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The map label for this gene is mepA [H]

Identifier: 170750905

GI number: 170750905

Start: 4794997

End: 4795917

Strand: Reverse

Name: mepA [H]

Synonym: Mrad2831_4516

Alternate gene names: 170750905

Gene position: 4795917-4794997 (Counterclockwise)

Preceding gene: 170750907

Following gene: 170750903

Centisome position: 78.91

GC content: 72.53

Gene sequence:

>921_bases
ATGCCGATGCCGCGTTTCGCCCAGAGCCTCGCGCTCGCCGCCCTGCTCCTGCCCGCCGCCGCGCAGGCCCAGGATCGCGG
CAGCATCAACCCGAAGCCGCTGCCGCCCCTGGAGCATCCGGACGCGCCCTCGACGCCCGCCAAGGCCCTGTTCGGGCGCG
TCACCAGGCCGGCCGGAGGGCCGGCCCACATCTACGGCTTCTACGCCAAGGGCTGCTTCTCCGGCGGCGAGGCGCTGCCG
ATGGACGGGCCGAACTGGCAGGTCATGCGCCCGTCCCGCAACCGGATGTGGGGCACCCCCTACCTCATCGACTTCATCGA
GCGGCTGTCGCAGAAGGCCGCCCGGGTCGGCTGGCCCGGCCTGCTCGTGGGCGACATGGCCCAGCCCCGGGGCGGCCCGA
TGATCACCGGCCACGCCTCGCACCAGATCGGCATCGACGCCGACGTCTGGCTGACGCCGATGCCCGACCACCGCATGAGC
CGGGCGGAGCGCGAGGAGACCTCGGCGACCGACATGGTCCGCCGCGACCGCCTCGACATCGATCCCGAGGTCTGGACGCC
GACGCACCTGCAGCTGATCAAGATCACCGCCGAGCAGCCGGAGGTGGAGCGCATCTTCGTCAACGCGGCGATCAAGAAGG
CCCTCTGCCGCGACGCCGCGGGCAGCCGCTGGATGTCCAAGGTGCGCCCGATGTACGGGCACAATTACCACTACCATATC
CGGCTCGCCTGCCCGGCCGGCCAGGAGGATTGCCAGCCGCAGGGCGCGCCGCCGCCGGGCGACGGCTGCGACGACCTCAG
CTACTGGTTCTCGGACGCGGTGCTCCACCCCAAGCCCCCGAAGACGCCGCCGAAGCCCAAGCCCGCCATGACCATGGCGG
CGCTGCCCGCCGCCTGCCGGGCGGTGCTGAAGGCGCCGTAG

Upstream 100 bases:

>100_bases
CGCGCGGCGCGCGCCGTCTGCCCGAAAAACGCCCCGCCCCTGGGCCACGTTCCGGCGGGCGCCCGCGCCCGCCCTCTCCA
GCCGGACGAACAGCCCCCGC

Downstream 100 bases:

>100_bases
GCCGGCGCCCCGGGGCGGTCGCCGTCAGGGCGCGGCGGCCTCCGCGTAGGGCGTGCCGGCCAGCGCCGCCCGCAGGCCCG
CCGTCCCCCGATCGATGACT

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MPMPRFAQSLALAALLLPAAAQAQDRGSINPKPLPPLEHPDAPSTPAKALFGRVTRPAGGPAHIYGFYAKGCFSGGEALP
MDGPNWQVMRPSRNRMWGTPYLIDFIERLSQKAARVGWPGLLVGDMAQPRGGPMITGHASHQIGIDADVWLTPMPDHRMS
RAEREETSATDMVRRDRLDIDPEVWTPTHLQLIKITAEQPEVERIFVNAAIKKALCRDAAGSRWMSKVRPMYGHNYHYHI
RLACPAGQEDCQPQGAPPPGDGCDDLSYWFSDAVLHPKPPKTPPKPKPAMTMAALPAACRAVLKAP

Sequences:

>Translated_306_residues
MPMPRFAQSLALAALLLPAAAQAQDRGSINPKPLPPLEHPDAPSTPAKALFGRVTRPAGGPAHIYGFYAKGCFSGGEALP
MDGPNWQVMRPSRNRMWGTPYLIDFIERLSQKAARVGWPGLLVGDMAQPRGGPMITGHASHQIGIDADVWLTPMPDHRMS
RAEREETSATDMVRRDRLDIDPEVWTPTHLQLIKITAEQPEVERIFVNAAIKKALCRDAAGSRWMSKVRPMYGHNYHYHI
RLACPAGQEDCQPQGAPPPGDGCDDLSYWFSDAVLHPKPPKTPPKPKPAMTMAALPAACRAVLKAP
>Mature_305_residues
PMPRFAQSLALAALLLPAAAQAQDRGSINPKPLPPLEHPDAPSTPAKALFGRVTRPAGGPAHIYGFYAKGCFSGGEALPM
DGPNWQVMRPSRNRMWGTPYLIDFIERLSQKAARVGWPGLLVGDMAQPRGGPMITGHASHQIGIDADVWLTPMPDHRMSR
AEREETSATDMVRRDRLDIDPEVWTPTHLQLIKITAEQPEVERIFVNAAIKKALCRDAAGSRWMSKVRPMYGHNYHYHIR
LACPAGQEDCQPQGAPPPGDGCDDLSYWFSDAVLHPKPPKTPPKPKPAMTMAALPAACRAVLKAP

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=253, Percent_Identity=41.501976284585, Blast_Score=194, Evalue=7e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 33504; Mature: 33373

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMPRFAQSLALAALLLPAAAQAQDRGSINPKPLPPLEHPDAPSTPAKALFGRVTRPAGG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
PAHIYGFYAKGCFSGGEALPMDGPNWQVMRPSRNRMWGTPYLIDFIERLSQKAARVGWPG
CCEEEEEHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
LLVGDMAQPRGGPMITGHASHQIGIDADVWLTPMPDHRMSRAEREETSATDMVRRDRLDI
EEECCCCCCCCCCEEECCCCCEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCC
DPEVWTPTHLQLIKITAEQPEVERIFVNAAIKKALCRDAAGSRWMSKVRPMYGHNYHYHI
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEE
RLACPAGQEDCQPQGAPPPGDGCDDLSYWFSDAVLHPKPPKTPPKPKPAMTMAALPAACR
EEECCCCCHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AVLKAP
HHHCCC
>Mature Secondary Structure 
PMPRFAQSLALAALLLPAAAQAQDRGSINPKPLPPLEHPDAPSTPAKALFGRVTRPAGG
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
PAHIYGFYAKGCFSGGEALPMDGPNWQVMRPSRNRMWGTPYLIDFIERLSQKAARVGWPG
CCEEEEEHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
LLVGDMAQPRGGPMITGHASHQIGIDADVWLTPMPDHRMSRAEREETSATDMVRRDRLDI
EEECCCCCCCCCCEEECCCCCEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCC
DPEVWTPTHLQLIKITAEQPEVERIFVNAAIKKALCRDAAGSRWMSKVRPMYGHNYHYHI
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEE
RLACPAGQEDCQPQGAPPPGDGCDDLSYWFSDAVLHPKPPKTPPKPKPAMTMAALPAACR
EEECCCCCHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AVLKAP
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]