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The map label for this gene is dmlA [H]

Identifier: 170750390

GI number: 170750390

Start: 4243586

End: 4244677

Strand: Reverse

Name: dmlA [H]

Synonym: Mrad2831_3993

Alternate gene names: 170750390

Gene position: 4244677-4243586 (Counterclockwise)

Preceding gene: 170750391

Following gene: 170750378

Centisome position: 69.84

GC content: 69.32

Gene sequence:

>1092_bases
ATGGCCACTCCGGAGAACAGCCAGCAGCGCCGCTACCGCATCGCGGTGATCCCCGGCGACGGCATCGGCAAGGAGGTGGT
GCCGGAGGGCGTGCGCGTGCTGGAGCAGGCCGCCAAGCGGCACGGCTTCAGCCTCCAGCTCGACTGGTTCGATTTCGCCA
GCTGCGACTACTACGAGCGGCACGGCCGGATGATGCCGGAGGACTGGAAGGCGCGGATCGAGACCCACGACGCGATCTTT
TTCGGCGCCGTCGGCATGCCCGAGCGGGTGCCCGACCACGTCTCCCTCTGGGGCTCGCTGATCCTGTTCCGGCGGGAATT
CGACCAGTACGCCAACCTGCGTCCGGTGCGGCTGATGCCGGGGGTGAAGTGCCCGCTCGCCGACCGGGAGCCCGGCGACA
TCGACTTCTGGGTGGTCCGGGAGAACACCGAGGGCGAGTACTCGAACGCGGGCGGCCGGATGTTCGCCGGCACGGAGCGG
GAATTCGCCCTGCAGGAGACGATCATGACCCGCCACGGGACCGACCGGATCCTCAGGTTCGCCTTCGAGCTGGCCCAGAA
GCGCCCGAAGAAGCACCTGACCTCGGCCACGAAGTCGAACGGCATCTCGATCACCATGCCGTACTGGGACGAGCGGGTGA
AGGCGGTGGCCGGGGCCTATCCGGACGTGCGGTGGGACCAGTACCACATCGACATCCTGACCGCGCATTTCGTCCTCAAC
CCGGACCGGTTCGACGTGGTGGTCGCCTCCAACCTGTTCGGCGACATCCTGTCGGATCTGGGGCCGGCCTGCACGGGCAC
GATCGGCATCGCGCCGTCCGGCAACATCAACCCGGAGCGGGTGCATCCTTCCGTGTTCGAGCCGGTCCACGGCTCGGCGC
CGGACATCGCCGGGCAGGGGATCGCCAACCCGATCGGCCAGATCTGGTCGGGGGCGATGATGCTGGAGCATCTCGGCGAG
CACGCGGCGGCGGCCGAGATCGTCAGCGGGATCGAGCGGGTGCTGTCCGAGCGGACGCTGCGCACCCGCGACCTCGGCGG
CAACGCCGACACGGAGGCCTGCGGCAGGGCGGTGGCGCAGGCGCTGGGGTAG

Upstream 100 bases:

>100_bases
GTCAGGCCGTGGTGTTGACCCGGTGCCGGCGCGGACCAATCACCTGACGCCGGCGACCCGGGCTCCGCACGGGGCCGCCC
TCGAGACGCGTGGAGGATTC

Downstream 100 bases:

>100_bases
GGCAATCACCCCGAGCCCCCTTCCACGCCCTCATCCTGAGGTGCCCGCGCCGGCGGGCCTCGGAGGAGCATTCCAGGGAT
CGCGAGACCTCTGGACGGCT

Product: tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MATPENSQQRRYRIAVIPGDGIGKEVVPEGVRVLEQAAKRHGFSLQLDWFDFASCDYYERHGRMMPEDWKARIETHDAIF
FGAVGMPERVPDHVSLWGSLILFRREFDQYANLRPVRLMPGVKCPLADREPGDIDFWVVRENTEGEYSNAGGRMFAGTER
EFALQETIMTRHGTDRILRFAFELAQKRPKKHLTSATKSNGISITMPYWDERVKAVAGAYPDVRWDQYHIDILTAHFVLN
PDRFDVVVASNLFGDILSDLGPACTGTIGIAPSGNINPERVHPSVFEPVHGSAPDIAGQGIANPIGQIWSGAMMLEHLGE
HAAAAEIVSGIERVLSERTLRTRDLGGNADTEACGRAVAQALG

Sequences:

>Translated_363_residues
MATPENSQQRRYRIAVIPGDGIGKEVVPEGVRVLEQAAKRHGFSLQLDWFDFASCDYYERHGRMMPEDWKARIETHDAIF
FGAVGMPERVPDHVSLWGSLILFRREFDQYANLRPVRLMPGVKCPLADREPGDIDFWVVRENTEGEYSNAGGRMFAGTER
EFALQETIMTRHGTDRILRFAFELAQKRPKKHLTSATKSNGISITMPYWDERVKAVAGAYPDVRWDQYHIDILTAHFVLN
PDRFDVVVASNLFGDILSDLGPACTGTIGIAPSGNINPERVHPSVFEPVHGSAPDIAGQGIANPIGQIWSGAMMLEHLGE
HAAAAEIVSGIERVLSERTLRTRDLGGNADTEACGRAVAQALG
>Mature_362_residues
ATPENSQQRRYRIAVIPGDGIGKEVVPEGVRVLEQAAKRHGFSLQLDWFDFASCDYYERHGRMMPEDWKARIETHDAIFF
GAVGMPERVPDHVSLWGSLILFRREFDQYANLRPVRLMPGVKCPLADREPGDIDFWVVRENTEGEYSNAGGRMFAGTERE
FALQETIMTRHGTDRILRFAFELAQKRPKKHLTSATKSNGISITMPYWDERVKAVAGAYPDVRWDQYHIDILTAHFVLNP
DRFDVVVASNLFGDILSDLGPACTGTIGIAPSGNINPERVHPSVFEPVHGSAPDIAGQGIANPIGQIWSGAMMLEHLGEH
AAAAEIVSGIERVLSERTLRTRDLGGNADTEACGRAVAQALG

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=337, Percent_Identity=33.2344213649852, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI4758582, Length=347, Percent_Identity=29.6829971181556, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI28178838, Length=337, Percent_Identity=29.673590504451, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI28178821, Length=347, Percent_Identity=27.9538904899135, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI28178816, Length=358, Percent_Identity=27.9329608938547, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI28178819, Length=186, Percent_Identity=31.7204301075269, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1788101, Length=358, Percent_Identity=64.5251396648045, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI87081683, Length=348, Percent_Identity=38.7931034482759, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1787381, Length=369, Percent_Identity=28.1842818428184, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71986051, Length=340, Percent_Identity=33.5294117647059, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17550882, Length=350, Percent_Identity=29.4285714285714, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI25144293, Length=357, Percent_Identity=28.0112044817927, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=355, Percent_Identity=27.887323943662, Blast_Score=118, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6322097, Length=362, Percent_Identity=37.5690607734807, Blast_Score=202, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6324709, Length=371, Percent_Identity=31.266846361186, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324291, Length=350, Percent_Identity=30.8571428571429, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6319830, Length=366, Percent_Identity=29.5081967213115, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24643270, Length=366, Percent_Identity=31.1475409836066, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24643268, Length=366, Percent_Identity=31.1475409836066, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24661184, Length=355, Percent_Identity=29.8591549295775, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI281362242, Length=360, Percent_Identity=29.4444444444444, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24648872, Length=360, Percent_Identity=29.4444444444444, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI161078637, Length=256, Percent_Identity=28.90625, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078635, Length=256, Percent_Identity=28.90625, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI20130355, Length=261, Percent_Identity=27.9693486590038, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI161078633, Length=256, Percent_Identity=28.90625, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24650122, Length=256, Percent_Identity=28.90625, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI161078639, Length=255, Percent_Identity=29.0196078431373, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 40293; Mature: 40162

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPENSQQRRYRIAVIPGDGIGKEVVPEGVRVLEQAAKRHGFSLQLDWFDFASCDYYER
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHH
HGRMMPEDWKARIETHDAIFFGAVGMPERVPDHVSLWGSLILFRREFDQYANLRPVRLMP
CCCCCCHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECC
GVKCPLADREPGDIDFWVVRENTEGEYSNAGGRMFAGTEREFALQETIMTRHGTDRILRF
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCHHHHHHH
AFELAQKRPKKHLTSATKSNGISITMPYWDERVKAVAGAYPDVRWDQYHIDILTAHFVLN
HHHHHHHCHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEEC
PDRFDVVVASNLFGDILSDLGPACTGTIGIAPSGNINPERVHPSVFEPVHGSAPDIAGQG
CCCEEEEEHHHHHHHHHHHCCCHHCCEEECCCCCCCCHHHCCCHHHCCCCCCCCCCCCCC
IANPIGQIWSGAMMLEHLGEHAAAAEIVSGIERVLSERTLRTRDLGGNADTEACGRAVAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
ALG
HCC
>Mature Secondary Structure 
ATPENSQQRRYRIAVIPGDGIGKEVVPEGVRVLEQAAKRHGFSLQLDWFDFASCDYYER
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHH
HGRMMPEDWKARIETHDAIFFGAVGMPERVPDHVSLWGSLILFRREFDQYANLRPVRLMP
CCCCCCHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECC
GVKCPLADREPGDIDFWVVRENTEGEYSNAGGRMFAGTEREFALQETIMTRHGTDRILRF
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCHHHHHHH
AFELAQKRPKKHLTSATKSNGISITMPYWDERVKAVAGAYPDVRWDQYHIDILTAHFVLN
HHHHHHHCHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEEC
PDRFDVVVASNLFGDILSDLGPACTGTIGIAPSGNINPERVHPSVFEPVHGSAPDIAGQG
CCCEEEEEHHHHHHHHHHHCCCHHCCEEECCCCCCCCHHHCCCHHHCCCCCCCCCCCCCC
IANPIGQIWSGAMMLEHLGEHAAAAEIVSGIERVLSERTLRTRDLGGNADTEACGRAVAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
ALG
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]