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The map label for this gene is mtkA [H]

Identifier: 170750065

GI number: 170750065

Start: 3897234

End: 3898412

Strand: Reverse

Name: mtkA [H]

Synonym: Mrad2831_3666

Alternate gene names: 170750065

Gene position: 3898412-3897234 (Counterclockwise)

Preceding gene: 170750066

Following gene: 170750064

Centisome position: 64.14

GC content: 69.3

Gene sequence:

>1179_bases
ATGGACGTCCACGAGTATCAGGCCAAGGAGCTGCTCGCGAGCTTCGGCGTGGCCGTCCCGAAAGGCGCGATCGCCTTCAG
CCCGGACCAAGCGGTCTACGCGGCGACCGAACTCGGCGGCTCGTTCTGGGCCGTGAAGGCGCAGATCCACGCGGGCGCCC
GCGGCAAGGCCGGCGGCATCAAGCTGTGCCGGACCTACAACGAGGTCCGCGACGCCGCCAAGGACCTCCTCGGCAAGCGC
CTCGTCACGCACCAGACCGGCCCCGAGGGCAAGCCGGTGCAGCGCGTCTACGTCGAGACCGCGGACCCGTTCGAGCGCGA
GCTCTACCTCGGCTACGTGCTGGACCGGAAGGCCGAGCGCGTCCGGGTCATCGCCAGCCAGCGCGGCGGCATGGACATCG
AGGAGATCGCCGCCAAGGAGCCGGATTCGCTCATCCAGGTGGTGGTCGAGCCGGCGGTCGGCCTGCAGCAGTTCCAGGCG
CGGGAGATCGCCTTCCAGCTCGGGCTGAACATCAAGCAGGTCTCGGCCGCGGTGAAGACGATCATGAACGCCTATCGGGC
GTTCCGCGACTGCGACGGCACCATGCTGGAGATCAATCCGCTCGTCGTGACCAAGGACGACCGGGTGCTGGCGCTCGACG
CCAAGATGAGCTTCGACGACAACGCCCTGTTCCGCCGGCCGGGCATCGCCGACATGCACGACCCGTCGCAGGGCGACCCC
CGCGAGGCCCAGGCCGCCGAGCACAACCTCAGCTACATCGGCCTCGAGGGCGAGATCGGCTGCATCGTCAACGGCGCCGG
GCTGGCCATGGCCACCATGGACATGATCAAGCACGCGGGCGGCGAGCCGGCGAACTTTTTGGATGTCGGCGGCGGTGCGT
CCCCTGAGCGGGTGGCGACGGCCTTCCGGCTCGTGCTCTCCGACCGCAACGTGAAGGCGATCCTGGTCAACATCTTCGCC
GGCATCAACCGCTGCGACTGGGTCGCCGAGGGCGTCGTCCAGGCCGCCCGCGAGGTGAAGATCGACGTGCCGCTGGTGGT
CCGGCTCGCCGGCACGAACGTGGAGGCGGGCAAGAAGATCCTGGCCGAGAGCGGCCTGGATCTCATCACCGCCGACACCC
TGGCGGAGGCGGCCAAGAAGGCGGTCGACGCCTGCGCCGCCGCCAAGACCCAGCACTGA

Upstream 100 bases:

>100_bases
TCGTCAAGCCCGAGATCAAAAAATTTGACGGGCGTAATCTTTCGACTCAACTAAAGTAGATGGCGTCAACGGCGGCAACG
AACCGCGGAGGAGGCGCCGA

Downstream 100 bases:

>100_bases
GAGGGGAGACCGCGCCATGAGCATCCTCATCGACGAGACCACCCCGATCATCGTCCAGGGCATCACGGGCGACAAGGGCA
CCTTCCACGCCAAGGAGATG

Product: malate--CoA ligase subunit beta

Products: NA

Alternate protein names: MTK-beta; Malate thiokinase; Malyl-CoA synthetase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MDVHEYQAKELLASFGVAVPKGAIAFSPDQAVYAATELGGSFWAVKAQIHAGARGKAGGIKLCRTYNEVRDAAKDLLGKR
LVTHQTGPEGKPVQRVYVETADPFERELYLGYVLDRKAERVRVIASQRGGMDIEEIAAKEPDSLIQVVVEPAVGLQQFQA
REIAFQLGLNIKQVSAAVKTIMNAYRAFRDCDGTMLEINPLVVTKDDRVLALDAKMSFDDNALFRRPGIADMHDPSQGDP
REAQAAEHNLSYIGLEGEIGCIVNGAGLAMATMDMIKHAGGEPANFLDVGGGASPERVATAFRLVLSDRNVKAILVNIFA
GINRCDWVAEGVVQAAREVKIDVPLVVRLAGTNVEAGKKILAESGLDLITADTLAEAAKKAVDACAAAKTQH

Sequences:

>Translated_392_residues
MDVHEYQAKELLASFGVAVPKGAIAFSPDQAVYAATELGGSFWAVKAQIHAGARGKAGGIKLCRTYNEVRDAAKDLLGKR
LVTHQTGPEGKPVQRVYVETADPFERELYLGYVLDRKAERVRVIASQRGGMDIEEIAAKEPDSLIQVVVEPAVGLQQFQA
REIAFQLGLNIKQVSAAVKTIMNAYRAFRDCDGTMLEINPLVVTKDDRVLALDAKMSFDDNALFRRPGIADMHDPSQGDP
REAQAAEHNLSYIGLEGEIGCIVNGAGLAMATMDMIKHAGGEPANFLDVGGGASPERVATAFRLVLSDRNVKAILVNIFA
GINRCDWVAEGVVQAAREVKIDVPLVVRLAGTNVEAGKKILAESGLDLITADTLAEAAKKAVDACAAAKTQH
>Mature_392_residues
MDVHEYQAKELLASFGVAVPKGAIAFSPDQAVYAATELGGSFWAVKAQIHAGARGKAGGIKLCRTYNEVRDAAKDLLGKR
LVTHQTGPEGKPVQRVYVETADPFERELYLGYVLDRKAERVRVIASQRGGMDIEEIAAKEPDSLIQVVVEPAVGLQQFQA
REIAFQLGLNIKQVSAAVKTIMNAYRAFRDCDGTMLEINPLVVTKDDRVLALDAKMSFDDNALFRRPGIADMHDPSQGDP
REAQAAEHNLSYIGLEGEIGCIVNGAGLAMATMDMIKHAGGEPANFLDVGGGASPERVATAFRLVLSDRNVKAILVNIFA
GINRCDWVAEGVVQAAREVKIDVPLVVRLAGTNVEAGKKILAESGLDLITADTLAEAAKKAVDACAAAKTQH

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0045

COG function: function code C; Succinyl-CoA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI11321583, Length=389, Percent_Identity=43.9588688946015, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI157779135, Length=378, Percent_Identity=42.8571428571429, Blast_Score=291, Evalue=5e-79,
Organism=Homo sapiens, GI294862256, Length=362, Percent_Identity=42.5414364640884, Blast_Score=276, Evalue=2e-74,
Organism=Escherichia coli, GI1786948, Length=384, Percent_Identity=50.78125, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17539378, Length=393, Percent_Identity=42.7480916030534, Blast_Score=311, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI17567829, Length=388, Percent_Identity=43.0412371134021, Blast_Score=309, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6321683, Length=393, Percent_Identity=43.765903307888, Blast_Score=342, Evalue=6e-95,
Organism=Drosophila melanogaster, GI21356231, Length=392, Percent_Identity=41.3265306122449, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI161078106, Length=389, Percent_Identity=40.6169665809769, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI24645208, Length=389, Percent_Identity=40.6169665809769, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI281361397, Length=393, Percent_Identity=40.4580152671756, Blast_Score=284, Evalue=7e-77,
Organism=Drosophila melanogaster, GI281361395, Length=393, Percent_Identity=40.4580152671756, Blast_Score=284, Evalue=7e-77,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2361 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013650
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR005811
- InterPro:   IPR017866
- InterPro:   IPR005809
- InterPro:   IPR016102 [H]

Pfam domain/function: PF08442 ATP-grasp_2; PF00549 Ligase_CoA [H]

EC number: =6.2.1.9 [H]

Molecular weight: Translated: 42032; Mature: 42032

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS01217 SUCCINYL_COA_LIG_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVHEYQAKELLASFGVAVPKGAIAFSPDQAVYAATELGGSFWAVKAQIHAGARGKAGGI
CCCHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEHHHCCCCEEEEEEEEECCCCCCCCCH
KLCRTYNEVRDAAKDLLGKRLVTHQTGPEGKPVQRVYVETADPFERELYLGYVLDRKAER
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHEEEHHHHHCHHHH
VRVIASQRGGMDIEEIAAKEPDSLIQVVVEPAVGLQQFQAREIAFQLGLNIKQVSAAVKT
HHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHH
IMNAYRAFRDCDGTMLEINPLVVTKDDRVLALDAKMSFDDNALFRRPGIADMHDPSQGDP
HHHHHHHHHCCCCCEEEECEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
REAQAAEHNLSYIGLEGEIGCIVNGAGLAMATMDMIKHAGGEPANFLDVGGGASPERVAT
CHHHHHHCCCEEEEECCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHH
AFRLVLSDRNVKAILVNIFAGINRCDWVAEGVVQAAREVKIDVPLVVRLAGTNVEAGKKI
HHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHHHH
LAESGLDLITADTLAEAAKKAVDACAAAKTQH
HHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDVHEYQAKELLASFGVAVPKGAIAFSPDQAVYAATELGGSFWAVKAQIHAGARGKAGGI
CCCHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEHHHCCCCEEEEEEEEECCCCCCCCCH
KLCRTYNEVRDAAKDLLGKRLVTHQTGPEGKPVQRVYVETADPFERELYLGYVLDRKAER
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHEEEHHHHHCHHHH
VRVIASQRGGMDIEEIAAKEPDSLIQVVVEPAVGLQQFQAREIAFQLGLNIKQVSAAVKT
HHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHH
IMNAYRAFRDCDGTMLEINPLVVTKDDRVLALDAKMSFDDNALFRRPGIADMHDPSQGDP
HHHHHHHHHCCCCCEEEECEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
REAQAAEHNLSYIGLEGEIGCIVNGAGLAMATMDMIKHAGGEPANFLDVGGGASPERVAT
CHHHHHHCCCEEEEECCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHH
AFRLVLSDRNVKAILVNIFAGINRCDWVAEGVVQAAREVKIDVPLVVRLAGTNVEAGKKI
HHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHHHH
LAESGLDLITADTLAEAAKKAVDACAAAKTQH
HHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961516 [H]