGGAGGAGGGCGGTCAGGCCCTTATTCAACGCGTTCTCCCCGGCCGGACCGGTCTACCGGACCGGCGGAGGACGTCTC
GAGCGGAGCGCGCACGCCGC

Downstream 100 bases:

>100_bases
GGAGAGGATCGCGATGGGCGTCGCCGAGCTGTTCCCCGCCGAGCGCCAGGAGGCGAACCCGGTCGACCGGATCACGATCC
GCCGGCCGGACGACTGGCAC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MTSPFLPLDRTVIAREAAKMFLEIGAVLFYKDEPFKFTSGWASPVYTDSRKIISFPRLRSTLMDFATATIVREIGYEKLT
HIAGGETAGIPFAAWIADRMMLPMQYIRKKPKGFGRNAQIEGEIVEGARTLLVEDLATDGRSKVNFCKALRDSGAQVDHC
FVLFYYDIFPDSAALMEEIGIKLHYLTTWWDVLAVAKEMGTFDPKTLAEVERFLNAPAEWSAAHGGISAFGQA

Sequences:

>Translated_233_residues
MTSPFLPLDRTVIAREAAKMFLEIGAVLFYKDEPFKFTSGWASPVYTDSRKIISFPRLRSTLMDFATATIVREIGYEKLT
HIAGGETAGIPFAAWIADRMMLPMQYIRKKPKGFGRNAQIEGEIVEGARTLLVEDLATDGRSKVNFCKALRDSGAQVDHC
FVLFYYDIFPDSAALMEEIGIKLHYLTTWWDVLAVAKEMGTFDPKTLAEVERFLNAPAEWSAAHGGISAFGQA
>Mature_232_residues
TSPFLPLDRTVIAREAAKMFLEIGAVLFYKDEPFKFTSGWASPVYTDSRKIISFPRLRSTLMDFATATIVREIGYEKLTH
IAGGETAGIPFAAWIADRMMLPMQYIRKKPKGFGRNAQIEGEIVEGARTLLVEDLATDGRSKVNFCKALRDSGAQVDHCF
VLFYYDIFPDSAALMEEIGIKLHYLTTWWDVLAVAKEMGTFDPKTLAEVERFLNAPAEWSAAHGGISAFGQA

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=205, Percent_Identity=30.2439024390244, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17508631, Length=121, Percent_Identity=32.2314049586777, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI17933654, Length=202, Percent_Identity=29.2079207920792, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 26017; Mature: 25886

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPFLPLDRTVIAREAAKMFLEIGAVLFYKDEPFKFTSGWASPVYTDSRKIISFPRLRS
CCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHH
TLMDFATATIVREIGYEKLTHIAGGETAGIPFAAWIADRMMLPMQYIRKKPKGFGRNAQI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
EGEIVEGARTLLVEDLATDGRSKVNFCKALRDSGAQVDHCFVLFYYDIFPDSAALMEEIG
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHEEEEEEEHHCCCHHHHHHHHC
IKLHYLTTWWDVLAVAKEMGTFDPKTLAEVERFLNAPAEWSAAHGGISAFGQA
CEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHCCCCCHHCCCC
>Mature Secondary Structure 
TSPFLPLDRTVIAREAAKMFLEIGAVLFYKDEPFKFTSGWASPVYTDSRKIISFPRLRS
CCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHH
TLMDFATATIVREIGYEKLTHIAGGETAGIPFAAWIADRMMLPMQYIRKKPKGFGRNAQI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
EGEIVEGARTLLVEDLATDGRSKVNFCKALRDSGAQVDHCFVLFYYDIFPDSAALMEEIG
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHEEEEEEEHHCCCHHHHHHHHC
IKLHYLTTWWDVLAVAKEMGTFDPKTLAEVERFLNAPAEWSAAHGGISAFGQA
CEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA