| Definition | Methylobacterium radiotolerans JCM 2831 chromosome, complete genome. |
|---|---|
| Accession | NC_010505 |
| Length | 6,077,833 |
Click here to switch to the map view.
The map label for this gene is idnO [C]
Identifier: 170750833
GI number: 170750833
Start: 4727603
End: 4728397
Strand: Direct
Name: idnO [C]
Synonym: Mrad2831_4444
Alternate gene names: 170750833
Gene position: 4727603-4728397 (Clockwise)
Preceding gene: 170750832
Following gene: 170750834
Centisome position: 77.78
GC content: 77.61
Gene sequence:
>795_bases ATGACACAGGCTCACGGCAAGGCCCTGGTGACGGGCGCGTCCTCGGGGATCGGCGCGGAGTACGCGGCGCAGCTGGCGGC GCGGGGGCACGACCTCGTCCTCGTGGCCCGCGACGCGGCCCGGCTGGAGGCCCTCGCGGCGCGCCTCGGCGGGGAGACGG GCGTGGCGGTCGAGGTTCTGCCCGCCGACCTGACCCGGCCGGCCGACCTCGCCGCGGTCGCCGCGCGCCTCGCCGCCGAC GCGGCGCTGCGCCTGCTGGTGAACTGCGCCGGCCTCGGGCCGATGGGGCCGGTCCTCCCCGGGCCGGCGTCGGGCTACGA CCCCATGGTCGCCCTCAACGTGACGGCGCTCCAGCACCTGACGCTGGCGGCGGCCCCAGCGTTCGCGGAGCGCGGCGGCG GCACGATCGTGAACGTGTCGTCGGTGGTCGCCCTGCTGCCCGAGCGCTTCAACGCGGTCTACGCGGCCAGCAAGGCCTAC GTGCTGGCGCTCACCCAGGGGCTCGCCGCCGAGCTCGCGCCCCGGGGCGTGCGCCTGCAGGCGGTGCTGCCCGGCATCAC CCGCACCGAGCTGTTCGCGCGGGCGGGCGCCGATCTCGACCGGATCCCCGCCGCGATGATCATGGAGGCGCGGGATCTCG TGGCCGCGGCCCTCGCGGGGCTCGACGCGGGCGAGCCGGTCACGATCCCGTCCCTGCCCGAGATCGACGACTGGGCAGCC TTCGAGGCCGCCCGCCTGCGCCTCGGCCCGAACCTCTCCCGCAGCGCGCCGGCCGCGCGCTACGGCCTCGGCTGA
Upstream 100 bases:
>100_bases GTCGCGGCGCTGACCGGCCAGCGGATCCGCGCCCTGCCGATGGCCAACACCAAGCTCGGCGCCGCCTGAGCGCCCGACAA CTGACCGCGGGACGACCACG
Downstream 100 bases:
>100_bases GCGGCGTCCGGGGCGCGCGGCCCGCCCTGGCCCGGGCGCACCGGACCCGCTAGGAACCGCCCGTCCCGGAGAGCGAGGCG TCGGCGTCCCGCGGCGGCCC
Product: short-chain dehydrogenase/reductase SDR
Products: D-Gluconate; Nicotinamide adenine dinucleotide phosphate [C]
Alternate protein names: NA
Number of amino acids: Translated: 264; Mature: 263
Protein sequence:
>264_residues MTQAHGKALVTGASSGIGAEYAAQLAARGHDLVLVARDAARLEALAARLGGETGVAVEVLPADLTRPADLAAVAARLAAD AALRLLVNCAGLGPMGPVLPGPASGYDPMVALNVTALQHLTLAAAPAFAERGGGTIVNVSSVVALLPERFNAVYAASKAY VLALTQGLAAELAPRGVRLQAVLPGITRTELFARAGADLDRIPAAMIMEARDLVAAALAGLDAGEPVTIPSLPEIDDWAA FEAARLRLGPNLSRSAPAARYGLG
Sequences:
>Translated_264_residues MTQAHGKALVTGASSGIGAEYAAQLAARGHDLVLVARDAARLEALAARLGGETGVAVEVLPADLTRPADLAAVAARLAAD AALRLLVNCAGLGPMGPVLPGPASGYDPMVALNVTALQHLTLAAAPAFAERGGGTIVNVSSVVALLPERFNAVYAASKAY VLALTQGLAAELAPRGVRLQAVLPGITRTELFARAGADLDRIPAAMIMEARDLVAAALAGLDAGEPVTIPSLPEIDDWAA FEAARLRLGPNLSRSAPAARYGLG >Mature_263_residues TQAHGKALVTGASSGIGAEYAAQLAARGHDLVLVARDAARLEALAARLGGETGVAVEVLPADLTRPADLAAVAARLAADA ALRLLVNCAGLGPMGPVLPGPASGYDPMVALNVTALQHLTLAAAPAFAERGGGTIVNVSSVVALLPERFNAVYAASKAYV LALTQGLAAELAPRGVRLQAVLPGITRTELFARAGADLDRIPAAMIMEARDLVAAALAGLDAGEPVTIPSLPEIDDWAAF EAARLRLGPNLSRSAPAARYGLG
Specific function: Catalyzes A Reversible Reduction Of 5-Ketoglutanate To Form D-Gluconate. Dependent On Nadp, Almost Inactive With NAD. [C]
COG id: COG0300
COG function: function code R; Short-chain dehydrogenases of various substrate specificities
