| Definition | Methylobacterium radiotolerans JCM 2831 chromosome, complete genome. |
|---|---|
| Accession | NC_010505 |
| Length | 6,077,833 |
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The map label for this gene is mrcA [H]
Identifier: 170750748
GI number: 170750748
Start: 4621436
End: 4623868
Strand: Direct
Name: mrcA [H]
Synonym: Mrad2831_4359
Alternate gene names: 170750748
Gene position: 4621436-4623868 (Clockwise)
Preceding gene: 170750747
Following gene: 170750754
Centisome position: 76.04
GC content: 69.79
Gene sequence:
>2433_bases ATGCGTCTGATCCTCCGTTTCTTTGGGATCCTGTTCAGCGCCGCCGCGGTGCTGTTCATGGTCGGCGCGGCGGTGGGCGG CTACTTCTACTGGAAGTACAGCCAGGACCTGCCCGACCACGCCGCCCTCGCGAATTACGAGCCGCCGGTGATGACCCGCG TCCACGCGGCGGACGGCTCCCTGCTGGCCGAGTACGCCCGCGAGCGCCGGCTGTACCTACCGATCCAGGCGATGCCGAAG ATCGTCGTGGCGGCCTTCCTGTCGGCCGAGGACAAGAACTTCTACAAGCACGGCGGCATCGACCCCGAGGGGATCGTGCG CGCCTTCCTGACCAACGCCAAGTCGGGCAAGCGGCAGGGCGCCTCGACCATCACCCAGCAGGTCGCCAAGAACTTCCTGC TCTCCTCCGAGCAGACCTACGACCGCAAGATCAAGGAGGCGCTGCTGGCGCTCCGGATCGAGGCGGCCTACTCGAAGGAC AAGATCCTCGAGCTCTACCTCAACGAGATCTTCCTCGGCACGATCGTGCCCGGCCGCAACCTGCACGGCGTCGCGGCCGC CGCGCTCGACTATTTCGGCAAGTCGGTCCACGAGCTGACCATCAACGAGGCGGCCTACCTCGCCGCCCTGCCGAAGGGGC CGAACAACTACCACCCCTACCGCAAGACCCAGGCGGCCCTCGACCGCCGCAACGAGATCATCCGCCTGATGGCGGAGAAC GGCTACATCACCCGGGAGGAGGCGGATTCCGCCAAGAAGATGCCGCTCGGCGTCAACCCGCGCGTCGCCTTCCCGAACGC CGCCAACGCCAACTACTTCACCGAGGAGGTCCGCCGCGAGATCTCCGAGCGCTACGGCGAGAAGAAGCTCTACGAGGGCG GCCTCTCGGTGCGCACGACCCTCGACCCGAAGATGCAGGCCTGGGCCCGCAAGGCGCTGGTCGACGGCCTCGTGCGCTAC GACCAGGCCCATGGCTGGCGCGGTCCGGTCACCAAGGTCGACCTGACCGGGCGCGACTGGGGCATGGCGGCGGCCGAGGT TCCGGCGCTCGGCGACGTCGCGCCCTGGCGGCTCGCCGTGGTGCTGGGCAACGGCGGCGGCGGCGTGCAGATCGGCCTGC AGCCCCGGCGCGAGGCCTCGGGCCAGGTCGGCAAGGACCGCGAGACCGGCGTGATCACCCCCGAGGGCATGCGCTGGGCC GGCCGCGCCAATCTCCAGCCCGGCGACATGGTCTACGTCGAGGCGATGGACGGCGGCCGCGGCCAGTTCCGCCTGCGTCA GAAGCCCGAGGTCTCCGGCGCCATCGTGGCCATGGACCCGAATACGGGCCGCGTGCACGCGATGGTCGGCGGCTTCTCGT ACGACGAGTCCGAGTTCAACCGCGCCACCCAGGCGATGCGCCAGCCCGGCTCCTCGTTCAAGCCGATCGTCTACTCGGCG GCGCTGGACAACGGCTACACGCCCGCCTCGATCATCCAGGACACCCCGATCACCATCGAGGCCGGTCCCGGCCAGGAGGC CTGGACGCCCTCGAACTACGACGGCAAGTCGGGCGGCCCGCACACCCTGCGCTACGGCATCGAGCACTCGAAGAACCTGA TGACGGTCCGGCTCGCCAAGGATGTCGGCATGCCGCTCATCGCCGAGTACGCACGCCGCTTCGGCGTCTACGACGACATG CTGCCGGTGCTGCCCATGTCGCTCGGCGCGGGTGAGACCACGGTGATGCGCATGGTCACCGCCTACTCGATGCTCGCCAA CGGCGGCCGGCGCATCCGGCCGACCCTGATCGACCGGATCCAGGACCGCATCGGCGAGACGATCTACCGGCACGACAACC GCAAGTGCATCGGCTGCGACGCCGAGAAATGGTCGGGCCAGGACGAGCCGAAGCTGGTGGACGATTCCGAGCAGGTGCTC GACCCGCTGACCGCCTACCAGATGGTCTCGATCATGGAGGGGGTGGTCCAGCGCGGCACCGCCACGATCCTGAAGCAGAT CGGCAAGCCGCTGGCCGGCAAGACCGGCACGACGAACGACGCCAAGGACGCGTGGTTCGTGGGCTTCTCGCCGGATCTGG CGGTCGGCATCTATATCGGCTTCGACAAGCCGCGGTCGCTGGGCGACCGCGCCACCGGCGGCGGCTTGGCCGCGCCGATC GCCCTGGAGTTCCTGAAGACGGCCCTCAAGGACAAGCCGCCGACGCCGTTCCGCGTGCCGCCGGGGATCAAGCTGATCCG CATCAACCTCGCGAGCGGCACCCGCGCCGGTTCGGGGGAGGGCGCCGGGACGATCCTGGAGGCGTTCAAGCCCGGCACCG CGCCGCCGGATTCCTACTCGGCGCCGTCCTCCTCGGGCGGCGGCCAGGCGGCCGCCCCCGCGGCGGTCGCGCCCGATCCC GACCGGGCCGCGCAGGCCGGCGGGCTGTACTGA
Upstream 100 bases:
>100_bases CACGGGACCGTCCGGGGGCCGGCCGCGCAGCGCGCGGGGTGCACGCGCCGGATGAGCCGATTCCGGCATTCGGCCGGGCC AGCGACGAGCGGAACACCGG
Downstream 100 bases:
>100_bases GGCGGCTCGCCCCGCCCAGCCCGCACTCAGCCGAGGTCGCACTCAGCCCAGGTCGGGTCCGCCCGGCCTGGGCTCTCGCG TTGTGGATCCGGAGCGGACG
Product: 1A family penicillin-binding protein
Products: NA
Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 810; Mature: 810
Protein sequence:
>810_residues MRLILRFFGILFSAAAVLFMVGAAVGGYFYWKYSQDLPDHAALANYEPPVMTRVHAADGSLLAEYARERRLYLPIQAMPK IVVAAFLSAEDKNFYKHGGIDPEGIVRAFLTNAKSGKRQGASTITQQVAKNFLLSSEQTYDRKIKEALLALRIEAAYSKD KILELYLNEIFLGTIVPGRNLHGVAAAALDYFGKSVHELTINEAAYLAALPKGPNNYHPYRKTQAALDRRNEIIRLMAEN GYITREEADSAKKMPLGVNPRVAFPNAANANYFTEEVRREISERYGEKKLYEGGLSVRTTLDPKMQAWARKALVDGLVRY DQAHGWRGPVTKVDLTGRDWGMAAAEVPALGDVAPWRLAVVLGNGGGGVQIGLQPRREASGQVGKDRETGVITPEGMRWA GRANLQPGDMVYVEAMDGGRGQFRLRQKPEVSGAIVAMDPNTGRVHAMVGGFSYDESEFNRATQAMRQPGSSFKPIVYSA ALDNGYTPASIIQDTPITIEAGPGQEAWTPSNYDGKSGGPHTLRYGIEHSKNLMTVRLAKDVGMPLIAEYARRFGVYDDM LPVLPMSLGAGETTVMRMVTAYSMLANGGRRIRPTLIDRIQDRIGETIYRHDNRKCIGCDAEKWSGQDEPKLVDDSEQVL DPLTAYQMVSIMEGVVQRGTATILKQIGKPLAGKTGTTNDAKDAWFVGFSPDLAVGIYIGFDKPRSLGDRATGGGLAAPI ALEFLKTALKDKPPTPFRVPPGIKLIRINLASGTRAGSGEGAGTILEAFKPGTAPPDSYSAPSSSGGGQAAAPAAVAPDP DRAAQAGGLY
Sequences:
>Translated_810_residues MRLILRFFGILFSAAAVLFMVGAAVGGYFYWKYSQDLPDHAALANYEPPVMTRVHAADGSLLAEYARERRLYLPIQAMPK IVVAAFLSAEDKNFYKHGGIDPEGIVRAFLTNAKSGKRQGASTITQQVAKNFLLSSEQTYDRKIKEALLALRIEAAYSKD KILELYLNEIFLGTIVPGRNLHGVAAAALDYFGKSVHELTINEAAYLAALPKGPNNYHPYRKTQAALDRRNEIIRLMAEN GYITREEADSAKKMPLGVNPRVAFPNAANANYFTEEVRREISERYGEKKLYEGGLSVRTTLDPKMQAWARKALVDGLVRY DQAHGWRGPVTKVDLTGRDWGMAAAEVPALGDVAPWRLAVVLGNGGGGVQIGLQPRREASGQVGKDRETGVITPEGMRWA GRANLQPGDMVYVEAMDGGRGQFRLRQKPEVSGAIVAMDPNTGRVHAMVGGFSYDESEFNRATQAMRQPGSSFKPIVYSA ALDNGYTPASIIQDTPITIEAGPGQEAWTPSNYDGKSGGPHTLRYGIEHSKNLMTVRLAKDVGMPLIAEYARRFGVYDDM LPVLPMSLGAGETTVMRMVTAYSMLANGGRRIRPTLIDRIQDRIGETIYRHDNRKCIGCDAEKWSGQDEPKLVDDSEQVL DPLTAYQMVSIMEGVVQRGTATILKQIGKPLAGKTGTTNDAKDAWFVGFSPDLAVGIYIGFDKPRSLGDRATGGGLAAPI ALEFLKTALKDKPPTPFRVPPGIKLIRINLASGTRAGSGEGAGTILEAFKPGTAPPDSYSAPSSSGGGQAAAPAAVAPDP DRAAQAGGLY >Mature_810_residues MRLILRFFGILFSAAAVLFMVGAAVGGYFYWKYSQDLPDHAALANYEPPVMTRVHAADGSLLAEYARERRLYLPIQAMPK IVVAAFLSAEDKNFYKHGGIDPEGIVRAFLTNAKSGKRQGASTITQQVAKNFLLSSEQTYDRKIKEALLALRIEAAYSKD KILELYLNEIFLGTIVPGRNLHGVAAAALDYFGKSVHELTINEAAYLAALPKGPNNYHPYRKTQAALDRRNEIIRLMAEN GYITREEADSAKKMPLGVNPRVAFPNAANANYFTEEVRREISERYGEKKLYEGGLSVRTTLDPKMQAWARKALVDGLVRY DQAHGWRGPVTKVDLTGRDWGMAAAEVPALGDVAPWRLAVVLGNGGGGVQIGLQPRREASGQVGKDRETGVITPEGMRWA GRANLQPGDMVYVEAMDGGRGQFRLRQKPEVSGAIVAMDPNTGRVHAMVGGFSYDESEFNRATQAMRQPGSSFKPIVYSA ALDNGYTPASIIQDTPITIEAGPGQEAWTPSNYDGKSGGPHTLRYGIEHSKNLMTVRLAKDVGMPLIAEYARRFGVYDDM LPVLPMSLGAGETTVMRMVTAYSMLANGGRRIRPTLIDRIQDRIGETIYRHDNRKCIGCDAEKWSGQDEPKLVDDSEQVL DPLTAYQMVSIMEGVVQRGTATILKQIGKPLAGKTGTTNDAKDAWFVGFSPDLAVGIYIGFDKPRSLGDRATGGGLAAPI ALEFLKTALKDKPPTPFRVPPGIKLIRINLASGTRAGSGEGAGTILEAFKPGTAPPDSYSAPSSSGGGQAAAPAAVAPDP DRAAQAGGLY
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG5009
COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI87082258, Length=856, Percent_Identity=34.1121495327103, Blast_Score=422, Evalue=1e-119, Organism=Escherichia coli, GI1786343, Length=275, Percent_Identity=40.3636363636364, Blast_Score=178, Evalue=1e-45, Organism=Escherichia coli, GI1788867, Length=212, Percent_Identity=33.9622641509434, Blast_Score=112, Evalue=1e-25, Organism=Escherichia coli, GI1789601, Length=158, Percent_Identity=38.6075949367089, Blast_Score=87, Evalue=3e-18,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011816 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.2.-; 3.4.-.-
Molecular weight: Translated: 87731; Mature: 87731
Theoretical pI: Translated: 9.35; Mature: 9.35
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 3.1 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 2.8 %Met (Mature Protein) 3.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRLILRFFGILFSAAAVLFMVGAAVGGYFYWKYSQDLPDHAALANYEPPVMTRVHAADGS CHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHCCCCCCCEEEEECCCCH LLAEYARERRLYLPIQAMPKIVVAAFLSAEDKNFYKHGGIDPEGIVRAFLTNAKSGKRQG HHHHHHHCCEEEECHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHCCCCCCHHH ASTITQQVAKNFLLSSEQTYDRKIKEALLALRIEAAYSKDKILELYLNEIFLGTIVPGRN HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCC LHGVAAAALDYFGKSVHELTINEAAYLAALPKGPNNYHPYRKTQAALDRRNEIIRLMAEN CCHHHHHHHHHHCCHHEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEEEECC GYITREEADSAKKMPLGVNPRVAFPNAANANYFTEEVRREISERYGEKKLYEGGLSVRTT CEEEHHHCCCHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCEEEEE LDPKMQAWARKALVDGLVRYDQAHGWRGPVTKVDLTGRDWGMAAAEVPALGDVAPWRLAV CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHCCCCCCCCCCEEEEE VLGNGGGGVQIGLQPRREASGQVGKDRETGVITPEGMRWAGRANLQPGDMVYVEAMDGGR EEECCCCEEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCC GQFRLRQKPEVSGAIVAMDPNTGRVHAMVGGFSYDESEFNRATQAMRQPGSSFKPIVYSA CCEEECCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEE ALDNGYTPASIIQDTPITIEAGPGQEAWTPSNYDGKSGGPHTLRYGIEHSKNLMTVRLAK ECCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEHH DVGMPLIAEYARRFGVYDDMLPVLPMSLGAGETTVMRMVTAYSMLANGGRRIRPTLIDRI HCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEECHHHHHHH QDRIGETIYRHDNRKCIGCDAEKWSGQDEPKLVDDSEQVLDPLTAYQMVSIMEGVVQRGT HHHHHHHHHHCCCCEEEECCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCH ATILKQIGKPLAGKTGTTNDAKDAWFVGFSPDLAVGIYIGFDKPRSLGDRATGGGLAAPI HHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHH ALEFLKTALKDKPPTPFRVPPGIKLIRINLASGTRAGSGEGAGTILEAFKPGTAPPDSYS HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCC APSSSGGGQAAAPAAVAPDPDRAAQAGGLY CCCCCCCCCCCCCCCCCCCCHHHHHCCCCC >Mature Secondary Structure MRLILRFFGILFSAAAVLFMVGAAVGGYFYWKYSQDLPDHAALANYEPPVMTRVHAADGS CHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHCCCCCCCEEEEECCCCH LLAEYARERRLYLPIQAMPKIVVAAFLSAEDKNFYKHGGIDPEGIVRAFLTNAKSGKRQG HHHHHHHCCEEEECHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHCCCCCCHHH ASTITQQVAKNFLLSSEQTYDRKIKEALLALRIEAAYSKDKILELYLNEIFLGTIVPGRN HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCC LHGVAAAALDYFGKSVHELTINEAAYLAALPKGPNNYHPYRKTQAALDRRNEIIRLMAEN CCHHHHHHHHHHCCHHEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEEEECC GYITREEADSAKKMPLGVNPRVAFPNAANANYFTEEVRREISERYGEKKLYEGGLSVRTT CEEEHHHCCCHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCEEEEE LDPKMQAWARKALVDGLVRYDQAHGWRGPVTKVDLTGRDWGMAAAEVPALGDVAPWRLAV CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHCCCCCCCCCCEEEEE VLGNGGGGVQIGLQPRREASGQVGKDRETGVITPEGMRWAGRANLQPGDMVYVEAMDGGR EEECCCCEEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCC GQFRLRQKPEVSGAIVAMDPNTGRVHAMVGGFSYDESEFNRATQAMRQPGSSFKPIVYSA CCEEECCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEE ALDNGYTPASIIQDTPITIEAGPGQEAWTPSNYDGKSGGPHTLRYGIEHSKNLMTVRLAK ECCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEHH DVGMPLIAEYARRFGVYDDMLPVLPMSLGAGETTVMRMVTAYSMLANGGRRIRPTLIDRI HCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEECHHHHHHH QDRIGETIYRHDNRKCIGCDAEKWSGQDEPKLVDDSEQVLDPLTAYQMVSIMEGVVQRGT HHHHHHHHHHCCCCEEEECCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCH ATILKQIGKPLAGKTGTTNDAKDAWFVGFSPDLAVGIYIGFDKPRSLGDRATGGGLAAPI HHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHH ALEFLKTALKDKPPTPFRVPPGIKLIRINLASGTRAGSGEGAGTILEAFKPGTAPPDSYS HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCC APSSSGGGQAAAPAAVAPDPDRAAQAGGLY CCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 11557893 [H]