Definition Methylobacterium radiotolerans JCM 2831 chromosome, complete genome.
Accession NC_010505
Length 6,077,833

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The map label for this gene is fabG [C]

Identifier: 170750156

GI number: 170750156

Start: 3999428

End: 4000282

Strand: Reverse

Name: fabG [C]

Synonym: Mrad2831_3758

Alternate gene names: 170750156

Gene position: 4000282-3999428 (Counterclockwise)

Preceding gene: 170750157

Following gene: 170750153

Centisome position: 65.82

GC content: 73.8

Gene sequence:

>855_bases
GTGCGCGGCACGGTCTGCCTCGTCACCGGGGCGACCGGCGGCATCGGCTACGAGACGGCCCTCGGCCTCGCGCGCCTCGG
CGCGCGGGTCGCCATCGTCGGGCGGGACCCCGGGAAGCTGCAGGCCTGCGCCGACCGAATCCGCGCCGCGGTGCCCGGCG
CCGCCGTGGAGCCGCACCGCGCGGATCTGTCCGCGCAGGCCGAGATCCGCCGGCTCGCCGGGACCCTGCGGTCGGCCTAT
CCGCGCCTCGACGTGCTGGTGAACAATGCCGGCGCAATCTTCGACCGGCGCACCCTGACCGTCGACGGGATCGAGCGCAC
CTGGGCGCTCGACCATCTCGGCTACGTGCTCCTGACCCTCGAACTGCTCGACACGCTGAAGGAGTCGGCGGCGACGGGCG
GGAAGCCGCGGATCGTCAACGTCGCCTCCGCGGCCCACTACCGGGGGCACATCGACTTCGACGACGTCGAGGGCGCCCGG
CGGTACCGGGCGATGGGCGCGTATGCCCAGGCCAAGCTCGGGAACGTGCTGTTCACCTACGCGCTGGCCCGACGGCTGCG
GGGCAGCGGCATCACGGTGAACGCCGTCCATCCGGGGGTGGTCAATACCGGCTTCGCCAAGAACACCGGCGGGCTGCTCG
GCGCGGCCTGGGCGCTGATGCGGCCGTTCCTGATCACGCCCGAGAAGGGCGCGCGGACCTCCCTCCGCGCGGCCAGCGAT
CCGGGCCTGGACGGGGTCACCGGCCGATACTTCTCCCACGGGCGACCGAAGGTCTCCTCGGCCGAGAGCCGCGACGAGGC
CGTCCAGGAGCGGCTCTGGGCGCTGAGCCTCCGGCAGGTGGGCCGGAGCGACTGA

Upstream 100 bases:

>100_bases
TCGCTGGCCCGCATCCTGTTCGGCCTGTTCCTGCTCATCGCCATCGTGATCGTGGTCATCGCTTTCGCGGTCGGCCAGGC
GGTGTTCTGAGGCCGGCGCG

Downstream 100 bases:

>100_bases
CGCCCGACCGTTTCGCGGCGCCGGTCTCAGGCGACCGGCGGGTCGAGCAGCACCCAGAGCCGTCCGATCCTGCCGTCCAC
GATCTCGGCGACGTCCGTCC

Product: short-chain dehydrogenase/reductase SDR

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MRGTVCLVTGATGGIGYETALGLARLGARVAIVGRDPGKLQACADRIRAAVPGAAVEPHRADLSAQAEIRRLAGTLRSAY
PRLDVLVNNAGAIFDRRTLTVDGIERTWALDHLGYVLLTLELLDTLKESAATGGKPRIVNVASAAHYRGHIDFDDVEGAR
RYRAMGAYAQAKLGNVLFTYALARRLRGSGITVNAVHPGVVNTGFAKNTGGLLGAAWALMRPFLITPEKGARTSLRAASD
PGLDGVTGRYFSHGRPKVSSAESRDEAVQERLWALSLRQVGRSD

Sequences:

>Translated_284_residues
MRGTVCLVTGATGGIGYETALGLARLGARVAIVGRDPGKLQACADRIRAAVPGAAVEPHRADLSAQAEIRRLAGTLRSAY
PRLDVLVNNAGAIFDRRTLTVDGIERTWALDHLGYVLLTLELLDTLKESAATGGKPRIVNVASAAHYRGHIDFDDVEGAR
RYRAMGAYAQAKLGNVLFTYALARRLRGSGITVNAVHPGVVNTGFAKNTGGLLGAAWALMRPFLITPEKGARTSLRAASD
PGLDGVTGRYFSHGRPKVSSAESRDEAVQERLWALSLRQVGRSD
>Mature_284_residues
MRGTVCLVTGATGGIGYETALGLARLGARVAIVGRDPGKLQACADRIRAAVPGAAVEPHRADLSAQAEIRRLAGTLRSAY
PRLDVLVNNAGAIFDRRTLTVDGIERTWALDHLGYVLLTLELLDTLKESAATGGKPRIVNVASAAHYRGHIDFDDVEGAR
RYRAMGAYAQAKLGNVLFTYALARRLRGSGITVNAVHPGVVNTGFAKNTGGLLGAAWALMRPFLITPEKGARTSLRAASD
PGLDGVTGRYFSHGRPKVSSAESRDEAVQERLWALSLRQVGRSD

