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The map label for this gene is livJ [C]

Identifier: 170750164

GI number: 170750164

Start: 4008098

End: 4009234

Strand: Reverse

Name: livJ [C]

Synonym: Mrad2831_3766

Alternate gene names: 170750164

Gene position: 4009234-4008098 (Counterclockwise)

Preceding gene: 170750165

Following gene: 170750162

Centisome position: 65.96

GC content: 71.86

Gene sequence:

>1137_bases
ATGAAGATCGGACGTTTCGTCCCCGCCCGCGGGCCGCTCCGGCCCGCGCTGATCGCCCTGAGCCTGTGTCTCGCGCTCCT
GCCGGCCTCCCGCGCGGCGGCCGACGTGGTGATCGGCGTGGCGGTTCCGCGGTCTGGCCCCTACGTGGCGGTCGGCGAGC
AGGTCCTGCGCGGCGTCGAGGCCGCGGCCCGGGACGCCAACGCCATGGGCGGCCTCGACGGCCAGCCCGTGACCCTGGAC
GTGCAGGACGATGCCTGCGACTCGAACACCGCGATGGCGGTGGCCAACCACTTCGTCCGGTCCAACGTCCGGCTCGTCGT
CGGCCATGTCTGCTCGAACGCCTCCATCGCGGCCTCCGACATCTACGCGCAGAACGGGGTGGTGATGATCACCGCGGCCT
CGGTGGCGGCGCGGCTCACCGACCGGGGATTGCCGAACATCTTCCGGGTCTGCGGCCGCGACGACGATCAGGCCCGCCTG
TCCGCCGCCGTGCTGGCCGAGCGGTTCCGCGACCGGAAGATCGCGCTGATCCAGGACCAGACGCCGGCGGCCCGCAGCCT
CGCCGCCGCCACCAAGGACAACCTCAACCGCATCGGCGTGAACGAGGCGCTGTTCCTGTCGTTCTCGCCGAGCGACGCCG
ACGACGCCGCCCTGGCCGACCGCCTCAAGAGCGCGGGGATCGAGGTCGTCTACTACGGCGGCGACGCCGCCGAGATGGGC
CGCCTCGTGAAGATCGCGGCGGACCGGGGCTTCCGCCCCCAATGGTTCGGCACCTCGGCGATCGCCACGCCGGACTTCGC
CAAGATCGCCGGGTCCGCGAGCAACGGCGTTCTGATGACCTTCTACCTCGACCCGAGCCGGCAGCCCGCCGCCGCGGCCG
TCGTGAAGGCCTTCAAGGCCGAGGGCGTCGATCCCACCGGCTCCACGATCTACGGGTACGCGGCCATGCAGGCCCTGGTG
GCGGCCGGCAACTTCGCCCACTCCACCGAGCCGAGGCCCATCGCGCAGGCGCTGCACACCGAGCGCTTCGATCTCGTGCT
CGGCACCGTCGGCTTCGACGCCAAGGGCGACGTCACCGCGCAGGGCTACGTGCTCTACGTGTGGAAGGACGGGAGCTTCA
CGCCGGCCGGCAAGTGA

Upstream 100 bases:

>100_bases
GTCCTCGCGTATGGGCACTGGGTGCGGGGCGGGATCGAGGCTACGGAGCCGCATCCGCCGTCGCGCCGACGTGTCGCGCA
AGGATGCCCAGAAGAGCCCG

Downstream 100 bases:

>100_bases
GGGGCGGGCTCCTCATCACAGGGCCTGCGCGGCGTCGACCTCCGCGCCGCGGCCGAGCCCGCGCAGCAGCTCGGCGAGAT
GCGGGCGAACCCGCAACTGC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKIGRFVPARGPLRPALIALSLCLALLPASRAAADVVIGVAVPRSGPYVAVGEQVLRGVEAAARDANAMGGLDGQPVTLD
VQDDACDSNTAMAVANHFVRSNVRLVVGHVCSNASIAASDIYAQNGVVMITAASVAARLTDRGLPNIFRVCGRDDDQARL
SAAVLAERFRDRKIALIQDQTPAARSLAAATKDNLNRIGVNEALFLSFSPSDADDAALADRLKSAGIEVVYYGGDAAEMG
RLVKIAADRGFRPQWFGTSAIATPDFAKIAGSASNGVLMTFYLDPSRQPAAAAVVKAFKAEGVDPTGSTIYGYAAMQALV
AAGNFAHSTEPRPIAQALHTERFDLVLGTVGFDAKGDVTAQGYVLYVWKDGSFTPAGK

