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The map label for this gene is ynfJ [C]

Identifier: 170750391

GI number: 170750391

Start: 4244780

End: 4246711

Strand: Reverse

Name: ynfJ [C]

Synonym: Mrad2831_3994

Alternate gene names: 170750391

Gene position: 4246711-4244780 (Counterclockwise)

Preceding gene: 170750404

Following gene: 170750390

Centisome position: 69.87

GC content: 74.69

Gene sequence:

>1932_bases
GTGAGCGCGCGACAGCCAGCGGCCGCCGCCGGCGGGAACAGGGCGCCCGGCAACGGATCGGCCGACGTGCCGGCCCGATC
GGCCGCCGGGCCGGGCCGGCGGCCGCGCCGGCGCTGGGCGGGGGCTGGCCTCGTCCAGGCCCCCGGACGGCTCCGCAGCC
TTGTCCGCCGGAGCGAGGGCGGCCTCGTCGCCCTCGCGACCCTGGTGGGCTGCGTCGCCGGCGTGGCGGTCTCGGCCATG
ACCTGGATCACCCAGACCCTGCGCGAGCTGCTCTACCAGCTGCCCGCGGGCGCGCGACTCAGCGCCGTCGACGCCGTGTC
GCCGGCGCTCCTGCTCGTGGGCCCGTGCCTCGGCGGCGCCGTCCTCGGCCTGCTGATCGTCGCCGCCAACTACGTCCGCG
GCCCGCGCAGGCGCCCGGCCATCGATCCCATCGAGGCCAACGCCCTGCACGGCGGCCGCATGTCCCTGCGCGACAGCCTC
TACCTCGCCCTCCAGAACGTCATCTCGAACGGGTCCGGCGCCTCGGTGGGCCTCGAAGCCGGCTACACACAGATTGCCTC
CGGCCTCGCCTCGCGGCTGGGCATCGCCTTCGAGGTGCGGCGCAGCGACCTGCGCACCCTGGTGGGCTGCGGCGCGGCCG
GGGCGATCGCGGCCGGCTTCGGGGCGCCGCTCGCCGGCGCGTTCTACGCCTTCGAGCTGATCATCGGCACCTACTCGATC
GCCACGCTGACGCCCGTGGTCGTCGCGGCGCTCTGCGGCAACCTGATCTCGCGCGCCCTGATCGAGGCGCAGCCGCTCGT
CGATCTCGGCGACATGCAGGCGGTCACGACCTCGCACGTCACCAGCCTGGAGATCCTGCCGAGCCTCGCCCTCGGGCTGT
TCTGCGCGGGCCTCGGCATCCTGATCATGCGCGGCGTCACCCTGGTCGAGCGCCTCGTCCAGGCGTCCGGTGTGCCGCGG
GTGGCGGCGCCCGCCTTCGGCGGCCTGTGCGTCGGCGCGCTGGCGCTGCTGGCGACGCCGCAGGTGCTCTCGGGCGGCCA
CGGCGCCCTGCACATGCACTTCGCCGCCGAGGGGCAGGGGCACGGCATCGCGGTCCTGGCCGCGCTGTTCGCCGCCAAGG
CCACGGCCTCGGCGATCTCGATCGGCTCGGGCTTCCGCGGCGGCCTGTTCTTCGCGTCCCTGTTCCTCGGGGCCATCGCC
GGCAAGCTGTTCGCCGCCCTCGCCCCGGTGCTGGTGCCGCCGCTGGCGGATTTCGGCCTGACGCCGCTCGCCTACGCGGT
GATCGGCATGAGCGCCCTCGCGGTGGCGGTCATCGGCGGCCCGCTCACCATGACGTTCCTGGCGCTGGAGGTCACCGGCA
GCCTGCCGATCACCGGCGTCGTCCTGGCGGCTGTGATCGCGTCCTCCCTCACCGTGCGCAAGACCTTCGGCTACTCCTTC
GCCACCTGGCGCTTCCACCTGCGCGGCGAGAGCATCCGCAGCCCCCACGACGTCGGCTGGATCCGCTCGCTGACCGTCGG
CCGGCTGATGCGCCGCAATCCCGGCACCGTGGCGCTGGACACGCCGCTGCCCACCTTCCTGCGCGCCCATCCCCTCGGCT
CGCCCTCCCGGGTGATCGCCGTGGACGGGGGCGGGCGCTACGCCGGCATCCTGAACGTGCCCGAGGCGCACGCGGCCGTC
CGGGATGCCGGCGCCGACGCGCAGCCGGTCCTCGCCGACCTGCTCCTGCACGCGGACGATTTCCTCACGCCGGGCATGAA
CGCCAAGGACGCCGCCGCCGCCTTCGACAGGACCGAGAGCGAGGCGCTCGCGGTCGTCGAGAGCGCCGAGAGCCGGAAGG
TGCTGGGCCTGCTCACCGAGAGCCACACGCTCAAGCGCTACAGCGAGGAGCTCGACCGCCAGCGCCGGGCGATGGCGGGT
GAAACCGACTGA