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI166795268, Length=279, Percent_Identity=41.2186379928315, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI186928839, Length=279, Percent_Identity=41.5770609318996, Blast_Score=184, Evalue=8e-47,
Organism=Homo sapiens, GI225579078, Length=289, Percent_Identity=41.8685121107266, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI193804850, Length=289, Percent_Identity=39.7923875432526, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI10190746, Length=301, Percent_Identity=37.5415282392027, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI19923931, Length=253, Percent_Identity=41.1067193675889, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI7706523, Length=286, Percent_Identity=34.2657342657343, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI146231950, Length=290, Percent_Identity=38.2758620689655, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI109715829, Length=210, Percent_Identity=28.0952380952381, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17558006, Length=293, Percent_Identity=33.4470989761092, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17532805, Length=298, Percent_Identity=30.2013422818792, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17532791, Length=290, Percent_Identity=27.9310344827586, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI193207620, Length=302, Percent_Identity=27.8145695364238, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI115533172, Length=278, Percent_Identity=30.2158273381295, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI115533174, Length=278, Percent_Identity=30.2158273381295, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17505416, Length=294, Percent_Identity=28.2312925170068, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71992402, Length=293, Percent_Identity=27.9863481228669, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI72001364, Length=289, Percent_Identity=28.719723183391, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=229, Percent_Identity=31.4410480349345, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17562908, Length=229, Percent_Identity=31.4410480349345, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI72000259, Length=213, Percent_Identity=31.924882629108, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17562906, Length=215, Percent_Identity=32.093023255814, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560150, Length=210, Percent_Identity=29.0476190476191, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17562990, Length=213, Percent_Identity=31.4553990610329, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17531453, Length=215, Percent_Identity=33.0232558139535, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17559104, Length=213, Percent_Identity=31.4553990610329, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17565030, Length=223, Percent_Identity=30.4932735426009, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17551412, Length=215, Percent_Identity=29.7674418604651, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI32565532, Length=211, Percent_Identity=27.9620853080569, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17538480, Length=219, Percent_Identity=29.2237442922374, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17562904, Length=212, Percent_Identity=30.6603773584906, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560332, Length=247, Percent_Identity=29.5546558704453, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17560220, Length=217, Percent_Identity=29.4930875576037, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17538486, Length=215, Percent_Identity=27.4418604651163, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6324820, Length=313, Percent_Identity=26.1980830670927, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI22024069, Length=284, Percent_Identity=41.9014084507042, Blast_Score=187, Evalue=7e-48,
Organism=Drosophila melanogaster, GI19921754, Length=292, Percent_Identity=39.041095890411, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24586328, Length=281, Percent_Identity=41.2811387900356, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28573296, Length=286, Percent_Identity=42.6573426573427, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI161076371, Length=279, Percent_Identity=38.3512544802867, Blast_Score=168, Evalue=4e-42,
Organism=Drosophila melanogaster, GI45554248, Length=254, Percent_Identity=42.1259842519685, Blast_Score=167, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24640117, Length=254, Percent_Identity=42.1259842519685, Blast_Score=167, Evalue=9e-42,
Organism=Drosophila melanogaster, GI45553409, Length=282, Percent_Identity=32.6241134751773, Blast_Score=144, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24647948, Length=282, Percent_Identity=32.6241134751773, Blast_Score=144, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24647946, Length=282, Percent_Identity=32.6241134751773, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI20129347, Length=285, Percent_Identity=32.9824561403509, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24656154, Length=275, Percent_Identity=31.6363636363636, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19922628, Length=275, Percent_Identity=31.6363636363636, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24647931, Length=305, Percent_Identity=31.1475409836066, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI28571526, Length=210, Percent_Identity=34.7619047619048, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21357041, Length=229, Percent_Identity=32.7510917030568, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24644339, Length=214, Percent_Identity=32.7102803738318, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI23397609, Length=212, Percent_Identity=31.1320754716981, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 30238; Mature: 30238

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MRGTVCLVTGATGGIGYETALGLARLGARVAIVGRDPGKLQACADRIRAAVPGAAVEPHR
CCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
ADLSAQAEIRRLAGTLRSAYPRLDVLVNNAGAIFDRRTLTVDGIERTWALDHLGYVLLTL
CCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCEEEECCCHHHHHHHHHHHHHHHH
ELLDTLKESAATGGKPRIVNVASAAHYRGHIDFDDVEGARRYRAMGAYAQAKLGNVLFTY
HHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALARRLRGSGITVNAVHPGVVNTGFAKNTGGLLGAAWALMRPFLITPEKGARTSLRAASD
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCCCCCC
PGLDGVTGRYFSHGRPKVSSAESRDEAVQERLWALSLRQVGRSD
CCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRGTVCLVTGATGGIGYETALGLARLGARVAIVGRDPGKLQACADRIRAAVPGAAVEPHR
CCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
ADLSAQAEIRRLAGTLRSAYPRLDVLVNNAGAIFDRRTLTVDGIERTWALDHLGYVLLTL
CCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCEEEECCCHHHHHHHHHHHHHHHH
ELLDTLKESAATGGKPRIVNVASAAHYRGHIDFDDVEGARRYRAMGAYAQAKLGNVLFTY
HHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALARRLRGSGITVNAVHPGVVNTGFAKNTGGLLGAAWALMRPFLITPEKGARTSLRAASD
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCCCCCC
PGLDGVTGRYFSHGRPKVSSAESRDEAVQERLWALSLRQVGRSD
CCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383668 [H]