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The map label for this gene is rpoH2 [H]

Identifier: 170750108

GI number: 170750108

Start: 3947891

End: 3948766

Strand: Direct

Name: rpoH2 [H]

Synonym: Mrad2831_3709

Alternate gene names: 170750108

Gene position: 3947891-3948766 (Clockwise)

Preceding gene: 170750107

Following gene: 170750111

Centisome position: 64.96

GC content: 70.09

Gene sequence:

>876_bases
ATGGCTGAGATCGCGGGAATTCGACGGCAGTTCATACGGACTGCCATGGAGGCACCCTTCCTGGCCCGGGAAGAAGAGCG
CGGCCTCGCCGTCCGCTGGAAGGACGAGCGCGACGAGCGGGCCCTCCACCGTCTGATCTCGGCCCATATGCGCCTCGTCA
TCGCCCTGGCGGGGCGTTTTCGTCACTACGGCCTGCCCATGGCCGATCTCGTCCAAGAAGGGCATGTCGGCCTGATGGAG
GCCGCGGCCCGGTTCGAGCCCGAGCGGGACGTCCGCTTCTCGACCTACGCGACGTGGTGGATCCGCGCCTCGATCCAGGA
CTACATCCTGCGCAACTGGTCGATCGTGCGCGGCGGCACGTCCTCCGCGCAGAAAGCCCTGTTCTTCAACCTGCGCCGGC
TGCGCGCCCGGCTGATGCAGTCCACCGAGGAGCATGTCGGCGACGAGATTCACCGCCGCATCGCCAGCGCCATCGGTGTC
TCCCGCGACGACGTCGCCCTGATGGACGCGCGCCTGTCCGGGCCCGACATGTCGCTCAACGCGCCCGTGGGCGAGGACAG
CGACGCCTCCTCCGAGCGGATGGACTTCCTCGTCGACGGCGCCGACCTGCCGGACGAGACCGTGAGCGCCACGGTCGACG
GCGAGCGTCGGTTGAGCTGGCTCCAGCAGGCCCTGACGGTCCTGTCGGAGCGCGAGCTGCGCATCCTGCGCGAGCGACGC
CTCGCCGAGGATCAGGCGACCCTGGAGGCCCTCGGCCAGCGGCTCGGCATCTCCAAGGAGCGCGTCCGCCAGATCGAGAA
CCGGGCCCTGGAGAAGCTGCGCCGCGCCCTGGCCGACCGCTTCCCCGCCGCCACGGGCAGCCCGCATATCGGCTGA

Upstream 100 bases:

>100_bases
CCTTCCGTTGTCGGTTGCCTGGCGTCGCCCCCCGGTGGCGCCTGCGCGTCGGGCTATTGGCTCGACGCCGCAACAGCACT
CGACAACAAGAGGCAGGCGG

Downstream 100 bases:

>100_bases
CCCTCGGTCGCGTCGCTGTCCCGAAGCCTGGCCCTGGGGCCGGGCTCCCGCATGTCAGACATCAGTCGTTCGGCGCCCCG
CCGTCCGGACGCGAGGCCGC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MAEIAGIRRQFIRTAMEAPFLAREEERGLAVRWKDERDERALHRLISAHMRLVIALAGRFRHYGLPMADLVQEGHVGLME
AAARFEPERDVRFSTYATWWIRASIQDYILRNWSIVRGGTSSAQKALFFNLRRLRARLMQSTEEHVGDEIHRRIASAIGV
SRDDVALMDARLSGPDMSLNAPVGEDSDASSERMDFLVDGADLPDETVSATVDGERRLSWLQQALTVLSERELRILRERR
LAEDQATLEALGQRLGISKERVRQIENRALEKLRRALADRFPAATGSPHIG

Sequences:

>Translated_291_residues
MAEIAGIRRQFIRTAMEAPFLAREEERGLAVRWKDERDERALHRLISAHMRLVIALAGRFRHYGLPMADLVQEGHVGLME
AAARFEPERDVRFSTYATWWIRASIQDYILRNWSIVRGGTSSAQKALFFNLRRLRARLMQSTEEHVGDEIHRRIASAIGV
SRDDVALMDARLSGPDMSLNAPVGEDSDASSERMDFLVDGADLPDETVSATVDGERRLSWLQQALTVLSERELRILRERR
LAEDQATLEALGQRLGISKERVRQIENRALEKLRRALADRFPAATGSPHIG
>Mature_290_residues
AEIAGIRRQFIRTAMEAPFLAREEERGLAVRWKDERDERALHRLISAHMRLVIALAGRFRHYGLPMADLVQEGHVGLMEA
AARFEPERDVRFSTYATWWIRASIQDYILRNWSIVRGGTSSAQKALFFNLRRLRARLMQSTEEHVGDEIHRRIASAIGVS
RDDVALMDARLSGPDMSLNAPVGEDSDASSERMDFLVDGADLPDETVSATVDGERRLSWLQQALTVLSERELRILRERRL
AEDQATLEALGQRLGISKERVRQIENRALEKLRRALADRFPAATGSPHIG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=280, Percent_Identity=38.9285714285714, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1789098, Length=265, Percent_Identity=33.2075471698113, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789448, Length=239, Percent_Identity=33.8912133891213, Blast_Score=108, Evalue=4e-25,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33049; Mature: 32918

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIAGIRRQFIRTAMEAPFLAREEERGLAVRWKDERDERALHRLISAHMRLVIALAGRF
CCHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RHYGLPMADLVQEGHVGLMEAAARFEPERDVRFSTYATWWIRASIQDYILRNWSIVRGGT
HHCCCCHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCC
SSAQKALFFNLRRLRARLMQSTEEHVGDEIHRRIASAIGVSRDDVALMDARLSGPDMSLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCC
APVGEDSDASSERMDFLVDGADLPDETVSATVDGERRLSWLQQALTVLSERELRILRERR
CCCCCCCCCCHHHHHHEECCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEDQATLEALGQRLGISKERVRQIENRALEKLRRALADRFPAATGSPHIG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AEIAGIRRQFIRTAMEAPFLAREEERGLAVRWKDERDERALHRLISAHMRLVIALAGRF
CHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RHYGLPMADLVQEGHVGLMEAAARFEPERDVRFSTYATWWIRASIQDYILRNWSIVRGGT
HHCCCCHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCC
SSAQKALFFNLRRLRARLMQSTEEHVGDEIHRRIASAIGVSRDDVALMDARLSGPDMSLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCC
APVGEDSDASSERMDFLVDGADLPDETVSATVDGERRLSWLQQALTVLSERELRILRERR
CCCCCCCCCCHHHHHHEECCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEDQATLEALGQRLGISKERVRQIENRALEKLRRALADRFPAATGSPHIG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606166 [H]