Click here to switch to the map view.

The map label for this gene is citZ [H]

Identifier: 170751250

GI number: 170751250

Start: 5198385

End: 5199542

Strand: Direct

Name: citZ [H]

Synonym: Mrad2831_4867

Alternate gene names: 170751250

Gene position: 5198385-5199542 (Clockwise)

Preceding gene: 170751235

Following gene: 170751252

Centisome position: 85.53

GC content: 79.02

Gene sequence:

>1158_bases
TTGATACATCTGATCAACATGACGGACTGGCTGGACCGCGCCGAAGCCCTCGACGCCCTCGGGATCCGGGCCCAGACGCT
CTACGCCTATGTCAGCCGCGGGCTGATCGAGGCGCGGCGCGCCCCGGGCGGGCGCCGCAGCCAGTACCGGGCCGCCGACG
TCGCGGCGCTCGCCGACCGGCGCGGCCGGAGCCGCGCCAGCGCGGCGATCGCCGCGGGGGCGATGGCCTGGGGCGAGCCG
TCGGTCGTCACCGCGATCAGCACCGTCCACCGGGGCAGCCTGATCTATCGCGGCCGCGACGCCGTGGCCTTCGCCCGCGA
CGCGAGCCTGGAGGCGACGGCGGACCTGCTGTGGGACAGCCCGGAGCCGACCCTCTTCGATGTGCCGGCCGGCGCCGCCG
CGACGCCGTTCCTGGCGCTGGCCGGCCTCCTGCAGGACGCCGAGGCCGCGCTCGGCCGCGGGCATCACCGCCTGTGCCGC
GACGCGCGCCGCGCCGTCGCGCATCTCGCTGGCGCCTGCGGGATCCCCGAAGGCCGGGCGCCCCTGCATCAGGGCCTCGC
GCGGCTCTGGTCCCTCGACGCCGCGACCGCCGACCGGGTCCGCCAGGCGCTGGTGCTGCTGGCCGACCACGAGCTGAACG
CCTCGACCTTCGCGACCCGCGTCGCCGCCTCCACCGGCGCGCCGATCGCGGCCTGCCTCATGGCCGGGCTGAGCGCCCTG
TCGGGCCCGCGCCACGGCGGCGCCGCCGCGGCGGTGCTGCGGCTCGTCGACGAGGCCGCCGCGACGGGCCCCCGGGCGGC
CGTCCGCGCCTGCCTGGACCGGGGCGGCGCGCTGCCGGGCTTCGGCCACCCGCTCTACCCGGAGGGCGACGTCCGCGCCG
CGGCCCTCGGCGCGGTCCTGCCCGCCGACCCCCTGCTGGACGCCGTCCGGGACTGCGCCGCGGAGGCGACGGGGGCGCGG
CCGAACATCGACTTCGCGCTGACGGCGCTCGTGCGGACGGCGGGCCTGCCGCGGAGCGCGCCGTTCACGATCTTCCTGCT
CGGCCGGAGCCTGGGCTGGGCGGCGCACGCCATGGAGCAGGGCCGCCAGGGCAGCCTGATCCGGCCGCGGGCCCGGTACG
AGGGGCGGCGGGGGCCGGCGGATCGCGCCGGCTCGTAG

Upstream 100 bases:

>100_bases
GGATCGGCGGCAGGGCGTCGTGGTCGGCATGTCGTTCGCGCATGGTCGCGGCCCTCGGGTGGTTCGCGCACGCTGCCGGC
CGTCGACGGACCGATCAATC

Downstream 100 bases:

>100_bases
CGGGAGGGCCGGCCCGCTCGGGTCGTTCCTGGTCTCAGGCGAACATGTCGGGCTGGGTCTCGGCGATGTGGTTGTACAGC
GTCTGGAAGTGCAGCCAGCC

Product: citrate synthase

Products: NA

Alternate protein names: Citrate synthase II [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MIHLINMTDWLDRAEALDALGIRAQTLYAYVSRGLIEARRAPGGRRSQYRAADVAALADRRGRSRASAAIAAGAMAWGEP
SVVTAISTVHRGSLIYRGRDAVAFARDASLEATADLLWDSPEPTLFDVPAGAAATPFLALAGLLQDAEAALGRGHHRLCR
DARRAVAHLAGACGIPEGRAPLHQGLARLWSLDAATADRVRQALVLLADHELNASTFATRVAASTGAPIAACLMAGLSAL
SGPRHGGAAAAVLRLVDEAAATGPRAAVRACLDRGGALPGFGHPLYPEGDVRAAALGAVLPADPLLDAVRDCAAEATGAR
PNIDFALTALVRTAGLPRSAPFTIFLLGRSLGWAAHAMEQGRQGSLIRPRARYEGRRGPADRAGS

Sequences:

>Translated_385_residues
MIHLINMTDWLDRAEALDALGIRAQTLYAYVSRGLIEARRAPGGRRSQYRAADVAALADRRGRSRASAAIAAGAMAWGEP
SVVTAISTVHRGSLIYRGRDAVAFARDASLEATADLLWDSPEPTLFDVPAGAAATPFLALAGLLQDAEAALGRGHHRLCR
DARRAVAHLAGACGIPEGRAPLHQGLARLWSLDAATADRVRQALVLLADHELNASTFATRVAASTGAPIAACLMAGLSAL
SGPRHGGAAAAVLRLVDEAAATGPRAAVRACLDRGGALPGFGHPLYPEGDVRAAALGAVLPADPLLDAVRDCAAEATGAR
PNIDFALTALVRTAGLPRSAPFTIFLLGRSLGWAAHAMEQGRQGSLIRPRARYEGRRGPADRAGS
>Mature_385_residues
MIHLINMTDWLDRAEALDALGIRAQTLYAYVSRGLIEARRAPGGRRSQYRAADVAALADRRGRSRASAAIAAGAMAWGEP
SVVTAISTVHRGSLIYRGRDAVAFARDASLEATADLLWDSPEPTLFDVPAGAAATPFLALAGLLQDAEAALGRGHHRLCR
DARRAVAHLAGACGIPEGRAPLHQGLARLWSLDAATADRVRQALVLLADHELNASTFATRVAASTGAPIAACLMAGLSAL
SGPRHGGAAAAVLRLVDEAAATGPRAAVRACLDRGGALPGFGHPLYPEGDVRAAALGAVLPADPLLDAVRDCAAEATGAR
PNIDFALTALVRTAGLPRSAPFTIFLLGRSLGWAAHAMEQGRQGSLIRPRARYEGRRGPADRAGS

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=196, Percent_Identity=33.1632653061224, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786939, Length=198, Percent_Identity=35.8585858585859, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 40010; Mature: 40010

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHLINMTDWLDRAEALDALGIRAQTLYAYVSRGLIEARRAPGGRRSQYRAADVAALADR
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
RGRSRASAAIAAGAMAWGEPSVVTAISTVHRGSLIYRGRDAVAFARDASLEATADLLWDS
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCEEEECCCCHHHHHHHHCCC
PEPTLFDVPAGAAATPFLALAGLLQDAEAALGRGHHRLCRDARRAVAHLAGACGIPEGRA
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
PLHQGLARLWSLDAATADRVRQALVLLADHELNASTFATRVAASTGAPIAACLMAGLSAL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
SGPRHGGAAAAVLRLVDEAAATGPRAAVRACLDRGGALPGFGHPLYPEGDVRAAALGAVL
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHC
PADPLLDAVRDCAAEATGARPNIDFALTALVRTAGLPRSAPFTIFLLGRSLGWAAHAMEQ
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHC
GRQGSLIRPRARYEGRRGPADRAGS
CCCCCCCCCHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MIHLINMTDWLDRAEALDALGIRAQTLYAYVSRGLIEARRAPGGRRSQYRAADVAALADR
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
RGRSRASAAIAAGAMAWGEPSVVTAISTVHRGSLIYRGRDAVAFARDASLEATADLLWDS
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCEEEECCCCHHHHHHHHCCC
PEPTLFDVPAGAAATPFLALAGLLQDAEAALGRGHHRLCRDARRAVAHLAGACGIPEGRA
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
PLHQGLARLWSLDAATADRVRQALVLLADHELNASTFATRVAASTGAPIAACLMAGLSAL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
SGPRHGGAAAAVLRLVDEAAATGPRAAVRACLDRGGALPGFGHPLYPEGDVRAAALGAVL
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHC
PADPLLDAVRDCAAEATGARPNIDFALTALVRTAGLPRSAPFTIFLLGRSLGWAAHAMEQ
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHC
GRQGSLIRPRARYEGRRGPADRAGS
CCCCCCCCCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377 [H]