Click here to switch to the map view.

The map label for this gene is trmB [H]

Identifier: 170750883

GI number: 170750883

Start: 4774992

End: 4775729

Strand: Direct

Name: trmB [H]

Synonym: Mrad2831_4494

Alternate gene names: 170750883

Gene position: 4774992-4775729 (Clockwise)

Preceding gene: 170750882

Following gene: 170750884

Centisome position: 78.56

GC content: 74.93

Gene sequence:

>738_bases
GTGGACACCGACACCCCTGGACGCCCCGAGCACCCGGCCGAGGAGCCGGAGCGCGCCTTCTTCGGGCGGCGCAAGGGCAA
GCGCCTGCGCGGCCAGCAGGAGCGCCGGCTCGCCGAATTGCTGCCGGCCCTGCGGGTCGACCTGCCGCCGGGCGAGGCGA
TCCTCGATCCGCGCGCGCTGTTCCCGGCCATGCCGGTGCCGCCCGAGGCGCTCTGGCTGGAGATCGGCTTCGGCGGCGGC
GAGCACCTCGCCGCCCAGGCGGCCGCGCGGCCGGATATCGGCATCATCGGTGCCGAGCCCTTCGTCAACGGCGTGGTCAA
GCTGCTGGCGGCCGTCGAGGCGCAGGGGCTGCGCAACGTCCGCATCCGCGACGAGGACGTGACCGCCCTGCTGGCGCGCC
TGCCGGATGCCTGCCTCGACCGGGTCTACCTGCTCTATCCCGATCCCTGGCCCAAGCGGCGGCAGCGCAAGCGCCGGTTC
GTCTCCGACGCCTCCCTCGCCGAGATCGGCCGGGTCCTGAAGGACGGGGGCCTGTTCCGCTTCGCCAGCGACATCGACGA
CTATGCCGGCTGGACGCTGGTCCGGGCCGCGCGGTGCCCGGTCCTCGCCTGGACGGCGCGGGACGCGCGGGACTGGACGC
GACCTTTCCCGGGCTGGCCCGGCACGCGCTACGAGGCCAAGGCGCTGGAAGCGGGCCGCCGGCCGACCTACCTGGAATTC
GTCCGCCGCCCCCGGTGA

Upstream 100 bases:

>100_bases
GCGGCGCGTCGGGTGCGGTGTCCCGGTTGGCGTCCCGCCGACGCTCCGCGTGGCCCGCCGGGACGCGGGCCGCGTCGCAT
CTCCGAGGTAGACTGAGCCG

Downstream 100 bases:

>100_bases
CGGCCCGCGCGGGCGGGTCGCGCGGGTGCCCGGTCGCCGCAGGCGGGGCGGAACGCCTTGCCACGGTCACGCTCGGGCGC
CATCCTCAGGGCCCGCCGCG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MDTDTPGRPEHPAEEPERAFFGRRKGKRLRGQQERRLAELLPALRVDLPPGEAILDPRALFPAMPVPPEALWLEIGFGGG
EHLAAQAAARPDIGIIGAEPFVNGVVKLLAAVEAQGLRNVRIRDEDVTALLARLPDACLDRVYLLYPDPWPKRRQRKRRF
VSDASLAEIGRVLKDGGLFRFASDIDDYAGWTLVRAARCPVLAWTARDARDWTRPFPGWPGTRYEAKALEAGRRPTYLEF
VRRPR

Sequences:

>Translated_245_residues
MDTDTPGRPEHPAEEPERAFFGRRKGKRLRGQQERRLAELLPALRVDLPPGEAILDPRALFPAMPVPPEALWLEIGFGGG
EHLAAQAAARPDIGIIGAEPFVNGVVKLLAAVEAQGLRNVRIRDEDVTALLARLPDACLDRVYLLYPDPWPKRRQRKRRF
VSDASLAEIGRVLKDGGLFRFASDIDDYAGWTLVRAARCPVLAWTARDARDWTRPFPGWPGTRYEAKALEAGRRPTYLEF
VRRPR
>Mature_245_residues
MDTDTPGRPEHPAEEPERAFFGRRKGKRLRGQQERRLAELLPALRVDLPPGEAILDPRALFPAMPVPPEALWLEIGFGGG
EHLAAQAAARPDIGIIGAEPFVNGVVKLLAAVEAQGLRNVRIRDEDVTALLARLPDACLDRVYLLYPDPWPKRRQRKRRF
VSDASLAEIGRVLKDGGLFRFASDIDDYAGWTLVRAARCPVLAWTARDARDWTRPFPGWPGTRYEAKALEAGRRPTYLEF
VRRPR

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=174, Percent_Identity=36.7816091954023, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 27559; Mature: 27559

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTDTPGRPEHPAEEPERAFFGRRKGKRLRGQQERRLAELLPALRVDLPPGEAILDPRAL
CCCCCCCCCCCCCCCHHHHHHHHHCCHHCCCHHHHHHHHHHHHHCCCCCCCCHHCCCHHH
FPAMPVPPEALWLEIGFGGGEHLAAQAAARPDIGIIGAEPFVNGVVKLLAAVEAQGLRNV
CCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCC
RIRDEDVTALLARLPDACLDRVYLLYPDPWPKRRQRKRRFVSDASLAEIGRVLKDGGLFR
EECCHHHHHHHHHCCHHHHCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEE
FASDIDDYAGWTLVRAARCPVLAWTARDARDWTRPFPGWPGTRYEAKALEAGRRPTYLEF
EHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCCCCHHHHHHCCCCCHHHHH
VRRPR
HCCCC
>Mature Secondary Structure
MDTDTPGRPEHPAEEPERAFFGRRKGKRLRGQQERRLAELLPALRVDLPPGEAILDPRAL
CCCCCCCCCCCCCCCHHHHHHHHHCCHHCCCHHHHHHHHHHHHHCCCCCCCCHHCCCHHH
FPAMPVPPEALWLEIGFGGGEHLAAQAAARPDIGIIGAEPFVNGVVKLLAAVEAQGLRNV
CCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCC
RIRDEDVTALLARLPDACLDRVYLLYPDPWPKRRQRKRRFVSDASLAEIGRVLKDGGLFR
EECCHHHHHHHHHCCHHHHCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEE
FASDIDDYAGWTLVRAARCPVLAWTARDARDWTRPFPGWPGTRYEAKALEAGRRPTYLEF
EHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCCCCHHHHHHCCCCCHHHHH
VRRPR
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]