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The map label for this gene is amiC [H]

Identifier: 170750747

GI number: 170750747

Start: 4620006

End: 4621265

Strand: Direct

Name: amiC [H]

Synonym: Mrad2831_4358

Alternate gene names: 170750747

Gene position: 4620006-4621265 (Clockwise)

Preceding gene: 170750746

Following gene: 170750748

Centisome position: 76.01

GC content: 74.29

Gene sequence:

>1260_bases
ATGCCGAACTGCGCGCGCCTGATCCGTTCCGCATGGCTCCGCCTCGCCGTCGCGCTCCTGCCGGCGCTGGCGGGGGCGCC
GGTCGCCGCGCAGACCGGCGAGAAGCCGGCCGTCGCCATCGGGGCCGACCTCGCGAGCCAGGCCGGCACCACGCGCCTCA
CCCTGACGCTGTCCCGGGCCGTGGAGGCCCGCGGCTTCGTGATGGAGCGGCCCGACCGGGCGGTGATCGACCTGCCCGAG
GTCAATTTCCAGCTCGATTCTGGGACCGGCAGCCGCCGCGACGGCCTCGTGCAGAGCTTCCGCTACGGCCTGTTCGCGCC
CGGGCGGTCCCGCATCGTGATCGACCTCACCGCGCCCGCCACCGTCGGGAAGATCGAGACCGCGACGCGGCCGCGCGACG
GCGCGGTTCTGCTCACGATCCCGCTGCAGCGCGCCGACCGCGACGCGTTCCGCAAGGCCGCCGCTACCGCCGATCCCGCC
CCCGCCCAGGCCCGCGCCGCGCCGCCGGAGCCCGCCGACCAGCGGCCGCTGATCATGGTCGATGCCGGCCACGGCGGCAC
CGATCCCGGCGCCATCGCGGTGGGCGGCGTGTTCGAGAAGGACATCGTGTTCGGCTTCGCCCAGGCCCTCGCGGCGAAGC
TGGAGGCGGGCGGCCGCTACCGGGTCCGGATGACCCGCGACCGCGACGTGTTCGTGCCCCTGGCCGAGCGGGTGCGCCTC
GCCCGCGACGCCAGGGCCGACCTGTTCGTGTCGATCCACGCGGACTCGATCTCGGCGGCGCCCCAGGTGAGCGGCGCCAC
GATCTACACCAATTCCGAGAAGGCCACGGACGCCGAATCCGCCAAGCTCGCCGAGCGCGAGAACCGGGCCGATTCCGCCG
CGGGCGTCGACGCCGCCGAGGCGCCGCCGGACATCGCCGACATCCTGCAGGAGCTGACCCTGCGCGAGACGCGGGGCTTC
TCGTCGGGCTTCGCCCGCACGCTGATGGGCCAGCTCGCCCCCGTGATGAAGATGAGCGCCAAGCCCCACCGGGAGGCGCG
CTTCGTGGTGCTGCGCTCCCCCGACGTGCCGAGCGTGCTCGTGGAACTCGGCTACCTCTCCAGCAAGCGCGACCTGGAGA
TGCTGCAATCGCCGGAGTGGCGGGCCGACGTCACCGGCTCCATGGCCAAGGCGATCGACCAGTTCTTCACCAACCGCGCC
ACGCTCGGCCGGCCGGCCCCGACGCGCCGCGCGGCCGCCGCCGCCCCAGTTTCACCATAG

Upstream 100 bases:

>100_bases
CGGCCACAAGGCCGGCCGGATCGAACCGGCGACGGCAACCCCCCGTTAACCAAGTCGGTCCCATGCCTTAACGGAGGGCA
CGGGAGACGACACCGGACCG

Downstream 100 bases:

>100_bases
ATAGAGTGTCGATACGGGACGTCCGACGCCCCGTCGCACGGCCGCCGAAACCGGCATTCCGAGGGGGTCTCACGGGACCG
TCCGGGGGCCGGCCGCGCAG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MPNCARLIRSAWLRLAVALLPALAGAPVAAQTGEKPAVAIGADLASQAGTTRLTLTLSRAVEARGFVMERPDRAVIDLPE
VNFQLDSGTGSRRDGLVQSFRYGLFAPGRSRIVIDLTAPATVGKIETATRPRDGAVLLTIPLQRADRDAFRKAAATADPA
PAQARAAPPEPADQRPLIMVDAGHGGTDPGAIAVGGVFEKDIVFGFAQALAAKLEAGGRYRVRMTRDRDVFVPLAERVRL
ARDARADLFVSIHADSISAAPQVSGATIYTNSEKATDAESAKLAERENRADSAAGVDAAEAPPDIADILQELTLRETRGF
SSGFARTLMGQLAPVMKMSAKPHREARFVVLRSPDVPSVLVELGYLSSKRDLEMLQSPEWRADVTGSMAKAIDQFFTNRA
TLGRPAPTRRAAAAAPVSP

