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The map label for this gene is phnJ [H]

Identifier: 170750252

GI number: 170750252

Start: 4104039

End: 4104926

Strand: Reverse

Name: phnJ [H]

Synonym: Mrad2831_3854

Alternate gene names: 170750252

Gene position: 4104926-4104039 (Counterclockwise)

Preceding gene: 170750253

Following gene: 170750251

Centisome position: 67.54

GC content: 70.72

Gene sequence:

>888_bases
ATGAGCGCCGCGATCGCGCACCGCCCGGAGAGCGAACCGGGCTACAACTTCGCCTATCTCGACGAGGGCACGAAGCGGAT
GATCCGCCGGGCCATCCTCAAGGCCATCGCGGTGCCGGGCTATCAGGTGCCCTTCGCCTCCCGCGAGATGCCGATGCCGT
ACGGCTGGGGCACCGGCGGCGTGCAGGTCACGGCCGCGATCCTCGGCCGCTCGGACGTGCTCAAGGTCATCGACCAGGGC
GCCGACGACACCACCAACGCGGTCTCGATCCGCCGCTTCTTCGCCAAGACCTCCGGGGTGGCCACCACGACCCGCACCGC
GGAGGCGACGGTCATCCAGACCCGCCACCGCATCCCCGAGACGCCGCTCACCGAGGGGCAGACGCTGGTCTACCAAGTGC
CGATCCCTGAGCCCCTGCGCTTCCTCGAGCCCCGCGAGACCGAGACGCGCCAGCTCCACGCGCTGGCCGAGTACGGCGCC
ATGCACGTGAAGCTCTACGAGGACATCAGCCGCCACGGCCACATCGCCACCACCTACGCGTACCCGGTGGAGGTGGCGGG
CCGCTACGTGATGGACCCGTCGCCGACGCCGAAATTCGACAACCCCAAGATGGACGACTGCCCGGCGCTCCAGCTCTTCG
GCGCGGGCCGCGAGCGGCGGATCTACGCGGTGCCGCCCCACACGCGGGTGCGCAGCCTCGACTTCGAGGACCACCCGTTC
CGGGTGCAGCACTTCGACCAGCCCTGCGCCCTGTGCGGCGCCGAGGGCGTCTATCTCGACGAGGTGCTGCTCGACGATGC
CGGAAGGCGGATGTTCGTCTGCTCGGACACCGACCACTGCGAGGGGCGCCGCGCGGACGGCCATGTCGGCGCGGGGGACG
CGGCATGA

Upstream 100 bases:

>100_bases
CCCACTACGTCGATTTCCAGGCCGAGCTGGAACTTGTTCGCCGGCTACGCGCCGAGTTCGAGCGCGCGTCCGACGCCGTC
GAGATGCAGGAGGCCGCCGA

Downstream 100 bases:

>100_bases
GTGGCGATGTGAGGCGCGGCATGAGCGACTTCACGGTCCGCCGGGCCGGGAAACCGGACCTGCCCGCGATCCTCGACCTC
TATGCCGAGCTGAACGGCGG

Product: phosphonate metabolism PhnJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSAAIAHRPESEPGYNFAYLDEGTKRMIRRAILKAIAVPGYQVPFASREMPMPYGWGTGGVQVTAAILGRSDVLKVIDQG
ADDTTNAVSIRRFFAKTSGVATTTRTAEATVIQTRHRIPETPLTEGQTLVYQVPIPEPLRFLEPRETETRQLHALAEYGA
MHVKLYEDISRHGHIATTYAYPVEVAGRYVMDPSPTPKFDNPKMDDCPALQLFGAGRERRIYAVPPHTRVRSLDFEDHPF
RVQHFDQPCALCGAEGVYLDEVLLDDAGRRMFVCSDTDHCEGRRADGHVGAGDAA

Sequences:

>Translated_295_residues
MSAAIAHRPESEPGYNFAYLDEGTKRMIRRAILKAIAVPGYQVPFASREMPMPYGWGTGGVQVTAAILGRSDVLKVIDQG
ADDTTNAVSIRRFFAKTSGVATTTRTAEATVIQTRHRIPETPLTEGQTLVYQVPIPEPLRFLEPRETETRQLHALAEYGA
MHVKLYEDISRHGHIATTYAYPVEVAGRYVMDPSPTPKFDNPKMDDCPALQLFGAGRERRIYAVPPHTRVRSLDFEDHPF
RVQHFDQPCALCGAEGVYLDEVLLDDAGRRMFVCSDTDHCEGRRADGHVGAGDAA
>Mature_294_residues
SAAIAHRPESEPGYNFAYLDEGTKRMIRRAILKAIAVPGYQVPFASREMPMPYGWGTGGVQVTAAILGRSDVLKVIDQGA
DDTTNAVSIRRFFAKTSGVATTTRTAEATVIQTRHRIPETPLTEGQTLVYQVPIPEPLRFLEPRETETRQLHALAEYGAM
HVKLYEDISRHGHIATTYAYPVEVAGRYVMDPSPTPKFDNPKMDDCPALQLFGAGRERRIYAVPPHTRVRSLDFEDHPFR
VQHFDQPCALCGAEGVYLDEVLLDDAGRRMFVCSDTDHCEGRRADGHVGAGDAA

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=267, Percent_Identity=79.7752808988764, Blast_Score=463, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 32699; Mature: 32568

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAIAHRPESEPGYNFAYLDEGTKRMIRRAILKAIAVPGYQVPFASREMPMPYGWGTGG
CCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
VQVTAAILGRSDVLKVIDQGADDTTNAVSIRRFFAKTSGVATTTRTAEATVIQTRHRIPE
CEEEEEHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHEEHHHHCCCC
TPLTEGQTLVYQVPIPEPLRFLEPRETETRQLHALAEYGAMHVKLYEDISRHGHIATTYA
CCCCCCCEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHCCEEEEEHHHHHHCCCEEEEEE
YPVEVAGRYVMDPSPTPKFDNPKMDDCPALQLFGAGRERRIYAVPPHTRVRSLDFEDHPF
CCHHHCCEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCEEEECCCCCCEEECCCCCCCE
RVQHFDQPCALCGAEGVYLDEVLLDDAGRRMFVCSDTDHCEGRRADGHVGAGDAA
EEEECCCCHHHHCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAAIAHRPESEPGYNFAYLDEGTKRMIRRAILKAIAVPGYQVPFASREMPMPYGWGTGG
CCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
VQVTAAILGRSDVLKVIDQGADDTTNAVSIRRFFAKTSGVATTTRTAEATVIQTRHRIPE
CEEEEEHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHEEHHHHCCCC
TPLTEGQTLVYQVPIPEPLRFLEPRETETRQLHALAEYGAMHVKLYEDISRHGHIATTYA
CCCCCCCEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHCCEEEEEHHHHHHCCCEEEEEE
YPVEVAGRYVMDPSPTPKFDNPKMDDCPALQLFGAGRERRIYAVPPHTRVRSLDFEDHPF
CCHHHCCEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCEEEECCCCCCEEECCCCCCCE
RVQHFDQPCALCGAEGVYLDEVLLDDAGRRMFVCSDTDHCEGRRADGHVGAGDAA
EEEECCCCHHHHCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]