Click here to switch to the map view.

The map label for this gene is xapA [H]

Identifier: 170750322

GI number: 170750322

Start: 4175713

End: 4176537

Strand: Reverse

Name: xapA [H]

Synonym: Mrad2831_3925

Alternate gene names: 170750322

Gene position: 4176537-4175713 (Counterclockwise)

Preceding gene: 170750323

Following gene: 170750321

Centisome position: 68.72

GC content: 76.97

Gene sequence:

>825_bases
GTGAGTGAGGCGGCCGCCCGCGACGCCCTGCGCGCCGCCGGCTTCGCGGGTCCCTACGCCTGCGCGGTGGTGACCGGCAC
CGGCCTCGGCGGGATCGCGGCGGCCCTCACGGACGCGAGCGCCGTCGACTACGCGGCGATCCCCGGGTTTCCGGGGCCCG
GCGTCAGCGGCCACGGCGGCCGGCTGCACCGGGGACGCCTCGCGGGGCGACCGGTCCTGATCTTCGAGGGGCGGGCCCAC
GCCTACGAGCGCGGTGACCCGGCGGCGATGCGGGTGCCGCTCGCCTGCGCCCGGCACCTCGGCGCCCGGCGCCTGCTGCT
CACCAACGCCTCCGGATCGCTGCGGCCGGCGACCGGGCCCGGCAGCCTCGTGCTGCTCGCCGACCACATCAACCTCTCCG
GGATGAACCCGCTGATCGGCGAGGCCAGCGACGCGCGGTTCGTGCCGATGACCGAGGCCTACGACCCGGACCTGCGGGCG
CGGCTCCGCGCGGCGGCCGGGGCCTGCGGTGTGTCCCTGGGGGAGGGGATCTACGCGTGGTTCTCGGGCCCGAGCTTCGA
GACGCCCGCGGAGGTGCGGATGGCCGGCCTCCTGGGCGCCGACCTCGTGGGCATGTCGACCGTGCCCGAGGTGATCCTCG
CCCGGTTTCTCGGCCTGCCGGTGGCGGCGGTCTCGGTGGTGACGAACCTCGCGGCCGGCATCGCGGGCGGCGACCCGAAC
CACGCGGAGACGAAGGCGGTGGCGGCCCGGGCCGCGGAGGATCTCGGCCGCCTGGTCCGGGCCTTCGTGGCCGGCCTGCC
CGACGGAGAGACAGCCGATGACTGA

Upstream 100 bases:

>100_bases
GGGCCGGACACCCCGATCCACACCGCCGGTCCGGGCGGCGTCGCCCGCAGCTTCACCCTGGCGGAGCTGCTGCCGGAATC
CTTCGGCCCGGAGACGCTCG

Downstream 100 bases:

>100_bases
TCCCGATGCCGGCGCGGTCGCCCGCCGGGCCCTGCCGCTCCTCGACCTCACCGATCTCGGCGAGACCTGCACCGAGACCA
AGATCGACGTCCTCTGCCGG

Product: purine nucleoside phosphorylase

Products: purine; alpha-D-ribose 1-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSEAAARDALRAAGFAGPYACAVVTGTGLGGIAAALTDASAVDYAAIPGFPGPGVSGHGGRLHRGRLAGRPVLIFEGRAH
AYERGDPAAMRVPLACARHLGARRLLLTNASGSLRPATGPGSLVLLADHINLSGMNPLIGEASDARFVPMTEAYDPDLRA
RLRAAAGACGVSLGEGIYAWFSGPSFETPAEVRMAGLLGADLVGMSTVPEVILARFLGLPVAAVSVVTNLAAGIAGGDPN
HAETKAVAARAAEDLGRLVRAFVAGLPDGETADD

Sequences:

>Translated_274_residues
MSEAAARDALRAAGFAGPYACAVVTGTGLGGIAAALTDASAVDYAAIPGFPGPGVSGHGGRLHRGRLAGRPVLIFEGRAH
AYERGDPAAMRVPLACARHLGARRLLLTNASGSLRPATGPGSLVLLADHINLSGMNPLIGEASDARFVPMTEAYDPDLRA
RLRAAAGACGVSLGEGIYAWFSGPSFETPAEVRMAGLLGADLVGMSTVPEVILARFLGLPVAAVSVVTNLAAGIAGGDPN
HAETKAVAARAAEDLGRLVRAFVAGLPDGETADD
>Mature_273_residues
SEAAARDALRAAGFAGPYACAVVTGTGLGGIAAALTDASAVDYAAIPGFPGPGVSGHGGRLHRGRLAGRPVLIFEGRAHA
YERGDPAAMRVPLACARHLGARRLLLTNASGSLRPATGPGSLVLLADHINLSGMNPLIGEASDARFVPMTEAYDPDLRAR
LRAAAGACGVSLGEGIYAWFSGPSFETPAEVRMAGLLGADLVGMSTVPEVILARFLGLPVAAVSVVTNLAAGIAGGDPNH
AETKAVAARAAEDLGRLVRAFVAGLPDGETADD

Specific function: The nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the nucleoside molecule, with the formation of the corresponding free bases and pentose-1-phosphate. This protein can degrade all purine nucleosides except ade

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=256, Percent_Identity=42.1875, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI47132622, Length=139, Percent_Identity=33.0935251798561, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1788746, Length=245, Percent_Identity=42.4489795918367, Blast_Score=189, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17541190, Length=254, Percent_Identity=35.8267716535433, Blast_Score=153, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6323238, Length=270, Percent_Identity=35.9259259259259, Blast_Score=148, Evalue=8e-37,
Organism=Drosophila melanogaster, GI45552887, Length=255, Percent_Identity=35.6862745098039, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24656093, Length=255, Percent_Identity=35.6862745098039, Blast_Score=143, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24656090, Length=255, Percent_Identity=35.6862745098039, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI45552885, Length=255, Percent_Identity=35.6862745098039, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24762376, Length=261, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI221459247, Length=247, Percent_Identity=27.1255060728745, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR010943
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: 2.4.2.1

Molecular weight: Translated: 27637; Mature: 27506

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAAARDALRAAGFAGPYACAVVTGTGLGGIAAALTDASAVDYAAIPGFPGPGVSGHGG
CCHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCC
RLHRGRLAGRPVLIFEGRAHAYERGDPAAMRVPLACARHLGARRLLLTNASGSLRPATGP
CEECCCCCCCCEEEECCCCCCCCCCCCHHEEHHHHHHHHCCCCEEEEECCCCCCCCCCCC
GSLVLLADHINLSGMNPLIGEASDARFVPMTEAYDPDLRARLRAAAGACGVSLGEGIYAW
CCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCHHCCCEEEE
FSGPSFETPAEVRMAGLLGADLVGMSTVPEVILARFLGLPVAAVSVVTNLAAGIAGGDPN
ECCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCC
HAETKAVAARAAEDLGRLVRAFVAGLPDGETADD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SEAAARDALRAAGFAGPYACAVVTGTGLGGIAAALTDASAVDYAAIPGFPGPGVSGHGG
CHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCC
RLHRGRLAGRPVLIFEGRAHAYERGDPAAMRVPLACARHLGARRLLLTNASGSLRPATGP
CEECCCCCCCCEEEECCCCCCCCCCCCHHEEHHHHHHHHCCCCEEEEECCCCCCCCCCCC
GSLVLLADHINLSGMNPLIGEASDARFVPMTEAYDPDLRARLRAAAGACGVSLGEGIYAW
CCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCHHCCCEEEE
FSGPSFETPAEVRMAGLLGADLVGMSTVPEVILARFLGLPVAAVSVVTNLAAGIAGGDPN
ECCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCC
HAETKAVAARAAEDLGRLVRAFVAGLPDGETADD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: purine nucleoside; phosphate

Specific reaction: purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559336; 9205837; 9278503 [H]