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The map label for this gene is emrB [H]

Identifier: 170751620

GI number: 170751620

Start: 5566021

End: 5567589

Strand: Reverse

Name: emrB [H]

Synonym: Mrad2831_5244

Alternate gene names: 170751620

Gene position: 5567589-5566021 (Counterclockwise)

Preceding gene: 170751621

Following gene: 170751619

Centisome position: 91.6

GC content: 63.29

Gene sequence:

>1569_bases
ATGAGCGACGCCTCGAACGGTCGAAAATCCGCCCACAACCCTTGGCTCGTCGCCATCGCGGTCACGCTGGCGGCGTTCAT
GGAGGTGCTCGACACCACCATCGTCAACGTGGCGCTGCCCTACATCGCCGGCTCGCTCGCGGCCTCGAACGACGAGGCGA
CCTATACCCTGACAGCCTACCTCGTCGCCAACGGTATTGTGCTGACCATCGCTGGCTGGCTGTCCGACACCATCGGACGC
AAGCGCTACTTCCTGATCTGCATCGGCATGTTCACGGTGTCGTCGTTCCTGTGCGGCATCTCGCAGAGCCTTGGGCAGAT
CATCGTCTTCCGCCTGATGCAGGGCTTCTTCGGCGGCGGCCTGCAGCCGAGCCAGCAGGCAATCATCCTCGACACCTTCC
CGCCGGCCAAGCGCGGGGCCGCCTTCGGCGTCACGGCCGTGGCGACCGTCGTCGCCCCCGTCCTCGGGCCGACGCTGGGC
GGCTACCTCGTCGAGCATTCGACCTGGCGCTGGATTTTCTTCATCAACGTGCCGGTCGGGATCTTCACCTTCTTCGTCAA
CGCATACCTCGTTCAGGACCCACCAGACGAGAAGAAGAAGCGCGCCCGCGGCCTCGACTACGTCGGCCTGTCGCTCATCG
TCATCGGCATCGGCTGCATGCAGGTCATGATCGACCGTGGCGAGGTCAACGGCTGGTTCAGCTCGTCCTTCATCCGGATA
ATGGCCGTCATCGCATTCCTGGGAATTTTCGGCGCGATCTGCTGGCTGCTGATCGCCGACGAGCCGGTCGTAGACCTCAA
CGTTTTCAAGGACCGGAACTTCTCCGTCGGCTGCCTGCTCATCGGTGCGGTCGGGGCACTGCTTTATGGCGGATCCGTCC
TTACTCCTAACTTCGCCCAGCAGGTGCTGGGCTACACGGCGCTTGATTCCGGCTTCATCTTGTCGCCGGGAGGCATCGCG
GTCATCTTCCTGATCCCCGTCGTCGCCGTAGCGATGAAGTTCGTCCAGACCCGCTACATCATCATGTTCGGGTTCGCCTT
CACGGGCGCCGCGATGTTCTATTCAAGCTTTCTTGCTCCCGACGTCGACTTTCGGACACTGGTCTGGATGCGCACGCTGC
AGACGGCGCCACTCGCCTTCCTGTTCGTGCCGATCTCCACGATCGCCTACCTGACCCTGCCGGACCGCTACCGCAGCGAC
GGATCCGCCTTGTTCTCCATGTTCCGCAACGTGTTCGGCTCCGTCGGGATCTCCCTCTCGACCGCCGCGGTGACGGAACG
AACGCAGGCCGACCAAGCCCATCTCTCGTCGTTCATGACGCCCCTACGCCACGGCTACAACCTGCTAATTCAGGAAAGCG
AGCACACGCTCCGGTCCCTTGGGCGGGCGGCGTCGTTGGTGCACCAGCAGGCTGTCGCGCATACCTACCAGACCTTCACG
AAGCAGGCCCAAGTGCTCGCCTACGGCAACGTGTACCAGTACGCGGCGGTCCTGGCGTTCCTCGTCGTACCCCTATGCCT
GTTGATCTCGGCCAAGACCGCGCAAGGCGAAGGCGGCGGGGGACACTGA

Upstream 100 bases:

>100_bases
TCGTTCAGCGCGTGCCGGTGAAGATCGTCATCGACGGCGGTCTGAAGAAGGACGAGCCGCTGCCGCTCGGCATCAGCGTC
GTACCGACGGTGATGCTCAA

Downstream 100 bases:

>100_bases
CATGACGCTCTCCCGATACCTCGGCCGACGCGGCCGAGCGATGATCACGGCCGGCCTGCTCGGCCTTGCCGGATGCACGG
TCGGGCCCGACTTCGCCCCG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MSDASNGRKSAHNPWLVAIAVTLAAFMEVLDTTIVNVALPYIAGSLAASNDEATYTLTAYLVANGIVLTIAGWLSDTIGR
KRYFLICIGMFTVSSFLCGISQSLGQIIVFRLMQGFFGGGLQPSQQAIILDTFPPAKRGAAFGVTAVATVVAPVLGPTLG
GYLVEHSTWRWIFFINVPVGIFTFFVNAYLVQDPPDEKKKRARGLDYVGLSLIVIGIGCMQVMIDRGEVNGWFSSSFIRI
MAVIAFLGIFGAICWLLIADEPVVDLNVFKDRNFSVGCLLIGAVGALLYGGSVLTPNFAQQVLGYTALDSGFILSPGGIA
VIFLIPVVAVAMKFVQTRYIIMFGFAFTGAAMFYSSFLAPDVDFRTLVWMRTLQTAPLAFLFVPISTIAYLTLPDRYRSD
GSALFSMFRNVFGSVGISLSTAAVTERTQADQAHLSSFMTPLRHGYNLLIQESEHTLRSLGRAASLVHQQAVAHTYQTFT
KQAQVLAYGNVYQYAAVLAFLVVPLCLLISAKTAQGEGGGGH

Sequences:

>Translated_522_residues
MSDASNGRKSAHNPWLVAIAVTLAAFMEVLDTTIVNVALPYIAGSLAASNDEATYTLTAYLVANGIVLTIAGWLSDTIGR
KRYFLICIGMFTVSSFLCGISQSLGQIIVFRLMQGFFGGGLQPSQQAIILDTFPPAKRGAAFGVTAVATVVAPVLGPTLG
GYLVEHSTWRWIFFINVPVGIFTFFVNAYLVQDPPDEKKKRARGLDYVGLSLIVIGIGCMQVMIDRGEVNGWFSSSFIRI
MAVIAFLGIFGAICWLLIADEPVVDLNVFKDRNFSVGCLLIGAVGALLYGGSVLTPNFAQQVLGYTALDSGFILSPGGIA
VIFLIPVVAVAMKFVQTRYIIMFGFAFTGAAMFYSSFLAPDVDFRTLVWMRTLQTAPLAFLFVPISTIAYLTLPDRYRSD
GSALFSMFRNVFGSVGISLSTAAVTERTQADQAHLSSFMTPLRHGYNLLIQESEHTLRSLGRAASLVHQQAVAHTYQTFT
KQAQVLAYGNVYQYAAVLAFLVVPLCLLISAKTAQGEGGGGH
>Mature_521_residues
SDASNGRKSAHNPWLVAIAVTLAAFMEVLDTTIVNVALPYIAGSLAASNDEATYTLTAYLVANGIVLTIAGWLSDTIGRK
RYFLICIGMFTVSSFLCGISQSLGQIIVFRLMQGFFGGGLQPSQQAIILDTFPPAKRGAAFGVTAVATVVAPVLGPTLGG
YLVEHSTWRWIFFINVPVGIFTFFVNAYLVQDPPDEKKKRARGLDYVGLSLIVIGIGCMQVMIDRGEVNGWFSSSFIRIM
AVIAFLGIFGAICWLLIADEPVVDLNVFKDRNFSVGCLLIGAVGALLYGGSVLTPNFAQQVLGYTALDSGFILSPGGIAV
IFLIPVVAVAMKFVQTRYIIMFGFAFTGAAMFYSSFLAPDVDFRTLVWMRTLQTAPLAFLFVPISTIAYLTLPDRYRSDG
SALFSMFRNVFGSVGISLSTAAVTERTQADQAHLSSFMTPLRHGYNLLIQESEHTLRSLGRAASLVHQQAVAHTYQTFTK
QAQVLAYGNVYQYAAVLAFLVVPLCLLISAKTAQGEGGGGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=482, Percent_Identity=36.0995850622407, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1788710, Length=507, Percent_Identity=31.3609467455621, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1790195, Length=441, Percent_Identity=29.4784580498866, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1788392, Length=418, Percent_Identity=27.511961722488, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87081983, Length=410, Percent_Identity=24.6341463414634, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1790146, Length=294, Percent_Identity=23.469387755102, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=160, Percent_Identity=28.125, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6322958, Length=185, Percent_Identity=29.7297297297297, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=29.3333333333333, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56338; Mature: 56207

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDASNGRKSAHNPWLVAIAVTLAAFMEVLDTTIVNVALPYIAGSLAASNDEATYTLTAY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHH
LVANGIVLTIAGWLSDTIGRKRYFLICIGMFTVSSFLCGISQSLGQIIVFRLMQGFFGGG
HHHCCHHEEEHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LQPSQQAIILDTFPPAKRGAAFGVTAVATVVAPVLGPTLGGYLVEHSTWRWIFFINVPVG
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHCCCCEECCCEEEEEEEECHHH
IFTFFVNAYLVQDPPDEKKKRARGLDYVGLSLIVIGIGCMQVMIDRGEVNGWFSSSFIRI
HHHHHHHHHEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
MAVIAFLGIFGAICWLLIADEPVVDLNVFKDRNFSVGCLLIGAVGALLYGGSVLTPNFAQ
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHH
QVLGYTALDSGFILSPGGIAVIFLIPVVAVAMKFVQTRYIIMFGFAFTGAAMFYSSFLAP
HHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCC
DVDFRTLVWMRTLQTAPLAFLFVPISTIAYLTLPDRYRSDGSALFSMFRNVFGSVGISLS
CCCHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCHHHCCCHHHHHHHHHHHHHHCCCEEE
TAAVTERTQADQAHLSSFMTPLRHGYNLLIQESEHTLRSLGRAASLVHQQAVAHTYQTFT
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQAQVLAYGNVYQYAAVLAFLVVPLCLLISAKTAQGEGGGGH
HHHHHEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SDASNGRKSAHNPWLVAIAVTLAAFMEVLDTTIVNVALPYIAGSLAASNDEATYTLTAY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHH
LVANGIVLTIAGWLSDTIGRKRYFLICIGMFTVSSFLCGISQSLGQIIVFRLMQGFFGGG
HHHCCHHEEEHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LQPSQQAIILDTFPPAKRGAAFGVTAVATVVAPVLGPTLGGYLVEHSTWRWIFFINVPVG
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHCCCCEECCCEEEEEEEECHHH
IFTFFVNAYLVQDPPDEKKKRARGLDYVGLSLIVIGIGCMQVMIDRGEVNGWFSSSFIRI
HHHHHHHHHEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
MAVIAFLGIFGAICWLLIADEPVVDLNVFKDRNFSVGCLLIGAVGALLYGGSVLTPNFAQ
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHH
QVLGYTALDSGFILSPGGIAVIFLIPVVAVAMKFVQTRYIIMFGFAFTGAAMFYSSFLAP
HHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCC
DVDFRTLVWMRTLQTAPLAFLFVPISTIAYLTLPDRYRSDGSALFSMFRNVFGSVGISLS
CCCHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCHHHCCCHHHHHHHHHHHHHHCCCEEE
TAAVTERTQADQAHLSSFMTPLRHGYNLLIQESEHTLRSLGRAASLVHQQAVAHTYQTFT
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQAQVLAYGNVYQYAAVLAFLVVPLCLLISAKTAQGEGGGGH
HHHHHEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]