Click here to switch to the map view.

The map label for this gene is ispH [H]

Identifier: 170750702

GI number: 170750702

Start: 4561674

End: 4562675

Strand: Direct

Name: ispH [H]

Synonym: Mrad2831_4312

Alternate gene names: 170750702

Gene position: 4561674-4562675 (Clockwise)

Preceding gene: 170750701

Following gene: 170750703

Centisome position: 75.05

GC content: 69.76

Gene sequence:

>1002_bases
ATGACCGCCGATATCGCGACCCCCGAGCCCGCCCCGGGCACCGCCGGCAAGCCGCCGCTGGAGATCCTGCTCTGCGCCCC
GCGCGGCTTCTGCGCCGGCGTGGTGCGGGCCATCGACGTCGTGGAGCGGGCGCTCGCCATCTACGGGCCGCCCGTCTACG
TCCGCCACGAGATCGTGCACAACAAGTACGTCGTGGAGAGCCTGAAGCGGAAGGGCGCCGTGTTCGTCCGCGAGCTCGAC
GAGGTGCCGGACGGCGGCGCCCCGGTGATCTTCTCCGCCCACGGCGTGGCCAAGACCGTGCCGGCCAACGCTGATTCGCG
CGGGCTGACCACGATCGACGCCACCTGCCCGCTGGTGACCAAGGTCCATCGCGAGGCTGAGATCCACCACAAGCGCGGCC
GCCACGTGCTGCTGGTCGGCCATTCCGGCCATCCCGAGGTCGTGGGCACGATGGGCCAGCTGCCCAAGGGCTCGATCACC
CTGGTGGAGGATCTCGAGCAGATCGCCGCCCTGACCCCGGACAATCCCGACAACCTCGCCTGGGTCACCCAGACGACCCT
GTCGGTCGACGACACCCGCCACATCGTCGACGCCCTGAAGAAGAAGTTCCCGAACATCACCGGGCCCCACAAGGACGACA
TCTGCTACGCCACCACCAACCGCCAGGAGGCGGTGAAGCAGGTGGCGCCGCTGGTCGACGCCCTGATCGTGGTCGGCTCG
TCGAACTCGTCGAACTCGCAGCGCCTGCGCGAGGTCGCCGAGCGGGTGGGCTGCCCGATCACCCGCCTCGTGCTCCGCGC
CGAGGAGATCGACTGGCCGGCCTTCGAGGGAATCCGCAAGCTCGGCCTCACGGCAGGCGCCTCGGCCCCCGAGGTCCTGG
TCGAGGAGATCATCGACGCCTTCGCGGCCCGCTACGACGTGATGGTCGACACGGTCTCGACGGTGATCGAGGACATGGTC
TTCCCGTTGCCGCGGGAGCTGCGCAGCGAGGCCGCCGAGTAA

Upstream 100 bases:

>100_bases
GCTACAGACGGGCCACGGAGCGGCTCCGCTTCCCTCTTCCCGCCCGGTTCCTGGGCCAGTCCTGTTGACAGGACAGCCCC
GCCCTCGCCACATCCCCCGC

Downstream 100 bases:

>100_bases
GGCGGTCCGGTCGACGGCGCGGGACCGACGCCTCGGTCTCGGGGCGCGCGGCAGGCTTTGTGCGGGGGCGCTTCGGCGCC
CCTTGCCGTATCGAACTTCC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTADIATPEPAPGTAGKPPLEILLCAPRGFCAGVVRAIDVVERALAIYGPPVYVRHEIVHNKYVVESLKRKGAVFVRELD
EVPDGGAPVIFSAHGVAKTVPANADSRGLTTIDATCPLVTKVHREAEIHHKRGRHVLLVGHSGHPEVVGTMGQLPKGSIT
LVEDLEQIAALTPDNPDNLAWVTQTTLSVDDTRHIVDALKKKFPNITGPHKDDICYATTNRQEAVKQVAPLVDALIVVGS
SNSSNSQRLREVAERVGCPITRLVLRAEEIDWPAFEGIRKLGLTAGASAPEVLVEEIIDAFAARYDVMVDTVSTVIEDMV
FPLPRELRSEAAE

Sequences:

>Translated_333_residues
MTADIATPEPAPGTAGKPPLEILLCAPRGFCAGVVRAIDVVERALAIYGPPVYVRHEIVHNKYVVESLKRKGAVFVRELD
EVPDGGAPVIFSAHGVAKTVPANADSRGLTTIDATCPLVTKVHREAEIHHKRGRHVLLVGHSGHPEVVGTMGQLPKGSIT
LVEDLEQIAALTPDNPDNLAWVTQTTLSVDDTRHIVDALKKKFPNITGPHKDDICYATTNRQEAVKQVAPLVDALIVVGS
SNSSNSQRLREVAERVGCPITRLVLRAEEIDWPAFEGIRKLGLTAGASAPEVLVEEIIDAFAARYDVMVDTVSTVIEDMV
FPLPRELRSEAAE
>Mature_332_residues
TADIATPEPAPGTAGKPPLEILLCAPRGFCAGVVRAIDVVERALAIYGPPVYVRHEIVHNKYVVESLKRKGAVFVRELDE
VPDGGAPVIFSAHGVAKTVPANADSRGLTTIDATCPLVTKVHREAEIHHKRGRHVLLVGHSGHPEVVGTMGQLPKGSITL
VEDLEQIAALTPDNPDNLAWVTQTTLSVDDTRHIVDALKKKFPNITGPHKDDICYATTNRQEAVKQVAPLVDALIVVGSS
NSSNSQRLREVAERVGCPITRLVLRAEEIDWPAFEGIRKLGLTAGASAPEVLVEEIIDAFAARYDVMVDTVSTVIEDMVF
PLPRELRSEAAE

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=316, Percent_Identity=52.2151898734177, Blast_Score=324, Evalue=6e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 35944; Mature: 35812

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADIATPEPAPGTAGKPPLEILLCAPRGFCAGVVRAIDVVERALAIYGPPVYVRHEIVH
CCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NKYVVESLKRKGAVFVRELDEVPDGGAPVIFSAHGVAKTVPANADSRGLTTIDATCPLVT
HHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEECCCCHHCCCCCCCCCCCEEECCCCHHHH
KVHREAEIHHKRGRHVLLVGHSGHPEVVGTMGQLPKGSITLVEDLEQIAALTPDNPDNLA
HHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEE
WVTQTTLSVDDTRHIVDALKKKFPNITGPHKDDICYATTNRQEAVKQVAPLVDALIVVGS
EEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHEECC
SNSSNSQRLREVAERVGCPITRLVLRAEEIDWPAFEGIRKLGLTAGASAPEVLVEEIIDA
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
FAARYDVMVDTVSTVIEDMVFPLPRELRSEAAE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure 
TADIATPEPAPGTAGKPPLEILLCAPRGFCAGVVRAIDVVERALAIYGPPVYVRHEIVH
CCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NKYVVESLKRKGAVFVRELDEVPDGGAPVIFSAHGVAKTVPANADSRGLTTIDATCPLVT
HHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEECCCCHHCCCCCCCCCCCEEECCCCHHHH
KVHREAEIHHKRGRHVLLVGHSGHPEVVGTMGQLPKGSITLVEDLEQIAALTPDNPDNLA
HHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEE
WVTQTTLSVDDTRHIVDALKKKFPNITGPHKDDICYATTNRQEAVKQVAPLVDALIVVGS
EEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHEECC
SNSSNSQRLREVAERVGCPITRLVLRAEEIDWPAFEGIRKLGLTAGASAPEVLVEEIIDA
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
FAARYDVMVDTVSTVIEDMVFPLPRELRSEAAE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA