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The map label for this gene is phnI [H]

Identifier: 170750253

GI number: 170750253

Start: 4104923

End: 4106029

Strand: Reverse

Name: phnI [H]

Synonym: Mrad2831_3855

Alternate gene names: 170750253

Gene position: 4106029-4104923 (Counterclockwise)

Preceding gene: 170750254

Following gene: 170750252

Centisome position: 67.56

GC content: 73.26

Gene sequence:

>1107_bases
ATGTACGTGGCCGTGAAGGGCGGCGAGGCCGCCATCGACAACGCCCACCGGCTCCTCGCCGAGACGCGCCGGGGCGACCC
CGCATTGCCCGAACTCTCCGTGGCGCAGATCCGGGAGCAGATGGCGCTGCTCGTCGACCGGGTCATGACCGAGGGCTCGC
TCTACGATCCGGACCTCGCGGCGCTGGCGCTGAAGCAGGCGCGGGGCGACGTGGTCGAGGCGGTGTTCCTCGTCCGCGCC
TATCGGACGACGCTGCCGCGCTTCGGCGCCTCCGCGCCGGTCGACACCGGGGCCATGGCGGTCGAGCGCCGGATCTCCGC
GACCTTCAAGGACCTTCCGGGCGGGCAGGTGCTCGGGCCGACCTTCGACTACACCCACCGGCTGGTCGACTTCGCGCTCG
CCGCCGAGACCGCGTCCGAGCCCGCCGCCGAGGCCGAGCCGCGCCCCGATGCCGGCACCTGCCCGCGGGTCACCGACCTG
CTGGCCCGGGACGGCCTGATCGAGCCCTCGCCCCCGGAGGACGGCGCCCCCGCGGGCGACCTCACCCGCGAGCCGGTGGA
TTATCCCGCCGACCGGGCCCTGCGCCTCCAGGCGCTCGCCCGGGGCGACGAGGGCTTCGTGCTCGGCCTCGCCTACTCGA
CGCAGCGCGGCTACGGCCGCACCCACCCGTTCGTCGGCGAGATCCGGGTCGGCGAGGTCGCGGTCGAGTTCGTGCCCGAG
GAGCTGGGCTTCGCGGTGCCGCTCGGCCGCGTGCGGCTCACCGAGTGCCAGATGGTCAACCAGTTCAAGGGCACGGGGAC
GGCGCCGCCGCAATTCACCCGCGGCTACGGGCTGAGCTTCGGGCAGAGCGAGCGCAAGGCCATGGCGATGGCGCTCGTGG
ATCGGGCGCTGCGCGCGGAGGAGCTGGGCGAGCCGGTGGCGAGCCCGGCCCAGGACCAGGAATTCGTCCTGGCCCACTGC
GACAGTCTCCAGGCGACCGGCTTTGTGGAGCACCTGAAGCTGCCCCACTACGTCGATTTCCAGGCCGAGCTGGAACTTGT
TCGCCGGCTACGCGCCGAGTTCGAGCGCGCGTCCGACGCCGTCGAGATGCAGGAGGCCGCCGAATGA

Upstream 100 bases:

>100_bases
GAACCGCGCCGGCTTTCCCCTGGGCGTCGACCTGATCCTCACCGCGCCCGGCCGCGTGGCCGGTCTCCCGCGCTCCACGA
CGGTCACGGAGGCCTGACCC

Downstream 100 bases:

>100_bases
GCGCCGCGATCGCGCACCGCCCGGAGAGCGAACCGGGCTACAACTTCGCCTATCTCGACGAGGGCACGAAGCGGATGATC
CGCCGGGCCATCCTCAAGGC

Product: phosphonate metabolism

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MYVAVKGGEAAIDNAHRLLAETRRGDPALPELSVAQIREQMALLVDRVMTEGSLYDPDLAALALKQARGDVVEAVFLVRA
YRTTLPRFGASAPVDTGAMAVERRISATFKDLPGGQVLGPTFDYTHRLVDFALAAETASEPAAEAEPRPDAGTCPRVTDL
LARDGLIEPSPPEDGAPAGDLTREPVDYPADRALRLQALARGDEGFVLGLAYSTQRGYGRTHPFVGEIRVGEVAVEFVPE
ELGFAVPLGRVRLTECQMVNQFKGTGTAPPQFTRGYGLSFGQSERKAMAMALVDRALRAEELGEPVASPAQDQEFVLAHC
DSLQATGFVEHLKLPHYVDFQAELELVRRLRAEFERASDAVEMQEAAE

Sequences:

>Translated_368_residues
MYVAVKGGEAAIDNAHRLLAETRRGDPALPELSVAQIREQMALLVDRVMTEGSLYDPDLAALALKQARGDVVEAVFLVRA
YRTTLPRFGASAPVDTGAMAVERRISATFKDLPGGQVLGPTFDYTHRLVDFALAAETASEPAAEAEPRPDAGTCPRVTDL
LARDGLIEPSPPEDGAPAGDLTREPVDYPADRALRLQALARGDEGFVLGLAYSTQRGYGRTHPFVGEIRVGEVAVEFVPE
ELGFAVPLGRVRLTECQMVNQFKGTGTAPPQFTRGYGLSFGQSERKAMAMALVDRALRAEELGEPVASPAQDQEFVLAHC
DSLQATGFVEHLKLPHYVDFQAELELVRRLRAEFERASDAVEMQEAAE
>Mature_368_residues
MYVAVKGGEAAIDNAHRLLAETRRGDPALPELSVAQIREQMALLVDRVMTEGSLYDPDLAALALKQARGDVVEAVFLVRA
YRTTLPRFGASAPVDTGAMAVERRISATFKDLPGGQVLGPTFDYTHRLVDFALAAETASEPAAEAEPRPDAGTCPRVTDL
LARDGLIEPSPPEDGAPAGDLTREPVDYPADRALRLQALARGDEGFVLGLAYSTQRGYGRTHPFVGEIRVGEVAVEFVPE
ELGFAVPLGRVRLTECQMVNQFKGTGTAPPQFTRGYGLSFGQSERKAMAMALVDRALRAEELGEPVASPAQDQEFVLAHC
DSLQATGFVEHLKLPHYVDFQAELELVRRLRAEFERASDAVEMQEAAE

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3626

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnI family [H]

Homologues:

Organism=Escherichia coli, GI1790537, Length=355, Percent_Identity=63.3802816901408, Blast_Score=431, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008773 [H]

Pfam domain/function: PF05861 PhnI [H]

EC number: NA

Molecular weight: Translated: 39903; Mature: 39903

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVAVKGGEAAIDNAHRLLAETRRGDPALPELSVAQIREQMALLVDRVMTEGSLYDPDLA
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHH
ALALKQARGDVVEAVFLVRAYRTTLPRFGASAPVDTGAMAVERRISATFKDLPGGQVLGP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECC
TFDYTHRLVDFALAAETASEPAAEAEPRPDAGTCPRVTDLLARDGLIEPSPPEDGAPAGD
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
LTREPVDYPADRALRLQALARGDEGFVLGLAYSTQRGYGRTHPFVGEIRVGEVAVEFVPE
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEEECHHHHHHHHH
ELGFAVPLGRVRLTECQMVNQFKGTGTAPPQFTRGYGLSFGQSERKAMAMALVDRALRAE
HHCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ELGEPVASPAQDQEFVLAHCDSLQATGFVEHLKLPHYVDFQAELELVRRLRAEFERASDA
HHCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHH
VEMQEAAE
HHHHHHCC
>Mature Secondary Structure
MYVAVKGGEAAIDNAHRLLAETRRGDPALPELSVAQIREQMALLVDRVMTEGSLYDPDLA
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHH
ALALKQARGDVVEAVFLVRAYRTTLPRFGASAPVDTGAMAVERRISATFKDLPGGQVLGP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECC
TFDYTHRLVDFALAAETASEPAAEAEPRPDAGTCPRVTDLLARDGLIEPSPPEDGAPAGD
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
LTREPVDYPADRALRLQALARGDEGFVLGLAYSTQRGYGRTHPFVGEIRVGEVAVEFVPE
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEEECHHHHHHHHH
ELGFAVPLGRVRLTECQMVNQFKGTGTAPPQFTRGYGLSFGQSERKAMAMALVDRALRAE
HHCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ELGEPVASPAQDQEFVLAHCDSLQATGFVEHLKLPHYVDFQAELELVRRLRAEFERASDA
HHCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHH
VEMQEAAE
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]