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The map label for this gene is nuoF2 [H]

Identifier: 170751378

GI number: 170751378

Start: 5317754

End: 5319313

Strand: Direct

Name: nuoF2 [H]

Synonym: Mrad2831_4997

Alternate gene names: 170751378

Gene position: 5317754-5319313 (Clockwise)

Preceding gene: 170751377

Following gene: 170751379

Centisome position: 87.49

GC content: 69.87

Gene sequence:

>1560_bases
GTGAGCGTGACCCTCTACGTTCCGCGCGACGCCGTCGCCCTCGGCCTCGGCGCCAACAAGGTCGCCCGCGCCCTGTTCGC
GGGCGCCGAGCGCCGCGGCCTCGACGTCACCATCGTCCGCACCGGCTCCCGCGGCCTGTTCTGGCTGGAGCCGATGGTCG
AAATCGGCACTCCCGAGGGGCGCGTCGCCTACGGACCGGTGACGGTCAAGGATGTCGATGCCCTGCTGGATGCCGGCCTG
ACCACGGGCGGCGACCACGCCCTGCGCCTCGGCGATCCTGAAAAAATCCCCTACCTCGCCCGGCAGCAGCGCCTGACCTT
CCACCGCTGCGGCGTGATCGACCCGGTCTCCGTGGACGATTACCGGGCCCATGGCGGCTACCGCGGCCTCGAGGCCGCCC
TGAAGCTCGATGCCGCGGGCATCGTCGCGGCGGTGCGCGAGTCCGGCCTGCGCGGCCGGGGCGGTGCCGGCTTCCCCGCC
GGCATCAAGTGGAACACTGTGATGCTCGCCGAGGCGGACCAGAAATACGTGGTCTGCAACGCCGACGAGGGCGATTCCGG
CACCTTCGCCGACCGGATGATGATGGAGGGCGATCCCTTCAACCTCATCGAGGGCATGACCATCGCGGGCGTCGCCACCG
GCGCCACCCGCGGCTACATCTACCTGCGCTCCGAGTACCCGCAGGCCTTCGCGACCCTGAAGGAGGCGATCGCCAACGGC
GTGACGGCGGGCGTGCTCGGCGACAACATCCTGGGCTCGGGCAAGAGCTTCCACCTGGACGTGCGCCTCGGCGCCGGGGC
CTATATCTGCGGCGAGGAGACCTCGCTGCTGGAGAGCCTGGAGGGCAAGCGCGGCATCGTGCGGGCCAAGCCGCCGATCC
CGGCGCTGAAGGGCTTCCTCGGCAAGCCGACGCTGGTCAACAACGTCATGACCTTCACGGCCGTGCCGTGGATCCTGGAG
AACGGCGCCAGGGCCTACGCCGATTACGGCATGGGCCGCTCGCTCGGCACCCTGCCGATCCAGCTCGCCGGCAATATCAA
GCACGGCGGCCTGATCGAGATGGCCTTCGGCATCACCCTCCGTCAGGTGATCGAGGATTTCGGCGGCGGCACCCGCTCAG
GTCGGCCGGTCCGGGCCGTGCAGGTCGGCGGGCCGCTCGGGGCCTACTTCCCCGACCATCTCCTCGACACGCCCCTCGAC
TACGAGGCGATGGCGGCCAAGAAGGGCCTCGTCGGCCACGGCGGCATCGTGGTGTTCGACGATACGGTCGATATGGCCGC
CCAGGCCCGCTTCGCCTTCGAGTTCTGCGCCACCGAGTCGTGCGGCAAGTGCACCCCCTGCCGCATCGGCGCGACCCGCG
GCGTCGAGACCATGGACAAGGTGATCGCCGGCATCCGGCCGGACGCGAACCTGAAGCTGGTCGAGGACCTCTGCGAGGTC
ATGACCGACGGGTCGCTCTGCGCCATGGGCGGGCTCACCCCGATGCCCGTCATGAGCGCGATCACCCATTTCCCTGAAGA
TTTTCGCCGTGCGGGCTCGCTGCCTGCGGCGGCTGAGTGA

Upstream 100 bases:

>100_bases
TCGGCCTCTGCGCCAACGGTCCCGCCGCGCTGGTGGACGGTGAGCCGGTCGCGCACCTGACCGCCGACGCCCTCGAAGCT
GCCCTGACGGAGGTGCGCCA

Downstream 100 bases:

>100_bases
GGCGGCGCGCATGGCCCTCATCAAAGAAATCGACTACGGCACGCCGATCCGCGTCAGCGCGCAGACGGTGACGCTGACGA
TCGACGGCATGTCGGTGACG

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MSVTLYVPRDAVALGLGANKVARALFAGAERRGLDVTIVRTGSRGLFWLEPMVEIGTPEGRVAYGPVTVKDVDALLDAGL
TTGGDHALRLGDPEKIPYLARQQRLTFHRCGVIDPVSVDDYRAHGGYRGLEAALKLDAAGIVAAVRESGLRGRGGAGFPA
GIKWNTVMLAEADQKYVVCNADEGDSGTFADRMMMEGDPFNLIEGMTIAGVATGATRGYIYLRSEYPQAFATLKEAIANG
VTAGVLGDNILGSGKSFHLDVRLGAGAYICGEETSLLESLEGKRGIVRAKPPIPALKGFLGKPTLVNNVMTFTAVPWILE
NGARAYADYGMGRSLGTLPIQLAGNIKHGGLIEMAFGITLRQVIEDFGGGTRSGRPVRAVQVGGPLGAYFPDHLLDTPLD
YEAMAAKKGLVGHGGIVVFDDTVDMAAQARFAFEFCATESCGKCTPCRIGATRGVETMDKVIAGIRPDANLKLVEDLCEV
MTDGSLCAMGGLTPMPVMSAITHFPEDFRRAGSLPAAAE

Sequences:

>Translated_519_residues
MSVTLYVPRDAVALGLGANKVARALFAGAERRGLDVTIVRTGSRGLFWLEPMVEIGTPEGRVAYGPVTVKDVDALLDAGL
TTGGDHALRLGDPEKIPYLARQQRLTFHRCGVIDPVSVDDYRAHGGYRGLEAALKLDAAGIVAAVRESGLRGRGGAGFPA
GIKWNTVMLAEADQKYVVCNADEGDSGTFADRMMMEGDPFNLIEGMTIAGVATGATRGYIYLRSEYPQAFATLKEAIANG
VTAGVLGDNILGSGKSFHLDVRLGAGAYICGEETSLLESLEGKRGIVRAKPPIPALKGFLGKPTLVNNVMTFTAVPWILE
NGARAYADYGMGRSLGTLPIQLAGNIKHGGLIEMAFGITLRQVIEDFGGGTRSGRPVRAVQVGGPLGAYFPDHLLDTPLD
YEAMAAKKGLVGHGGIVVFDDTVDMAAQARFAFEFCATESCGKCTPCRIGATRGVETMDKVIAGIRPDANLKLVEDLCEV
MTDGSLCAMGGLTPMPVMSAITHFPEDFRRAGSLPAAAE
>Mature_518_residues
SVTLYVPRDAVALGLGANKVARALFAGAERRGLDVTIVRTGSRGLFWLEPMVEIGTPEGRVAYGPVTVKDVDALLDAGLT
TGGDHALRLGDPEKIPYLARQQRLTFHRCGVIDPVSVDDYRAHGGYRGLEAALKLDAAGIVAAVRESGLRGRGGAGFPAG
IKWNTVMLAEADQKYVVCNADEGDSGTFADRMMMEGDPFNLIEGMTIAGVATGATRGYIYLRSEYPQAFATLKEAIANGV
TAGVLGDNILGSGKSFHLDVRLGAGAYICGEETSLLESLEGKRGIVRAKPPIPALKGFLGKPTLVNNVMTFTAVPWILEN
GARAYADYGMGRSLGTLPIQLAGNIKHGGLIEMAFGITLRQVIEDFGGGTRSGRPVRAVQVGGPLGAYFPDHLLDTPLDY
EAMAAKKGLVGHGGIVVFDDTVDMAAQARFAFEFCATESCGKCTPCRIGATRGVETMDKVIAGIRPDANLKLVEDLCEVM
TDGSLCAMGGLTPMPVMSAITHFPEDFRRAGSLPAAAE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=401, Percent_Identity=38.6533665835411, Blast_Score=248, Evalue=8e-66,
Organism=Homo sapiens, GI260656005, Length=401, Percent_Identity=38.6533665835411, Blast_Score=248, Evalue=1e-65,
Organism=Escherichia coli, GI1788620, Length=398, Percent_Identity=41.9597989949749, Blast_Score=310, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17531727, Length=401, Percent_Identity=38.4039900249377, Blast_Score=251, Evalue=7e-67,
Organism=Drosophila melanogaster, GI19920794, Length=406, Percent_Identity=36.9458128078818, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI45550452, Length=369, Percent_Identity=38.4823848238482, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24653819, Length=363, Percent_Identity=31.6804407713499, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24653817, Length=315, Percent_Identity=29.8412698412698, Blast_Score=145, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54787; Mature: 54655

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00626 RCC1_2 ; PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2 ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTLYVPRDAVALGLGANKVARALFAGAERRGLDVTIVRTGSRGLFWLEPMVEIGTPEG
CEEEEEECCCCEEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEECCHHHCCCCCC
RVAYGPVTVKDVDALLDAGLTTGGDHALRLGDPEKIPYLARQQRLTFHRCGVIDPVSVDD
EEEECCEEHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHCCHHHHCCCCCCCCCCC
YRAHGGYRGLEAALKLDAAGIVAAVRESGLRGRGGAGFPAGIKWNTVMLAEADQKYVVCN
HHCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEE
ADEGDSGTFADRMMMEGDPFNLIEGMTIAGVATGATRGYIYLRSEYPQAFATLKEAIANG
CCCCCCCCHHHHHEECCCCCHHHCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHCC
VTAGVLGDNILGSGKSFHLDVRLGAGAYICGEETSLLESLEGKRGIVRAKPPIPALKGFL
CEEECCCCCCCCCCCEEEEEEEECCCEEEECCHHHHHHHCCCCCCEEECCCCCHHHHHHC
GKPTLVNNVMTFTAVPWILENGARAYADYGMGRSLGTLPIQLAGNIKHGGLIEMAFGITL
CCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEECCCCCCCEEEHHHHHHH
RQVIEDFGGGTRSGRPVRAVQVGGPLGAYFPDHLLDTPLDYEAMAAKKGLVGHGGIVVFD
HHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCHHHHHHHCCCCCCCCEEEEE
DTVDMAAQARFAFEFCATESCGKCTPCRIGATRGVETMDKVIAGIRPDANLKLVEDLCEV
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
MTDGSLCAMGGLTPMPVMSAITHFPEDFRRAGSLPAAAE
HCCCCEEECCCCCHHHHHHHHHHCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
SVTLYVPRDAVALGLGANKVARALFAGAERRGLDVTIVRTGSRGLFWLEPMVEIGTPEG
EEEEEECCCCEEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEECCHHHCCCCCC
RVAYGPVTVKDVDALLDAGLTTGGDHALRLGDPEKIPYLARQQRLTFHRCGVIDPVSVDD
EEEECCEEHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHCCHHHHCCCCCCCCCCC
YRAHGGYRGLEAALKLDAAGIVAAVRESGLRGRGGAGFPAGIKWNTVMLAEADQKYVVCN
HHCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEE
ADEGDSGTFADRMMMEGDPFNLIEGMTIAGVATGATRGYIYLRSEYPQAFATLKEAIANG
CCCCCCCCHHHHHEECCCCCHHHCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHCC
VTAGVLGDNILGSGKSFHLDVRLGAGAYICGEETSLLESLEGKRGIVRAKPPIPALKGFL
CEEECCCCCCCCCCCEEEEEEEECCCEEEECCHHHHHHHCCCCCCEEECCCCCHHHHHHC
GKPTLVNNVMTFTAVPWILENGARAYADYGMGRSLGTLPIQLAGNIKHGGLIEMAFGITL
CCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEECCCCCCCEEEHHHHHHH
RQVIEDFGGGTRSGRPVRAVQVGGPLGAYFPDHLLDTPLDYEAMAAKKGLVGHGGIVVFD
HHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCHHHHHHHCCCCCCCCEEEEE
DTVDMAAQARFAFEFCATESCGKCTPCRIGATRGVETMDKVIAGIRPDANLKLVEDLCEV
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
MTDGSLCAMGGLTPMPVMSAITHFPEDFRRAGSLPAAAE
HCCCCEEECCCCCHHHHHHHHHHCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]