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The map label for this gene is nuoE [H]

Identifier: 170751377

GI number: 170751377

Start: 5317284

End: 5317757

Strand: Direct

Name: nuoE [H]

Synonym: Mrad2831_4996

Alternate gene names: 170751377

Gene position: 5317284-5317757 (Clockwise)

Preceding gene: 170751376

Following gene: 170751378

Centisome position: 87.49

GC content: 71.94

Gene sequence:

>474_bases
ATGGCGCGTCACGAGCCCTGGAGCGCGGAGCGCGCGTCCGGGATCATCGCCGAGCACACCCATCTCGAAGGGGCCACGCT
GCCGATCCTGCACGCGCTGCAGGAAACCTTCGGCTACGTGGATTCCGGCGCCGTGCCGCTGATCGCGGACGCGCTGAACC
TGTCGCGCGCCGAGGTGCATGGCTGCATCACCTTCTACCACGACTTCCGCGCCCACCCCGCCGGCCGCCACGAGGTGAAG
CTGTGCCGGGCCGAGGCCTGCCAGGCCATGGGCTCCGACAAGCTGCACCGCGAGATCCTCGGCCGCCTCGGCTGCGGATG
GCACGAGACCACCGCCGACGGCAGCGCCACGGTCGAGCCGGTCTACTGCCTCGGCCTCTGCGCCAACGGTCCCGCCGCGC
TGGTGGACGGTGAGCCGGTCGCGCACCTGACCGCCGACGCCCTCGAAGCTGCCCTGACGGAGGTGCGCCAGTGA

Upstream 100 bases:

>100_bases
GACAAACGTCCTTTGTCGCCCGACCGGACAGCGCGATTGATGCCATTCGGCATTTCTGTTCCTCTTCGCATTAGAACAGG
AACAGACTGGGCTGGAAACC

Downstream 100 bases:

>100_bases
GCGTGACCCTCTACGTTCCGCGCGACGCCGTCGCCCTCGGCCTCGGCGCCAACAAGGTCGCCCGCGCCCTGTTCGCGGGC
GCCGAGCGCCGCGGCCTCGA

Product: NADH dehydrogenase (ubiquinone) 24 kDa subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MARHEPWSAERASGIIAEHTHLEGATLPILHALQETFGYVDSGAVPLIADALNLSRAEVHGCITFYHDFRAHPAGRHEVK
LCRAEACQAMGSDKLHREILGRLGCGWHETTADGSATVEPVYCLGLCANGPAALVDGEPVAHLTADALEAALTEVRQ

Sequences:

>Translated_157_residues
MARHEPWSAERASGIIAEHTHLEGATLPILHALQETFGYVDSGAVPLIADALNLSRAEVHGCITFYHDFRAHPAGRHEVK
LCRAEACQAMGSDKLHREILGRLGCGWHETTADGSATVEPVYCLGLCANGPAALVDGEPVAHLTADALEAALTEVRQ
>Mature_156_residues
ARHEPWSAERASGIIAEHTHLEGATLPILHALQETFGYVDSGAVPLIADALNLSRAEVHGCITFYHDFRAHPAGRHEVKL
CRAEACQAMGSDKLHREILGRLGCGWHETTADGSATVEPVYCLGLCANGPAALVDGEPVAHLTADALEAALTEVRQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=153, Percent_Identity=28.1045751633987, Blast_Score=78, Evalue=4e-15,
Organism=Escherichia coli, GI1788621, Length=132, Percent_Identity=32.5757575757576, Blast_Score=82, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17561328, Length=151, Percent_Identity=28.476821192053, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI18859877, Length=151, Percent_Identity=27.8145695364238, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355353, Length=151, Percent_Identity=26.4900662251656, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 16782; Mature: 16651

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARHEPWSAERASGIIAEHTHLEGATLPILHALQETFGYVDSGAVPLIADALNLSRAEVH
CCCCCCCCCHHHCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHH
GCITFYHDFRAHPAGRHEVKLCRAEACQAMGSDKLHREILGRLGCGWHETTADGSATVEP
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
VYCLGLCANGPAALVDGEPVAHLTADALEAALTEVRQ
EEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARHEPWSAERASGIIAEHTHLEGATLPILHALQETFGYVDSGAVPLIADALNLSRAEVH
CCCCCCCCHHHCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHH
GCITFYHDFRAHPAGRHEVKLCRAEACQAMGSDKLHREILGRLGCGWHETTADGSATVEP
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
VYCLGLCANGPAALVDGEPVAHLTADALEAALTEVRQ
EEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]