Sequences:

>Translated_378_residues
MKIGRFVPARGPLRPALIALSLCLALLPASRAAADVVIGVAVPRSGPYVAVGEQVLRGVEAAARDANAMGGLDGQPVTLD
VQDDACDSNTAMAVANHFVRSNVRLVVGHVCSNASIAASDIYAQNGVVMITAASVAARLTDRGLPNIFRVCGRDDDQARL
SAAVLAERFRDRKIALIQDQTPAARSLAAATKDNLNRIGVNEALFLSFSPSDADDAALADRLKSAGIEVVYYGGDAAEMG
RLVKIAADRGFRPQWFGTSAIATPDFAKIAGSASNGVLMTFYLDPSRQPAAAAVVKAFKAEGVDPTGSTIYGYAAMQALV
AAGNFAHSTEPRPIAQALHTERFDLVLGTVGFDAKGDVTAQGYVLYVWKDGSFTPAGK
>Mature_378_residues
MKIGRFVPARGPLRPALIALSLCLALLPASRAAADVVIGVAVPRSGPYVAVGEQVLRGVEAAARDANAMGGLDGQPVTLD
VQDDACDSNTAMAVANHFVRSNVRLVVGHVCSNASIAASDIYAQNGVVMITAASVAARLTDRGLPNIFRVCGRDDDQARL
SAAVLAERFRDRKIALIQDQTPAARSLAAATKDNLNRIGVNEALFLSFSPSDADDAALADRLKSAGIEVVYYGGDAAEMG
RLVKIAADRGFRPQWFGTSAIATPDFAKIAGSASNGVLMTFYLDPSRQPAAAAVVKAFKAEGVDPTGSTIYGYAAMQALV
AAGNFAHSTEPRPIAQALHTERFDLVLGTVGFDAKGDVTAQGYVLYVWKDGSFTPAGK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=351, Percent_Identity=35.6125356125356, Blast_Score=217, Evalue=9e-58,
Organism=Escherichia coli, GI1789867, Length=362, Percent_Identity=33.4254143646409, Blast_Score=202, Evalue=3e-53,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39494; Mature: 39494

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGRFVPARGPLRPALIALSLCLALLPASRAAADVVIGVAVPRSGPYVAVGEQVLRGVE
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHH
AAARDANAMGGLDGQPVTLDVQDDACDSNTAMAVANHFVRSNVRLVVGHVCSNASIAASD
HHHHCCHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEHHH
IYAQNGVVMITAASVAARLTDRGLPNIFRVCGRDDDQARLSAAVLAERFRDRKIALIQDQ
HEECCCEEEEEHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECC
TPAARSLAAATKDNLNRIGVNEALFLSFSPSDADDAALADRLKSAGIEVVYYGGDAAEMG
CCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCHHHHC
RLVKIAADRGFRPQWFGTSAIATPDFAKIAGSASNGVLMTFYLDPSRQPAAAAVVKAFKA
CEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
EGVDPTGSTIYGYAAMQALVAAGNFAHSTEPRPIAQALHTERFDLVLGTVGFDAKGDVTA
CCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEEEEEECCCCCCCEEE
QGYVLYVWKDGSFTPAGK
CCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKIGRFVPARGPLRPALIALSLCLALLPASRAAADVVIGVAVPRSGPYVAVGEQVLRGVE
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHH
AAARDANAMGGLDGQPVTLDVQDDACDSNTAMAVANHFVRSNVRLVVGHVCSNASIAASD
HHHHCCHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEHHH
IYAQNGVVMITAASVAARLTDRGLPNIFRVCGRDDDQARLSAAVLAERFRDRKIALIQDQ
HEECCCEEEEEHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECC
TPAARSLAAATKDNLNRIGVNEALFLSFSPSDADDAALADRLKSAGIEVVYYGGDAAEMG
CCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCHHHHC
RLVKIAADRGFRPQWFGTSAIATPDFAKIAGSASNGVLMTFYLDPSRQPAAAAVVKAFKA
CEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
EGVDPTGSTIYGYAAMQALVAAGNFAHSTEPRPIAQALHTERFDLVLGTVGFDAKGDVTA
CCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEEEEEECCCCCCCEEE
QGYVLYVWKDGSFTPAGK
CCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA