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The map label for this gene is fhlA [H]

Identifier: 170750929

GI number: 170750929

Start: 4820091

End: 4821914

Strand: Direct

Name: fhlA [H]

Synonym: Mrad2831_4540

Alternate gene names: 170750929

Gene position: 4820091-4821914 (Clockwise)

Preceding gene: 170750927

Following gene: 170750930

Centisome position: 79.31

GC content: 75.82

Gene sequence:

>1824_bases
ATGCTGGTCCTCGCGCCCGAGGAGGACCGGGTCGCGGACGCCAACCCGGCGGCGGCGCGCCTCCTCGGCTACGGCCGGCC
GGCGCTGCGCGGCGCGCGGGCCAGCGCCCTGTTCCCGGGCCAGCGCCCGGCCCTCACGGTCTTCACCGAGGCCGTCCTGG
CCAAGGGGCACTGGTGGACCCGGACGCTGGCGCCGCGCGACGCGGCCGGCCGCGACCTGCGGGTCGAGACGGCGGCCTAC
CCGCTCCCCGGCGACGCGATGCGCCTCCTCGTGACCCTGGCGGACCCGGACACGCTCCACCGCCGCCAGGTCGACGGCGA
GGCCGAGGCCCACATGCGCGCCGGCCTCTCCGAGTGGCAGCGCGTGGAGCGCATCTTCCGCGACATCGAGCGCGGCAACC
AGCTGATCCTGCGCGCGGCCGGGGAGGGGATCTACGGCGTCAACGCGGAGGGCGTGACCACCTTCGTCAACCCGGCCGCC
GCGCGCATGCTCGGCTGCGAGGCCGGCGACCTCGTCGGCCGGGACATGCACGCCGCCGTGCACCACACCCGCCCGGACGG
CAGCCACTACCCCCACCGCGACTGCCCAATCTACGCGGCCTTCCGGGACGGGATCGTCCACCAAGTCGATCACGAGGTCT
TCTGGCGCGCCGACGGGACCTCCTTCCCGGTCGAGTACACCTCGACGCCGATCCGCGACCGGGGGCGCCTGCTCGGCGCC
GTCATCGTGTTCCGCGACATCAGCCAGCGCCGGGAGGCCGAGGCCCGCCTGACGAGCGCGCTCGCGGAGGTCGACTCGCT
CCGGGAGCGCCTCGAGCTGGAGAACGCGTACCTGAAGGAGGAGATCCGGGCCGAGCGCCAGCACCAGGGCATCATCGGCC
GCTCGCCCGCCATCGCGGCGACGCTCCGGCAGGTCGACCGCGTCGCCGCCACCGACGCCACCGTGCTGATCACCGGCGAA
TCCGGCACCGGCAAGGAGCTGATCGCCCGGGCGATCCACGAGGCCAGCCGCCGCAGCGCCCGCCCGATGATCCGCGTCAA
CTGCGCGGCCATCCCCCGCGAGCTGTTCGAGAGCGAGTTCTTCGGCCACGCCAAGGGCAGCTTCACCGGGGCGCTGCGCG
ACCGTGTCGGCCGGTTCGAACTCGCCGACGGCGGCACGCTGTTCCTCGACGAGGTCGGCGAGATCCCCCTCGATCTCCAG
GGCAAGCTGCTGCGCGTCCTGCAGGAGCGCTCGTTCGAGCGGGTCGGCGAGGCGCGCACCCGGGCGGTGGACGTGCGCGT
GGTCGCGGCCACCAACCGGGACCTGCGCGCCGAGATCCGGGCGGGCCGCTTCCGGGAGGACCTCTACTTCCGCCTCAACG
TCTTCCCGATCCAGGCGCGGCCCCTGCGCGAGCGGCCCGAGGACATCCCGCCCATCGCCCAGCACCTGCTCCGGGTCGCC
GCGCGCCGCCTCACCGTCCCGGAGCCGCGCCTGACGGAGGGGGATGTCCGCCGTCTCTGCCGCTATTCCTGGCCCGGCAA
TGTCCGGGAACTGGAGAACGTGATCGAGCGGGCGGTGATCCTCTCCGCCCGGGGCCGCCTCCGCCTCGACCTGCCCGACG
GGGAGGCGGCCGGGCCCGCCGACGCGGCCGCCCCGGCCGCTCCGCCGGATGGCCGGCCCGCCACCGAGGCCGAGCGCCGG
GCGCGCGACCGCGCCGAGATCGTCGCGGCCCTGGCGCTGGCGGGCGGACGGATCTCCGGGCCGGGCGGCGCGGCCGCGCT
CCTCGGCCTGCGGCCGACGACGCTGGCCTCGCGCATCAAGGCCCACGGGATCCCGCGCGGCTGA

Upstream 100 bases:

>100_bases
CATCTCGTCGAAAACGAGATATCGTGAGCGGATGACGACATTTCGTGAGCCCGGCGTTGCGGGAACCTTCGGGCCGGTCT
TCGAGGCGAGTCCCGATCCC

Downstream 100 bases:

>100_bases
TCCGGCCACCCCTTGATCCGATCTTAAGGTCGCTGTGCCAACGATCGCGCCGGGCCTTCCCCGCGGAGGCGCAGCGTCTC
CGACCGATGCAGGACCTCTC

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MLVLAPEEDRVADANPAAARLLGYGRPALRGARASALFPGQRPALTVFTEAVLAKGHWWTRTLAPRDAAGRDLRVETAAY
PLPGDAMRLLVTLADPDTLHRRQVDGEAEAHMRAGLSEWQRVERIFRDIERGNQLILRAAGEGIYGVNAEGVTTFVNPAA
ARMLGCEAGDLVGRDMHAAVHHTRPDGSHYPHRDCPIYAAFRDGIVHQVDHEVFWRADGTSFPVEYTSTPIRDRGRLLGA
VIVFRDISQRREAEARLTSALAEVDSLRERLELENAYLKEEIRAERQHQGIIGRSPAIAATLRQVDRVAATDATVLITGE
SGTGKELIARAIHEASRRSARPMIRVNCAAIPRELFESEFFGHAKGSFTGALRDRVGRFELADGGTLFLDEVGEIPLDLQ
GKLLRVLQERSFERVGEARTRAVDVRVVAATNRDLRAEIRAGRFREDLYFRLNVFPIQARPLRERPEDIPPIAQHLLRVA
ARRLTVPEPRLTEGDVRRLCRYSWPGNVRELENVIERAVILSARGRLRLDLPDGEAAGPADAAAPAAPPDGRPATEAERR
ARDRAEIVAALALAGGRISGPGGAAALLGLRPTTLASRIKAHGIPRG

Sequences:

>Translated_607_residues
MLVLAPEEDRVADANPAAARLLGYGRPALRGARASALFPGQRPALTVFTEAVLAKGHWWTRTLAPRDAAGRDLRVETAAY
PLPGDAMRLLVTLADPDTLHRRQVDGEAEAHMRAGLSEWQRVERIFRDIERGNQLILRAAGEGIYGVNAEGVTTFVNPAA
ARMLGCEAGDLVGRDMHAAVHHTRPDGSHYPHRDCPIYAAFRDGIVHQVDHEVFWRADGTSFPVEYTSTPIRDRGRLLGA
VIVFRDISQRREAEARLTSALAEVDSLRERLELENAYLKEEIRAERQHQGIIGRSPAIAATLRQVDRVAATDATVLITGE
SGTGKELIARAIHEASRRSARPMIRVNCAAIPRELFESEFFGHAKGSFTGALRDRVGRFELADGGTLFLDEVGEIPLDLQ
GKLLRVLQERSFERVGEARTRAVDVRVVAATNRDLRAEIRAGRFREDLYFRLNVFPIQARPLRERPEDIPPIAQHLLRVA
ARRLTVPEPRLTEGDVRRLCRYSWPGNVRELENVIERAVILSARGRLRLDLPDGEAAGPADAAAPAAPPDGRPATEAERR
ARDRAEIVAALALAGGRISGPGGAAALLGLRPTTLASRIKAHGIPRG
>Mature_607_residues
MLVLAPEEDRVADANPAAARLLGYGRPALRGARASALFPGQRPALTVFTEAVLAKGHWWTRTLAPRDAAGRDLRVETAAY
PLPGDAMRLLVTLADPDTLHRRQVDGEAEAHMRAGLSEWQRVERIFRDIERGNQLILRAAGEGIYGVNAEGVTTFVNPAA
ARMLGCEAGDLVGRDMHAAVHHTRPDGSHYPHRDCPIYAAFRDGIVHQVDHEVFWRADGTSFPVEYTSTPIRDRGRLLGA
VIVFRDISQRREAEARLTSALAEVDSLRERLELENAYLKEEIRAERQHQGIIGRSPAIAATLRQVDRVAATDATVLITGE
SGTGKELIARAIHEASRRSARPMIRVNCAAIPRELFESEFFGHAKGSFTGALRDRVGRFELADGGTLFLDEVGEIPLDLQ
GKLLRVLQERSFERVGEARTRAVDVRVVAATNRDLRAEIRAGRFREDLYFRLNVFPIQARPLRERPEDIPPIAQHLLRVA
ARRLTVPEPRLTEGDVRRLCRYSWPGNVRELENVIERAVILSARGRLRLDLPDGEAAGPADAAAPAAPPDGRPATEAERR
ARDRAEIVAALALAGGRISGPGGAAALLGLRPTTLASRIKAHGIPRG

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=367, Percent_Identity=49.591280653951, Blast_Score=316, Evalue=3e-87,
Organism=Escherichia coli, GI87082117, Length=342, Percent_Identity=49.4152046783626, Blast_Score=312, Evalue=4e-86,
Organism=Escherichia coli, GI87082152, Length=303, Percent_Identity=47.8547854785479, Blast_Score=258, Evalue=1e-69,
Organism=Escherichia coli, GI1790437, Length=312, Percent_Identity=47.1153846153846, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1788550, Length=338, Percent_Identity=42.8994082840237, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1788905, Length=240, Percent_Identity=52.5, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1790299, Length=243, Percent_Identity=51.440329218107, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI1789233, Length=380, Percent_Identity=35.2631578947368, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87081872, Length=326, Percent_Identity=40.1840490797546, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1787583, Length=405, Percent_Identity=33.8271604938272, Blast_Score=202, Evalue=6e-53,
Organism=Escherichia coli, GI1786524, Length=246, Percent_Identity=46.3414634146341, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI87081858, Length=294, Percent_Identity=35.7142857142857, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1789828, Length=256, Percent_Identity=35.9375, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 66684; Mature: 66684

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVLAPEEDRVADANPAAARLLGYGRPALRGARASALFPGQRPALTVFTEAVLAKGHWWT
CEEECCCCCCCCCCCHHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEE
RTLAPRDAAGRDLRVETAAYPLPGDAMRLLVTLADPDTLHRRQVDGEAEAHMRAGLSEWQ
EECCCCCCCCCCEEEEECCCCCCCHHEEEEEEECCCCHHHHHHCCCHHHHHHHHCHHHHH
RVERIFRDIERGNQLILRAAGEGIYGVNAEGVTTFVNPAAARMLGCEAGDLVGRDMHAAV
HHHHHHHHHHCCCEEEEEECCCCEEECCCCCCEECCCHHHHHHHCCCCHHHCCCHHHHHH
HHTRPDGSHYPHRDCPIYAAFRDGIVHQVDHEVFWRADGTSFPVEYTSTPIRDRGRLLGA
HCCCCCCCCCCCCCCCEEHHHHCCHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
VIVFRDISQRREAEARLTSALAEVDSLRERLELENAYLKEEIRAERQHQGIIGRSPAIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
TLRQVDRVAATDATVLITGESGTGKELIARAIHEASRRSARPMIRVNCAAIPRELFESEF
HHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHH
FGHAKGSFTGALRDRVGRFELADGGTLFLDEVGEIPLDLQGKLLRVLQERSFERVGEART
CCCCCCCHHHHHHHCCCCEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RAVDVRVVAATNRDLRAEIRAGRFREDLYFRLNVFPIQARPLRERPEDIPPIAQHLLRVA
EEEEEEEEEECCCCHHHHHHHCCCCCCEEEEEEEEEECCCCHHCCCCCCCHHHHHHHHHH
ARRLTVPEPRLTEGDVRRLCRYSWPGNVRELENVIERAVILSARGRLRLDLPDGEAAGPA
HHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
DAAAPAAPPDGRPATEAERRARDRAEIVAALALAGGRISGPGGAAALLGLRPTTLASRIK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHH
AHGIPRG
HCCCCCC
>Mature Secondary Structure
MLVLAPEEDRVADANPAAARLLGYGRPALRGARASALFPGQRPALTVFTEAVLAKGHWWT
CEEECCCCCCCCCCCHHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEE
RTLAPRDAAGRDLRVETAAYPLPGDAMRLLVTLADPDTLHRRQVDGEAEAHMRAGLSEWQ
EECCCCCCCCCCEEEEECCCCCCCHHEEEEEEECCCCHHHHHHCCCHHHHHHHHCHHHHH
RVERIFRDIERGNQLILRAAGEGIYGVNAEGVTTFVNPAAARMLGCEAGDLVGRDMHAAV
HHHHHHHHHHCCCEEEEEECCCCEEECCCCCCEECCCHHHHHHHCCCCHHHCCCHHHHHH
HHTRPDGSHYPHRDCPIYAAFRDGIVHQVDHEVFWRADGTSFPVEYTSTPIRDRGRLLGA
HCCCCCCCCCCCCCCCEEHHHHCCHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
VIVFRDISQRREAEARLTSALAEVDSLRERLELENAYLKEEIRAERQHQGIIGRSPAIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
TLRQVDRVAATDATVLITGESGTGKELIARAIHEASRRSARPMIRVNCAAIPRELFESEF
HHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHH
FGHAKGSFTGALRDRVGRFELADGGTLFLDEVGEIPLDLQGKLLRVLQERSFERVGEART
CCCCCCCHHHHHHHCCCCEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RAVDVRVVAATNRDLRAEIRAGRFREDLYFRLNVFPIQARPLRERPEDIPPIAQHLLRVA
EEEEEEEEEECCCCHHHHHHHCCCCCCEEEEEEEEEECCCCHHCCCCCCCHHHHHHHHHH
ARRLTVPEPRLTEGDVRRLCRYSWPGNVRELENVIERAVILSARGRLRLDLPDGEAAGPA
HHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
DAAAPAAPPDGRPATEAERRARDRAEIVAALALAGGRISGPGGAAALLGLRPTTLASRIK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHH
AHGIPRG
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]