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The map label for this gene is lpdA [H]

Identifier: 170750122

GI number: 170750122

Start: 3963171

End: 3964556

Strand: Reverse

Name: lpdA [H]

Synonym: Mrad2831_3724

Alternate gene names: 170750122

Gene position: 3964556-3963171 (Counterclockwise)

Preceding gene: 170750123

Following gene: 170750121

Centisome position: 65.23

GC content: 73.23

Gene sequence:

>1386_bases
ATGCGCGAGCTCGACTACGACGTGGCGGTGATTGGTGCCGGGACCGCCGGCATCGCGGCCCATCGCGCCGCGACGGCGGC
TGGGGCGCGTTCGGTGCTGATCGAGCGCGGACCCGGCGGGACGACCTGCGCGCGGGTCGGCTGCATGCCCTCGAAGCTCC
TGATCGCCGCGGGCGCTGCCGCCCACGCGGCGCGCACCGCGGACCTGTTCGGCATCCGGGTTCCGGAGGTCGCGGTGGAC
GGGCCCGCCGTGCTGCGGCGCCTCCGCGCGGAGCGCGACCGGTTCGTCGGCAGTGTCCTCGACGGCCTCGACGATCTCCC
CGAGGGCGAGCGCCTCGACGGCACGGCCCGGTTCGAGGACGACCGTACCCTCCTGGTCGACGACGCGACCCGGATCCGCT
TCCGGGCGGCGGTCATCGCCACGGGATCGAGCCCGATCGTCCCCGGGCCTCTGCGCGGCCTCGGCGCGTTCGTCCTCACC
ACCGACACCCTCTTCGAGATCCCCGACCTCCCGGCTTCGCTGGCGGTCCTCGGTGCCGGAGCCGTCGGAATCGAGATCGC
GCAGGCGATGGCGCGGCTCGGCGTCGCCGTCACGGTGATCGACGCGGGCGACACCGTCGCCGGCCTGACGGAGCCCGACC
TCGCCGGGCAGGCCGCCGCGATCTTCGGCGCGGAGCTGGATCTCCACCTCGGCGCCGAGCTTCGGAAGGCCGTTCCGGAA
GGCGACGGCGTGTGTCTCCACTGGACCGACCGCGCCGGCCGCGAGCGGACCACGCGGGCGGCGCGGGTGCTGGCCGCCGC
CGGCCGTTCTCCCAATCTGGAGGCCCTCCGGCTCGAGGCGACCGGTCTATGCCTCGAGCCCGAGGGCCTGCCGGCTTTCG
ACCGGCGCAGCCTCGTCTGCGCGGGGGCACCGATCCTGATCGCGGGCGACGTCGACGGCTGGAGGCCGGTGCTGCACGAG
GCGAGCCGCCAGGGTGGGATCGCGGGTGCCAACGCGGCAGCCCTCGCGGCGGGCCGCCCGGTCGCGACCCCGGAGCCGTG
GACGCGCCTCGCGATGGTCTTCACCCATCCCCAGGCGGCGGTGATCGGCGCGCCCTACGACCCGGAGGACGATCGACGGA
TTGTCGGCGCCCTGGATTTCTCGGATCAGGGGCGCGCCCGCGTGGAGGGGGTGAACCGCGGCGGCATGCGGATCTGGGCC
GATCGGTCCGGGCGACTGCTCGGCGGCGAGATGCTCGGGCCGGCGGTGGAACACCTTGCGCACCTGCTCACCTTCGCCGT
CGCCGACGAGAAAACGGCCCAGGCCGTGCATGGCCGGCCGGTATATCATCCTACGGTTGAAGAAGGGCTTACTAGTATTA
TGTCTAAGATGATACAGAGAAGCTGA

Upstream 100 bases:

>100_bases
CGGGGCTCCGGCAGGTGCAGGTCGGGCTGAGACGTGAGCGCGCAGGCGGAACGAACCTGAGCCGTGACGCTTTGGCCCCG
GCGGACGGTCCGGGATGCTG

Downstream 100 bases:

>100_bases
GGCGTTGATCTTGCCGATATCACAAGCATTGCGCTCGACCATTTCGGTCGCCATACCCCGCTGAATACGCCGGTTTCGTT
CCTGATGCCGCCCAGTCAGA

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MRELDYDVAVIGAGTAGIAAHRAATAAGARSVLIERGPGGTTCARVGCMPSKLLIAAGAAAHAARTADLFGIRVPEVAVD
GPAVLRRLRAERDRFVGSVLDGLDDLPEGERLDGTARFEDDRTLLVDDATRIRFRAAVIATGSSPIVPGPLRGLGAFVLT
TDTLFEIPDLPASLAVLGAGAVGIEIAQAMARLGVAVTVIDAGDTVAGLTEPDLAGQAAAIFGAELDLHLGAELRKAVPE
GDGVCLHWTDRAGRERTTRAARVLAAAGRSPNLEALRLEATGLCLEPEGLPAFDRRSLVCAGAPILIAGDVDGWRPVLHE
ASRQGGIAGANAAALAAGRPVATPEPWTRLAMVFTHPQAAVIGAPYDPEDDRRIVGALDFSDQGRARVEGVNRGGMRIWA
DRSGRLLGGEMLGPAVEHLAHLLTFAVADEKTAQAVHGRPVYHPTVEEGLTSIMSKMIQRS

Sequences:

>Translated_461_residues
MRELDYDVAVIGAGTAGIAAHRAATAAGARSVLIERGPGGTTCARVGCMPSKLLIAAGAAAHAARTADLFGIRVPEVAVD
GPAVLRRLRAERDRFVGSVLDGLDDLPEGERLDGTARFEDDRTLLVDDATRIRFRAAVIATGSSPIVPGPLRGLGAFVLT
TDTLFEIPDLPASLAVLGAGAVGIEIAQAMARLGVAVTVIDAGDTVAGLTEPDLAGQAAAIFGAELDLHLGAELRKAVPE
GDGVCLHWTDRAGRERTTRAARVLAAAGRSPNLEALRLEATGLCLEPEGLPAFDRRSLVCAGAPILIAGDVDGWRPVLHE
ASRQGGIAGANAAALAAGRPVATPEPWTRLAMVFTHPQAAVIGAPYDPEDDRRIVGALDFSDQGRARVEGVNRGGMRIWA
DRSGRLLGGEMLGPAVEHLAHLLTFAVADEKTAQAVHGRPVYHPTVEEGLTSIMSKMIQRS
>Mature_461_residues
MRELDYDVAVIGAGTAGIAAHRAATAAGARSVLIERGPGGTTCARVGCMPSKLLIAAGAAAHAARTADLFGIRVPEVAVD
GPAVLRRLRAERDRFVGSVLDGLDDLPEGERLDGTARFEDDRTLLVDDATRIRFRAAVIATGSSPIVPGPLRGLGAFVLT
TDTLFEIPDLPASLAVLGAGAVGIEIAQAMARLGVAVTVIDAGDTVAGLTEPDLAGQAAAIFGAELDLHLGAELRKAVPE
GDGVCLHWTDRAGRERTTRAARVLAAAGRSPNLEALRLEATGLCLEPEGLPAFDRRSLVCAGAPILIAGDVDGWRPVLHE
ASRQGGIAGANAAALAAGRPVATPEPWTRLAMVFTHPQAAVIGAPYDPEDDRRIVGALDFSDQGRARVEGVNRGGMRIWA
DRSGRLLGGEMLGPAVEHLAHLLTFAVADEKTAQAVHGRPVYHPTVEEGLTSIMSKMIQRS

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=472, Percent_Identity=25.8474576271186, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI50301238, Length=497, Percent_Identity=23.1388329979879, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI22035672, Length=315, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1786307, Length=459, Percent_Identity=24.1830065359477, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI87081717, Length=471, Percent_Identity=25.4777070063694, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789915, Length=283, Percent_Identity=27.9151943462898, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI32565766, Length=467, Percent_Identity=24.1970021413276, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71983429, Length=381, Percent_Identity=28.0839895013123, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71983419, Length=381, Percent_Identity=28.0839895013123, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71982272, Length=226, Percent_Identity=28.3185840707965, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=473, Percent_Identity=24.3128964059197, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6325166, Length=389, Percent_Identity=25.9640102827763, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21358499, Length=472, Percent_Identity=25.8474576271186, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24640551, Length=346, Percent_Identity=27.7456647398844, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24640549, Length=346, Percent_Identity=27.7456647398844, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24640553, Length=346, Percent_Identity=27.7456647398844, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17737741, Length=305, Percent_Identity=27.5409836065574, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 47974; Mature: 47974

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRELDYDVAVIGAGTAGIAAHRAATAAGARSVLIERGPGGTTCARVGCMPSKLLIAAGAA
CCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHEECCCCCHHHEECCCH
AHAARTADLFGIRVPEVAVDGPAVLRRLRAERDRFVGSVLDGLDDLPEGERLDGTARFED
HHHHHHHHHHEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECC
DRTLLVDDATRIRFRAAVIATGSSPIVPGPLRGLGAFVLTTDTLFEIPDLPASLAVLGAG
CCEEEEECCCEEEEEEEEEECCCCCCCCCCHHHCEEEEEECCHHEECCCCCHHHHHHCCC
AVGIEIAQAMARLGVAVTVIDAGDTVAGLTEPDLAGQAAAIFGAELDLHLGAELRKAVPE
HHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECEEEEECCHHHHHHCCC
GDGVCLHWTDRAGRERTTRAARVLAAAGRSPNLEALRLEATGLCLEPEGLPAFDRRSLVC
CCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
AGAPILIAGDVDGWRPVLHEASRQGGIAGANAAALAAGRPVATPEPWTRLAMVFTHPQAA
ECCCEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCHHEEEEEEECCCEE
VIGAPYDPEDDRRIVGALDFSDQGRARVEGVNRGGMRIWADRSGRLLGGEMLGPAVEHLA
EEECCCCCCCCCEEEEEECCCCCCCCEEEECCCCCEEEEECCCCCEECCHHHHHHHHHHH
HLLTFAVADEKTAQAVHGRPVYHPTVEEGLTSIMSKMIQRS
HHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRELDYDVAVIGAGTAGIAAHRAATAAGARSVLIERGPGGTTCARVGCMPSKLLIAAGAA
CCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHEECCCCCHHHEECCCH
AHAARTADLFGIRVPEVAVDGPAVLRRLRAERDRFVGSVLDGLDDLPEGERLDGTARFED
HHHHHHHHHHEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECC
DRTLLVDDATRIRFRAAVIATGSSPIVPGPLRGLGAFVLTTDTLFEIPDLPASLAVLGAG
CCEEEEECCCEEEEEEEEEECCCCCCCCCCHHHCEEEEEECCHHEECCCCCHHHHHHCCC
AVGIEIAQAMARLGVAVTVIDAGDTVAGLTEPDLAGQAAAIFGAELDLHLGAELRKAVPE
HHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECEEEEECCHHHHHHCCC
GDGVCLHWTDRAGRERTTRAARVLAAAGRSPNLEALRLEATGLCLEPEGLPAFDRRSLVC
CCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
AGAPILIAGDVDGWRPVLHEASRQGGIAGANAAALAAGRPVATPEPWTRLAMVFTHPQAA
ECCCEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCHHEEEEEEECCCEE
VIGAPYDPEDDRRIVGALDFSDQGRARVEGVNRGGMRIWADRSGRLLGGEMLGPAVEHLA
EEECCCCCCCCCEEEEEECCCCCCCCEEEECCCCCEEEEECCCCCEECCHHHHHHHHHHH
HLLTFAVADEKTAQAVHGRPVYHPTVEEGLTSIMSKMIQRS
HHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339281; 1581034 [H]