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The map label for this gene is yhjG [H]

Identifier: 170750903

GI number: 170750903

Start: 4792737

End: 4794263

Strand: Reverse

Name: yhjG [H]

Synonym: Mrad2831_4514

Alternate gene names: 170750903

Gene position: 4794263-4792737 (Counterclockwise)

Preceding gene: 170750905

Following gene: 170750902

Centisome position: 78.88

GC content: 76.88

Gene sequence:

>1527_bases
ATGGATCGGGCGATCGACGTCGTCGTGGCCGGAGCCGGGCCGGTCGGGCTCCTCACCGCCATCGAGCTGACCCTCGGGGG
CGCGCGCGTCCGGGTGCTGGAGCGCCTCACCGCGCCGAGCACCACCCTCAAGGCGCTCGGGGTCGGCCCCCTGGGCGTCG
AGGCGCTGCAGCGGCGGGGCATGGGCGCGGCGATCGAGGCCGCGGAGGCGCGGAGCCTGGCGGCGATGGACCGGGTGCCG
GGGCAGGCCGGCGGCGGCCTGCGCGGCGCGAAGTTCAGCGGCCATTTCGGCTTCCTGTTCATCCGCCGCGACCCGCAGGC
GGAGCCGGACCGGCGCATGCGCGCGGTCGATCAGCAGGGCCTCGAAGCCATGCTGATCGACCGCGCCGCGGCGCTCGGGG
TCGACCTGCGCCGCGGCTGCACGCTCACGGGCTTCACGGAGGCGGCCGACGGTGTCGAGGTCACCTGGACCGCGCCCGCG
GGCGACGGGCGGGTCCGCTGCGCCTATCTCGTCGGCTGCGACGGCGGCCGCAGCGCGGTCCGCAAGATGGCCGGCTTCGC
GTTTCCCGGCACGCCGCCGACGCTCACCATGTACCAGGCGATCGCCGATCTCGACGATCCCGCCGGCGCGCTGCCGGTCG
GCTGGCACCGCGGGCCGGGGGGCGTCTTCGGCCACGGGCCGTTCCCGCGCCGGCTGTTCATGCTGGATTTCAGCGGCCCG
CCGGCCGACCGGCAGGCGCCCGTCACGCGCGACGAGATCGAGACCGTGCTGCGCCGGGTCAGCGGCACGGATATCCGCGT
CGCGGGAGTCGAGGCCGCGAACCGGTGGGCCGACAACACGCGCCTCGTCGACACCTACCGCCGGGGCCGCGTGCTGCTGG
CCGGCGACGCGGCGCATGTCCACGCGCCCTTCGGCGGCCAGGGCCTGAGCCTCGGCCTGGGCGACGCCGCCAATCTCGGC
TGGAAGCTCGCCGCCGTGCTGCGCGGGGAGATGCCCGAGAGCCTGCTCGACACCTACACGGCCGAGCGGCGCCCGGTGGC
CGAGGCCGTCACGGCCAACACCCGCGCCCAGGCGGCGATCCTGCGGCCCGATCCGCAATCGGGCGCGCTGCGCGACCTGA
TGGCGCGGCTCCTGGAGAGCGACGAGGTCAGCCGGATCCTCGGCAGCCTGATGCGCGGCCTGAGCGCCCGGTACGATCTC
GGCTCGGCCGAGGACGCGGTCGGCCGGATGGCCGGCGACGGGCCGCTCGGCCCCGGCGGCGCGACGCTCTACGGGGCGAT
GCAGGACGGACGGGGCCTTCTCGTCGACGGCAGCGCCGACGGCGCGGCCTCCCGGATGGTCGCCGCGGCCACGGGGCGGG
TGACCTGCGTCGCGGTCCCGGAGGGGCCCTCGATGCTGGTCCGTCCCGACGGCTGCATCGCCTGGACGGGCGCGGCGCAC
GCCCTCGGCGGCCTGCGGGAGGCGGTAAGCCGCTGGTTCGGCCCGTCGCCGGGTGCGCCGCTCGGCGCGCCGGCCCCGAT
GTGCTAA

Upstream 100 bases:

>100_bases
CGTGGCGAGGCGCTTGCGGGACCGGCGGTCGGGGGGCGTTGACATGCGCCGCCACATACCACATCGATTTATTGACTGAG
TATATAAATGGATTGAGTCC

Downstream 100 bases:

>100_bases
CGGCCCCGGATGCCCCGCTGGCTCTTCATCCTCCTCGCGACCGCCCTCGCGCTCTCGGTCCTCGGCAGCGTGCTGATCGT
GCTGCGCAGCCCGAACAGCA

Product: FAD-binding monooxygenase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MDRAIDVVVAGAGPVGLLTAIELTLGGARVRVLERLTAPSTTLKALGVGPLGVEALQRRGMGAAIEAAEARSLAAMDRVP
GQAGGGLRGAKFSGHFGFLFIRRDPQAEPDRRMRAVDQQGLEAMLIDRAAALGVDLRRGCTLTGFTEAADGVEVTWTAPA
GDGRVRCAYLVGCDGGRSAVRKMAGFAFPGTPPTLTMYQAIADLDDPAGALPVGWHRGPGGVFGHGPFPRRLFMLDFSGP
PADRQAPVTRDEIETVLRRVSGTDIRVAGVEAANRWADNTRLVDTYRRGRVLLAGDAAHVHAPFGGQGLSLGLGDAANLG
WKLAAVLRGEMPESLLDTYTAERRPVAEAVTANTRAQAAILRPDPQSGALRDLMARLLESDEVSRILGSLMRGLSARYDL
GSAEDAVGRMAGDGPLGPGGATLYGAMQDGRGLLVDGSADGAASRMVAAATGRVTCVAVPEGPSMLVRPDGCIAWTGAAH
ALGGLREAVSRWFGPSPGAPLGAPAPMC

Sequences:

>Translated_508_residues
MDRAIDVVVAGAGPVGLLTAIELTLGGARVRVLERLTAPSTTLKALGVGPLGVEALQRRGMGAAIEAAEARSLAAMDRVP
GQAGGGLRGAKFSGHFGFLFIRRDPQAEPDRRMRAVDQQGLEAMLIDRAAALGVDLRRGCTLTGFTEAADGVEVTWTAPA
GDGRVRCAYLVGCDGGRSAVRKMAGFAFPGTPPTLTMYQAIADLDDPAGALPVGWHRGPGGVFGHGPFPRRLFMLDFSGP
PADRQAPVTRDEIETVLRRVSGTDIRVAGVEAANRWADNTRLVDTYRRGRVLLAGDAAHVHAPFGGQGLSLGLGDAANLG
WKLAAVLRGEMPESLLDTYTAERRPVAEAVTANTRAQAAILRPDPQSGALRDLMARLLESDEVSRILGSLMRGLSARYDL
GSAEDAVGRMAGDGPLGPGGATLYGAMQDGRGLLVDGSADGAASRMVAAATGRVTCVAVPEGPSMLVRPDGCIAWTGAAH
ALGGLREAVSRWFGPSPGAPLGAPAPMC
>Mature_508_residues
MDRAIDVVVAGAGPVGLLTAIELTLGGARVRVLERLTAPSTTLKALGVGPLGVEALQRRGMGAAIEAAEARSLAAMDRVP
GQAGGGLRGAKFSGHFGFLFIRRDPQAEPDRRMRAVDQQGLEAMLIDRAAALGVDLRRGCTLTGFTEAADGVEVTWTAPA
GDGRVRCAYLVGCDGGRSAVRKMAGFAFPGTPPTLTMYQAIADLDDPAGALPVGWHRGPGGVFGHGPFPRRLFMLDFSGP
PADRQAPVTRDEIETVLRRVSGTDIRVAGVEAANRWADNTRLVDTYRRGRVLLAGDAAHVHAPFGGQGLSLGLGDAANLG
WKLAAVLRGEMPESLLDTYTAERRPVAEAVTANTRAQAAILRPDPQSGALRDLMARLLESDEVSRILGSLMRGLSARYDL
GSAEDAVGRMAGDGPLGPGGATLYGAMQDGRGLLVDGSADGAASRMVAAATGRVTCVAVPEGPSMLVRPDGCIAWTGAAH
ALGGLREAVSRWFGPSPGAPLGAPAPMC

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=386, Percent_Identity=29.0155440414508, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 52643; Mature: 52643

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRAIDVVVAGAGPVGLLTAIELTLGGARVRVLERLTAPSTTLKALGVGPLGVEALQRRG
CCCEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHCC
MGAAIEAAEARSLAAMDRVPGQAGGGLRGAKFSGHFGFLFIRRDPQAEPDRRMRAVDQQG
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCEEEEEEECCCCCCHHHHHHHHHHHC
LEAMLIDRAAALGVDLRRGCTLTGFTEAADGVEVTWTAPAGDGRVRCAYLVGCDGGRSAV
CHHHHHHHHHHHCCHHHCCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCHHHH
RKMAGFAFPGTPPTLTMYQAIADLDDPAGALPVGWHRGPGGVFGHGPFPRRLFMLDFSGP
HHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCEEEEEECCCC
PADRQAPVTRDEIETVLRRVSGTDIRVAGVEAANRWADNTRLVDTYRRGRVLLAGDAAHV
CCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCEEEEHHHCCEEEEEECCEEE
HAPFGGQGLSLGLGDAANLGWKLAAVLRGEMPESLLDTYTAERRPVAEAVTANTRAQAAI
ECCCCCCCCEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCCEEEE
LRPDPQSGALRDLMARLLESDEVSRILGSLMRGLSARYDLGSAEDAVGRMAGDGPLGPGG
ECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCC
ATLYGAMQDGRGLLVDGSADGAASRMVAAATGRVTCVAVPEGPSMLVRPDGCIAWTGAAH
CEEEEEEECCCEEEEECCCCCHHHHEEEEECCCEEEEEECCCCCEEECCCCCEEECCHHH
ALGGLREAVSRWFGPSPGAPLGAPAPMC
HHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDRAIDVVVAGAGPVGLLTAIELTLGGARVRVLERLTAPSTTLKALGVGPLGVEALQRRG
CCCEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHCC
MGAAIEAAEARSLAAMDRVPGQAGGGLRGAKFSGHFGFLFIRRDPQAEPDRRMRAVDQQG
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCEEEEEEECCCCCCHHHHHHHHHHHC
LEAMLIDRAAALGVDLRRGCTLTGFTEAADGVEVTWTAPAGDGRVRCAYLVGCDGGRSAV
CHHHHHHHHHHHCCHHHCCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCHHHH
RKMAGFAFPGTPPTLTMYQAIADLDDPAGALPVGWHRGPGGVFGHGPFPRRLFMLDFSGP
HHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCEEEEEECCCC
PADRQAPVTRDEIETVLRRVSGTDIRVAGVEAANRWADNTRLVDTYRRGRVLLAGDAAHV
CCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCEEEEHHHCCEEEEEECCEEE
HAPFGGQGLSLGLGDAANLGWKLAAVLRGEMPESLLDTYTAERRPVAEAVTANTRAQAAI
ECCCCCCCCEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCCEEEE
LRPDPQSGALRDLMARLLESDEVSRILGSLMRGLSARYDLGSAEDAVGRMAGDGPLGPGG
ECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCC
ATLYGAMQDGRGLLVDGSADGAASRMVAAATGRVTCVAVPEGPSMLVRPDGCIAWTGAAH
CEEEEEEECCCEEEEECCCCCHHHHEEEEECCCEEEEEECCCCCEEECCCCCEEECCHHH
ALGGLREAVSRWFGPSPGAPLGAPAPMC
HHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]