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The map label for this gene is yafC [H]

Identifier: 170750153

GI number: 170750153

Start: 3997171

End: 3998127

Strand: Reverse

Name: yafC [H]

Synonym: Mrad2831_3755

Alternate gene names: 170750153

Gene position: 3998127-3997171 (Counterclockwise)

Preceding gene: 170750156

Following gene: 170750151

Centisome position: 65.78

GC content: 73.98

Gene sequence:

>957_bases
ATGGAACAGATCGGCCTCGACCGGCTGACGGGCATCGTCGCTTTCGCCCGCTCCGCCTCCCTCGGCAGCTACTCGGCGGC
CGCCCGGTCCCTCGGCATCTCGCCCTCGGCGGTGAGCAAGAGCGTGCAGCGGCTGGAGGCGCGGCTCGGCGTGAGCCTCT
TCACCCGCACGACGCGCTCGCTGACACTGACGCCCGAGGGGCGCGACCTGCAGGAGCGGGCGCTGCGACTCCTGCGCGAG
GCCGAGGCGATCGAGCAGGCCGCGGTCGCGGCGCGCTCCGAGCCTGCCGGCACCCTGAAGGTGGCGGCATCGATCCCGGT
CGGTGTGCACCTGCTCGGGCCGGCCCTGCCGCGGTTCCGCGCCCGCCATCCGAAGCTGTCGGTCGACCTGCGCCTGAGCG
ACCGCTTCGTGGACCTGATCGAGGAGGGCATCGACGTCGCGATCCGAGTCGGCGAACTCGCGGATTCCCGGCTGGTCTCG
CGGCGCCTCGCCCCGCACCGGCTCGGCGCCTTCGCCGCGCCCGTCTACCTGGCGCGGCGCGGCACGCCGCGGCACCCGGA
CGACCTCGCCCACCACGACTGCGTGAACTTCCGCTTCCAGAGCACGGGCCAGGCTCTGCGCTGGCCGTTCCAGGTCGGCG
GCCGCCGGATCGAGTTCACGCCGCATGCCGGCATCGTCGCGGATTTCAGCGACGCCGTCGCGGCGGTGCTGGTGGCCGGC
GGCGGCATCGGCATCTCGCCGACCTACATCGCCGCGCCGCACGTCGCCCGGGGGGCGCTCGTGCCGGTCCTGCAGGATTT
CGCGGTCGACCGCTCGGTCTTCACGGCGCTGTGGCCGGAGAGCCGGCGCGGCAATCCGAACGTGAAGGCGTTCCTCGCCT
TCCTCGACGAGGTGTTTCCGTCGCCGGCCCCCTGGGACGGCGTGCGCCCGCCGCCGATGCGCGGGGAGGATGCCTGA

Upstream 100 bases:

>100_bases
AACCTTGCCGCTCGGCGCCAGGGTGGCGGCGAGGGACACAAGATTGGAGCGGCGCCTTCGCCTTGATAATCGGGCCGCAA
TTCGTTTCAGTCATGCCGTC

Downstream 100 bases:

>100_bases
CAGAGATCCGCACTCGCCGGCGAGGCGGGCGTGCTCAGGCCGCCGCCCGCACCGTGTGATCGCCCTGCTGCTGGATCTCG
ATGAACACCCGGGTCACGTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MEQIGLDRLTGIVAFARSASLGSYSAAARSLGISPSAVSKSVQRLEARLGVSLFTRTTRSLTLTPEGRDLQERALRLLRE
AEAIEQAAVAARSEPAGTLKVAASIPVGVHLLGPALPRFRARHPKLSVDLRLSDRFVDLIEEGIDVAIRVGELADSRLVS
RRLAPHRLGAFAAPVYLARRGTPRHPDDLAHHDCVNFRFQSTGQALRWPFQVGGRRIEFTPHAGIVADFSDAVAAVLVAG
GGIGISPTYIAAPHVARGALVPVLQDFAVDRSVFTALWPESRRGNPNVKAFLAFLDEVFPSPAPWDGVRPPPMRGEDA

Sequences:

>Translated_318_residues
MEQIGLDRLTGIVAFARSASLGSYSAAARSLGISPSAVSKSVQRLEARLGVSLFTRTTRSLTLTPEGRDLQERALRLLRE
AEAIEQAAVAARSEPAGTLKVAASIPVGVHLLGPALPRFRARHPKLSVDLRLSDRFVDLIEEGIDVAIRVGELADSRLVS
RRLAPHRLGAFAAPVYLARRGTPRHPDDLAHHDCVNFRFQSTGQALRWPFQVGGRRIEFTPHAGIVADFSDAVAAVLVAG
GGIGISPTYIAAPHVARGALVPVLQDFAVDRSVFTALWPESRRGNPNVKAFLAFLDEVFPSPAPWDGVRPPPMRGEDA
>Mature_318_residues
MEQIGLDRLTGIVAFARSASLGSYSAAARSLGISPSAVSKSVQRLEARLGVSLFTRTTRSLTLTPEGRDLQERALRLLRE
AEAIEQAAVAARSEPAGTLKVAASIPVGVHLLGPALPRFRARHPKLSVDLRLSDRFVDLIEEGIDVAIRVGELADSRLVS
RRLAPHRLGAFAAPVYLARRGTPRHPDDLAHHDCVNFRFQSTGQALRWPFQVGGRRIEFTPHAGIVADFSDAVAAVLVAG
GGIGISPTYIAAPHVARGALVPVLQDFAVDRSVFTALWPESRRGNPNVKAFLAFLDEVFPSPAPWDGVRPPPMRGEDA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=34.9480968858132, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=32.1799307958478, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1789639, Length=295, Percent_Identity=31.864406779661, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI87081978, Length=301, Percent_Identity=29.5681063122924, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=25.6140350877193, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI145693193, Length=302, Percent_Identity=28.476821192053, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=25.5972696245734, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=25.1700680272109, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=26.2068965517241, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790011, Length=271, Percent_Identity=25.0922509225092, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786448, Length=255, Percent_Identity=28.6274509803922, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788481, Length=176, Percent_Identity=31.25, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082132, Length=285, Percent_Identity=24.5614035087719, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=30.1369863013699, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786508, Length=75, Percent_Identity=44, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790208, Length=126, Percent_Identity=31.7460317460317, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI157672245, Length=140, Percent_Identity=31.4285714285714, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34243; Mature: 34243

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQIGLDRLTGIVAFARSASLGSYSAAARSLGISPSAVSKSVQRLEARLGVSLFTRTTRS
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
LTLTPEGRDLQERALRLLREAEAIEQAAVAARSEPAGTLKVAASIPVGVHLLGPALPRFR
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHCCCCHHHH
ARHPKLSVDLRLSDRFVDLIEEGIDVAIRVGELADSRLVSRRLAPHRLGAFAAPVYLARR
CCCCCEEEEEEEHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
GTPRHPDDLAHHDCVNFRFQSTGQALRWPFQVGGRRIEFTPHAGIVADFSDAVAAVLVAG
CCCCCCHHHHHHHHHCEEECCCCCCEECHHHHCCEEEEECCCCCCCHHHHHHHHHHHHCC
GGIGISPTYIAAPHVARGALVPVLQDFAVDRSVFTALWPESRRGNPNVKAFLAFLDEVFP
CCCCCCCCEEECCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
SPAPWDGVRPPPMRGEDA
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEQIGLDRLTGIVAFARSASLGSYSAAARSLGISPSAVSKSVQRLEARLGVSLFTRTTRS
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
LTLTPEGRDLQERALRLLREAEAIEQAAVAARSEPAGTLKVAASIPVGVHLLGPALPRFR
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHCCCCHHHH
ARHPKLSVDLRLSDRFVDLIEEGIDVAIRVGELADSRLVSRRLAPHRLGAFAAPVYLARR
CCCCCEEEEEEEHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
GTPRHPDDLAHHDCVNFRFQSTGQALRWPFQVGGRRIEFTPHAGIVADFSDAVAAVLVAG
CCCCCCHHHHHHHHHCEEECCCCCCEECHHHHCCEEEEECCCCCCCHHHHHHHHHHHHCC
GGIGISPTYIAAPHVARGALVPVLQDFAVDRSVFTALWPESRRGNPNVKAFLAFLDEVFP
CCCCCCCCEEECCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
SPAPWDGVRPPPMRGEDA
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]