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The map label for this gene is metB [H]

Identifier: 170750797

GI number: 170750797

Start: 4676403

End: 4677563

Strand: Reverse

Name: metB [H]

Synonym: Mrad2831_4408

Alternate gene names: 170750797

Gene position: 4677563-4676403 (Counterclockwise)

Preceding gene: 170750798

Following gene: 170750794

Centisome position: 76.96

GC content: 72.52

Gene sequence:

>1161_bases
ATGTCAGAGGGATTAGCGGCGCGTACCGTCGCGGCGGCCAACGGCGTGGCCCAGGACGCGGCCTTCGGCGCGGTGATCGC
GCCGATCCACCTGTCGACGACCTTCAAGTTCCCGGCCTTCGAGCAGCCCGGCCCCTACGATTACTCGCGCCTGGGCAACC
CGACCCGGGACGCGCTGGCCGACACGATCGCCAAGCTCGAGGGCGGCGCCCGCGCCGTCCTGGTGGCGAGCGGCATGGCG
GCCCTCGACCTCGCCCTGTCGAGCCTGCGGCCCGGCGATCTCTTGGTCGCGCCGCACGATTGCTACGGCGGGACGCACCG
CCTGCTCAGCTTCCGCGCGGAGCGGGGCCATTACCGCGTCGCCTTCGTCGACCAGACCGATCCGGACGCGCTGGCGGCGG
CCCTGGCCCAGGGGCCGAAGGTCGTGCTGATCGAGACGCCCAGCAACCCGCTGATGCGCGTGGTCGATGTCCGGCGCATC
ACCGCAGACGCGAAGGCGGCCGGCGCGCTGGCTGTGGTGGACAACACCTTCCTGTCGCCCGCCCTGCAGCAGCCGATCGC
CCTCGGGGCGGATCTGGTCGTCCATTCCACCACCAAGTTCCTGAACGGCCATTCCGACGTGATCGGCGGGGTGGTGATCG
CCGCCGACAAGGCGGTGGGCGAGGACCTCGCCGCCTGGGCGAACACGATCGGCGTCACCGGCTCGCCCCTCGACGCCTAC
CTGACGCTCCGGGGCCTGCGCACCCTGTTCGTGCGGGTGGAGCAGCAGCAGCGCAACGCCGCCGCCCTGGCGGCTTTTCT
GGATGCCCATCCGGCGGTGGCCCGGGTGCATTATCCCGGCCTGCCCACGCATCCGGGGCACGCCCTGGCCAAGGCGCAGC
AGGCAGGCTTCGGGGCGATGCTGAGCTTCGAGCTGGCCGGCGGCCGCGCGGCGGTGAAGACCTTCGTCGAGGCCCTGCGG
GTGTTCACGCTGGCCGAGTCGCTCGGCGGCGTCGAGAGCCTGATCGCCCATCCGCCGACCATGACGCACGCGGCGATGGA
CCTGGAGGCGCGCGCCATCGCGGGCATCACCGACGGGCTGCTGCGCCTGTCGGTCGGCCTGGAGGCGGAGGCCGACCTGC
TGGCCGACCTGTCGCGGGCCTTCCAGGCCCTGGACGGCTGA

Upstream 100 bases:

>100_bases
AAACGGCGCTCTCGTTCTCTTGAAAGTCTGATCCGTTCTCCATAAAGAACGCACGCCCCGGTCGGACGGGGCGGGCGATC
TCTGCGTGGAGGACGAGGCG

Downstream 100 bases:

>100_bases
CCCGGCCGGTCACTCCTCCTCGACCACGCTGAACCCGGACTCGTCCTCGACCACCCGGAAACGGGCGAGCTGCACGGTCC
GGGAGCGCTCGGCCGCCTCG

Product: O-succinylhomoserine (thiol)-lyase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSEGLAARTVAAANGVAQDAAFGAVIAPIHLSTTFKFPAFEQPGPYDYSRLGNPTRDALADTIAKLEGGARAVLVASGMA
ALDLALSSLRPGDLLVAPHDCYGGTHRLLSFRAERGHYRVAFVDQTDPDALAAALAQGPKVVLIETPSNPLMRVVDVRRI
TADAKAAGALAVVDNTFLSPALQQPIALGADLVVHSTTKFLNGHSDVIGGVVIAADKAVGEDLAAWANTIGVTGSPLDAY
LTLRGLRTLFVRVEQQQRNAAALAAFLDAHPAVARVHYPGLPTHPGHALAKAQQAGFGAMLSFELAGGRAAVKTFVEALR
VFTLAESLGGVESLIAHPPTMTHAAMDLEARAIAGITDGLLRLSVGLEAEADLLADLSRAFQALDG

Sequences:

>Translated_386_residues
MSEGLAARTVAAANGVAQDAAFGAVIAPIHLSTTFKFPAFEQPGPYDYSRLGNPTRDALADTIAKLEGGARAVLVASGMA
ALDLALSSLRPGDLLVAPHDCYGGTHRLLSFRAERGHYRVAFVDQTDPDALAAALAQGPKVVLIETPSNPLMRVVDVRRI
TADAKAAGALAVVDNTFLSPALQQPIALGADLVVHSTTKFLNGHSDVIGGVVIAADKAVGEDLAAWANTIGVTGSPLDAY
LTLRGLRTLFVRVEQQQRNAAALAAFLDAHPAVARVHYPGLPTHPGHALAKAQQAGFGAMLSFELAGGRAAVKTFVEALR
VFTLAESLGGVESLIAHPPTMTHAAMDLEARAIAGITDGLLRLSVGLEAEADLLADLSRAFQALDG
>Mature_385_residues
SEGLAARTVAAANGVAQDAAFGAVIAPIHLSTTFKFPAFEQPGPYDYSRLGNPTRDALADTIAKLEGGARAVLVASGMAA
LDLALSSLRPGDLLVAPHDCYGGTHRLLSFRAERGHYRVAFVDQTDPDALAAALAQGPKVVLIETPSNPLMRVVDVRRIT
ADAKAAGALAVVDNTFLSPALQQPIALGADLVVHSTTKFLNGHSDVIGGVVIAADKAVGEDLAAWANTIGVTGSPLDAYL
TLRGLRTLFVRVEQQQRNAAALAAFLDAHPAVARVHYPGLPTHPGHALAKAQQAGFGAMLSFELAGGRAAVKTFVEALRV
FTLAESLGGVESLIAHPPTMTHAAMDLEARAIAGITDGLLRLSVGLEAEADLLADLSRAFQALDG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=344, Percent_Identity=43.6046511627907, Blast_Score=285, Evalue=6e-77,
Organism=Homo sapiens, GI299473759, Length=344, Percent_Identity=40.1162790697674, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI25453487, Length=343, Percent_Identity=38.1924198250729, Blast_Score=230, Evalue=1e-60,
Organism=Escherichia coli, GI1790375, Length=376, Percent_Identity=58.5106382978723, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1789383, Length=382, Percent_Identity=30.3664921465969, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17538055, Length=350, Percent_Identity=44.2857142857143, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17560056, Length=350, Percent_Identity=44.2857142857143, Blast_Score=288, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI71980708, Length=371, Percent_Identity=36.9272237196765, Blast_Score=227, Evalue=7e-60,
Organism=Saccharomyces cerevisiae, GI6319307, Length=368, Percent_Identity=43.2065217391304, Blast_Score=275, Evalue=9e-75,
Organism=Saccharomyces cerevisiae, GI6321254, Length=395, Percent_Identity=37.4683544303797, Blast_Score=222, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6323333, Length=421, Percent_Identity=31.8289786223278, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321904, Length=396, Percent_Identity=28.7878787878788, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6321170, Length=322, Percent_Identity=29.5031055900621, Blast_Score=126, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6322970, Length=258, Percent_Identity=27.1317829457364, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922554, Length=381, Percent_Identity=41.4698162729659, Blast_Score=290, Evalue=9e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 40046; Mature: 39915

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEGLAARTVAAANGVAQDAAFGAVIAPIHLSTTFKFPAFEQPGPYDYSRLGNPTRDALA
CCCCHHHHHHHHHCCCHHHHHCCEEEEEEEEEEEECCCCCCCCCCCCHHHCCCCHHHHHH
DTIAKLEGGARAVLVASGMAALDLALSSLRPGDLLVAPHDCYGGTHRLLSFRAERGHYRV
HHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCEEE
AFVDQTDPDALAAALAQGPKVVLIETPSNPLMRVVDVRRITADAKAAGALAVVDNTFLSP
EEECCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCHHHCCEEEEECCHHHCH
ALQQPIALGADLVVHSTTKFLNGHSDVIGGVVIAADKAVGEDLAAWANTIGVTGSPLDAY
HHHCHHHHCCCCEEECHHHHHCCCCHHHCCEEEEECHHHHHHHHHHHHHCCCCCCCHHHH
LTLRGLRTLFVRVEQQQRNAAALAAFLDAHPAVARVHYPGLPTHPGHALAKAQQAGFGAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEE
LSFELAGGRAAVKTFVEALRVFTLAESLGGVESLIAHPPTMTHAAMDLEARAIAGITDGL
EEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHCCHHHHHHHHHHHH
LRLSVGLEAEADLLADLSRAFQALDG
HHEECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEGLAARTVAAANGVAQDAAFGAVIAPIHLSTTFKFPAFEQPGPYDYSRLGNPTRDALA
CCCHHHHHHHHHCCCHHHHHCCEEEEEEEEEEEECCCCCCCCCCCCHHHCCCCHHHHHH
DTIAKLEGGARAVLVASGMAALDLALSSLRPGDLLVAPHDCYGGTHRLLSFRAERGHYRV
HHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCEEE
AFVDQTDPDALAAALAQGPKVVLIETPSNPLMRVVDVRRITADAKAAGALAVVDNTFLSP
EEECCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCHHHCCEEEEECCHHHCH
ALQQPIALGADLVVHSTTKFLNGHSDVIGGVVIAADKAVGEDLAAWANTIGVTGSPLDAY
HHHCHHHHCCCCEEECHHHHHCCCCHHHCCEEEEECHHHHHHHHHHHHHCCCCCCCHHHH
LTLRGLRTLFVRVEQQQRNAAALAAFLDAHPAVARVHYPGLPTHPGHALAKAQQAGFGAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEE
LSFELAGGRAAVKTFVEALRVFTLAESLGGVESLIAHPPTMTHAAMDLEARAIAGITDGL
EEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHCCHHHHHHHHHHHH
LRLSVGLEAEADLLADLSRAFQALDG
HHEECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]