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The map label for this gene is purF [H]

Identifier: 170750291

GI number: 170750291

Start: 4140746

End: 4142221

Strand: Reverse

Name: purF [H]

Synonym: Mrad2831_3894

Alternate gene names: 170750291

Gene position: 4142221-4140746 (Counterclockwise)

Preceding gene: 170750292

Following gene: 170750290

Centisome position: 68.15

GC content: 69.72

Gene sequence:

>1476_bases
ATGTCACACGCCGCCGCCTCCACCGACACCTTCGATCTCGATCTCGACGGCGACACGCTGCGCGAGGAATGCGGGGTCTT
CGGCATCTTCGGCCATCCGGACGCCTCGGCGATCGTCGCGCTGGGCCTCCACGCCCTGCAGCACCGGGGCCAGGAGGCGG
CCGGCATCGTCTCGTTCGACGGCTCGGTGTTCCATTCCGAGCGCCGCCCCGGCCTCGTGGGCGATTCCTTCTCGGACGGC
GACACGATCGCCCGCCTCAAGGGCCGCGCCGCCATCGGCCACGTGCGCTACTCGACCACCGGCGGCACGATCCTGCGCAA
CGTCCAGCCGCTCTTCGCGGAGCTCGCCAGCGGCGGCCTCGCGGTCGCCCACAACGGCAACCTCACCAACGCCCTGTCGA
TCCGCCGCGACCTCGTGCGCGACGGCGCGATCACGCAGTCGACCTCCGACACCGAGGTGATCCTGCACCTCGCGGCCCGC
TCGCGGAAGCCCCGGATCATCGAGCGCTTCATCGACGCCCTGCAGGCGATCCAGGGCGCCTACGCGATCGTGGCGCTGAC
CAACAAGAAGCTGATCGGCGCCCGCGACCCGCTGGGCATCCGCCCCCTGGTGCTGGGCGAGCTCGACGGCCGCTACATGC
TGGCCTCCGAGACCTGCGCCCTCGACATCATCGGCGCCCGGTTCGTGCGCGACATCGAGAACGGCGAGATCGTGGTGATC
TCCGAGGAGGGGATCGAGTCGATCCGCTTCGCCGACGCCGTGCCGATGCGGCCCTGCATCTTCGAGTACATCTACTTCGC
CCGGCCGGATTCGGTGGTGAACGGCAAGAGCGTCTACGCGGTGCGCAAGGCGATCGGCCGGGAGCTGGCCCGCGAGGCCT
CGGTCGCGGCCGACATCGTCGTGCCGGTGCCGGATTCCGGCGTGCCGGCGGCGCTCGGCTTCTCGCAGGAGACCGGGATC
CCGTTCGAGATGGGGATCATCCGCAACCACTACGTCGGCCGGACATTCATCCAGCCGACCCAGTCGGTGCGCGAGCTCGG
CGTGCGCATGAAGCACTCGGCCAACCGCGCGGCGATCGAGGGCAAGAGCATCGTCCTCGTGGACGACAGCCTCGTGCGCG
GCACGACCTCGGTGAAGATCGTCCGGATGATGCGCGAGGCCGGCGCCGCGGAGGTCCATTTCCGGATCGCGTCGCCGCCG
ATCACCTACCCGGACTTCTACGGGATCGACACGCCCGAGCGGGAGAAGCTGCTCGCCGCGACCCACGATCTCGAGGCCAT
GCGCAAGTATATCGGCGCCGATTCGCTGGCCTTCCTGTCGATCCCCGGCCTCTACCGGGCGATGGGCGAGGAGGCGCGGA
ACGCGGCCTGCCCGCAATACACCGACCACTGCTTCACGGGCGACTACCCGACCGGCCTGACCGACCTGTCCATCGCTGGC
CCGAAGCGCTTCGCCATGCTGGCCGAGGCCGACTGA

Upstream 100 bases:

>100_bases
GGAACGCCTAGATAGCGGTTCCGGTGGTGTACGGATTGCTTCCGCGACGACCGGCGCGCCGGCCCGTCGCCGCCGGGATC
TGTGAGTGATCGACAGCCCC

Downstream 100 bases:

>100_bases
CAGGATCCGGCCGTTGCGCGCGAACGGGCATCCCCTGTGCGCGGAAATGCGCTAGGTCCTCGCCATGGCTGAGTCTCAGA
GAACCCTGGAAGGCCGCGTC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSHAAASTDTFDLDLDGDTLREECGVFGIFGHPDASAIVALGLHALQHRGQEAAGIVSFDGSVFHSERRPGLVGDSFSDG
DTIARLKGRAAIGHVRYSTTGGTILRNVQPLFAELASGGLAVAHNGNLTNALSIRRDLVRDGAITQSTSDTEVILHLAAR
SRKPRIIERFIDALQAIQGAYAIVALTNKKLIGARDPLGIRPLVLGELDGRYMLASETCALDIIGARFVRDIENGEIVVI
SEEGIESIRFADAVPMRPCIFEYIYFARPDSVVNGKSVYAVRKAIGRELAREASVAADIVVPVPDSGVPAALGFSQETGI
PFEMGIIRNHYVGRTFIQPTQSVRELGVRMKHSANRAAIEGKSIVLVDDSLVRGTTSVKIVRMMREAGAAEVHFRIASPP
ITYPDFYGIDTPEREKLLAATHDLEAMRKYIGADSLAFLSIPGLYRAMGEEARNAACPQYTDHCFTGDYPTGLTDLSIAG
PKRFAMLAEAD

Sequences:

>Translated_491_residues
MSHAAASTDTFDLDLDGDTLREECGVFGIFGHPDASAIVALGLHALQHRGQEAAGIVSFDGSVFHSERRPGLVGDSFSDG
DTIARLKGRAAIGHVRYSTTGGTILRNVQPLFAELASGGLAVAHNGNLTNALSIRRDLVRDGAITQSTSDTEVILHLAAR
SRKPRIIERFIDALQAIQGAYAIVALTNKKLIGARDPLGIRPLVLGELDGRYMLASETCALDIIGARFVRDIENGEIVVI
SEEGIESIRFADAVPMRPCIFEYIYFARPDSVVNGKSVYAVRKAIGRELAREASVAADIVVPVPDSGVPAALGFSQETGI
PFEMGIIRNHYVGRTFIQPTQSVRELGVRMKHSANRAAIEGKSIVLVDDSLVRGTTSVKIVRMMREAGAAEVHFRIASPP
ITYPDFYGIDTPEREKLLAATHDLEAMRKYIGADSLAFLSIPGLYRAMGEEARNAACPQYTDHCFTGDYPTGLTDLSIAG
PKRFAMLAEAD
>Mature_490_residues
SHAAASTDTFDLDLDGDTLREECGVFGIFGHPDASAIVALGLHALQHRGQEAAGIVSFDGSVFHSERRPGLVGDSFSDGD
TIARLKGRAAIGHVRYSTTGGTILRNVQPLFAELASGGLAVAHNGNLTNALSIRRDLVRDGAITQSTSDTEVILHLAARS
RKPRIIERFIDALQAIQGAYAIVALTNKKLIGARDPLGIRPLVLGELDGRYMLASETCALDIIGARFVRDIENGEIVVIS
EEGIESIRFADAVPMRPCIFEYIYFARPDSVVNGKSVYAVRKAIGRELAREASVAADIVVPVPDSGVPAALGFSQETGIP
FEMGIIRNHYVGRTFIQPTQSVRELGVRMKHSANRAAIEGKSIVLVDDSLVRGTTSVKIVRMMREAGAAEVHFRIASPPI
TYPDFYGIDTPEREKLLAATHDLEAMRKYIGADSLAFLSIPGLYRAMGEEARNAACPQYTDHCFTGDYPTGLTDLSIAGP
KRFAMLAEAD

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=516, Percent_Identity=38.1782945736434, Blast_Score=348, Evalue=4e-96,
Organism=Escherichia coli, GI1788651, Length=475, Percent_Identity=40.2105263157895, Blast_Score=318, Evalue=7e-88,
Organism=Escherichia coli, GI1790167, Length=228, Percent_Identity=29.8245614035088, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=37.160751565762, Blast_Score=308, Evalue=6e-84,
Organism=Saccharomyces cerevisiae, GI6323958, Length=482, Percent_Identity=35.6846473029046, Blast_Score=255, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24659598, Length=495, Percent_Identity=40.4040404040404, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI28573187, Length=495, Percent_Identity=41.010101010101, Blast_Score=353, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24659604, Length=467, Percent_Identity=41.1134903640257, Blast_Score=346, Evalue=3e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52948; Mature: 52817

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHAAASTDTFDLDLDGDTLREECGVFGIFGHPDASAIVALGLHALQHRGQEAAGIVSFD
CCCCCCCCCEEEEECCCHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEC
GSVFHSERRPGLVGDSFSDGDTIARLKGRAAIGHVRYSTTGGTILRNVQPLFAELASGGL
CCEEECCCCCCCCCCCCCCCCHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCE
AVAHNGNLTNALSIRRDLVRDGAITQSTSDTEVILHLAARSRKPRIIERFIDALQAIQGA
EEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHEEHHCCCCCHHHHHHHHHHHHHCCC
YAIVALTNKKLIGARDPLGIRPLVLGELDGRYMLASETCALDIIGARFVRDIENGEIVVI
EEEEEEECCCEECCCCCCCCCCEEEECCCCCEEEECCCCEEEHHHHHHHHHCCCCCEEEE
SEEGIESIRFADAVPMRPCIFEYIYFARPDSVVNGKSVYAVRKAIGRELAREASVAADIV
ECCCCCHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEE
VPVPDSGVPAALGFSQETGIPFEMGIIRNHYVGRTFIQPTQSVRELGVRMKHSANRAAIE
EECCCCCCCCEECCCCCCCCCEEECHHHCCCCCCHHCCHHHHHHHHHHHHHCCCCCEEEC
GKSIVLVDDSLVRGTTSVKIVRMMREAGAAEVHFRIASPPITYPDFYGIDTPEREKLLAA
CCEEEEECCHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHH
THDLEAMRKYIGADSLAFLSIPGLYRAMGEEARNAACPQYTDHCFTGDYPTGLTDLSIAG
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCC
PKRFAMLAEAD
CCCEEEEECCC
>Mature Secondary Structure 
SHAAASTDTFDLDLDGDTLREECGVFGIFGHPDASAIVALGLHALQHRGQEAAGIVSFD
CCCCCCCCEEEEECCCHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEC
GSVFHSERRPGLVGDSFSDGDTIARLKGRAAIGHVRYSTTGGTILRNVQPLFAELASGGL
CCEEECCCCCCCCCCCCCCCCHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCE
AVAHNGNLTNALSIRRDLVRDGAITQSTSDTEVILHLAARSRKPRIIERFIDALQAIQGA
EEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHEEHHCCCCCHHHHHHHHHHHHHCCC
YAIVALTNKKLIGARDPLGIRPLVLGELDGRYMLASETCALDIIGARFVRDIENGEIVVI
EEEEEEECCCEECCCCCCCCCCEEEECCCCCEEEECCCCEEEHHHHHHHHHCCCCCEEEE
SEEGIESIRFADAVPMRPCIFEYIYFARPDSVVNGKSVYAVRKAIGRELAREASVAADIV
ECCCCCHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEE
VPVPDSGVPAALGFSQETGIPFEMGIIRNHYVGRTFIQPTQSVRELGVRMKHSANRAAIE
EECCCCCCCCEECCCCCCCCCEEECHHHCCCCCCHHCCHHHHHHHHHHHHHCCCCCEEEC
GKSIVLVDDSLVRGTTSVKIVRMMREAGAAEVHFRIASPPITYPDFYGIDTPEREKLLAA
CCEEEEECCHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHH
THDLEAMRKYIGADSLAFLSIPGLYRAMGEEARNAACPQYTDHCFTGDYPTGLTDLSIAG
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCC
PKRFAMLAEAD
CCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA