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The map label for this gene is phaZ1 [H]

Identifier: 170750671

GI number: 170750671

Start: 4532743

End: 4533954

Strand: Reverse

Name: phaZ1 [H]

Synonym: Mrad2831_4281

Alternate gene names: 170750671

Gene position: 4533954-4532743 (Counterclockwise)

Preceding gene: 170750672

Following gene: 170750670

Centisome position: 74.6

GC content: 76.4

Gene sequence:

>1212_bases
ATGACCGAGGATTTCGGCAGTCGCGACGGACAGCGCGACGACAGGACCGGCCCGTCACCCTTCGCCGACATGATCGAGGC
CACCCGCCTGACCGGCCTGGGCCGGCTCGAGGAGGCGACCGCCCTCATCCAGCGCAGCCTCGGGGGTCAGAAGCCCCCGC
CCGCCGATCCGTCGGCCGCGCCGCAGCCTGCCGCCGAGACGCGGAATCCCGCGGCGGAGTCCGGGACGCGGATGCGCGAG
GTGGTGGGGCAGGTCGTGCGCGGGCTCGCGCCGGTGCTGAAGGGCCTGGGCAACCCGCTGCGGCCGCCGGCCCCGCCGCC
CGCCGACCGGGCGGGCGGGACCGGGGGCCGCTTCGTGGCGCTGACCCACGCCGAGCCCGCCGGCACCCGCGAGTACAAGC
TGTTCATCCCGAGCCACCCCGACCGGCCGGCGCCGCTGATCGTGATGCTGCACGGCTGCACGCAGAATCCGGACGATTTC
GCCGCCGGCACCGGCATGAACACCCTCGCCGAGCGCGCCGGCGTGTTCGTGGCCTACCCGGCCCAGAGCCGCCAGGCCCA
CGCCCAGCGCTGCTGGAACTGGTACGAGCCCCGGGACCAGGGCCGCGGCGCGGGCGAGCCGGCCATCATCGCGGGCCTCA
CCCGCGCGATCATGGCGGAGCACGCGATCGACCCGGCCCGGGTCTACATCGCCGGCCTGTCGGCGGGGGGCGCCGCCGCC
CTCAACATCGCCCGCGCCTACCCGGATCTCTACGCCGCCGTCGGCGTGCATTCGGGCCTCGCGGCCGGCTGCGCCCGGGA
TCTCGGCTCGGCGCTGATGGCCATGCAGGTCGGTGCACCGGGCCTCGGCGCTCCGACCGCCTTCGGCACCCCCGCGCCCG
CCGCCCGCGTCCCGACGATCATCTTCCACGGCGAGGACGACGGCACGGTGAGCGTGCGCAACGCCGACCAGGTGCTCGCC
CAGGCCAAGGTCGCCGGGCTGACGCCCCGGTCGGAGACGTTCGAGGGCCGCGGCCATCCCTTCACCCGCACCCGCTACCG
GGACGAGGCGGGCCGGGTCGTGGTCGAGGATTGGCGGGTGCGCGGCGCCGGCCACGCCTGGTCGGGCGGCCGCCCCGACG
GCAGCTACACGGATCCCGAGGGGCCCGACGCGTCCCGGGCGATGCTCGACTTCTTCGCCGAGCACCGGCTGGGACCGGCG
CGGCGCCCGTGA

Upstream 100 bases:

>100_bases
GCGCACCATCTCTCGGGATGGCGGGGGCTGGTCCACGCGGTGGCACGGGCGTGCCGGGTCCGGCCGTGACGGAGATGCTG
AGGCAGTGAGGCGCGCACGC

Downstream 100 bases:

>100_bases
GGGATTTCCGGCTGCCGCGCGCCCCGGCGGGTTGTCAGGGCGCGGCCCTGATGTATCGCAGGGCTCTGCCCTGCCCCCAC
AAAAGGTCCCGGACCTTTTG

Product: PHB depolymerase family esterase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTEDFGSRDGQRDDRTGPSPFADMIEATRLTGLGRLEEATALIQRSLGGQKPPPADPSAAPQPAAETRNPAAESGTRMRE
VVGQVVRGLAPVLKGLGNPLRPPAPPPADRAGGTGGRFVALTHAEPAGTREYKLFIPSHPDRPAPLIVMLHGCTQNPDDF
AAGTGMNTLAERAGVFVAYPAQSRQAHAQRCWNWYEPRDQGRGAGEPAIIAGLTRAIMAEHAIDPARVYIAGLSAGGAAA
LNIARAYPDLYAAVGVHSGLAAGCARDLGSALMAMQVGAPGLGAPTAFGTPAPAARVPTIIFHGEDDGTVSVRNADQVLA
QAKVAGLTPRSETFEGRGHPFTRTRYRDEAGRVVVEDWRVRGAGHAWSGGRPDGSYTDPEGPDASRAMLDFFAEHRLGPA
RRP