Sequences:

>Translated_419_residues
MPNCARLIRSAWLRLAVALLPALAGAPVAAQTGEKPAVAIGADLASQAGTTRLTLTLSRAVEARGFVMERPDRAVIDLPE
VNFQLDSGTGSRRDGLVQSFRYGLFAPGRSRIVIDLTAPATVGKIETATRPRDGAVLLTIPLQRADRDAFRKAAATADPA
PAQARAAPPEPADQRPLIMVDAGHGGTDPGAIAVGGVFEKDIVFGFAQALAAKLEAGGRYRVRMTRDRDVFVPLAERVRL
ARDARADLFVSIHADSISAAPQVSGATIYTNSEKATDAESAKLAERENRADSAAGVDAAEAPPDIADILQELTLRETRGF
SSGFARTLMGQLAPVMKMSAKPHREARFVVLRSPDVPSVLVELGYLSSKRDLEMLQSPEWRADVTGSMAKAIDQFFTNRA
TLGRPAPTRRAAAAAPVSP
>Mature_418_residues
PNCARLIRSAWLRLAVALLPALAGAPVAAQTGEKPAVAIGADLASQAGTTRLTLTLSRAVEARGFVMERPDRAVIDLPEV
NFQLDSGTGSRRDGLVQSFRYGLFAPGRSRIVIDLTAPATVGKIETATRPRDGAVLLTIPLQRADRDAFRKAAATADPAP
AQARAAPPEPADQRPLIMVDAGHGGTDPGAIAVGGVFEKDIVFGFAQALAAKLEAGGRYRVRMTRDRDVFVPLAERVRLA
RDARADLFVSIHADSISAAPQVSGATIYTNSEKATDAESAKLAERENRADSAAGVDAAEAPPDIADILQELTLRETRGFS
SGFARTLMGQLAPVMKMSAKPHREARFVVLRSPDVPSVLVELGYLSSKRDLEMLQSPEWRADVTGSMAKAIDQFFTNRAT
LGRPAPTRRAAAAAPVSP

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=373, Percent_Identity=30.2949061662198, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1788776, Length=223, Percent_Identity=34.5291479820628, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790611, Length=227, Percent_Identity=30.3964757709251, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 44500; Mature: 44369

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNCARLIRSAWLRLAVALLPALAGAPVAAQTGEKPAVAIGADLASQAGTTRLTLTLSRA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHCCCCEEEEEEEHHH
VEARGFVMERPDRAVIDLPEVNFQLDSGTGSRRDGLVQSFRYGLFAPGRSRIVIDLTAPA
HHHCCCEEECCCCCEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCC
TVGKIETATRPRDGAVLLTIPLQRADRDAFRKAAATADPAPAQARAAPPEPADQRPLIMV
CCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEE
DAGHGGTDPGAIAVGGVFEKDIVFGFAQALAAKLEAGGRYRVRMTRDRDVFVPLAERVRL
ECCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEECHHHHHHH
ARDARADLFVSIHADSISAAPQVSGATIYTNSEKATDAESAKLAERENRADSAAGVDAAE
HHCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCHHCCCCCCC
APPDIADILQELTLRETRGFSSGFARTLMGQLAPVMKMSAKPHREARFVVLRSPDVPSVL
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHH
VELGYLSSKRDLEMLQSPEWRADVTGSMAKAIDQFFTNRATLGRPAPTRRAAAAAPVSP
HHHHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
PNCARLIRSAWLRLAVALLPALAGAPVAAQTGEKPAVAIGADLASQAGTTRLTLTLSRA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHCCCCEEEEEEEHHH
VEARGFVMERPDRAVIDLPEVNFQLDSGTGSRRDGLVQSFRYGLFAPGRSRIVIDLTAPA
HHHCCCEEECCCCCEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCC
TVGKIETATRPRDGAVLLTIPLQRADRDAFRKAAATADPAPAQARAAPPEPADQRPLIMV
CCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEE
DAGHGGTDPGAIAVGGVFEKDIVFGFAQALAAKLEAGGRYRVRMTRDRDVFVPLAERVRL
ECCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEECHHHHHHH
ARDARADLFVSIHADSISAAPQVSGATIYTNSEKATDAESAKLAERENRADSAAGVDAAE
HHCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCHHCCCCCCC
APPDIADILQELTLRETRGFSSGFARTLMGQLAPVMKMSAKPHREARFVVLRSPDVPSVL
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHH
VELGYLSSKRDLEMLQSPEWRADVTGSMAKAIDQFFTNRATLGRPAPTRRAAAAAPVSP
HHHHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]