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The map label for this gene is truB [H]

Identifier: 170750123

GI number: 170750123

Start: 3964691

End: 3965719

Strand: Reverse

Name: truB [H]

Synonym: Mrad2831_3725

Alternate gene names: 170750123

Gene position: 3965719-3964691 (Counterclockwise)

Preceding gene: 170750124

Following gene: 170750122

Centisome position: 65.25

GC content: 74.44

Gene sequence:

>1029_bases
ATGACGGTTTCCATGGAAGATTTGCCGCCGGCCGAGCGCCAGGCTCCCCTGCCGAGCGCTGAGCGCCGCGGGCCGGAGGG
GCGCCGTCCTCAGCGCGGGCCGCGTCCCGACCGGCCGAAGCGCCGGGACGTCTCGGGCTGGGTGATCCTCGACAAGCATG
TCGGCATGACTTCGACGCACGCGGTCGCGGTGGTGAAGCGCGCCTTCAACGCCAAGAAGGCCGGCCATGCCGGCACCCTC
GACCCGCTGGCCTCCGGCATCCTGCCGATCGCCCTCGGCGAGGCGACCAAGACCGTCCCGTTCGTGATGGACGGCCGCAA
GGCCTACCGGTTCACCGTCCAGTGGGGCATCGAGACCGACACCGACGATGCCGAGGGGCGCCCCGTCGCGACGAGCGATG
CCCGCCCGGACCGGGCGGCGGTCGAGGCGGCGCTGCCGACCTTCGTGGGGGCGATCGAGCAGGTCCCGCCGCGCTACTCC
GCCATCAAGATCCAGGGCGAGCGTGCCTACGACCTCGCCCGCGAAGGCGAGACCGTGGAGCTGGTCGCCCGGCCAGTGCA
GATCGACCGGCTCGCGGTGGTCGAGCACGACGGCGAGCGCACGGTGATCGAGGCCGAGTGCGGCAAGGGCACCTACGTGC
GGGCGCTGGCCCGCGACCTCGGGCGGATGCTCGGCTGCTACGGCCACGTGGCCGCCCTGCGCCGGACCCGCGTCGGGCCC
TTCTCGGAGGCCGAGGCCTGCGCGGTCTCGGCCCTGACCGACGGCAGCCCCGAGGCCGCCCTCGCCTACCTGCGCCCCGT
GGAGACGGCCCTCGACTCCATCCCTGAGGTCGCGGTGTCGCGCGATCTGGGCCTGCGCCTGATGCGGGGGCAGCCGGTCA
TCCTGCGGGGCCGCGACGCCCCCGTCGAGGGCAAGGCCTTCGCGACCTGCGCCGGCATCCTGGTCGCCGTGGGCGACGTG
GAGCGCGGCGAGCTGATCCCGCACCGGGTCTTCCACCTCGGCGGGACGGCCCCCGGCCGCAGCGCCTGA

Upstream 100 bases:

>100_bases
CTCCGCGCCCGAGGACGACGAACCGGAGACCGGGACGGGGCATTGAGTCCCGATACCAAGCAGAAAGGGACGGGGCATCG
ACCCCGGGGGACACGCGGAC

Downstream 100 bases:

>100_bases
CATCCCGGCTCCGCGCCGCCCGGGCGGGCGGGCGCGGGGCTCCGGCAGGTGCAGGTCGGGCTGAGACGTGAGCGCGCAGG
CGGAACGAACCTGAGCCGTG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTVSMEDLPPAERQAPLPSAERRGPEGRRPQRGPRPDRPKRRDVSGWVILDKHVGMTSTHAVAVVKRAFNAKKAGHAGTL
DPLASGILPIALGEATKTVPFVMDGRKAYRFTVQWGIETDTDDAEGRPVATSDARPDRAAVEAALPTFVGAIEQVPPRYS
AIKIQGERAYDLAREGETVELVARPVQIDRLAVVEHDGERTVIEAECGKGTYVRALARDLGRMLGCYGHVAALRRTRVGP
FSEAEACAVSALTDGSPEAALAYLRPVETALDSIPEVAVSRDLGLRLMRGQPVILRGRDAPVEGKAFATCAGILVAVGDV
ERGELIPHRVFHLGGTAPGRSA

Sequences:

>Translated_342_residues
MTVSMEDLPPAERQAPLPSAERRGPEGRRPQRGPRPDRPKRRDVSGWVILDKHVGMTSTHAVAVVKRAFNAKKAGHAGTL
DPLASGILPIALGEATKTVPFVMDGRKAYRFTVQWGIETDTDDAEGRPVATSDARPDRAAVEAALPTFVGAIEQVPPRYS
AIKIQGERAYDLAREGETVELVARPVQIDRLAVVEHDGERTVIEAECGKGTYVRALARDLGRMLGCYGHVAALRRTRVGP
FSEAEACAVSALTDGSPEAALAYLRPVETALDSIPEVAVSRDLGLRLMRGQPVILRGRDAPVEGKAFATCAGILVAVGDV
ERGELIPHRVFHLGGTAPGRSA
>Mature_341_residues
TVSMEDLPPAERQAPLPSAERRGPEGRRPQRGPRPDRPKRRDVSGWVILDKHVGMTSTHAVAVVKRAFNAKKAGHAGTLD
PLASGILPIALGEATKTVPFVMDGRKAYRFTVQWGIETDTDDAEGRPVATSDARPDRAAVEAALPTFVGAIEQVPPRYSA
IKIQGERAYDLAREGETVELVARPVQIDRLAVVEHDGERTVIEAECGKGTYVRALARDLGRMLGCYGHVAALRRTRVGPF
SEAEACAVSALTDGSPEAALAYLRPVETALDSIPEVAVSRDLGLRLMRGQPVILRGRDAPVEGKAFATCAGILVAVGDVE
RGELIPHRVFHLGGTAPGRSA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=185, Percent_Identity=37.2972972972973, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI2367200, Length=308, Percent_Identity=38.3116883116883, Blast_Score=191, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI17553978, Length=214, Percent_Identity=29.9065420560748, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323204, Length=219, Percent_Identity=31.9634703196347, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324037, Length=127, Percent_Identity=35.4330708661417, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281364189, Length=212, Percent_Identity=28.7735849056604, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281364187, Length=212, Percent_Identity=28.7735849056604, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281364185, Length=212, Percent_Identity=28.7735849056604, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281364183, Length=212, Percent_Identity=28.7735849056604, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI62471759, Length=212, Percent_Identity=28.7735849056604, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17975520, Length=212, Percent_Identity=28.7735849056604, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 36647; Mature: 36516

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSMEDLPPAERQAPLPSAERRGPEGRRPQRGPRPDRPKRRDVSGWVILDKHVGMTSTH
CCCCCCCCCCHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHH
AVAVVKRAFNAKKAGHAGTLDPLASGILPIALGEATKTVPFVMDGRKAYRFTVQWGIETD
HHHHHHHHHHHCCCCCCCCHHHHHHCCEEEEECCCCCCCCEEECCCEEEEEEEEECCCCC
TDDAEGRPVATSDARPDRAAVEAALPTFVGAIEQVPPRYSAIKIQGERAYDLAREGETVE
CCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCEEE
LVARPVQIDRLAVVEHDGERTVIEAECGKGTYVRALARDLGRMLGCYGHVAALRRTRVGP
EEECCEEEEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FSEAEACAVSALTDGSPEAALAYLRPVETALDSIPEVAVSRDLGLRLMRGQPVILRGRDA
CCCHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCC
PVEGKAFATCAGILVAVGDVERGELIPHRVFHLGGTAPGRSA
CCCCCHHHHHHHHHHEECCCCCCCCCCHHHEEECCCCCCCCC
>Mature Secondary Structure 
TVSMEDLPPAERQAPLPSAERRGPEGRRPQRGPRPDRPKRRDVSGWVILDKHVGMTSTH
CCCCCCCCCHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHH
AVAVVKRAFNAKKAGHAGTLDPLASGILPIALGEATKTVPFVMDGRKAYRFTVQWGIETD
HHHHHHHHHHHCCCCCCCCHHHHHHCCEEEEECCCCCCCCEEECCCEEEEEEEEECCCCC
TDDAEGRPVATSDARPDRAAVEAALPTFVGAIEQVPPRYSAIKIQGERAYDLAREGETVE
CCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCEEE
LVARPVQIDRLAVVEHDGERTVIEAECGKGTYVRALARDLGRMLGCYGHVAALRRTRVGP
EEECCEEEEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FSEAEACAVSALTDGSPEAALAYLRPVETALDSIPEVAVSRDLGLRLMRGQPVILRGRDA
CCCHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCC
PVEGKAFATCAGILVAVGDVERGELIPHRVFHLGGTAPGRSA
CCCCCHHHHHHHHHHEECCCCCCCCCCHHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]