Sequences:

>Translated_403_residues
MTEDFGSRDGQRDDRTGPSPFADMIEATRLTGLGRLEEATALIQRSLGGQKPPPADPSAAPQPAAETRNPAAESGTRMRE
VVGQVVRGLAPVLKGLGNPLRPPAPPPADRAGGTGGRFVALTHAEPAGTREYKLFIPSHPDRPAPLIVMLHGCTQNPDDF
AAGTGMNTLAERAGVFVAYPAQSRQAHAQRCWNWYEPRDQGRGAGEPAIIAGLTRAIMAEHAIDPARVYIAGLSAGGAAA
LNIARAYPDLYAAVGVHSGLAAGCARDLGSALMAMQVGAPGLGAPTAFGTPAPAARVPTIIFHGEDDGTVSVRNADQVLA
QAKVAGLTPRSETFEGRGHPFTRTRYRDEAGRVVVEDWRVRGAGHAWSGGRPDGSYTDPEGPDASRAMLDFFAEHRLGPA
RRP
>Mature_402_residues
TEDFGSRDGQRDDRTGPSPFADMIEATRLTGLGRLEEATALIQRSLGGQKPPPADPSAAPQPAAETRNPAAESGTRMREV
VGQVVRGLAPVLKGLGNPLRPPAPPPADRAGGTGGRFVALTHAEPAGTREYKLFIPSHPDRPAPLIVMLHGCTQNPDDFA
AGTGMNTLAERAGVFVAYPAQSRQAHAQRCWNWYEPRDQGRGAGEPAIIAGLTRAIMAEHAIDPARVYIAGLSAGGAAAL
NIARAYPDLYAAVGVHSGLAAGCARDLGSALMAMQVGAPGLGAPTAFGTPAPAARVPTIIFHGEDDGTVSVRNADQVLAQ
AKVAGLTPRSETFEGRGHPFTRTRYRDEAGRVVVEDWRVRGAGHAWSGGRPDGSYTDPEGPDASRAMLDFFAEHRLGPAR
RP

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 42310; Mature: 42179

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEDFGSRDGQRDDRTGPSPFADMIEATRLTGLGRLEEATALIQRSLGGQKPPPADPSAA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
PQPAAETRNPAAESGTRMREVVGQVVRGLAPVLKGLGNPLRPPAPPPADRAGGTGGRFVA
CCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEE
LTHAEPAGTREYKLFIPSHPDRPAPLIVMLHGCTQNPDDFAAGTGMNTLAERAGVFVAYP
EEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCHHHHCCCCHHHHHHHCCEEEEEC
AQSRQAHAQRCWNWYEPRDQGRGAGEPAIIAGLTRAIMAEHAIDPARVYIAGLSAGGAAA
CHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHH
LNIARAYPDLYAAVGVHSGLAAGCARDLGSALMAMQVGAPGLGAPTAFGTPAPAARVPTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEE
IFHGEDDGTVSVRNADQVLAQAKVAGLTPRSETFEGRGHPFTRTRYRDEAGRVVVEDWRV
EEECCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEEE
RGAGHAWSGGRPDGSYTDPEGPDASRAMLDFFAEHRLGPARRP
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEDFGSRDGQRDDRTGPSPFADMIEATRLTGLGRLEEATALIQRSLGGQKPPPADPSAA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
PQPAAETRNPAAESGTRMREVVGQVVRGLAPVLKGLGNPLRPPAPPPADRAGGTGGRFVA
CCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEE
LTHAEPAGTREYKLFIPSHPDRPAPLIVMLHGCTQNPDDFAAGTGMNTLAERAGVFVAYP
EEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCHHHHCCCCHHHHHHHCCEEEEEC
AQSRQAHAQRCWNWYEPRDQGRGAGEPAIIAGLTRAIMAEHAIDPARVYIAGLSAGGAAA
CHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHH
LNIARAYPDLYAAVGVHSGLAAGCARDLGSALMAMQVGAPGLGAPTAFGTPAPAARVPTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEE
IFHGEDDGTVSVRNADQVLAQAKVAGLTPRSETFEGRGHPFTRTRYRDEAGRVVVEDWRV
EEECCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEEE
RGAGHAWSGGRPDGSYTDPEGPDASRAMLDFFAEHRLGPARRP
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]