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI4557649, Length=181, Percent_Identity=32.0441988950276, Blast_Score=97, Evalue=2e-20, Organism=Homo sapiens, GI7705855, Length=198, Percent_Identity=28.2828282828283, Blast_Score=89, Evalue=3e-18, Organism=Homo sapiens, GI32483357, Length=195, Percent_Identity=33.8461538461538, Blast_Score=88, Evalue=1e-17, Organism=Homo sapiens, GI109715829, Length=211, Percent_Identity=35.0710900473934, Blast_Score=84, Evalue=1e-16, Organism=Homo sapiens, GI226371731, Length=195, Percent_Identity=27.1794871794872, Blast_Score=80, Evalue=1e-15, Organism=Homo sapiens, GI5031737, Length=186, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=2e-15, Organism=Homo sapiens, GI33667109, Length=186, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=3e-15, Organism=Homo sapiens, GI7706318, Length=183, Percent_Identity=33.879781420765, Blast_Score=79, Evalue=4e-15, Organism=Homo sapiens, GI126723191, Length=185, Percent_Identity=31.8918918918919, Blast_Score=73, Evalue=2e-13, Organism=Homo sapiens, GI7705925, Length=220, Percent_Identity=30.4545454545455, Blast_Score=72, Evalue=4e-13, Organism=Homo sapiens, GI15277342, Length=242, Percent_Identity=30.9917355371901, Blast_Score=72, Evalue=5e-13, Organism=Homo sapiens, GI40254992, Length=183, Percent_Identity=28.4153005464481, Blast_Score=70, Evalue=1e-12, Organism=Homo sapiens, GI7657478, Length=186, Percent_Identity=31.7204301075269, Blast_Score=70, Evalue=3e-12, Organism=Homo sapiens, GI20149619, Length=189, Percent_Identity=30.1587301587302, Blast_Score=67, Evalue=1e-11, Organism=Homo sapiens, GI10190704, Length=191, Percent_Identity=27.7486910994764, Blast_Score=65, Evalue=8e-11, Organism=Homo sapiens, GI66933014, Length=137, Percent_Identity=30.6569343065693, Blast_Score=65, Evalue=8e-11, Organism=Escherichia coli, GI1790717, Length=218, Percent_Identity=27.9816513761468, Blast_Score=82, Evalue=4e-17, Organism=Escherichia coli, GI87082160, Length=225, Percent_Identity=27.1111111111111, Blast_Score=82, Evalue=5e-17, Organism=Escherichia coli, GI87082100, Length=192, Percent_Identity=33.8541666666667, Blast_Score=82, Evalue=5e-17, Organism=Escherichia coli, GI1787335, Length=195, Percent_Identity=30.7692307692308, Blast_Score=78, Evalue=5e-16, Organism=Escherichia coli, GI1787820, Length=180, Percent_Identity=30, Blast_Score=78, Evalue=8e-16, Organism=Escherichia coli, GI1786701, Length=186, Percent_Identity=31.1827956989247, Blast_Score=70, Evalue=1e-13, Organism=Escherichia coli, GI1789208, Length=208, Percent_Identity=30.2884615384615, Blast_Score=67, Evalue=8e-13, Organism=Escherichia coli, GI2367175, Length=211, Percent_Identity=32.7014218009479, Blast_Score=67, Evalue=1e-12, Organism=Escherichia coli, GI2367365, Length=186, Percent_Identity=32.7956989247312, Blast_Score=66, Evalue=3e-12, Organism=Escherichia coli, GI1787526, Length=192, Percent_Identity=32.2916666666667, Blast_Score=65, Evalue=4e-12, Organism=Escherichia coli, GI1789378, Length=204, Percent_Identity=31.8627450980392, Blast_Score=64, Evalue=1e-11, Organism=Caenorhabditis elegans, GI71994604, Length=194, Percent_Identity=38.659793814433, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI71994600, Length=195, Percent_Identity=35.8974358974359, Blast_Score=91, Evalue=5e-19, Organism=Caenorhabditis elegans, GI212646214, Length=195, Percent_Identity=32.8205128205128, Blast_Score=80, Evalue=1e-15, Organism=Caenorhabditis elegans, GI17557668, Length=192, Percent_Identity=31.25, Blast_Score=80, Evalue=1e-15, Organism=Caenorhabditis elegans, GI25147288, Length=184, Percent_Identity=29.8913043478261, Blast_Score=79, Evalue=2e-15, Organism=Caenorhabditis elegans, GI17559552, Length=192, Percent_Identity=29.6875, Blast_Score=77, Evalue=7e-15, Organism=Caenorhabditis elegans, GI17555706, Length=184, Percent_Identity=30.9782608695652, Blast_Score=76, Evalue=2e-14, Organism=Caenorhabditis elegans, GI115534694, Length=238, Percent_Identity=28.9915966386555, Blast_Score=74, Evalue=5e-14, Organism=Caenorhabditis elegans, GI17560676, Length=190, Percent_Identity=31.0526315789474, Blast_Score=73, Evalue=1e-13, Organism=Caenorhabditis elegans, GI25152911, Length=210, Percent_Identity=31.9047619047619, Blast_Score=69, Evalue=3e-12, Organism=Caenorhabditis elegans, GI17562910, Length=235, Percent_Identity=28.0851063829787, Blast_Score=69, Evalue=4e-12, Organism=Caenorhabditis elegans, GI17551412, Length=196, Percent_Identity=32.1428571428571, Blast_Score=66, Evalue=2e-11, Organism=Caenorhabditis elegans, GI17561402, Length=186, Percent_Identity=26.8817204301075, Blast_Score=64, Evalue=7e-11, Organism=Saccharomyces cerevisiae, GI6319635, Length=187, Percent_Identity=29.9465240641711, Blast_Score=80, Evalue=3e-16, Organism=Saccharomyces cerevisiae, GI6322067, Length=194, Percent_Identity=28.3505154639175, Blast_Score=71, Evalue=1e-13, Organism=Saccharomyces cerevisiae, GI6323882, Length=195, Percent_Identity=32.3076923076923, Blast_Score=68, Evalue=2e-12, Organism=Drosophila melanogaster, GI28571526, Length=193, Percent_Identity=39.8963730569948, Blast_Score=105, Evalue=3e-23, Organism=Drosophila melanogaster, GI24640442, Length=189, Percent_Identity=31.2169312169312, Blast_Score=99, Evalue=3e-21, Organism=Drosophila melanogaster, GI24644339, Length=193, Percent_Identity=36.7875647668394, Blast_Score=96, Evalue=2e-20, Organism=Drosophila melanogaster, GI21357041, Length=193, Percent_Identity=37.3056994818653, Blast_Score=94, Evalue=9e-20, Organism=Drosophila melanogaster, GI23397609, Length=196, Percent_Identity=36.2244897959184, Blast_Score=91, Evalue=6e-19, Organism=Drosophila melanogaster, GI24584752, Length=186, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=2e-18, Organism=Drosophila melanogaster, GI24584748, Length=186, Percent_Identity=31.7204301075269, Blast_Score=89, Evalue=3e-18, Organism=Drosophila melanogaster, GI161076933, Length=186, Percent_Identity=31.7204301075269, Blast_Score=89, Evalue=3e-18, Organism=Drosophila melanogaster, GI24639444, Length=190, Percent_Identity=33.6842105263158, Blast_Score=82, Evalue=5e-16, Organism=Drosophila melanogaster, GI24584744, Length=197, Percent_Identity=29.9492385786802, Blast_Score=81, Evalue=9e-16, Organism=Drosophila melanogaster, GI28574402, Length=197, Percent_Identity=29.9492385786802, Blast_Score=80, Evalue=1e-15, Organism=Drosophila melanogaster, GI24643142, Length=215, Percent_Identity=32.093023255814, Blast_Score=78, Evalue=5e-15, Organism=Drosophila melanogaster, GI21355319, Length=236, Percent_Identity=27.5423728813559, Blast_Score=71, Evalue=9e-13,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.69 [C]
Molecular weight: Translated: 26699; Mature: 26568
Theoretical pI: Translated: 5.74; Mature: 5.74
Prosite motif: PS00061 ADH_SHORT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 2.3 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 1.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTQAHGKALVTGASSGIGAEYAAQLAARGHDLVLVARDAARLEALAARLGGETGVAVEVL CCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEE PADLTRPADLAAVAARLAADAALRLLVNCAGLGPMGPVLPGPASGYDPMVALNVTALQHL CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHH TLAAAPAFAERGGGTIVNVSSVVALLPERFNAVYAASKAYVLALTQGLAAELAPRGVRLQ HHHHCCHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCCEEEE AVLPGITRTELFARAGADLDRIPAAMIMEARDLVAAALAGLDAGEPVTIPSLPEIDDWAA EECCCCHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHH FEAARLRLGPNLSRSAPAARYGLG HHHHHHHCCCCCCCCCCCHHCCCC >Mature Secondary Structure TQAHGKALVTGASSGIGAEYAAQLAARGHDLVLVARDAARLEALAARLGGETGVAVEVL CCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEE PADLTRPADLAAVAARLAADAALRLLVNCAGLGPMGPVLPGPASGYDPMVALNVTALQHL CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHH TLAAAPAFAERGGGTIVNVSSVVALLPERFNAVYAASKAYVLALTQGLAAELAPRGVRLQ HHHHCCHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCCEEEE AVLPGITRTELFARAGADLDRIPAAMIMEARDLVAAALAGLDAGEPVTIPSLPEIDDWAA EECCCCHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHH FEAARLRLGPNLSRSAPAARYGLG HHHHHHHCCCCCCCCCCCHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NAD+; NADH; NADP+; NADPH [C]
Metal ions: Mn2+ [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.02 {NADP+}} 20 {D-gluconate}} 0.006 {NADPH}} 0.9 {5-keto-D-gluconate}} [C]
Substrates: 5-Dehydro-D-gluconate; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]
Specific reaction: 5-Dehydro-D-gluconate + H+ + Nicotinamide adenine dinucleotide phosphate - reduced <==> D-Gluconate + Nicotinamide adenine dinucleotide phosphate [C]
General reaction: Redox reaction [C]
Inhibitor: Cd2+; Cs2+; Cu2+; Divalent metal ions; Hg2+; Ni2+; p-Chloromercuribenzoate; Sn2+; Sulfhydryl reagents [C]
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: NA