Upstream 100 bases:

>100_bases
ACCGCCCGTTTGCAACGGGATCGGACCCGGCTGTAACTGCGACGGCGGGGTCGACGCGGCGGCGGCGCCGATCGGACGGA
CCGGAGGCGAGGGGCGGACC

Downstream 100 bases:

>100_bases
TTGTCAGGCCGTGGTGTTGACCCGGTGCCGGCGCGGACCAATCACCTGACGCCGGCGACCCGGGCTCCGCACGGGGCCGC
CCTCGAGACGCGTGGAGGAT

Product: chloride channel core

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MSARQPAAAAGGNRAPGNGSADVPARSAAGPGRRPRRRWAGAGLVQAPGRLRSLVRRSEGGLVALATLVGCVAGVAVSAM
TWITQTLRELLYQLPAGARLSAVDAVSPALLLVGPCLGGAVLGLLIVAANYVRGPRRRPAIDPIEANALHGGRMSLRDSL
YLALQNVISNGSGASVGLEAGYTQIASGLASRLGIAFEVRRSDLRTLVGCGAAGAIAAGFGAPLAGAFYAFELIIGTYSI
ATLTPVVVAALCGNLISRALIEAQPLVDLGDMQAVTTSHVTSLEILPSLALGLFCAGLGILIMRGVTLVERLVQASGVPR
VAAPAFGGLCVGALALLATPQVLSGGHGALHMHFAAEGQGHGIAVLAALFAAKATASAISIGSGFRGGLFFASLFLGAIA
GKLFAALAPVLVPPLADFGLTPLAYAVIGMSALAVAVIGGPLTMTFLALEVTGSLPITGVVLAAVIASSLTVRKTFGYSF
ATWRFHLRGESIRSPHDVGWIRSLTVGRLMRRNPGTVALDTPLPTFLRAHPLGSPSRVIAVDGGGRYAGILNVPEAHAAV
RDAGADAQPVLADLLLHADDFLTPGMNAKDAAAAFDRTESEALAVVESAESRKVLGLLTESHTLKRYSEELDRQRRAMAG
ETD

Sequences:

>Translated_643_residues
MSARQPAAAAGGNRAPGNGSADVPARSAAGPGRRPRRRWAGAGLVQAPGRLRSLVRRSEGGLVALATLVGCVAGVAVSAM
TWITQTLRELLYQLPAGARLSAVDAVSPALLLVGPCLGGAVLGLLIVAANYVRGPRRRPAIDPIEANALHGGRMSLRDSL
YLALQNVISNGSGASVGLEAGYTQIASGLASRLGIAFEVRRSDLRTLVGCGAAGAIAAGFGAPLAGAFYAFELIIGTYSI
ATLTPVVVAALCGNLISRALIEAQPLVDLGDMQAVTTSHVTSLEILPSLALGLFCAGLGILIMRGVTLVERLVQASGVPR
VAAPAFGGLCVGALALLATPQVLSGGHGALHMHFAAEGQGHGIAVLAALFAAKATASAISIGSGFRGGLFFASLFLGAIA
GKLFAALAPVLVPPLADFGLTPLAYAVIGMSALAVAVIGGPLTMTFLALEVTGSLPITGVVLAAVIASSLTVRKTFGYSF
ATWRFHLRGESIRSPHDVGWIRSLTVGRLMRRNPGTVALDTPLPTFLRAHPLGSPSRVIAVDGGGRYAGILNVPEAHAAV
RDAGADAQPVLADLLLHADDFLTPGMNAKDAAAAFDRTESEALAVVESAESRKVLGLLTESHTLKRYSEELDRQRRAMAG
ETD
>Mature_642_residues
SARQPAAAAGGNRAPGNGSADVPARSAAGPGRRPRRRWAGAGLVQAPGRLRSLVRRSEGGLVALATLVGCVAGVAVSAMT
WITQTLRELLYQLPAGARLSAVDAVSPALLLVGPCLGGAVLGLLIVAANYVRGPRRRPAIDPIEANALHGGRMSLRDSLY
LALQNVISNGSGASVGLEAGYTQIASGLASRLGIAFEVRRSDLRTLVGCGAAGAIAAGFGAPLAGAFYAFELIIGTYSIA
TLTPVVVAALCGNLISRALIEAQPLVDLGDMQAVTTSHVTSLEILPSLALGLFCAGLGILIMRGVTLVERLVQASGVPRV
AAPAFGGLCVGALALLATPQVLSGGHGALHMHFAAEGQGHGIAVLAALFAAKATASAISIGSGFRGGLFFASLFLGAIAG
KLFAALAPVLVPPLADFGLTPLAYAVIGMSALAVAVIGGPLTMTFLALEVTGSLPITGVVLAAVIASSLTVRKTFGYSFA
TWRFHLRGESIRSPHDVGWIRSLTVGRLMRRNPGTVALDTPLPTFLRAHPLGSPSRVIAVDGGGRYAGILNVPEAHAAVR
DAGADAQPVLADLLLHADDFLTPGMNAKDAAAAFDRTESEALAVVESAESRKVLGLLTESHTLKRYSEELDRQRRAMAGE
TD

Specific function: Unknown

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081943, Length=411, Percent_Identity=28.4671532846715, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 65782; Mature: 65651

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARQPAAAAGGNRAPGNGSADVPARSAAGPGRRPRRRWAGAGLVQAPGRLRSLVRRSEG
CCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCC
GLVALATLVGCVAGVAVSAMTWITQTLRELLYQLPAGARLSAVDAVSPALLLVGPCLGGA
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHH
VLGLLIVAANYVRGPRRRPAIDPIEANALHGGRMSLRDSLYLALQNVISNGSGASVGLEA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHH
GYTQIASGLASRLGIAFEVRRSDLRTLVGCGAAGAIAAGFGAPLAGAFYAFELIIGTYSI
HHHHHHHHHHHHHCHHHEEHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ATLTPVVVAALCGNLISRALIEAQPLVDLGDMQAVTTSHVTSLEILPSLALGLFCAGLGI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIMRGVTLVERLVQASGVPRVAAPAFGGLCVGALALLATPQVLSGGHGALHMHFAAEGQG
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCEEEEEEECCCCC
HGIAVLAALFAAKATASAISIGSGFRGGLFFASLFLGAIAGKLFAALAPVLVPPLADFGL
CHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
TPLAYAVIGMSALAVAVIGGPLTMTFLALEVTGSLPITGVVLAAVIASSLTVRKTFGYSF
HHHHHHHHHHHHHHHHHHCCHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCE
ATWRFHLRGESIRSPHDVGWIRSLTVGRLMRRNPGTVALDTPLPTFLRAHPLGSPSRVIA
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHCCCCCCCEEEE
VDGGGRYAGILNVPEAHAAVRDAGADAQPVLADLLLHADDFLTPGMNAKDAAAAFDRTES
ECCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCH
EALAVVESAESRKVLGLLTESHTLKRYSEELDRQRRAMAGETD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SARQPAAAAGGNRAPGNGSADVPARSAAGPGRRPRRRWAGAGLVQAPGRLRSLVRRSEG
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCC
GLVALATLVGCVAGVAVSAMTWITQTLRELLYQLPAGARLSAVDAVSPALLLVGPCLGGA
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHH
VLGLLIVAANYVRGPRRRPAIDPIEANALHGGRMSLRDSLYLALQNVISNGSGASVGLEA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHH
GYTQIASGLASRLGIAFEVRRSDLRTLVGCGAAGAIAAGFGAPLAGAFYAFELIIGTYSI
HHHHHHHHHHHHHCHHHEEHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ATLTPVVVAALCGNLISRALIEAQPLVDLGDMQAVTTSHVTSLEILPSLALGLFCAGLGI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIMRGVTLVERLVQASGVPRVAAPAFGGLCVGALALLATPQVLSGGHGALHMHFAAEGQG
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCEEEEEEECCCCC
HGIAVLAALFAAKATASAISIGSGFRGGLFFASLFLGAIAGKLFAALAPVLVPPLADFGL
CHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
TPLAYAVIGMSALAVAVIGGPLTMTFLALEVTGSLPITGVVLAAVIASSLTVRKTFGYSF
HHHHHHHHHHHHHHHHHHCCHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCE
ATWRFHLRGESIRSPHDVGWIRSLTVGRLMRRNPGTVALDTPLPTFLRAHPLGSPSRVIA
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHCCCCCCCEEEE
VDGGGRYAGILNVPEAHAAVRDAGADAQPVLADLLLHADDFLTPGMNAKDAAAAFDRTES
ECCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCH
EALAVVESAESRKVLGLLTESHTLKRYSEELDRQRRAMAGETD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]