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The map label for this gene is proP [H]

Identifier: 170751112

GI number: 170751112

Start: 5033812

End: 5035161

Strand: Direct

Name: proP [H]

Synonym: Mrad2831_4728

Alternate gene names: 170751112

Gene position: 5033812-5035161 (Clockwise)

Preceding gene: 170751111

Following gene: 170751113

Centisome position: 82.82

GC content: 67.56

Gene sequence:

>1350_bases
ATGCAGACCGCCGCCACCTTCGCATCCGATCCCCCCGCCCTCGCCAAACCCACAGGCCGGCAGACGGTGACCGCCGCCAT
GGCCTCGCTGTTCGGCTGGGGGCTCGATCTCTTCGACCTGTTCATCCTGCTTTACGTCGCGCCGGTCGTTGGCACGTTGT
TCTTCCCGGCGGACAAGCCGATGCTGTCGCTGGCCGGCGCCTATGCGTCCTTCGCGGTCACGCTGCTGATCCGGCCCCTC
GGCTCGGCCCTGTTCGGCTCCTATGCGGACCGCTTCGGCCGCCGTCGCGCCCTCATGGTGGCGGTGGTCGGCGTCGGCAT
ATCGACCGCGGTCTTCGGTCTCCTGCCGACGGTCGGCCAGATCGGATGGCTCGCGACTGCGGTCTTCCTTTTCTTCCGCC
TCGTCCAGGGCATCTTCGTCGGCGGCGTCGTCGCGGCATCCCACACGATCGGGACGGAATCCGTCCCGGAGCGGTGGCGG
GGGCTGATGTCCGGGGCGGTCGGCGGTGGCGGCTCGGCCATCGGCGGCCTGCTTGCCTCCCTGGTCTTCTATGTCGTCTC
GCTCATGGCGCCGGGAGAAGCCTTCGCCGAGTGGGGCTGGCGGCTGATGTTCTTCTCGGGTCTCCTGACCTCGGTGATCG
GCCTGATCCTCTTCCGCAATCTCGAGGAATCCCCGATCTTCAAGGAACTGCAGGCCCGGAAGGCGGCGCTCCGGGCAGGC
GCTCCGGCGGAGGCCTCACCGATCCGCTCCCTGTTCTCACCGTCCAACCGGGGAAGCTTCGCGGTCGCGACTCTCATCTC
CTTCGGTGGTGGCGCAGCCTACTACCTTACGTCCGGCTACCTGCCGACCCTCCTCAAGCTCGTGAACGGCGTGCCGAACG
CCACCGCCTCGATGATCCTGATCGGCGCCAACGTCGCGGCGGCGATCGGCGCCTGCGGCATGGGCGAACTGAGCCAGCAT
ATCGGGCGCAAACGGTCGTTCCTTCTGATGGGCGTCATCCGCCTGCTCGCTTTTCCCGCCCTGTTCCTGACCATGGCGAA
CACGACGAGCCTCGTTGGGGTCGCGGCCTGCGCCTTCCTGCTGGCGCTCATCGCCAACGGCAGCTACGGGCCGCTGCTGA
TCTTCCTCAACGAAAAATTCCCGACGGCGGTGCGGGCCACGGGGACGGGCCTGACCTGGAACATCGGCTTCGCGCTGGGC
GGCATGCTGCCGACGCTCGTCTCCCTTGTCGCCGACGGGCCGACGCAAATTCCGATGGTGCTGGCCGTCATCACGACCGG
CGTCACCCTGGTCTATCTTGTCGGTGCCTTCCTGACCGACGAGACGCAGGGCAATCTCGACCGGGCCTGA

Upstream 100 bases:

>100_bases
CAGGCGGGAAACGACGACGCACGCCCGAATGGCTCCCGCCCAGACCCAGAGCCCGAACCGGCCCCCCCGCGGCAACGACC
GCATCCCCCGGAGGAAATCT

Downstream 100 bases:

>100_bases
CGATACGGTTCTGCGCGGGCGGAGGCCTCCCATGACGAGTGATCCCCTCTGGACCTTCGCCTGCGCATTCTATGGCCGGC
CCGGCGTCGCCGCGGCCTGC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MQTAATFASDPPALAKPTGRQTVTAAMASLFGWGLDLFDLFILLYVAPVVGTLFFPADKPMLSLAGAYASFAVTLLIRPL
GSALFGSYADRFGRRRALMVAVVGVGISTAVFGLLPTVGQIGWLATAVFLFFRLVQGIFVGGVVAASHTIGTESVPERWR
GLMSGAVGGGGSAIGGLLASLVFYVVSLMAPGEAFAEWGWRLMFFSGLLTSVIGLILFRNLEESPIFKELQARKAALRAG
APAEASPIRSLFSPSNRGSFAVATLISFGGGAAYYLTSGYLPTLLKLVNGVPNATASMILIGANVAAAIGACGMGELSQH
IGRKRSFLLMGVIRLLAFPALFLTMANTTSLVGVAACAFLLALIANGSYGPLLIFLNEKFPTAVRATGTGLTWNIGFALG
GMLPTLVSLVADGPTQIPMVLAVITTGVTLVYLVGAFLTDETQGNLDRA

Sequences:

>Translated_449_residues
MQTAATFASDPPALAKPTGRQTVTAAMASLFGWGLDLFDLFILLYVAPVVGTLFFPADKPMLSLAGAYASFAVTLLIRPL
GSALFGSYADRFGRRRALMVAVVGVGISTAVFGLLPTVGQIGWLATAVFLFFRLVQGIFVGGVVAASHTIGTESVPERWR
GLMSGAVGGGGSAIGGLLASLVFYVVSLMAPGEAFAEWGWRLMFFSGLLTSVIGLILFRNLEESPIFKELQARKAALRAG
APAEASPIRSLFSPSNRGSFAVATLISFGGGAAYYLTSGYLPTLLKLVNGVPNATASMILIGANVAAAIGACGMGELSQH
IGRKRSFLLMGVIRLLAFPALFLTMANTTSLVGVAACAFLLALIANGSYGPLLIFLNEKFPTAVRATGTGLTWNIGFALG
GMLPTLVSLVADGPTQIPMVLAVITTGVTLVYLVGAFLTDETQGNLDRA
>Mature_449_residues
MQTAATFASDPPALAKPTGRQTVTAAMASLFGWGLDLFDLFILLYVAPVVGTLFFPADKPMLSLAGAYASFAVTLLIRPL
GSALFGSYADRFGRRRALMVAVVGVGISTAVFGLLPTVGQIGWLATAVFLFFRLVQGIFVGGVVAASHTIGTESVPERWR
GLMSGAVGGGGSAIGGLLASLVFYVVSLMAPGEAFAEWGWRLMFFSGLLTSVIGLILFRNLEESPIFKELQARKAALRAG
APAEASPIRSLFSPSNRGSFAVATLISFGGGAAYYLTSGYLPTLLKLVNGVPNATASMILIGANVAAAIGACGMGELSQH
IGRKRSFLLMGVIRLLAFPALFLTMANTTSLVGVAACAFLLALIANGSYGPLLIFLNEKFPTAVRATGTGLTWNIGFALG
GMLPTLVSLVADGPTQIPMVLAVITTGVTLVYLVGAFLTDETQGNLDRA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=426, Percent_Identity=30.9859154929577, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI1789941, Length=405, Percent_Identity=28.641975308642, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788292, Length=408, Percent_Identity=29.1666666666667, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788942, Length=404, Percent_Identity=28.7128712871287, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI87082404, Length=427, Percent_Identity=25.2927400468384, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI87082231, Length=251, Percent_Identity=28.2868525896414, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46678; Mature: 46678

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTAATFASDPPALAKPTGRQTVTAAMASLFGWGLDLFDLFILLYVAPVVGTLFFPADKP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCH
MLSLAGAYASFAVTLLIRPLGSALFGSYADRFGRRRALMVAVVGVGISTAVFGLLPTVGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IGWLATAVFLFFRLVQGIFVGGVVAASHTIGTESVPERWRGLMSGAVGGGGSAIGGLLAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
LVFYVVSLMAPGEAFAEWGWRLMFFSGLLTSVIGLILFRNLEESPIFKELQARKAALRAG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
APAEASPIRSLFSPSNRGSFAVATLISFGGGAAYYLTSGYLPTLLKLVNGVPNATASMIL
CCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEHHHCCCHHHHHHHHCCCCCCCHHEEE
IGANVAAAIGACGMGELSQHIGRKRSFLLMGVIRLLAFPALFLTMANTTSLVGVAACAFL
ECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALIANGSYGPLLIFLNEKFPTAVRATGTGLTWNIGFALGGMLPTLVSLVADGPTQIPMV
HHHHHCCCCCCEEEEECCCCCCHHEECCCCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHH
LAVITTGVTLVYLVGAFLTDETQGNLDRA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]